竹节人参提取物抗实验性大鼠胃溃疡作用的实验研究

目的:观察竹节人参提取物对大鼠实验性胃溃疡的保护作用。方法:采用大鼠实验动物,造成大鼠幽门结扎型、无水乙醇型、冷应激型和消炎痛型溃疡模型,观察竹节人参提取物2g/kg、1g/kg 剂量下,对大鼠幽门结扎型、无水乙醇型、冷应激型和消炎痛型溃疡的作用,并结合与渍疡发病密切相关的胃蛋白酶活性、胃酸、胃溃疡发生面积等因素,检测出竹节人参提取物对它们的影响。结果:竹节人参提取物2g/kg、1g/kg 剂量下能明显控制大鼠幽门结扎型、无水乙醇型、冷应激型和消炎痛型溃疡的形成(P<0.01),减少溃疡形成面积(P<0.01)。结论:竹节人参提取物对治疗大鼠实验性胃溃疡具有良好的疗效,初步阐明了其抗溃疡的基本药理作用。     

树舌多糖抗溃疡作用

目的 :研究树舌多糖的抗溃疡作用。方法 :采用束缚 -应激模型、醋酸致胃溃疡模型、药物诱发模型和幽门结扎法研究树舌多糖抗胃溃疡作用。结果 :树舌多糖 2 0 0～ 10 0 0mg/kg可明显对抗应激型、醋酸型、药物 (消炎痛 )诱发型和幽门结扎型胃溃疡 ,对胃酸和胃蛋白酶活性皆有抑制作用。结论 :树舌多糖对大鼠实验性胃溃疡有明显保护作用。     

干地黄煎剂防止无水乙醇性胃粘膜损伤作用及其机制

目的:研究干地黄对胃粘膜的保护作用及机制。方法:给大鼠胃饲无水乙醇1ml 造模,观察干地黄对胃粘膜损伤的影响;采用不同途径给药和预先肌肉注射消炎痛进行分析。结果:(1)分别提前15、30和120min 胃饲干地黄煎剂6g/kg 均能显著抑制胃粘膜损伤;(2)分别胃饲3、6和12g/kg 的干地黄煎剂能不同程度地抑制胃粘膜损伤,且有一定的量效对应关系;(3)十二指肠内注射干地黄煎剂6g/kg 对胃粘膜亦有保护作用;(4)预先注射消炎痛5mg/kg 不能取消干地黄对胃粘膜损伤的预防作用。结论:干地黄对胃粘膜有明显的保护作用,其对无水乙醇性损伤的预防机理可能与胃腔内的理化作用及前列腺素合成无关。     

康尔胃冲剂抗大鼠实验性胃溃疡作用的实验研究

目的：探讨康尔胃冲剂治疗消化性溃疡的作用机理。方法：观察康尔胃冲剂对盐酸乙醇、消炎痛致大鼠急性胃粘膜损伤的作用，对水浸束缚法所致大鼠应激性溃疡的作用，对醋酸致大鼠胃小弯溃疡的作用及幽门结扎大鼠胃酸分泌的影响。结果：康尔胃冲剂可有效防治盐酸乙醇和消炎痛所致大鼠胃粘膜的损伤．对抗大鼠的应激性溃疡和醋酸所致慢性胃渍疡，并可抑制幽门结扎大鼠胃酸的分泌。结论：康尔胃冲荆具有抗溃疡、保护胃粘膜、抑制胃酸分泌等药理作用。     

胃顺舒片抗溃疡作用的实验研究

目的研究胃顺舒片的抗胃溃疡作用,并初步探讨其作用机制。方法采用小鼠水浸应激型胃溃疡、大鼠幽门结扎型胃溃疡2种实验性胃溃疡模型进行实验,观察胃顺舒片对实验性胃溃疡的抗溃疡作用。分别检测水浸应激型胃溃疡小鼠和幽门结扎型胃溃疡大鼠的溃疡指数,并测定幽门结扎型胃溃疡大鼠的胃酸和胃蛋白酶活性,以评价胃顺舒片的抗胃溃疡效果并初步探讨其作用机制。结果胃顺舒片可显著降低水浸应激型胃溃疡小鼠和幽门结扎型胃溃疡大鼠的溃疡指数,并能抑制幽门结扎型胃溃疡大鼠的胃酸和胃蛋白酶活性。结论胃顺舒片有明显的抗胃溃疡作用,其作用机制可能与其抑制胃酸和胃蛋白酶活性及抗应激有关。     

一贯煎对四种实验性胃溃疡的防治作用

以一贯煎煎剂10g/kg的剂量给动物灌胃,对四种实验性胃溃疡(慢性醋酸型、消炎痛型、利血平型和幽门结扎型)有明显的保护作用。研究表明一贯煎在不影响胃液分泌的情况下,可能通过多种机制发挥抗溃疡的作用。     

