动物胆汁与肠内容物中弯曲菌带菌情况的观察

在受检的10种常见动物中发现,鹌鹑、猫、狗、猪、鸡、鸭和牛的胆汁中弯曲菌带菌率分别为23.64％、14.29％、7.14％、6.64％、5.88％、5.55％和3.63％。体外实验证明,弯曲菌在胆汁中可存活4～7周。动物肠内容物的带菌率普遍高于同种动物的胆汁带菌率。进一步提示,这些家畜、家禽是弯曲菌的重要传染源。     

深圳市市售家禽空肠弯曲菌带菌状况调查

目的了解深圳市当前三鸟市场鸡、鸭、鹅的空肠弯曲菌带菌情况,为进一步开展食品安全性研究,制定防控食源性空腑弯曲菌病的措施提供科学依据。方法从三鸟批发市场采取三鸟肛拭标本,依据GB/T4789．19—2003方法进行细菌检验。结果50份鸡肛拭子共检出空肠弯曲菌20株,阳性率为40％;20份鸭肛拭子共检出空肠弯曲菌2株,阳性率为10％;30份鹅肛拭子共检出空肠弯曲菌6株,阳性率为20％。检出的空肠弯曲菌均为空肠亚种。结论深圳市活禽空腑弯曲菌感染或带菌率较高,如果在销售或加工过程中卫生质量不良,会对消费者的健康构成潜在威胁。     

动物胆汁与肠内容物中弯曲菌菌情况的观察

在受检的１０种常见动物中发现，鹌鹑、狗、猪、鸡、鸭和胆汁中弯曲菌带菌率分别为２３．６４、１４、２９％、７．１４％、６．６４％、５．８８％、５．５５和３．６３％。体外实验证明，弯曲菌在胆汁中可存活４～７周。动物肠内容的带菌率普遍高于同种动物的胆汁带菌率。进一步提示，这些家畜、家禽是弯曲菌的重要传染源。     

凉山地区羊携带空肠弯曲菌的调查

空肠弯曲菌(Campyloacter fetus ssp jejuni)是人类急性感染性腹泻的常见和重要病原菌。国内外报告其检出率为5～14％,笔者在凉山彝族自治州的检出率为7.6％(13/170)。因家禽、家畜等多种动物是该菌的储存宿主,是人类空肠弯曲菌肠炎的重要传染源。我们对羊携带空肠弯曲菌的情况进行了调查分析。结果,在15。只羊盲肠内容物中分离培养并经过生化鉴定,检出了30株空肠弯曲菌,其带菌率为20物。用该地彝、汉杂居区急性感染性腹泻患者分离出的空肠弯曲菌与     

儿童中空肠弯曲菌感染情况的调查

本文报道了成都市及郊区农村529名9岁以下儿童和腹泻病儿室肠弯曲菌感染情况。城乡学龄前儿童的带菌率分别为0.6％和6.5％,二者差异显著(P<0.005)。腹泻病儿空肠弯曲菌阳性率为5.3％,不同年龄组中阳性率有显著差异(P<0.025)。9例空肠弯曲菌肠炎均是2岁以下婴幼儿。63名家庭成员中成人未分离出空肠弯曲菌。结果证实成都市城乡儿童中存在空肠弯曲菌感染。     

空肠弯曲菌肠炎的流行病学研究——主要贮存宿主的研究

为了阐明家禽家畜在人类空肠弯曲菌肠炎传染源中的作用,本文对疫源地内的鸡狗猫鸭猪进行了空肠弯曲菌带菌情况调查。并以经典的交叉吸收试验制备不同的血清型别,对患者和动物中分离的菌株进行了血清学研究,现将结果报导如下。     

一起幼儿园内空肠弯曲菌高感染情况的调查

作者报道了一起幼儿园内空肠弯曲菌高感染的传播。该幼儿园儿童空肠弯曲菌阳性率为23.0％(43/187)。腹泻病例空肠弯曲菌阳性率为18.6％(8/43),健康儿童带菌率为24.3％(35/144)。同期另一幼儿园健康儿童和门诊非腹泻患儿带菌率分别为11.3％(27/238)和7.5％(15/201),三者间差异显著(P<0.005)。通过流行病学调查及菌株的生物分型表明,传染源可能是两名患空肠弯曲菌肠炎的炊事员,他们与儿童的菌株为同一生物型。其传播可能是通过污染的食物或人与人接触而引起的。     

家禽家畜空肠弯曲杆菌带菌情况调查

空肠弯曲杆菌肠炎系人畜共患病。此病正常地寄生在许多动物,特别是鸟类的肠道中,家禽可能成为该菌的最主要的储存宿主,带菌或患病的家畜亦可构成传染源。本文仅就合肥地区家禽家畜带菌情况报告如下: 一、材料和方法选择作带菌调查的家禽家畜来自合肥市及所属县,计有市售鸡、鸭、鹅、猪、奶牛及收购的猫等6种动物。除奶牛、猪系采取新鲜粪便外,其余均使用肛拭采便。采便后立即保存于Cary-Blair半固体运送培养基内,4～12小时内接种于改     

