原发性乳房外Paget病中雄激素受体的表达

目的探讨雄激素受体(AR)在原发性乳房外Paget病组织中的表达及其与疾病侵袭性间的关系。方法应用免疫组化方法,检测20例原位乳房外Paget病、18例侵袭性乳房外Paget病石蜡组织切片中AR的表达情况。结果 38例原发性乳房外Paget病中男26例,女12例,年龄(45~84)岁,平均年龄为64岁。AR在原发性乳房外Paget病中明显表达,AR高表达率为58%(22/38);其中,AR在侵袭性及原位乳房外Paget病中的高表达率分别为78%(14/18)和40%(8/20),相比具有统计学意义(χ2值为5.401,P值为0.020)。结论 AR的异常表达与原发性乳房外Paget病的发生发展及侵袭性可能有关。     

Pin1在乳房外Paget病的表达及意义

目的：分析Pin1染色后乳房外Paget病组和对照组切片中Pin1的表达及意义。方法：采用免疫组化染色的方法,对27例乳房外Paget病和10例正常皮肤标本进行Pin1免疫组化染色,并将乳房外Paget病组按照浸润深度分成三组分别观察。结果：乳房外Paget病组阳性率74．07％,其中51．85％为高表达,对照组阳性率30％,均为低表达,差异具有统计学意义（P〈0．05）。乳房外Paget病标本按照浸润深度分成三组进行比较,差异来自对照组与乳房外Paget病各组之间,在乳房外Paget病组内部进行两两比较无统计学意义。结论：Pin1在正常皮肤组织中不表达或极低表达,在乳房外Paget病患者皮损中的表达增高。     

原发性乳房外Paget病中E钙黏蛋白的检测

目的观察乳房外Paget病中E钙黏蛋白的表达并分析其在肿瘤侵袭过程中的意义。方法应用免疫组化方法分别对28例原发性原位乳房外Paget病(A组)和17例原发性侵袭性乳房外Paget病(B组)标本中的E钙黏蛋白进行检测。结果原发性原位乳房外Paget病标本中E钙黏蛋白的表达较周边正常皮肤标本中表达下调,其中28例原发性原位乳房外Paget病标本中,E钙黏蛋白阴性表达的占28.6%(8/28),低表达的占46.4%(13/28),高表达的占25.0%(7/28),17例原发性侵袭性乳房外Paget病标本表达分别为17.6%(3/17),58.8%(10/17),23.6%(4/17),9例肿瘤边缘正常皮肤和2例正常阴囊皮肤中E钙黏蛋白全部高表达,正常皮肤组与两组乳房外Paget病中E钙黏蛋白的表达差异有显著性(P<0.01),而两组乳房外Paget病之间的表达差异无显著性(P>0.05)。结论E钙黏蛋白表达的下调与原发性乳房外Paget病的发病有关,但可能与侵袭过程没有必然的联系。     

P16和PCNA在乳房外Paget病的检测及相关性分析

目的 探讨P16和PCNA在乳房外Paget病中的表达特点及二者的关系.方法 应用免疫组化二步法检测34例乳房外Paget病及20例正常对照皮肤组织中P16和PCNA的表达.结果 乳房外Paget病组织中P16阳性率为29.4%,正常皮肤组织未见表达,两者差异有显著性(P<0.05).PCNA在乳房外Paget病组织中阳性率为85.29%,在正常皮肤组织为55.00%,两者差异有显著性(P<0.05).有淋巴结转移组中P16,PCNA的阳性表达均高于无淋巴结转移组,但P16的表达差异无显著性(P＞0.05).结论 P16和PCNA可能参与了乳房外Paget病的发病过程,并且PCNA与乳房外Paget病的淋巴结转移有关.     