关苍术复方颗粒对胃溃疡大鼠的影响

目的:根据中药新药研究指南要求,研究关苍术复方颗粒的主要抗溃疡作用,为其申报临床研究提供依据。方法:采用大鼠应激型胃溃疡、幽门结扎型胃溃疡、无水乙醇型胃溃疡、消炎痛型胃溃疡四种模型进行实验。结果:关苍术复方颗粒(4.5g生药/g)5、2.5、1.25g/kg对大鼠应激型胃溃疡、幽门结扎型胃溃疡、无水乙醇型胃溃疡、消炎痛型胃溃疡均有明显的预防和治疗作用,且具有一定的量效关系。结论:关苍术复方颗粒对大鼠胃溃疡有明显的预防和治疗作用。     

山姜抗溃疡的实验研究

山姜水煎剂对幽门结扎型、应激型及利血平型大鼠实验性胃溃疡均有不同程度的抑制作用。但对消炎痛型胃溃疡作用不明显。它能增加胃液及胃蛋白酶活性，降低总胃酸与游离盐酸。对离体胃条有短暂收缩兴奋，随即转入抑制，降低胃张力和拮抗乙酰胆碱引起的胃收缩。     

胃血止糊剂对大鼠实验性胃溃疡的影响

目的：探讨胃血止糊剂保护胃黏膜的作用及其机制,为其临床应用提供依据。方法：通过应用大鼠应激性溃疡模型、乙醇致胃粘膜损伤模型、幽门结扎胃溃疡模型,大鼠胃液分析。观察胃血止糊剂对大鼠应激性溃疡模型、乙醇致胃粘膜损伤模型、幽门结扎胃溃疡模型的影响,对大鼠胃液总量及胃酸分泌的影响。结果：胃血止糊剂有抑制溃疡形成,降低大鼠胃溃疡指数,抑制胃酸分泌,降低胃液酸度,保护胃黏膜的作用。结论：胃血止糊剂有显著保护胃黏膜,抑制胃酸分泌及溃疡的形成,防止胃出血加重的作用。     

复方小建中冲剂药理研究

复方小建中冲剂显著抑制水应激性和消炎痛加乙醇诱发的小鼠胃溃疡,抑制0.6mol/L盐酸和结扎幽门引起的大鼠胃溃疡,溃疡抑制率为41—87％;并能抑制小鼠小肠推进性运动;抑制热和醋酸引起的小鼠痛反应;同时抑制二甲苯琼脂和角叉菜胶引起的小鼠、大鼠炎症反应。     

补阳还五汤抗胃溃疡的实验研究

目的 :观察补阳还五汤抗实验性胃溃疡的作用。方法 :采用水应激法、利血平性溃疡法和幽门结扎法建立胃溃疡实验模型。分别观察补阳还五汤 0 .6 g/ kg、1.2 g/ kg不同剂量及甲氰咪胍 0 .2 g/ kg对上述各类型溃疡的抑制作用。结果 :补阳还五汤 0 .6 g/ kg对小鼠应激型溃疡、利血平型溃疡和大鼠幽门结扎型溃疡有明显的抑制作用 (P <0 .0 1或 P <0 .0 5 ) ,对胃蛋白酶活性及排出量则无明显改善 (P >0 .0 5 )。结论 :补阳还五汤可用于治疗胃溃疡。     

Gastroprotective activity of ginger zingiber officinale rosc., in albino rats

The cytoprotective and gastric anti-ulcer studies of ginger have been carried out in albino rats. Cytodestruction was produced by 80% ethanol, 0.6M HC1, 0.2M NaOH and 25% NaCl. Whereas gastric ulcers were produced by ulcerogenic agents including indomethacin, aspirin and reserpine, beside hypothermic restraint stress and by pylorus ligated Shay rat technique. The results of this study demonstrate that the extract in the dose of 500 mg/kg orally exert highly significant cytoprotection against 80% ethanol, 0.6M HC1, 0.2M NaOH and 25% NaCl induced gastric lesions. The extract also prevented the occurrence of gastric ulcers induced by non-steroidal anti-inflammatory drugs (NSAIDs) and hypothermic restraint stress. These observations suggest cytoprotective and anti-ulcerogenic effect of the ginger.     

Anti-ulcerogenic properties of the aqueous and methanol extracts from the leaves of Solanum torvum Swartz (Solanaceae) in rats

ETHNOPHARMACOLOGICAL RELEVANCE: Solanum torvum (Solanaceae) is a plant currently used in Cameroon ethnomedicine for the treatment of stomach ailments. AIM OF THE STUDY: The present study was undertaken to determine the anti-ulcer potential of the aqueous and methanol extracts from the leaves of Solanum torvum. MATERIALS AND METHODS: The aqueous and methanol extracts from the leaves of Solanum torvum were tested orally at the doses of 250, 500 and 750mg/kg, on gastric ulcerations experimentally induced by HCl/ethanol, indomethacin, pylorus ligation and stress. The fractionation of the methanol extract through silica gel column chromatography produced seven different fractions (A-G) which were tested orally at the dose of 100mg/kg against HCl/ethanol-induced ulceration. RESULTS: The methanol extract at the dose of 750mg/kg produced 98.12, 99.16, 98.70 and 96.03% inhibition when gastric ulcerations were induced by HCl/ethanol, indomethacin, pylorus ligation and stress, respectively. The aqueous extract at the same dose produced 96.55, 96.86, 98.63 and 98.63% inhibition on ulcerations induced respectively by HCl/ethanol, indomethacin, pylorus ligation and stress. All the fractions of the methanol extract significantly inhibited ulcer formation. Fraction F which contains flavonoids and triterpens was the most active and exhibited an inhibitory percentage of 84.74. Both extracts significantly increased mucus production and reduced gastric acid secretion. CONCLUSIONS: The aqueous and methanol extracts of the leaves of Solanum turvum possess anti-ulcerogenic properties that may be due to cytoprotective mechanism. These results support the ethnomedical uses of the plant in the treatment of gastric ulcer.     

