研究网络资源类型及特点对做好用户参考咨询的作用

本文主要探讨网络参考资源的类型和特点，参考咨询服务的方式，网络资源使用方面存在的问题。就图书馆如何加强对用户的参考服务，从应提高认识转变观念，设立有特色咨询主页，构建咨询源保障体系，开展网上导航工作，加强咨询协作网建设，提高信息资源利用率，不断提高馆员素质等方面进行详细论述，以拓展图书馆在网络时代创新服务的模式。     

医院图书馆数字化建设实践初探

简单介绍了南方医院图书馆数字化建设的情况,提出医院图书馆数字化建设的几点体会:1、要制定切实可行的建设规划,2、要加强特色数据库建设,3、要加强虚拟参考咨询建设,4、要加强馆际协作与资源共享,5、要积极整合利用网络信息资源,6、要切实做好信息安全管理,7、要加强高素质人才队伍建设.     

浅谈本校图书馆网站的开发与利用

医学高校图书馆是教学、科研与临床的信息中心,为它们提供准确快捷的信息保障.进入信息化时代后,各高校图书馆也纷纷由传统的手工服务阶段步入了通过计算机网络的自动化服务阶段.随之发展的高校图书馆网站也日趋成熟稳定.如何建成一个服务目标明确,栏目设置合理,方便用户使用的既实用又美观的网站,以更好的质量服务更多的用户.是每个高校图书馆追求的目标.图书馆网站是图书馆提供信息服务的窗口,图书馆网站应通过开展各项针对读者的服务项目.使读者足不出户就可以享受到图书馆的各项优质服务,这是图书馆网站能够吸引人的魅力所在.笔者浏览了国内很多高校图书馆的网站.认为栏目的 设置大体都有本馆概况、动态公告、服务指南、数据库资源、自建特色资源、参考咨询(课题查新、馆际互借、文献传递、实时解答、BBS、留言本)、网络导航等几大块(名称不尽相同),但又各有特色.版面的设计更是不拘形式.本文旨在通过本校图书馆网站建设的实例.归纳特色,指出不足,并提出改进建议.     

了解网络资源类型特点 加强用户参考咨询服务

本文主要探讨网络参考资源的类型和特点,参考咨询服务的方式,网络资源使用方面存在的问题。就图书馆如何加强对用户的参考服务,从应提高认识转变观念,设立有特色咨询主页,构建咨询源保障体系,开展网上导航工作,加强咨询协作网建设,提高信息资源利用率,不断提高馆员素质等方面进行详细论述,以拓展图书馆在网络时代创新服务的模式。     

未来信息天使——参考咨询馆员

阐述了未来图书馆参考咨询馆员的工作特点，继而论述了现代图书馆信息服务所具有的一些特征，提出了现代图书馆应加强参考咨询馆员信息服务能力的培养。     

开发期刊信息资源为高校重点学科建设服务

本文论述了高校图书馆合理配置期刊资源的原则,开发期刊信息资源的方法,指出深化期刊信息服务,对重点学科建设有着十分重要的意义。     

高校图书馆馆员如何做好咨询工作

本文分析了高校图书馆咨询工作的提出以及高校图书馆馆员要做好咨询工作必须要具备多方面的素质和咨询服务的新理念。     

新世纪高校图书馆信息服务之展望--试论高校图书馆信息服务

21世纪是知识经济时代，知识、信息就是财富，就是资源，而高校图书馆正是知识．信息的重要来源，图书馆必须强化信息服务，以适应时代的要求。本文欲从信息开发、信息检索、信息咨询三个方面展开论述。     

信息化技术对高校图书馆工作的影响

随着现代信息技术在图书馆中的广泛应用，我国高校图书馆信息化建设得到了迅速发展，图书馆已成为校园信息网中信息资源的重要枢纽。本文将重点从信息化技术对图书馆的管理、服务和馆员三方面论述信息化技术读高校图书馆工作的影响。     

