The effects of two levels of caffeine ingestion on excess postexercise oxygen consumption in untrained women

Summary The effects of two levels of caffeine ingestion (5 mg·kg ^–1, CAF1, and 10 mg·kg ^–1, CAF2) on postexercise oxygen consumption was investigated in six untrained women aged 20.5 (SEM 0.5) years. After a test to determine maximal oxygen consumption (VO_2max) each subject underwent three test sessions at 55% VO_2max either in a control condition (CON) or with the CAF1 or CAF2 dose of caffeine. During exercise, oxygen consumption was found to be significantly higher in the CAM and CAF2 trials, compared to CON (P<0.05). During the hour postexercise, oxygen consumption in CAF1 and CAF2 remained significantly higher than in CON (P<0.05). At all times throughout the exercise, free fatty acid (FFA) concentrations were significantly higher in the caffeine trials than in CON. The FFA concentrations 1 h postexercise (+ 60 min) were further elevated above resting values for all three trials. Caffeine ingestion caused the greatest elevation above resting levels being 1.89 (SEM 0.19) mmol·l^–1 and 1.96 (SEM 0.22) mmol·1^–1 for the CAF1 and CAF2 trials, respectively. This was significantly higher (P<0.0001) than the CON level which was 0.97 (SEM 0.19) mmol·l^–1. Respiratory exchange ratio (R) values became significantly lower (P<0.05) in CAF1 and CAF2 compared to CON at the onset of exercise and continued to decrease during the activity. Throughout the recovery period, R values were significantly lower for both caffeine trials compared to CON. The results of this study would suggest that caffeine is useful in significantly increasing metabolic rate above normal levels in untrained women during, as well as after, exercising at 55% VO_2max.     

The effects of intensity of exercise on excess postexercise oxygen consumption and energy expenditure in moderately trained men and women

Summary This experiment investigated the effects of intensity of exercise on excess postexercise oxygen consumption (EPOC) in eight trained men and eight women. Three exercise intensities were employed 40%, 50%, and 70% of the predetermined maximal oxygen consumption (VO_2max). All ventilation measured was undertaken with a standard, calibrated, open circuit spirometry system. No differences in the 40%, 50% and 70% VO_2max trials were observed among resting levels of oxygen consumption (V0₂) for either the men or the women. The men had significantly higher resting VO₂ values being 0.31 (SEM 0.01) 1·min^–1 than did the women, 0.26 (SEM 0.01) 1·min^–1 (P < 0.05). The results indicated that there were highly significant EPOC for both the men and the women during the 3-h postexercise period when compared with resting levels and that these were dependent upon the exercise intensity employed. The duration of EPOC differed between the men and the women but increased with exercise intensity: for the men 40% – 31.2 min; 50% – 42.1 min; and 70% – 47.6 min and for the women, 40% – 26.9 min; 50% – 35.6 min; and 70% – 39.1 min. The highest EPOC, in terms of both time and energy utilised was at 70% VO_2max. The regression equation for the men, where y=O₂ in litres, and x=exercise intensity as a percentage of maximum was y=0.380x + 1.9 (r ²=0.968) and for the women is y=0.374x–0.857 (r ²=0.825). These findings would indicate that the men and the women had to exercise at the same percentage of their VO_2max to achieve the maximal benefits in terms of energy expenditure and hence body mass loss. However, it was shown that a significant EPOC can be achieved at moderate to low exercise intensities but without the same body mass loss and energy expenditure.     

Effect of an acute period of resistance exercise on excess post-exercise oxygen consumption: implications for body mass management

