革皮氏海参皂苷对环磷酰胺所致骨髓损伤模型小鼠造血作用的研究

目的研究革皮氏海参(Pearsonothuria graeffeii)皂苷对环磷酰胺所致骨髓损伤模型小鼠造血的调节作用。方法用不同剂量的革皮氏海参皂苷分别灌胃环磷酰胺所致骨髓损伤模型小鼠,采用实验血液学技术和造血祖细胞体外培养方法,检测小鼠外周血白细胞(WBC)、红细胞(RBC)、血小板(PLT)、血红蛋白(Hb)和网织红细胞(RET)数,骨髓有核细胞(BMNC),骨髓造血干细胞,粒单系祖细胞(CFU-GM),红系祖细胞(CFU-E)和巨核系祖细胞(CFU-Meg)数。结果革皮氏海参皂苷能提高小鼠外周血像中WBC、RBC、PLT、Hb和RET的数量,提高骨髓有核细胞数,促进造血干细胞的增殖和分化,增加骨髓中CFU-GM、CFU-E/CFU-E和CFU-Meg的集落生成。结论革皮氏海参皂苷对骨髓损伤模型小鼠有促进造血的作用。其作用途径可能通过调节机体的免疫功能,诱导机体产生多种造血细胞因子,促进造血干细胞的增殖,并诱导向粒单系、红系和巨核系祖细胞分化,恢复小鼠的造血功能。     

四物汤配方颗粒与四物汤对骨髓抑制小鼠造血损伤恢复作用的比较研究

[目的]观察四物汤配方颗粒与四物汤对骨髓抑制小鼠造血功能的影响，比较两者的功效。[方法]采用CO60γ照射和注射环磷酰胺复合制备骨髓抑制小鼠模型。应用实验血液学、造血祖细胞培养等技术方法探讨两组药对骨髓抑制小鼠外周血、骨髓有核细胞数、骨髓造血祖细胞的增殖的影响。[结果]四物汤配方颗粒与四物汤均能促进外周血、骨髓有核细胞数、骨髓造血祖细胞的增殖。但颗粒组比四物汤组在红细胞、血红蛋白、血小板以及红系，巨核系等项差异有统计学意义。[结论]四物汤配方颗粒与四物汤都能促进骨髓造血功能的恢复，但四物汤配方颗粒组有更显著的补血作用。     

四物汤配方颗粒对小鼠骨髓基质细胞造血功能的影响

目的 观察四物汤配方颗粒对骨髓抑制小鼠造血功能的影响,探讨其对抑制小鼠骨髓基质细胞内转化生长因子β1(TGF-β1)和细胞信号内转导分子果蝇MAD类似基因(Smads)表达影响.方法 将50只BALB/c小鼠随机分为正常组(A组)、模型组(B组)、3个不同剂量给药组(C、D、E组).除A组外其他组小鼠经2.0 Gy60Co γ照射后的第3天进行腹腔注射环磷酰胺(CTX,50 mg/kg),连续给药3 d后完成骨髓抑制模型制备.小鼠造模后,第2天开始给药,每日每只小鼠灌胃量20 ml/kg,A、B组灌入质量分数为0.9%生理氯化钠溶液,C、D、E组分别按四物汤颗粒剂的2.5、5.0、10.0g/kg溶于蒸馏水灌胃,共给药7 d.应用IMDM培养基培养骨髓基质细胞,免疫印迹法观察各组对骨髓基质细胞TGF-β1和Smads信号通路表达的影响.结果 ①TGF-β1的表达:与A组(0.463±0.075)比较,B组骨髓基质细胞表达显著升高(1.284±0.043,P<0.05);与B组比较,C、D、E组可使TGF-β1表达降低;②Smads的表达:与A组(0.973±0.032)比较,B组骨髓基质细胞表达显著升高(2.036±0.044,P<0.05);与B组比较,C、D、E组可使Smads表达降低.结论 四物汤配方颗粒可调节TGF-β1和Smads表达,对骨髓抑制小鼠造血微环境有一定改善.     