乳房Paget病肿瘤组织中Bcl－2、Bax、雌激素和孕激素受体表达的研究

表1 Bcl－2、Bax、雌激素受体和孕激素受体在乳房Paget病中的表达 乳房 Paget病 (MPD)是一种特殊类型的皮肤肿瘤，患者往往合并下方乳腺 癌 [1]。近年来，乳腺肿瘤组织中癌基因、抑癌基因以及凋亡相关基因研究报道日益增多，揭示乳腺癌的发生发展涉及到上述基因之间的相互作用 [2]。我们采用 SP免疫组化方法，观察 Bcl－ 2、 Bax在 MPD及其下方乳腺癌中的表达，并与雌激素受体（ ER）、孕激素受体 （ PR）的表达情况进行比较，旨在探讨凋亡相关基因与 MPD的发病关系以及 MPD与下方乳腺癌之间的关系。 一、 材料与方法 1.标本：…     

原发性乳房外Paget病组织中钠氢交换子调节因子1和β联蛋白的表达和意义

【摘要】 目的 探讨钠氢交换子调节因子1（NHERF1）和β联蛋白在原发性乳房外Paget病组织中的表达及其意义。 方法 应用免疫组化方法,检测18例原位乳房外Paget病、22例侵袭性乳房外Paget病石蜡组织切片中NHERF1和β联蛋白的表达情况。 结果 NHERF1在22例侵袭性乳房外Paget病中18例（81.82%）为高表达,18例原位乳房外Paget病中7例（38.89%）高表达,两组差异有统计学意义（χ2 = 7.78,P ＜ 0.01）;β联蛋白在侵袭性乳房外Paget病中有0例胞膜高表达和18例（81.82%）胞质或核高表达,原位乳房外Paget病中分别为6例（33.33%）和8例（44.44%）,两组差异均有统计学意义（χ2值分别为8.63和6.08,P值分别 ＜ 0.01和 ＜ 0.05）。原发性乳房外Paget病组织中NHERF1的表达与β联蛋白胞膜表达呈负相关（ρ = -0.488,P ＜ 0.01）,与β联蛋白胞质或核表达呈正相关（ρ = 0.623,P ＜ 0.01）;β联蛋白胞膜表达与胞质或核表达呈负相关（ρ = -0.572,P ＜ 0.01）。 结论 乳房外Paget病组织中NHERF1和β联蛋白表达异常,并可能与原发性乳房外Paget病的发生发展及侵袭有关。 【关键词】 佩吉特病,乳腺外; β连环素; 钠氢交换子调节因子1     

PTEN和Ki67在乳房外Paget病中的表达

目的观察PTEN和Ki67在乳房外Paget病中的表达特点及其临床意义。方法应用免疫组化二步法分别检测30例乳房外Paget病患者及20例正常人皮肤组织中PTEN和Ki67的表达情况。结果PTEN在乳房外Paget病中阳性表达为66.67%,显著低于正常皮肤组织的阳性表达(100.00%,P0.05)。Ki67在乳房外Paget病中阳性表达为83.33%,显著高于正常皮肤组织中的阳性表达(50.00%,P0.05)。乳房外Paget病中PTEN表达和Ki67表达呈负相关关系(P0.05)。结论PTEN的低表达和Ki67的过表达在乳房外Paget病的发生和发展中可能起着十分重要的作用。     

CK5/6和CK14在乳房外Paget病的分布

目的观察细胞角蛋白5/6(CK5/6)及14(CK14)在乳房外Paget病中的表达特点,并探讨其生物学意义。方法应用免疫组化二步法分别检测34例乳房外Paget病患者皮损及20例正常对照皮肤中CK5/6及CK14的表达情况。结果 CK5/6和CK14在乳房外Paget病中阳性表达,两者主要在表皮全层弥漫性表达,表达率分别为91.17%和88.24%。结论 CK5/6和CK14在乳房外Paget病的组织来源方面起到一定的启示作用,推测乳房外Paget病的肿瘤细胞可能来源于异常分化的基底细胞层表皮干细胞。     

汗管瘤雌孕激素受体表达的研究

目的探讨性激素在汗管瘤发生中的作用.方法应用免疫组化法检测了13例汗管瘤雌激素及孕激素受体表达.结果 12例汗管瘤细胞表达孕激素受体,13例汗管瘤细胞均无雌激素受体表达.结论汗管瘤的发生可能与孕激素有关.     

