Methods of isolating intact thrombocytes from blood]

A combined method of platelet isolation in albumin density gradient followed by gel filtration through a column packed with Sepharose 2B appears to be the best for the isolation of intact platelets free from plasma proteins, fit for research purposes; for clinical studies a more available technique for intact platelet isolation in albumin density gradient is recommended.     

周围型肺肿块周边血流的彩超研究

目的利用彩色多普勒血流显像(CDFI)和脉冲波多普勒技术(PW),对周围型肺肿块周边血流进行研究,以鉴别肿块的良恶性.方法对51例周围型肺肿块(良性18例,恶性33例)周边血流进行CDFI和PW检查.结果良恶性肺肿块周边血流的显示率分别为33%和88%,两者间有显著差异.恶性肿瘤血流程度多为Ⅱ～Ⅲ级,而良性肿瘤多为0～Ⅰ级,两者间有显著差异.恶性肿瘤RI0.47±0.06,PI1.18±0.16,PSV 0.17±0.04m/s,EDV0.07±0.02m/s;良性肿瘤RI0.81±0.12,PI1.68±0.33,PSV 0.29±0.05m/s,EDV0.04±0.01m/s,两者在血流4项指标间有显著性差异.并以RI＜0.59,PSV≤0.25m/s,作为诊断肺恶性肿瘤的标准.结论CDFI和PW能显示肿块周边血流的血管分布和血流频谱,有助于鉴别其良恶性.     

Minimal expression of blood group A antigen on thrombocytes from A2 individuals

Two multiply transfused group O patients developed refractoriness to random-donor platelet transfusions. Both had high-titer IgG antibodies to group A and B antigens. Subsequent transfusions with ABO-compatible platelets were successful, suggesting that the ABO blood group system is significant in the development of refractoriness to platelet transfusions. By indirect immunofluorescence, the patients' IgG anti-A reacted with platelets from group A1 donors but not with those from group A2 donors. A2 platelets were at least 38 times less efficient at adsorbing a mouse monoclonal anti-A; the difference between the adsorbence capacities of A2 and O platelets was minimal. Transfusion of A2 platelets, unlike that of A1 platelets, effected a good increment in the platelet counts of these two patients. The results indicate that the expression of A2 antigen on platelets from A2 subjects is minimal.     

多发性硬化患者外周血活化淋巴细胞的检测及临床意义

目的 探讨多发性硬化(MS)患者外周血淋巴细胞数量变化及淋巴细胞的激活状态与临床意义。方法 采用流式细胞仪检测MS患者淋巴细胞CD3、CD4、CD8、CDl9、CDl6 56、CD25、CD69、HLA-DR。结果 MS患者外周血CD3、CD4、CDl6 56都显著低于正常对照组，CD4／CD8、CDl9较对照组高，复发期MS患者外周血CD25、HLA-DR、CD3／HLA-DR高于对照组和缓解期MS患者，缓解期MS患者CD3／HLA-DR高于对照组。结论 MS患者外周血淋巴细胞数量和质量上存在异常，并且淋巴细胞活化明显增高，与MS活动相关。     

外周血miRNA的诊断意义

micro RNAs是一类广泛存在于真核细胞中的长约22个核苷酸的非编码RNA分子。外周血miRNA与生长发育及妊娠等生理现象的改变密切相关,在疾病的诊断、鉴别诊断、预后判断以及法医鉴定等方面发挥着重要作用。     

吸烟人群24小时血压变化特征

目的:分析吸烟人群24h血压变化的特征。方法:选择2004-05/2004-10在盛京医院体检的健康沈阳市内五区居民482人,根据有无吸烟情况分组后再按相同性别、年龄、身高、体质量、职业1:1配对。共配成115对,吸烟组115人,不吸烟组115人。采用动态血压监测仪监测观察对象的24h及昼夜动态血压变化,日间(6:00～22:00)每15min测试1次,夜间(22:00～次日6:00)每30min测试1次,共记录80次。所采集数据经自动分析后,分别自动打印出24h、日间、上下午和夜间5个时段收缩压、舒张压和心率的平均值。血压昼夜节律判定采用张维忠的动态血压正常值协作研究所规定的标准,既夜间血压平均值比日间下降10%以上称之为杓型改变,夜间血压平均值比日间下降<10%称之为非杓型改变。aχ2=4.158,4.258,4.369,4.298,P<0.05。结果:按意向处理分析,进入结果分析115对,吸烟与不吸烟者分别为115人。①两组除上午平均收缩压、夜间平均收缩压、夜间平均舒张压有明显差异外(P>0.05),其余各时段各项血压指标均有明显差异(t=1.982～2.439,P<0.05)。②在血压昼夜节律形态分布比较中,吸烟组的平均收缩压杓型改变例数、平均舒张压杓型改变例数均明显低于不吸烟组犤33人(28.7%),39人(33.9%);70人(60.9%),78人(67.8%),χ2=4.158,4.369,P<0.05犦。结论:吸烟者白昼常有较高的平均收缩压、舒张压,同时血压昼夜节律中以非杓型改变居多。     