四物汤配方颗粒对骨髓抑制小鼠骨髓基质细胞某些物质表达的影响

目的 观察四物汤配方颗粒对骨髓抑制小鼠造血功能的影响,探讨其对造血微环境调控作用机制.方法 50只小鼠随机分成5组:正常组、模型组、3个不同剂量给药组.观察各组骨髓有核细胞(BMNC)对骨髓基质细胞(BM-SC)的黏附能力,应用免疫印迹法(WB)测定四物汤配方颗粒对骨髓抑制小鼠BMSC层粘连蛋白(LN)、纤维粘连蛋白(FN)、焦点黏附激酶(FAK)的影响.结果 四物汤配方颗粒各组均能明显促进LN、FN、FAK表达;并具有剂量依赖性,表现出高剂量组BMSC对正常骨髓细胞的黏附能力最明显、对LN、FN、FAK表达最高.结论 四物汤配方颗粒可通过对骨髓抑制小鼠造血微环境的改善,促进骨髓造血功能的恢复.     

乌贼墨制品促进小鼠造血功能的实验研究

目的:观察乌贼墨制品对小鼠造血功能的影响。方法:用不同剂量的乌贼墨制品分别灌胃正常小鼠和环磷酰胺所致骨髓造血损伤模型小鼠,采用造血祖细胞体外培养方法和实验血液学技术,检测小鼠骨髓有核细胞数、骨髓造血干细胞数、粒单系祖细胞数和外周血WBC、RBC,PLT,Hb和网织红细胞。结果:乌贼墨制品能够显著提高正常小鼠骨髓有核细胞、造血干细胞、粒单系祖细胞和外周血WBC数量;对环磷酰胺所致骨髓造血损伤有显著的拮抗作用和促进其恢复;对正常小鼠和模型小鼠外周血RBC,PLT,Hb和网织红细胞均无显著性影响。结论:乌贼墨制品能够促进骨髓造血。其作用途径可能通过调节机体的免疫机能,诱导产生多种细胞因子,促进造血干细胞、粒单系祖细胞的增殖,并向粒系分化,促进小鼠的粒系造血。     

刺五加对放射损伤骨髓微血管的保护作用

目的 观察刺五加注射液对放射损伤小鼠骨髓微血管的保护作用。方法 用^60Coγ射线辐射小鼠建立骨髓微血管放射损伤模型；6周龄雌性小鼠随机分为正常、模型、模型 刺五加注射液低剂量，模型 刺五加注射液高剂量和模型 复方丹参注射液5组。采用分光光度法分别测定各组小鼠股骨骨髓血红蛋白溶液标本的吸光度(A)值；鼠尾采血测定外周血红细胞、白细胞数量和血红蛋白含量；并分别观测骨髓毛细动脉根数、面积和骨髓造血组织容量百分率。结果 刺五加注射液能明显降低放射损伤小鼠骨髓血红蛋白含量，提高外周血红细胞和白细胞的数量以及增加骨髓毛细动脉根数、面积和骨髓造血组织容量百分率。结论 刺五加注射液能明显修复放射损伤小鼠骨髓微血管。保护骨髓造血微环境。     

重型再障患者血清粒细胞集落刺激因子水平与免疫抑制剂治疗关系的研究

免疫抑制剂治疗再生障碍性贫血(再障)取得显著疗效证明异常免疫介导在再障发病中起重要作用。其中细胞因子表达的异常与造血缺陷密切相关。粒细胞集落刺激因子(G—CSF)是造血细胞生长因子,具有调节骨髓祖细胞增殖、分化及成熟的功能~([1])。环孢霉素A(CsA)和抗淋巴细胞球蛋白(ALT)/抗T细胞球蛋白(ATG)均为免疫抑制剂,但其作用机制不同,两者结合治疗再障将提高有效率。为了进一步探讨重型再障(SAA)患者G—CSF水平与免疫抑制剂治疗的关     