Studies have shown metabolism to remain elevated for hours following resistance exercise, but none have gone beyond 16 h, nor have they followed a whole body, high intensity exercise protocol. To examine the duration of excess post-exercise oxygen consumption (EPOC) following a period of heavy resistance exercise, seven healthy men [mean (SD) age 22 (3) years, height 177 (8) cm, mass 83 (10) kg, percentage body fat 10.4 (4.2)%] engaged in a 31 min period of resistance exercise, consisting of four circuits of bench press, power cleans, and squats. Each set was performed using the subject's own predetermined ten-repetition maximum and continued until failure. Oxygen consumption ( ) measurements were obtained at consistent times (34 h pre-, 29 h pre-, 24 h pre-, 10 h pre-, 5 h pre-, immediately post-, 14 h post-, 19 h post-, 24 h post-, 38 h post-, 43 h post-, and 48 h post-exercise). Post-exercise measurements were compared to the baseline measurements made at the same time of day. The was significantly elevated (P<0.05) above baseline values at immediately post, 14, 19, and 38 h post-exercise. Mean daily values for both post-exercise days were also significantly elevated above the mean value for the baseline day. These results suggest that EPOC duration following resistance exercise extends well beyond the previously reported duration of 16 h. The duration and magnitude of the EPOC observed in this study indicates the importance of future research to examine a possible role for high intensity resistance training in a weight management program for various populations. Electronic Publication     

Comparison of oxygen kinetics in young and old subjects

Summary Five older men (aged 60–69 yr) and five young men (aged 21–29 yr) with approximately equal levels of age-corrected max were compared with respect to oxygen kinetics at equal absolute workloads (100 watts) and at equal relative workloads (45% max) on a cycle ergometer. At 45% max, half times for response to instantaneous transition from unloaded pedalling were 30.0 s and 27.4 s for old and young respectively (t=0.260,p<0.80). No significant differences were found in the response and by inference none existed in O₂ extraction. Mean half times for heart rate responses at a workload of 100 W were 24.2 s and 20.6 s for old and young groups respectively (t=0.722,p<0.49). Mechanical efficiency estimated from steady state data at 100 W was 19.8% and 20.5% for old and young groups respectively (t=0.574). The close similarity in responses to submaximal work in old and young subjects of equivalent fitness suggests caution in the interpretation of agewise decrements observed in physiological variables which may be sensitive to physical fitness status.     

The effects of protective clothing on energy consumption during different activities

Protective clothing (PPC) can have negative effects on worker performance. Currently little is known about the metabolic effects of PPC and previous work has been limited to a few garments and simple walking or stepping. This study investigated the effects of a wide range of PPC on energy consumption during different activities. It is hypothesized that wearing PPC would significantly increase metabolic rate, disproportionally to its weight, during walking, stepping and an obstacle course. Measuring a person’s oxygen consumption during work can give an indirect, but accurate estimate of energy expenditure (metabolic rate). Oxygen consumption was measured during the performance of continuous walking and stepping, and an obstacle course in 14 different PPC ensembles. Increases in perceived exertion and in metabolic rate (2.4–20.9%) when wearing a range of PPC garments compared to a control condition were seen, with increases above 10% being significant (P < 0.05). More than half of the increase could not be attributed to ensemble weight. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users. Statement: Energy expenditure is a crucial parameter in the assessment of heat and cold stress, calculation of requirements of food (expeditions, military) and air supplies (SCBA time limits). The observed effect of protective clothing (increases up to 21% in energy use) indicates that neglecting it may put workers at risk in extreme conditions.     

The effects of dietary manipulation upon the respiratory exchange ratio as a predictor of maximum oxygen uptake during fixed term maximal incremental exercise in man

Summary This study examined the effects of dietary manipulation upon the respiratory exchange ratio ( ) as a predictor of maximum oxygen uptake ( ). Seven healthy males performed fixed term maximal incremental treadmill exercise after an overnight fast on three separate occasions. The first test took place after the subjects had consumed their normal mixed diet (45±5% carbohydrate (CHO)) for a period of three days. This test protocol was then repeated after three days of a low CHO diet (3±2% CHO), and again after three days of a high CHO diet (61±5% CHO). Respiratory gases were continuously monitored during each test using an online system. No significant changes in mean exercise oxygen uptake ( ), or maximum functional heart rate (FHR_max) were found between tests. Mean exercise carbon dioxide output ( ) and R were significantly lower than normal after the low CHO diet (bothp<0.001) and significantly higher than normal after the high CHO diet (bothp<0.05). Moreover, compared with the normal CHO diet, the R-time relationship during exercise was at all times significantly (p<0.001) shifted to the right after the low CHO diet, and shifted to the left, being significantly so (p<0.05) over the final 5 min of exercise, after the high CHO diet. As a result, predictions of based on the R-time relationship were similar to recorded after the normal CHO dietary condition (-1.5±1.9%), but higher after the low CHO diet (+14.8±3.9%,p<0.001) and lower after the high CHO diet (–7.0±4.5%,p<0.01). These results indicate that dietary manipulation can significantly affect respiratory gas exchanges during fixed term maximal incremental exercise, and by doing so can significantly influence predictions of based on R.     