桂林市学龄前期健康儿童携带胎儿弯曲菌空肠亚种的探讨

本文报导从桂林市3个月到56岁440名健康人群的粪便中,共分离培养出胎儿弯曲菌空肠亚种34株,正常人群的带菌率为7.7％。学龄前期儿童249人,带菌率为13.6％。分析了学龄前期儿童带菌率较高,而农村的学龄前期儿童带菌率又高于城市儿童的原因。     

食蟹猴弯曲菌感染调查

弯曲菌（Campylobacter）是1973年由Veton等确定的一个与人类疾病有关的新菌属。其中感染人和动物引起腹泻主要是空肠弯曲菌、大肠弯曲菌、胎儿弯曲菌等。该属菌在自然界分布较广，在家禽家畜以及野生动物肠道内带菌率很高，这些动物是弯曲菌的主要宿主。研究表明，实验动物肠道内也存在弯曲菌的感染。为了解养殖场饲养猴群弯曲菌的感染状况以及对猴群健康的影响，我们对本场饲养的食蟹猴群进行了多次弯曲菌检测，现将结果报告如下：     

广西地区主要家禽家畜携带空肠和结肠弯曲菌情况的调查

本文检查11种动物的肛拭和粪便标本共638只(份),检出空肠和结肠弯曲菌99株,其中鸡检出率33.83%,鸭25.51%,鸽11.90%,猪12.50%,奶牛4.76%,山羊9.09%,豚鼠66.66%.实验饲养的家兔、大白鼠、小白鼠和广西猕猴为阴性.以Skirrow氏生物分型法将99株菌分型:空肠弯曲菌生物Ⅰ型78株,结肠弯曲菌16株,NARTC 5株.1983年致南宁市许多儿童腹泻的空肠弯曲菌属于Skirrow生物Ⅰ型,说明家禽家畜携带空肠弯曲菌、结肠弯曲菌在流行病学上具有重要意义.     

空肠弯曲菌套式PCR快速检测方法的建立与初步应用

目的建立套式PCR快速检测实验动物空肠弯曲菌的方法。方法根据GenBank数据库的空肠弯曲菌flaA基因设计2对引物，对空肠弯曲菌抽提DNA作PCR扩增及克隆测序。同时，对其他病原菌抽提核酸扩增，并对120份临床样品进行检测。结果空肠弯曲菌经2对引物分别扩增出1719bp和640bp片段，与预期大小一致。套式PCR检测的最低限度为10CFU。整个检测过程可在8h内完成。而其他病原菌未出现特异性扩增条带。采用套式PCR对121份临床样品进行检测，检出31份阳性，与传统的细菌分离方法完全一致。结论本研究建立的套式PCR方法具有很好的特异性和敏感性，可用于实验动物空肠弯曲菌的快速检测。     

空肠弯曲菌外膜蛋白SDS—PAGE应用于流行病...

The characteristics of outer membrane protein profiles of 41 strains of Campylobacter jejuni from various sources by SDS-PAGE was studied. Seven and nine OMP patterns were differentiated respectively by the presence or absence of six outer membrane protein bands and by the number and size of the molecular weight of the major protein bands. Comparing the OMP patterns of the strains from human with those from animals, the authors inferred that chickens and other animals might be one of the sources for the human infection of Campylobacter jejuni in this district. A comparison between the OMP patterns of the strains from diarrheic children and those from healthy carriers suggested that the pathogenesis of Campylobacter jejuni be possibly associated with the outer membrane proteins. Using the techniques, the authors studied the infection of Campylobacter jejuni in a nursery. The result showed the infection was sporadic and of multi-sources, as evidenced by the multi-patterns of the outer membrane protein profiles. It also indicates that the person-to-person transmission plays a significant role in the infection of Campylobacter jejuni.     

Survey of Campylobacter jejuni/coli infection and risk factors in children

From March to May 1987, 798 children under 7 years of the outpatients in Chengdu district were examined and their stool samples cultured for Campylobacter jejuni/coli. C. jejuni/coli was isolated from 31 (9.51%) of 326 children with diarrhea and 22 (4.66%) of 472 children with non-diarrhea. The difference was significant (P less than 0.05). This result indicated that C. jejuni/coli is an important pathogen of children diarrhea in Chengdu district. Meanwhile, by inquiry we investigated risk factors related with C. jejuni/coli infections. Case group was consisted of 53 children with C. jejuni/coli positive. For each case, 3 controls were chosen from 745 children with C. jejuni/coli negative and matched according to age (less than 6 month), sex, living place (city or countryside), way of feeding, and whether to have diarrhea or not. The results has shown that contact with animals a week before being ill and habit of intake of food with hands are risk factors of C. jejuni/coli infection of childhood in Chengdu, with an odd ratio of 2.97 and 2.71 respectively. This provides valuable information about the prevention of C. jejuni/coli infection.     