抗VLA-4单克隆抗体动员的外周血干细胞重建辐射损伤小鼠造血的初步研究

目的 观察抗VLA-4单克隆抗体动员的外周血干细胞对辐射损伤小鼠造血重建的效果。方法 8.5 Gy~(60)Co γ射线照射的BALB/c小鼠,分别接受经生理盐水(实验1组)及抗VLA-4单克隆抗体(实验2组)动员的外周血干细胞移植,观察受体小鼠的4周存活率、外周血白细胞(WBC)、骨髓有核细胞(BMN),粒细胞巨噬细胞集落形成单位(CFU-GM)及脾集落形成单位(CFU-S)等指标。结果 实验2组小鼠的4周存活率、WBC、BMNC,CFU-GM、CFU-S数均明显高于对照组、实验1组,差异具有显著性(P<0.05或P<0.01)。结论 抗VLA-4单克隆抗体能有效动员小鼠外周血干细胞,并能成功重建辐射损伤小鼠的造血功能。     

c-Kit~+Lin~-细胞移植改善急性肝损伤的实验研究

目的探讨骨髓c-Kit+Lin-(CD117)细胞移植治疗小鼠急性肝损伤的效果以及机制,为骨髓源性肝干细胞的临床应用提供实验依据。方法 18只成年BALB/c小鼠随机分为正常组、实验组和干细胞移植组,正常组不予任何处理,模型组小鼠腹腔注射CCl4后仅输入生理盐水,移植组小鼠在腹腔注射CCl4后尾静脉输注骨髓CD117细胞(1×106),48 h后观察各组小鼠肝功能水平变化,苏木素-伊红(hematoxylin-eosin,HE)染色分析肝脏病理学改变,免疫组化检测肝脏细胞增殖情况。结果模型组腹腔注射CCl4后肝功能严重受损,血清谷丙转氨酶(alanine transaminase,ALT)、谷草转氨酶(glutamic oxalacetic transaminase,AST)、胆红素(bilirubin)等升高,肝脏的HE染色可见片状坏死,炎症细胞浸润;移植组小鼠移植干细胞48 h后小鼠肝功能有所恢复,病理提示肝脏坏死区域有所减小,Ki67免疫组化提示干细胞可促进肝脏再生。结论经尾静脉注入的骨髓来源的CD117干细胞能改善急性肝损伤肝功能以及修复受损肝脏,是治疗急性肝损伤的一种安全有效的方法。     

桑牛强生胶囊对4.0Gy照射小鼠造血功能的影响

目的 观察桑牛强生胶囊(SN)对4.0 Gy照射小鼠造血功能的保护作用。方法 将小鼠随机分为正常对照组、环磷酰胺对照组、阿归对照组和药物大小剂量5个实验组。实验组小鼠以⁶⁰Coγ射线照射和给予环磷酰胺注射形成骨髓抑制模型,药物组给予不同剂量中药口服,正常对照组与环磷酰胺对照组给予生理盐水。实验前及实验后测体重,第7、14天检测血象,停药后的第2天,处死小鼠,观察骨髓有核细胞数。结果 SN可使由于⁶⁰Coγ射线照射和环磷酰胺所致的Hb、RBC、WBC、PC减少明显提高,骨髓有核细胞数比环磷酰胺对照组明显增加,差异有统计学意义(P<0.05、P<0.01]。结论 SN有显著刺激骨髓造血功能的作用,具有防治辐射损伤的作用。     

咖啡酸对辐射损伤小鼠造血恢复作用的初步研究

目的 观察咖啡酸对辐射损伤小鼠造血恢复的影响。方法 建立接受4.5 Gy X射线照射的辐射损伤小鼠模型,BALB/c小鼠随机分为空白对照组,单纯照射组,CFA组,观察小鼠30天存活率、体重、外周血白细胞(WBC)、血红蛋白(HGB)、血小板(PLT)、骨髓有核细胞(BMNC)、粒细胞巨噬细胞集落形成单位(CFU-GM)、巨核细胞形成单位(CFU-MK)及脾集落形成单位(CFU-S)和脾脏指数(SI)等指标。结果 CFA组小鼠的30天存活率、体重、WBC、BMNC、CFU-GM、CFU-MK、CFU-S和SI数均明显高于单纯照射组,差异有统计学意义(P < 0.05或P < 0.01)。结论 CFA能促进辐射损伤小鼠的造血恢复。     