Cyclin D1、PCNA和C-erbB-2在乳房外Paget病中的表达及意义

目的研究c7clin D1、PCNA和C—erbB-2在乳房外Paget病中的表达及意义。方法用免疫组织化学技术检测28例乳房外Paget病的石蜡包埋组织及12例正常皮肤组织中Cyclin D1、PCNA和C—erbB-2的表达。结果CyclinDI在乳厉外Paget病组织中阳性率（92．86％）高于正常皮肤对照组（8．33％）,两者差异有统计学意义（P〈0．05）;PCNA在乳房外Paget病组织中阳性率（89．29％）显著高于正常皮肤对照组（16．67％）,两者差异有统计学意义（P〈0．01）;C—erbB-2在乳房外Paget病组织中阳性率（96．43％）高于正常皮肤对照组（8．33％）,两者差异有统计学意义（P〈0．05）。结论Cyclin D1、PCNA和C-erbB-2在乳房外Paget病组织中均呈高表达,其与乳房外Paget病的发生关系密切。     

宫腔镜联合左炔诺孕酮宫内缓释系统治疗子宫内膜息肉临床观察

目的:探讨左炔诺孕酮宫内缓释系统联合子宫内膜息肉切除术对雌、孕激素受体及表皮生长因子的影响。方法:选择2013年1月至2015年1月收治的子宫内膜息肉患者120例为研究对象,根据入院时间按照单双号随机分为观察组60例与对照组60例,所有患者均接受子宫内膜息肉切除术,观察组术后联合左炔诺孕酮宫内缓释系统治疗,对照组术后口服复方短效避孕药(屈螺酮3mg+炔雌醇30μg)。比较临床症状、雌孕激素受体与表皮生长因子表达水平、复发率、不良反应等指标。结果:随访12个月,观察组月经失血图(PBAC)评分、子宫内膜厚度均明显低于对照组,血红蛋白明显高于对照组(t=14.390、14.037、7.659,P<0.05);雌激素受体(ER)、孕激素受体(PR)、表皮生长因子(EGFR)表达均明显低于对照组(t=6.721、8.420、7.496,P<0.05);复发率、不良反应等均明显低于对照组(1.67%vs.11.67%,6.67%vs.25.00%)(χ~2=4.821、7.566,P<0.05)。结论:左炔诺孕酮宫内缓释系统联合子宫内膜息肉切除术有助于减少子宫内膜息肉患者的雌、孕激素受体及表皮生长因子表达,改善临床症状,预防术后复发,降低不良反应。     

颧部褐青色痣与性激素相关性的研究

目的:探讨颧部褐青色痣（NFZ）患者血清性激素水平及皮损性激素受体与NFZ发病的相关性。方法: 采用免疫组化法检测雌激素受体（ER）、孕激素受体（PR）及雄激素受体（AR）在10例NFZ及蓝痣石蜡标本上的表达,同时对30例女性NFZ患者采用放射免疫法检测血清性激素（FSH 、P、 E2 、T、LH 及PRL）的水平,并将其结果与健康女性血清性激素水平加以比较。结果:NFZ皮损真皮黑素细胞免疫组化染色有5例AR阳性,ER和PR均为阴性,NFZ患者与健康人比较,血清E2、P、T、FSH、LH、PRL差异无显著性（p>0.05）。结论:NFZ的发病与性激素E2、P、T、FSH、LH、PRL水平无关,但其黑素细胞有雄激素受体。     

Spa-1和Ki-67在乳房外Paget病肿瘤组织中的表达

目的 探讨Spa-1在乳房外Paget病发生和转移中可能的作用.方法 取17例原位乳房外Paget病和12例侵袭性乳房外Paget病患者皮损及9例正常人皮肤组织,用免疫组化分别检测Spa-1和Ki-67的表达.结果 Spa-1在胞质中显色,Ki-67在胞核中显色.Spa-1和Ki-67仅少量表达于正常人皮肤基底层,在乳房外Paget病肿瘤组织中的表达明显高于正常人皮肤(P<0.01).Spa-1在侵袭性乳房外Paget病肿瘤组织中表达明显高于原位乳房外Paget病(P<0.01).乳房外Paget病肿瘤组织中Spa-1的表达与Ki-67的表达呈正相关.结论 Spa-1在乳房外Paget病的发生发展中可能起作用.