Influence of intensity fluctuation on exercise metabolism

This investigation was undertaken to examine the influence of intensity fluctuation on metabolic responses during and after exercise. Twenty-four males and 24 females were randomly assigned into one of the four groups consisting of 12 subjects of equal gender. Each group performed one of four 30-min exercise protocols: (1) cycling at a constant power output of 75 W (P1), (2) cycling with power output alternating between 50 and 100 W every 5 min (P2), (3) same as P2 except power output was alternated in a reverse order (P3), and (4) same as P2 except power output was alternated between 25 and 125 W (P4). Each exercise session was followed by a 25-min recovery and all protocols yielded the same mechanical work. Oxygen uptake (VO₂), heart rate (HR), respiratory exchange ratio (RER), and plasma lactate concentrations ([La]) were measured at rest and during exercise and recovery. Ratings of perceived exertion (RPE) were recorded during exercise only. During exercise, VO₂, HR and RPE did not differ across the four protocols. RER was higher (P < 0.05) in P4 than P1 and P2. [La] was higher (P < 0.05) in P4 than P1 and P3. During recovery, VO₂ were lower (P < 0.05) in P1 than P2, P3, and P4, while [La] was higher in P4 than P3. When the total workload was equated, intensity fluctuation exerted no added effect upon metabolic responses during exercise, but provoked greater energy expenditure following exercise. Reversing the order or increasing the magnitude of intensity fluctuation would not further alter metabolic consequences.     

The effect of exercise intensity and duration on the oxygen deficit and excess post-exercise oxygen consumption

Summary Nine males with mean maximal oxygen consumption ( ) =63.0 ml· kg^–1 · min^–1, SD 5.7 and mean body fat = 10.6%, SD 3.1 each completed nine counterbalanced treatments comprising 20, 50 and 80 min of treadmill exercise at 30, 50 and 70% . The OZ deficit, 8 h excess post-exercise oxygen consumption (EPOC) and EPOC:O₂ deficit ratio were calculated for all subjects relative to mean values obtained from 2 control days each lasting 9.3 h. The O₂ deficit, which was essentially independent of exercise duration, increased significantly (P<0.05) with intensity such that the overall mean values for the three 30%, 50% and 70% workloads were 0.83, 1.89 and 3.09 l, respectively. While there were no significant differences (P>0.05) between the three EPOCs after walking at 30% for 20 (1.01 l), 50 (1.43 l) and 80 min (1.041), respectively, the EPOC thereafter increased (P<0.05) with both intensity and duration such that the increments were much greater for the three 70% workloads (EPOC: 20 min=5.68 l; 50 min=10.04 l; 80 min= 14.59 l) than for the three 50% workload (EPOC: 20 min =3.14 l; 50 min=5.19 l; 80 min= 6.10 l). An analysis of variance indicated that exercise intensity was the major determinant of the EPOC since it explained five times more of the EPOC variance than either exercise duration or the intensity times duration interaction. The mean EPOC:O₂ deficit ratio ranged from 0.8 to 4.5 and generally increased with both exercise intensity and duration. These data imply that the EPOC is more than mere repayment of the O₂ deficit because metabolism is increasingly disturbed from resting levels as exercise intensity and duration increase due to other physiological factors occurring after the steady-state has been attained.     