普通高校图书馆实施学科馆员制度初探

学科馆员制度是高校图书馆深化信息服务的一种新形式,国内许多重点院校图书馆已相继推出此项服务,本文结合本馆实践,探讨了普通高校图书馆开展学科馆员工作的实施策略。     

热带医学专业信息资源库几个重要问题的思考及其改进

基于校园网和广东省专业信息资源库平台，构建“热带医学专业信息资源库”。对试题库、动画库、专家库、powerpoint教学资源库等几个重要问题进行思考，提出改进的建议。     

即时通讯在图书馆实时参考咨询工作中的应用

即时通讯软件是融合了多种信息技术的Internet集大成者,图书馆利用其开展实时参考咨询具有现实性与可行性。即时通讯软件将图书馆、计算机系统、数据库、网络和读者融为一体,为图书馆的实时参考咨询插上飞翔的翅膀。     

Identification of Nocardia species from clinical isolates using MALDI-TOF mass spectrometry

The identification of Nocardia isolates still represents a challenge for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) despite its acceptance for most bacterial and fungal isolates. In this study we evaluate the identification of Nocardia isolates using direct spotting and an updated database.Overall, 82 Nocardia isolates belonging to 13 species were identified by DNA sequence analysis of the 16S rRNA and secA1 genes. Nine of these well-characterized isolates from 6 Nocardia species were used to create an in-house library. The remaining 73 isolates were directly spotted on the target plate and on-plate protein extraction was performed. The protein spectra obtained were analyzed by MALDI-TOF MS using the BDAL database (Bruker Daltonics) updated with 6,903 MSPs or the combination of this commercial database and our in-house library.As a result, the use of the commercial database alone and in combination with the in-house library yielded 94.5% and 95.9% of correct species-level identifications, respectively, No isolate was misidentified at the genus level with either database. Besides, the use of the in-house library allowed the species-level identification of a N. otitidiscaviarum isolate that could only be identified at the genus-level with a score value <1.6 using the commercial database.In conclusion, the implementation of the direct spotting method and the in-house database provided a high rate of correct species assignment of Nocardia isolates despite the low number of isolates added. Further addition of well-characterized Nocardia isolates may ensure the rapid, accurate and inexpensive identification of most isolates encountered in the routine of the microbiology laboratory.     

Characterisation of the putative nucleoprotein gene of infectious salmon anaemia virus (ISAV)

A gene encoding the putative nucleoprotein (NP) of infectious salmon anaemia virus (ISAV), a commercially important salmonid Orthomyxovirus, has been identified. cDNA obtained from a subtractive cDNA library bound specifically to RNA extracted from ISAV-infected SHK-1 cell cultures. The 5' and 3' ends of the gene were amplified using RACE PCR and a full length open reading frame (ORF) of 1851 nt identified encoding a predicted protein of 616 amino acids. No significant homology of this sequence with any other orthomyxovirus nucleoprotein was identifiable using BLAST or FASTA-based database searches. The ISAV-protein was however identified as a nucleoprotein based on its characteristic amino-acid composition. Furthermore the conserved sequence 5' GCAAAGA 3' was identified preceding the ORF, as has been identified in all other ISAV-genes characterised to date.     

CpG Islands of the Pig

We describe an analysis of the CpG islands (CGIs) of the pig. We have used both database survey and a porcine genomic library that is enriched for CGIs. Approximately half of 41 pig genomic database sequences had CGIs with an average G+C content of 65.3%, an average CpG observed/expected frequency of 0.85, and an average size of 978 bp. Of 27 CGI library clones, 16 were nonrepetitive, nonribosomal DNA and CGI-like. CGI library clones had similar average values for G+C and CpG frequency to CGIs of database genes, and an average size of 670 bp, as MseI cuts within some islands. Library clones were also shown to be low copy number and unmethylated in genomic DNA. The presence in the library of seven previously known CGI sequences was confirmed as was the absence of one nonisland sequence. The CGI library exhibits an R-band pattern for many chromosomes in FISH analysis. The pig chromosome arms that show the most dense CGI population are homologous to segments of human chromosomes that are known to be gene rich.     

Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library

BACKGROUND: Intracellular localization is an important part of the characterization of a gene product. In an attempt to search for genes based on the intracellular localization of their products, we constructed a green fluorescent protein (GFP)-fusion genomic DNA library of S. pombe. RESULTS: We constructed the S. pombe GFP-fusion genomic DNA library by fusing, in all three reading frames, random fragments of genomic DNA to the 5' end of the GFP gene in such a way that expression of potential GFP-fusion proteins would be under the control of the own promoters contained in the genomic DNA fragments. Fission yeast cells were transformed with this plasmid library, and microscopic screening of 49 845 transformants yielded 6954 transformants which exhibited GFP fluorescence, of which 728 transformants showed fluorescence localized to distinct intracellular structures such as the nucleus, the nuclear membrane, and cytoskeletal structures. Plasmids were isolated from 516 of these transformants, and a determination of their DNA sequences identified 250 independent genes. The intracellular localizations of the 250 GFP-fusion constructs was categorized as an image database; using this database, DNA sequences can be searched for based on the localizations of their products. CONCLUSIONS: A number of new intracellular structural components were found in this library. The library of GFP-fusion constructs also provides useful fluorescent markers for various intracellular structures and cellular activities, which can be readily used for microscopic observation in living cells.     

抗恶性疟SERA噬菌体抗体库的构建及单链抗体的筛选与鉴定

目的 研制用于疟疾简便,快速诊断试剂盒的试剂。方法 利用噬菌体抗体库技术,构建抗恶性疟原虫丝氨酸重复抗原（SERA）的噬菌体抗体库。经3轮“吸附－洗脱－扩增”的富集反应后,从中筛选抗SERA的阳性克隆株,并进行可溶性诱导表达,最后用ELISA和Western blot等进行鉴定。结果 成功地构建了中等库容的噬菌体抗体库,并从中筛选到9株抗SERA的阳性克隆株,表达的单链抗体的相对分子质量大小约为31kDa,能与SE47‘抗原起特异性结合反应。结论 成功地构建了抗SE47‘的噬菌体抗体库,从中筛选制备的单链抗体为研制疟疾快速诊断试剂盒奠定了基础。     

中国医院知识仓库(CHKD)在医院信息保障中的作用

本文重点介绍了《中国医院知识仓库(CHKD)》数字期刊数据库的特点及在医院图书馆的应用，CHKD的应用对加强医院信息资源建设，对医院学术研究、医疗服务质量提高的推动作用，为医院图书馆向网络化、数字化、虚拟化发展提供了一个良好的契机，也为未来医院图书馆的数字化发展指明了方向。     

Computer-based methods for the mouse full-length cDNA encyclopedia: real-time sequence clustering for construction of a nonredundant cDNA library

We developed computer-based methods for constructing a nonredundant mouse full-length cDNA library. Our cDNA library construction process comprises assessment of library quality, sequencing the 3' ends of inserts and clustering, and completing a re-array to generate a nonredundant library from a redundant one. After the cDNA libraries are generated, we sequence the 5' ends of the inserts to check the quality of the library; then we determine the sequencing priority of each library. Selected libraries undergo large-scale sequencing of the 3' ends of the inserts and clustering of the tag sequences. After clustering, the nonredundant library is constructed from the original libraries, which have redundant clones. All libraries, plates, clones, sequences, and clusters are uniquely identified, and all information is saved in the database according to this identifier. At press time, our system has been in place for the past two years; we have clustered 939,725 3' end sequences into 127,385 groups from 227 cDNA libraries/sublibraries (see http://genome.gse.riken.go.jp/).