Abstract：

Objective To investigate the potential role of Spa-1 in the development and metastasis of EMPD.Methods Tissue specimens were resected from 17 patients with primary EMPD,12 patients with invasive EMPD and 9 normal human controls.Immunohistochemistry was performed to measure the expression of spa-1 and Ki-67.Results Positive staining was observed for Spa-1 in cytoplasm,and for Ki-67 in cell nuclei.Spa-1 and Ki-67 were weakly expressed in the basal layer of normal skin.The expression of Spa-1 and Ki-67 in EMPD tissue were statistically higher than those in the normal control tissue (both P<0.01).Increased expression of Spa-1 was noted in invasive EMPD tissue compared with in situ EMPD tissue.The expression of Spa-1 was positively correlated with that of Ki-67 in the tissue of EMPD.Conclusion Spa-1 may play a certain role in the initiation and progression of EMPD.     

Expression of the p38 MAPK, NF-kappaB and cyclin D1 in extramammary Paget's disease

BACKGROUND: The p38 mitogen-activated protein kinase (MAPK)/nuclear factor kappaB (NF-kappaB)/cyclin D1 signaling pathway has recently been shown to play an important part in the pathogenesis of many human tumors. However, the role of this signal transduction pathway in extramammary Paget's disease (EMPD) remains unknown. OBJECTIVE: This study was designed to investigate the expression of phosphorylated p38 MAP kinasealpha (p-p38 MAPKalpha), phosphorylated NF-kappa B p65 (p-NF-kappaB p65) and cyclin D1 proteins in EMPD and to evaluate the relationship among them. METHODS: Thirty-five tissue samples from 30 primary EMPD cases were analyzed by immunohistochemical staining in formalin-fixed, paraffin-embedded tissue sections for p-p38 MAPKalpha, p-NF-kappaB p65 and cyclin D1. RESULTS: Among the 35 specimens of EMPD, p-p38 MAPKalpha, p-NF-kappaB p65 and cyclin D1 were expressed in 30, 28 and 27, respectively. Moreover, in five metastatic lymph node specimens, all were positive for p-p38 MAPKalpha and p-NF-kappaB p65, four were positive for cyclin D1. There were significant correlations between expression of p-p38 MAPKalpha, p-NF-kappaB p65, and cyclin D1 in EMPD. CONCLUSION: This study provides evidence that p-p38 MAPKalpha, p-NF-kappaB p65, and cyclin D1 was overexpressed in EMPD, suggesting that the p38 MAPK/NF-kappaB/cyclin D1 signaling pathway might participate in the oncogenesis of EMPD.     

Expression and prognostic significance of Stat5a and E-cadherin in extramammary Paget's disease

BACKGROUND: Stat5 has been shown to regulate the proliferation and inhibition of apoptosis in cancer cells. E-cadherin plays an important role in maintaining epithelial stability and is widely regarded as a prognostic marker in many types of human cancers. The expression of Stat5 has not been investigated in extramammary Paget's disease (EMPD). OBJECTIVES: To study the expression of Stat5a and E-cadherin protein in EMPD and to evaluate the relationships between them. METHODS: Thirty-six tissue samples from 34 cases with primary EMPD were subjected to immunohistochemical staining for Stat5a and E-cadherin. RESULTS: Twenty-nine of 30 in situ EMPD specimens were positive for Stat5a expression, and the staining pattern for Stat5a was mainly nuclear in the majority of Paget's cells. All four invasive specimens and two metastatic lymph node specimens were negative for Stat5a. Twenty-six of 30 in situ EMPD specimens expressed E-cadherin in more than 50% of tumor cells, and three of 30 in situ EMPD specimens expressed E-cadherin in less than 50% of tumor cells. One in situ EMPD specimen and one invasive specimen expressed E-cadherin in less than 25% of tumor cells. Three invasive specimens and two metastatic lymph node specimens were negative, with occasional membranous staining for E-cadherin. There is a significant correlation between the expression of Stat5a and E-cadherin. CONCLUSIONS: Our results showed that these proteins were found in in situ lesions, and it is speculated that these proteins may play some role in the prognosis/invasion of EMPD.     