The effects of cigarette smoking on maximal oxygen consumption and selected physiological responses of elite team sportsmen

Summary The acute and chronic effects of cigarette smoking on selected physiological responses were determined in seven well-trained non-smokers and seven well-trained habitual smokers. Non-smokers and smokers did not differ significantly with respect to maximal oxygen consumption ( ). The acute effect of smoking two cigarettes immediately prior to a graded exercise stress test on a treadmill ergometer did not significantly alter the of either group. However, the time taken for non-smokers to reach exhaustion decreased significantly (F=5.381, P<0.05) by a mean of 0.64 min. Smokers recorded lower scores for forced vital capacity (FVC) and forced expiratory volume in the 1st s exhalation (FEV₁) than non-smokers. Only the mean FVC of smokers recorded 5 min post-exercise was significantly altered by pre-exercise smoking. No differences were found between the resting heart rates (HR) of non-smokers and smokers. Smoking two cigarettes significantly (F=44.720, P<0.01) increased the mean resting HR of smokers and non-smokers by 15.8 beats·min^–1 and 15.6 beats·min^–1 respectively. No alteration to the exercise HR of either group was found under smoking conditions of the tests.     

Theoretical predictions of maximal oxygen consumption in hypoxia: effects of transport limitations

A Krogh-type model for oxygen transport is used to predict maximal oxygen consumption (V(.-) O(2max)) of human skeletal muscle under hypoxic conditions. Assumed values of capillary density, blood flow, and hemoglobin concentration are based on measurements under normoxic and hypoxic exercise conditions. Arterial partial pressure of oxygen is assumed to decrease with reductions in inspired partial pressure of oxygen (P(I)O(2)), as observed experimentally. As a result of limitations of convective and diffusive oxygen delivery, predicted V(.-) O(2max) values decline gradually as P(I)O(2) is reduced from 150 mmHg to about 80 mmHg, and more rapidly as P(I)O(2) is further reduced. At very low levels of P(I)O(2), V(.-) O(2max) is limited primarily by convective oxygen supply. Experimentally observed values of V(.-) O(2max) in hypoxia show significant dispersion, with some values close to predicted levels and others substantially lower. These results suggest that maximal oxygen consumption rates in hypoxia are not necessarily determined by oxygen transport limitations and may instead reflect reduced muscle oxygen demand.     

Resting oxygen consumption in rats during food restriction,starvation and refeeding

Oxygen consumption was measured in male rats during starvation and during different regimens of restricted feeding and refeeding after starvation. Changes in oxygen consumption and body mass were mostly parallel, but rats with a very reduced food intake displayed the same reduction in oxygen consumption as starved rats, despite the smaller reduction in body mass. Also, rats fed different amounts of food after starvation had different oxygen consumptions, but displayed the same changes in body mass. Two different refeeding regimens with restricted food amounts either induced a further depression of oxygen consumption (i.e. below starvation oxygen consumption), or a stabilizing of oxygen consumption on the level of starvation. The changes in oxygen consumption during restriction and feeding after starvation indicate that reductions in resting metabolic rate may not always be predicted from either body mass change or food intake.     

应用DATEX Ultima监测仪观察CO2气腹对机体呼吸功能和氧耗的影响

目的 :通过 Ultima监测仪观察 CO2 气腹对机体呼吸和氧耗有何影响。方法 :在气管内静吸全麻复合硬膜外麻醉下行 L C。以 Ultima监测仪持续监测呼吸功能和气体的变化。结果 :PETCO2 和 VCO2 在气腹 2 0 m in后较气腹前显著增加。 PAW在气腹开始后较气腹前显著升高 (P<0 .0 0 1)。 MVE在气腹后较气腹前降低 (P>0 .0 5 )。 VO2 在气腹后 10min时显著高于气腹前水平 (P<0 .0 1) ,以后继续升高。结论 :在 CO2 气腹期间 ,通过 Ultima监测仪能连续观察 PETCO2 、VT、MVE、PAW及 VO2 的动态变化。     

Effect of diet on muscle glycogen and blood glucose utilization during a short-term exercise in man

7 subjects were studied at rest and during a 6 min submaximal exercise (65% of Vo₂, max) on two occasions, the first preceded by a fat rich diet and the second by a carbohydrate rich diet. Oxygen uptake and respiratory exchange ratio (R) were measured at rest and heart rate both at rest and during exercise. Arterial-femoral venous differences for oxygen, glucose, lactate and β-hydroxybutyrate and arterial concentrations of free fatty acids were measured at rest and during exercise. Changes in muscle glycogen (in 6 subjects) and lactate concentration were determined by biopsies from m. quadriceps femoris taken before and immediately after exercise. Muscle glycogen decreased less during exercise after the fat than after the carbohydrate diet in 5 of the 6 subjects, whereas blood glucose extraction by the exercising legs did not change with diet. Muscle lactate accumulation and release were smaller after the fat diet. In conclusion, the muscle glycogen utilization during a short-term exercise appeared to be lower after the fat than after the carbohydrate diet, but not the concomitant blood glucose extraction. These differences between diets were similar to those observed after a more prolonged work at the same load.     