E838对辐射暴露小鼠造血功能的影响

目的 研究E838对辐射暴露小鼠造血功能的防护作用。方法 用内源性脾结节形成(CFU-S),股骨有核细胞(BMN)计数及脾脏指数等方法观察E838、炔雌醇、尼尔雌醇对¹³⁷Csγ射线照射7.5Gy小鼠造血功能的影响。观察受照小鼠的BMN、CFU-S和脾指数的变化。结果 照射后第9天E838各组的BMN数、CFU-S及脾脏指数与对照组相比有明显的增高,经统计学处理差异有显著性(P<0.001)。E838三个剂量组的BMN数与尼尔雌醇组比较有所上升,差异有显著性(P<0.001),与炔雌醇比较,中、高剂量组差异有显著性(P<0.05)。E838三个剂量组的脾脏指数与尼尔雌醇组和对照组相比较,差异有显著性(P<0.001),结论 E838对辐射暴露小鼠造血系统具有保护作用。     

苞叶雪莲水提物对小鼠辐射防护作用研究

目的 初步探讨苞叶雪莲水提物对小鼠辐射损伤的防护作用。方法 以辐射损伤小鼠作为研究对象,分别以不同剂量苞叶雪莲水提物予照射+给药组灌胃,而单纯照射组(IR组)灌相应量的生理盐水,灌胃5d后接受6Gy的X射线一次全身照射,然后再灌胃5d并继续观察,至第18天,测定体重、静脉血中的RBC、WBC、PLT、Hb含量、骨髓有核细胞数、骨髓DNA含量(A值)、脾重量、脾指数。结果 与IR组比较,各给药+照射组小鼠的体重、WBC、PLT、骨髓DNA含量(A值)、骨髓有核细胞数、脾重量、脾指数均明显升高,存在显著性统计学差异,P<0.01(血小板的IR+LD组、脾重量、脾指数P<0.05),各组均以IR+HD组升高最为明显;各给药+照射组小鼠的RBC、Hb含量与IR组比较无统计学差异,P>0.05。结论 苞叶雪莲水提物中的活性成分对小鼠的造血系统有明显的辐射防护作用,并能刺激小鼠脾细胞增殖,改善免疫功能。外周血RBC、Hb含量各照射组差异不明显,这可能与RBC、Hb代谢周期较长,造血组织对RBC释放、Hb合成延迟有关。     

Fluorosis and its hematological effects

Although it has been reported that fluoride ingestion has no influence on various indices of hematopoiesis, some research has been published that excessive fluoride developed anemia and eosinophilia of leukocytes. Isparta is situated on the lake region of Turkey where fluorosis is endemic. Our aim was to explore the hematological effects in rats induced by fluoride. In this study, Wistar-Albino rats were used, divided into two groups as control and fluorized. While the control group was administered commercial water (including 0.07 ppm fluoride), the fluorized group was administered 100 ppm fluoride in commercial drinking water for four months. At the end of four months, hematological indices (Hb, Hct, MCV, MCH, RDW, RBC, WBC, and platelet counts) were measured. In addition, bone marrow samples were investigated. Mean leukocyte counts (WBC) in the control group and fluorized group were 7.07 (2.62-12.25) and 2.76 (3.13-5.24)x 10(3)/mm3, respectively. We observed displastic changes on granulocytes in the bone marrow samples of the fluorized group. Although there were significant statistical changes in WBC, we did not determine red blood cell and platelet changes in the fluorized group.     