乳房外Paget病皮损中黏蛋白1和黏蛋白2的表达

目的 探讨黏蛋白1(MUC1)和黏蛋白2(MUC2)在乳房外Paget病(EMPD)中的表达情况.方法 生物素蛋白免疫组化法(SP法)检测19例EMPD皮损及19例美容切除术后正常皮肤组织上MUC1与MUC2的表达.结果 19例EMPD皮损常规HE染色显示,3例伴低分化腺癌,6例呈浸润性,10例为上皮内.MUC1在3例伴腺癌Paget病中有2例呈阳性表达,6例浸润性和10例上皮内Paget病均呈阳性表达.MUC2在3例伴腺癌Paget病和6例浸润性Paget病均呈阳性表达,在10例上皮内Paget病中有2例呈阳性表达.MUC1与MUC2在正常皮肤组织呈阴性表达.MUC1在上皮内Paget病中的表达显著高于伴腺癌Paget病和浸润性Paget病(P＜0.05).MUC2在伴腺癌Paget病和浸润性Paget病中的表达显著高于上皮内Paget病(P＜0.05).MUC1和MUC2的表达无明显相关性(r=-0.5,P＞0.05).结论 MUC1在EMPD中呈普遍表达,MUC2在伴有腺癌和浸润性EMPD中呈阳性表达.

Abstract：

Objective To study the expressions of mucin (MUC) 1 and 2 in extramammary Paget's disease(EMPD) lesions. Methods Tissue specimens were obtained from the lesions of 19 patients with EMPD and normal skin of 19 human controls during cosmetic surgery. Streptavidin-perosidase (SP) technique was used to detect the expressions of MUC1 and MUC2 in these specimens. Results As haematoxylin-eosin (HE) staining showed, 3 cases were accompanied by poorly differentiated adenocarcinoma, 6 were invasive Paget's disease and 10 were intraepithelial EMPD. MUC1 was expressed in 2 cases accompanied by poorly differentiated adenocarcinoma, and in all the cases of invasive and intraepithelial EMPD; MUC2 was observed in all the cases of adenocarcinoma-complicated EMPD and invasive EMPD, but only in 2 of 10 cases of intraepithelial EMPD.Neither MUC1 nor MUC2 was observed in normal control specimens. A significant increase was observed in the expression of MUC1 in lesions of intraepithelial EMPD compared with invasive EMPD and adenocarcinoma-complicated EMPD (both P ＜ 0.05), and in the expression of MUC2 in lesions of invasive EMPD and adenocarcinoma-complicated EMPD compared with intraepithelial EMPD (both P ＜ 0.05). The expression of MUC1 was uncorrelated to that of MUC2 (r= -0.5, P＞ 0.05). Conclusions MUC1 is generally expressed in the lesions of EMPD, while MUC2 is expressed in those of adenocarcinoma-complicated EMPD and invasive EMPD.     

Expression of estrogen,androgen, and glucocorticoid receptors in recent striae distensae

Background Stretch marks or striae distensae (SD) can be considered a common skin disorder, but their physiopathogenic mechanisms have not been totally clarified. Although it is considered an esthetic complaint, it may have serious psychosocial consequences besides the local and systemic alterations of the conjunctive tissue. This study aims at assessing and quantifying the estrogen, androgen and glucocorticoid receptors in skin samples with striae and comparing with normal skin. Methods Skin samples for biopsy were obtained from eight patients with SD and eight patients without lesions. The samples were frozen at ?80 °C and underwent processing to obtain proteic extract to quantify the estrogen, androgen and glucocorticoid receptors with the Western Blot method. Results When the estrogen receptor in the skin with SD was compared with healthy skin, it was observed to have increased twice as much (P = 0.00001). The androgen and glucocorticoid receptors in the SD skin had also increased (P = 0.00015 and P = 0.00083, respectively). Conclusions These findings indicate that under certain conditions there is an increase in hormonal receptor expression, suggesting that regions that undergo greater mechanical stretching of the skin may express greater hormonal receptor activity. This activity may influence the metabolism of the extracellular matrix, causing the formation of SD. Alterations in hormone receptors occur within a well‐defined time period during the formation of SD; however, there are differences in the functionality of hormone receptors during different stages in the development of the lesions. The preliminary results appear to be relevant and represent aninitial step towards an understanding of the pathophysiology of SD.