Acute hyperoxaemia‐induced effects on regional blood flow,oxygen consumption and central circulation in man

Aim: Despite numerous in vitro and animal studies, circulatory effects and mechanisms responsible for the vasoconstriction seen during hyperoxaemia are yet to be ascertained. The present study set out to: (i) set up a non‐invasive human model for the study of hyperoxia‐induced cardiovascular effects, (ii) describe the dynamics of this effect and (iii) determine whether hyperoxaemia also, by vasoconstriction alters oxygen consumption (O₂). Methods: The study comprised four experiments (A, B, C and D) on healthy volunteers examined before, during and after 100% oxygen breathing. A: Blood flow (mL min^?1·100 mL^?1 tissue), venous occlusion plethysmography was assessed (n = 12). B: Blood flow was recorded with increasing transcutaneous oxygen tension (P_tcO₂) levels (dose–response) (n = 8). C: Heart rate (HR), stroke volume, cardiac output (CO) and systemic vascular resistance (SVR) was assessed using echocardiography (n = 8). D: O₂ was measured using an open circuit technique when breathing an air‐O₂ mix (fraction of inhaled oxygen: F_iO₂ = 0.58) (n = 8). Results: Calf blood flow decreased 30% during O₂ breathing. The decrease in calf blood flow was found to be oxygen dose dependent. A similar magnitude, as for the peripheral circulation, of the effect on central parameters (HR/CO and SVR) and in the time relationship was noted. Hyperoxia did not change O₂. An average of 207 (93) mL O₂ per subject was washed in during the experiments. Conclusion: This model appears suitable for the investigation of O₂‐related effects on the central and peripheral circulation in man. Our findings, based on a more comprehensive (central/peripheral circulation examination) evaluation than earlier made, suggest significant circulatory effects of hyperoxia. Further studies are warranted to elucidate the underlying mechanisms.     

The effect of rest interval length on metabolic responses to the bench press exercise

The purpose of this study was to examine the effects of different rest interval (RI) lengths on metabolic responses to the bench press. Eight resistance-trained men performed 10 randomized protocols [five sets of bench press with 75 or 85% of 1RM for ten (10REP) and five repetitions (5REP), respectively, using different RI (30 s, 1, 2, 3, 5 min)]. Oxygen consumption (VO₂) was measured during exercise and for 30 min post exercise. For 30-s and 1-min RI: reductions (15–55%) in resistance and volume were observed (set 5 < 4 < 3 < 2 < 1). For 2-min RI: performance was maintained during the first two sets but was reduced by 8–29% during sets 3–5. For 3-min RI: a reduction was observed in volume where sets 4 and 5 were lower than sets 1–3 (∼21%). For 5-min RI: only a reduction in set 5 was observed. Mean VO₂ and ventilation (V _E) were progressively higher as RI length was shortened. VO₂ area under the curve indicated 10REP > 5REP for all RI except 1-min. Respiratory exchange ratio (RER) was elevated similarly for each protocol. Post exercise, VO₂, V _E, and RER were elevated through 30 min. No differences between RI were observed following 10REP; however, VO₂ after 30-s was higher than 2-, 3-, and 5-min and 1-min was higher than 5-min during 5REP. Fatigue rate was correlated (r = 0.30–0.49) to all metabolic variables. A continuum of performance reductions and metabolic responses were observed. The largest reductions in performance occurred with very short RI (<1 min), and performance was maintained during the first 3–4 sets when 3- and 5-min RI were used.     