光动力作用对人外周血中性粒细胞免疫影响的研究

目的　探讨光动力作用对人类外周血中性粒细胞免疫功能的影响。方法　采用酵母菌粘附试验 ,观察光动力作用对正常人中性粒细胞的粘附功能的影响 ,以及在光动力作用下 ,正常人红细胞对中性粒细胞吞噬功能的促进作用。实验将正常人的外周血中性粒细胞分离后 ,短时间暴露于小剂量的血卟啉衍生物及红光下 ,通过光镜观察活化的中性粒细胞与致敏酵母多糖的免疫粘附。结果　小剂量光动力作用可以显著增强中性粒细胞的免疫粘附功能 ,其C3b受体花环率为 (2 2 .5 2±5 .79) % ,明显大于对照组 (14.33± 4.5 6 ) % (P <0 .0 1) ,同时可增强红细胞促进中性粒细胞的粘附吞噬功能。结论　小剂量光动力作用可以促进人类外周血中性粒细胞及红细胞表面C3b受体活性 ,增强其免疫粘附功能。     

Decreased quality of peripheral blood progenitors collected after a peripheral blood progenitor transplant

The purpose of this study was to determine the quality of peripheral blood progenitor cells (PBPC) collected after an initial autologous PBPC transplant. Tandem autologous transplants have been used in the treatment of several malignancies. Routinely, PBPC have been collected prior to the first transplant and used for both transplants. In the current study, PBPC harvested prior to the first high-dose therapy (HDT) were used as a source of progenitors for transplant 1, and a combination of bone marrow harvested prior to the first course of HDT and PBPC collected approximately 85 days after the first transplant were used to support the second HDT. We analyzed the quality of the PBPC collected 85-120 days after HDT and autologous PBPC transplant. CD34 and colony-forming units granulocyte-macrophage (CFU-GM) contents of those collections were poor, and hematopoietic recovery was more consistent with recovery from a bone marrow transplant than a PBPC transplant. Thirteen of 15 patients received both transplants. Days to absolute granulocyte count of 500 was 10 +/- 1.5 for the first transplant and 13.3 +/- 3.7 for the second (p < 0.01). The number of days to platelet count of 20,000 was 14.3 +/- 10.7 for the first transplant and 18 +/- 7 for the second transplant (p = 0.066). The number of days of total parenteral nutrition (TPN) and intravenous morphine used by patients for the first and second transplants was similar, whereas the length of hospitalization was 21.8 +/- 3.6 for the first transplant and 27.6 +/- 7.8 for the second transplant (NS). In conclusion, it appears that the quality of PBPC collected following a previous PBPC transplant may be compromised.     

脑梗塞患者外周血白细胞介素—6活性研究

目的：探讨白细胞介素６（ＩＬ６）在脑梗塞发病中的作用及临床意义。方法：采用３Ｈ胸腺嘧啶核苷掺入法检测３２例正常人及４０例脑梗塞患者外周血单核细胞ＩＬ６活性，并对其中１８例脑梗塞患者恢复期ＩＬ６活性进行了复查。结果：脑梗塞组急性期患者ＩＬ６活性〔（１３０．８８±１５．０２）ｋＵ／Ｌ〕显著高于正常对照组〔（９４．２３±１３．８１）ｋＵ／Ｌ〕，但低于恢复期〔（１１３．７５±１４．８９）ｋＵ／Ｌ〕，Ｐ均＜０．００５；ＩＬ６活性变化与脑梗塞容积密切相关，梗塞容积越大，ＩＬ６活性越高；脑梗塞患者恢复期ＩＬ６活性显著低于急性期，但仍显著高于正常对照组。结论：ＩＬ６在脑梗塞发生及发生后的病理变化上均起重要作用，检测ＩＬ６活性对推断脑梗塞容积的大小有一定意义。