空肠弯曲菌外膜蛋白SDS-PAGE应用于流行病学调查的研究

用SDS-PAGE分析不同来源41株空肠弯曲菌外膜蛋白特征,根据外膜蛋白图谱蛋白带带到组合情况及主带迁移率可将其各分为7个和9个类型.对人源和鸡等动物源菌株外膜蛋白类型的比较,提示鸡等动物是儿童空肠弯曲菌感染的传染源。对腹泻患儿和健康携带者菌株外膜蛋白类型的比较,提示空肠弯曲菌的致病性可能与其外膜蛋白特征有关.用此方法,作者分析了一起幼儿园空肠弯曲菌感染的流行病学关系,结果表明该感染呈散发多源性;人-人传播对空肠弯曲菌感染具有意义.     

Genetic and Antibiotic Resistance Characteristics of Campylobacter jejuni Isolated from Diarrheal Patients,Poultry and Cattle in Shenzhen

Objective To investigate genetic and antibiotic resistance characteristics of Campylobacter jejuni(C. jejuni) isolated from Shenzhen. Methods Multilocs sequence typing and agar dilution methods were used to define the genotype and antibiotic resistance of C. jejuni, respectively. Results In total, 126 C. jejuni strains were isolated. The prevalence of C. jejuni was 5.3% in diarrheal patients. The prevalence in poultry meat(36.5%) was higher than that in cattle meat(1.1%). However, the prevalence in poultry cloacal swabs(27.0%) was lower than that in cattle stool(57.3%). Sixty-two sequence types were obtained, among which 27 of the STs and 10 alleles were previously unreported. The most frequently observed clonal complexes were ST-21(11.9%), ST-22(10.3%), and ST-403(7.1%). ST-21, ST-45, ST-354, ST-403, and ST-443 complexes overlapped between isolates from patients and cattle, whereas ST-45 and ST-574 complexes overlapped between isolates from patients and poultry. All C. jejuni were resistant to at least one antibiotic. The highest resistance rate was toward ciprofloxacin(89.7%), followed by tetracycline(74.6%), and nalidixic acid(69.0%). Conclusion This is the first report of the genotypes and antibiotic resistance of C. jejuni in Shenzhen. Overlapping clonal complexes were found between isolates from patients and cattle, and between patients and poultry.     

广州地区食品分离空肠弯曲菌的病原及分子分型分析

目的了解广州地区食品中空肠弯曲菌的污染状况及菌株分子型别特征。方法采用传统生化鉴定、荧光免疫分析及PCR方法分离和鉴定所分离的菌株,用脉冲场凝胶电泳(PFGE)对菌株进行分子分型分析。结果从100份检材中分离到10株空肠弯曲菌,不同方法得到一致的鉴定结果。10株菌经PFGE分析共得到3个聚类群。结论传统生化鉴定与PCR方法相结合能有效地从食品中进行空肠弯曲菌的分离和鉴定,食品分离的空肠弯曲菌中存在PFGE型的多态性。     

深圳市龙岗区空肠弯曲菌的流行状况及分子分型

目的了解并分析深圳市龙岗区腹泻患者粪便、禽肉类等样品中空肠弯曲菌的流行状况及同源性。方法对2016年5—12月深圳市龙岗区某医院腹泻患者粪便或肛拭子样品和来自养鸡场、农贸市场、超市等地的禽畜类等样品进行空肠弯曲菌分离培养,菌株经镜检和生化鉴定后再使用脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)对其进行全基因组分子分型。最后应用Bio Numerics软件进行聚类分析。结果 41份腹泻患者样品分离出5株空肠弯曲菌,分离率为12.2%,全部阳性样品均来自11岁以下儿童。53份来自食品和外环境样品分离出14株空肠弯曲菌,分离率为26.4%,其中来自农贸市场的分离率为57.1%(8/14),来自不同超市样品空肠弯曲菌的分离率为11.1%(4/36)。18株菌呈现15种PFGE带型,其中来自市场1的3株菌株带型完全一致,且与病例1只有一条带的差异;来自市场2与病例5的带型完全一致且与病例4的带型只有一条带的差异。结论深圳市龙岗区腹泻患者空肠弯曲菌感染率较高,感染可能是由于食用了来自市场未煮熟的食物或接触了来自市场被空肠弯曲菌污染的食物,农贸市场存在空肠弯曲菌的交叉污染,该地区空肠弯曲菌呈现多样性。