17O MRS assesses the effect of mild hypothermia on oxygen consumption rate in tumors

Although oxygen consumption is a key factor in metabolic phenotyping, its assessment in tumors remains critical, as current technologies generally display poor specificity. The objectives of this study were to explore the feasibility of direct ¹⁷O nuclear magnetic resonance (NMR) spectroscopy to assess oxygen metabolism in tumors and its modulations. To investigate the impact of hypometabolism induction in the murine fibrosarcoma FSAII tumor model, we monitored the oxygen consumption of normothermic (37°C) and hypothermic (32°C) tumor‐bearing mice. Hypothermic animals showed an increase in tumor pO₂ (measured by electron paramagnetic resonance oximetry) contrary to normothermic animals. This was related to a decrease in oxygen consumption rate (assessed using ¹⁷O magnetic resonance spectroscopy (MRS) after the inhalation of ¹⁷O₂‐enriched gas). This study highlights the ability of direct ¹⁷O MRS to measure oxygen metabolism in tumors and modulations of tumor oxygen consumption rate.     

Effect of low oxygen inhalation on changes in blood pH, lactate, and ammonia due to exercise

The present study examined the effect of hypoxia-induced respiratory alkalosis on exercise-induced metabolic acidosis and increases in plasma lactate and ammonia levels. Six male subjects underwent exercise of increasing intensity until exhaustion: (1) in normoxia (20.9% O₂) (=MAX), (2) in hypoxia (12% O₂) (=HP) in which hypoxic condition had been maintained from 60 min before to 30 min after exercise, and (3) the same intensity of exercise as HP in normoxia (=SUB). Arterialized blood was drawn from a superficial vein. Post-exercise blood pH was significantly higher in HP than in MAX (P<0.05), although plasma lactate was at the same level. For hypoxia as compared to normoxia, regression analysis confirmed a parallel shift of plasma lactate to higher pH levels indicating the effect of respiratory alkalosis (P<0.01). After exercise plasma levels of ammonia were lower in HP than in MAX (P<0.05). Regression analysis between ammonia and pH revealed nearly identical changes in hypoxia and normoxia at low pH. From these results, we conclude that: (1) hypoxia-induced respiratory alkalosis attenuated exhaustive exercise-induced metabolic acidosis, (2) plasma lactate concentration was determined by the relative exercise intensity, (3) the maximum plasma ammonia concentration under exhaustive exercise was reduced at hypoxia because of respiratory alkalosis.     

不同排便体位对经皮冠状动脉介入术后患者心肌耗氧量的影响

目的探讨不同排便体位对经皮冠状动脉介入术（percutaneous coronary intervention,PCI）后患者心肌耗氧量的影响。方法选取PCI术后患者110例,随机分为平卧位组和半卧位组,采用不同体位排便,记录排便过程中心率、血压变化,并计算心肌耗氧量。结果平卧位组患者排便时间为（16.21±6.12）min,半卧位组排便时间为（11.12±5.82）min。平卧位组患者排便较半卧位组费力,P〈0.05。平卧位组患者便前准备和便中后期阶段心率及平均动脉压均高于半卧位组,代表心肌耗氧量的D-P值也高于半卧位组,P〈0.05。结论 PCI术后患者半卧位排便体位可减少排便过程中心肌耗氧量的增加,半卧位可考虑作为PCI术后患者床上排便方式的首选。     

The influence of training on oxygen isotope fractionation in healthy subjects

We determined the oxygen isotope fractionation in expired alveolar gas relative to inspired air (delta(A-I)) in eight young, healthy subjects at rest and at five levels of exercise up to maximal workload both before and after a training period of about 4 weeks which increased maximum oxygen uptake by about 10%. The data for delta(A-I) were used to compute the relative difference (deltaU) between the resistances of 16O18O and 16O2 for oxygen transport from the alveolar space and utilization in the mitochondria. Prior to training, deltaU decreased from 15 per thousand at rest to 5 per thousand at the highest level of exercise and after training from 12 to 5 per thousand. The difference between the results for deltaU before and after training was significant for rest (P < or = 5) but not for exercise conditions. Accordingly, we conclude that for exercise conditions the non-fractionating oxygen transport by blood flow to and the fractionating oxygen transport by diffusion in the muscles have improved by training to more or less the same degree. The decrease in deltaU in rest after training suggests that oxygen transport by diffusion in other tissues also benefits from the effects of training.