Chronic consumption of a moderately low protein diet does not alter hematopoietic processes in young adult mice

The current studies examined whether hematopoiesis in the bone marrow and T-cell development in the thymus were attenuated in young adult A/J mice fed a moderately low protein diet (MPD, 50 g protein/kg) for 15 wk compared with mice fed a control protein diet (CPD, 180 g protein/kg). Flow cytometric analyses using antibodies against CD31 and Ly-6C as well as CD4 and CD8 were performed to identify stem, mixed progenitor, erythroid, lymphoid, granuloid and monocytic compartments in the bone marrow and four thymocyte subsets, respectively. Chronic restriction of young adult mice to MPD neither decreased the cellularity nor altered the distribution of subpopulations in either primary tissue. Subsequently, a new set of mice were provided with CPD and a low protein diet (LPD, 25 g protein/kg). After 5 wk, body and thymus weights in LPD group were reduced 26 and 30%, respectively, which was accompanied by a 505% increase in serum corticosterone. Surprisingly, LPD did not alter the number or distribution of cells in the bone marrow and the percentages of thymocyte subsets, supporting the findings from the MPD group. We conclude that chronic consumption of a marginal protein diet by young adult mice does not disrupt hematopoietic processes.     

Study of the radio-protective effect of cuttlefish ink on hemopoietic injury

Irradiation leads to immunosuppression, hemopoiesis injury as well as sub-health of human being. The protective and therapeutic effects of cuttlefish ink on hemopoiesis in 60Co gamma radiated model mice were investigated. One hundred and twenty female ICR mice aged 6 weeks (20-24g) were randomly divided into five groups: the control group, the model group, and the low, medium, high dosage groups. The mice in different groups were orally administered normal solution (N.S.) or cuttlefish ink of different dosage daily for 40 days. Hemopoiesis impaired model was induced by 60Co gamma irradiating with lethal dose of 8.0 Gy. The number of bone marrow nucleated cells (BMNC), colony-forming unit in spleen (CFU-S), colony-forming unit of granulocyte and monocyte (CFU-GM), peripheral blood pictures and superoxide dismutase (SOD) activity in serum have been measured. Compared with model group, the decrease of BMNC, CFU-S, CFU-GM, peripheral leukocytes and SOD activity in serum in 60Co gamma irradiated mice of cuttlefish ink feeding groups were resisted significantly (p<0.05 or p<0.01). Moreover, the restoration of those indices was promoted significantly (p<0.05 or p<0.01). The cuttlefish ink showed no significant effect on peripheral erythrocytes, thrombocytes and hemoglobin. The results showed that cuttlefish ink had significant effects on granulopoiesis. The mechanism underlining these effects may be that the increase of antioxidant level in mice, the improvement of bone marrow haematopoietic microenvironment and the inducement of cellular factors promoted the proliferation and differentiation of CFU-S and CFU-GM and thus enhance the defensive system of organism.     

Radioprotective effects of Sipunculus nudus L. polysaccharide combined with WR-2721, rhIL-11 and rhG-CSF on radiation-injured mice

This study investigated the radioprotective effect of Sipunculus nudus L. polysaccharide (SNP) in combination with WR-2721, rhIL-11 and rhG-CSF on irradiated mice. A total of 70 Imprinting Control Region (ICR) mice were divided into seven groups: the control group, the model group and five administration groups. All groups, except the control group, were exposed to a 5 Gy ⁶⁰Co γ-ray beam. Blood parameters [including white blood cell (WBC), red blood cell (RBC) and platelet counts and hemoglobin level] were assessed three days before irradiation, and the on the 3rd, 7th and 14th days after irradiation. Spleen, thymus and testicular indices, DNA contents of bone marrow cells, bone marrow nucleated cells, sperm counts, superoxide dismutase (SOD), malondialdehyde (MDA), testosterone and estradiol levels in the serum were assessed on the 14th day after irradiation. The combined administration of SNP, WR-2721, rhIL-11 and rhG-CSF exerted synergistic recovery effects on peripheral blood WBC, RBC and platelet counts and hemoglobin levels in irradiated mice, and synergistic promotion effects on spleen, thymus, testicle, bone marrow nucleated cells and sperm counts in irradiated mice. The synergistic administration increased the serum SOD activities and serum testosterone content of irradiated mice, but synergy decreased the content of serum MDA and estradiol in irradiated mice. These results suggest that the combined administration of SNP, WR-2721, rhIL-11 and rhG-CSF should increase the efficacy of these drugs for acute radiation sickness, protect immunity, hematopoiesis and the reproductive organs of irradiated-damaged mice, and improve oxidation resistance in the body.