Effect of Commiphora opobalsamum (L.) Engl. (Balessan) on experimental gastric ulcers and secretion in rats

The ulcer protective potential of an ethanol extract of Commiphora opobalsamum (L.) Engl. (Burseraceae) 'Balessan' was assessed against different acute gastric ulcer models in rats induced by necrotizing agents (80% ethanol, 0.2M NaOH and 25% NaCl), hypothermic restraint stress, pyloric ligation (Shay) and indomethacin. Balessan, 250 and 500 mg/kg administered orally (intraperitoneally in Shay rat model) showed a dose-dependent ulcer protective effects in all the above ulcer models. Besides, the extract offered protection against ethanol-induced depletion of stomach wall mucus and reduction in nonprotein sulfhydryl (NP-SH) concentration. Ethanol treatment also caused histopathological lesions of the stomach wall. Pretreatment with Balessan extract provided a complete protection of gastric mucosa through supporting both the offensive and defensive factors. Balessan extract was also showed a large margin of safety without any apparent adverse effects in rats.     

Evaluation of the antipeptic ulcer activity of the leaf extract of Plantago lanceolata L. in rodents

The effect of the leaf extract of Plantago lanceolata L. (Plantaginaceae) on gastric secretion and cytoprotection was evaluated using different models of gastroduodenal ulcer, including acetic acid induced chronic gastric ulcer, indomethacin induced gastric ulcer, cysteamine induced duodenal ulcer and pylorus ligation induced gastric ulcer. The aqueous extract was administered at 200 mg/kg and 400 mg/kg and 140 mg/kg and 280 mg/kg for mice and rats, respectively, and compared with vehicle or the standard, ranitidine (50 or 70 mg/kg) or misopristol (280 μg/kg). In addition, activity of the mucilage (172 mg/kg) was also evaluated in acetic acid induced chronic gastric ulcer. Administration was done orally except in pylorus ligation, where the intraduodenal route was used. In all cases, higher doses of the extract provided better protection than lower doses and the mucilage, hinting at a dose‐dependent effect. Whilst higher doses of the extract showed a better healing of the ulcer as well as protection in indomethacin and pylorus ligation models, activities of lesser magnitude than ranitidine were noted in the cysteamine model. Together these findings indicate that higher doses used in the present study provided an overall better protection against gastroduodenal ulcers than the standard drugs employed through antisecretory and cytoprotective mechanisms. Copyright © 2011 John Wiley & Sons, Ltd.     

复方丹蒲汤抗实验性胃溃疡的作用

目的:研究复方丹蒲汤(丹参、蒲公英、砂仁等)抗实验性胃溃疡的作用及其机制。方法:采用小鼠水浸应激性、利血平性、无水乙醇性、幽门结扎性胃溃疡模型观察复方丹蒲汤对胃溃疡的保护作用及对大鼠胃液分泌的影响;采用小鼠胃排空方法观察复方丹蒲汤对胃运动的影响;以热板法观察其镇痛作用。结果:复方丹蒲汤对小鼠水浸应激性、利血平性及无水乙醇性胃溃疡模型均有明显的保护作用;能抑制大鼠胃酸及胃蛋白酶的分泌,降低大鼠胃溃疡指数,减少溃疡面积;抑制小鼠胃排空;延长小鼠热刺激的痛反应时。结论:复方丹蒲汤具有抗实验性胃溃疡的作用。该作用可能与其抑制胃液分泌、胃平滑肌运动及镇痛作用有关。     

沙棘果肉油与籽油抗胃溃疡作用的比较及其机制研究

目的：比较研究沙棘果肉油与籽油抗胃溃疡作用并对其机制进行初步探讨。方法：采用大鼠水浸应激性、利血平型、幽门结扎型及乙酸型胃溃疡模型研究比较沙棘果肉油和籽油抗胃溃疡作用；以幽门结扎收集胃液观察对胃液分泌的影响；以甲基橙比色法观察对胃排空的影响；以热板法和扭体法观察其镇痛作用。结果：沙棘果肉油和籽油对大鼠水浸应激性、利血平型、幽门结扎型胃溃疡均有明显的保护作用；对大鼠乙酸型胃溃疡均有明显的促进愈合作用；能抑制大鼠胃酸及胃蛋白酶的分泌，促进胃粘液的分泌；能抑制正常小鼠胃排空；可减少小鼠的扭体次数。结论：沙棘果肉油和籽油均有抗实验性胃溃疡的作用，两者作用强度相似。该作用可能与其抑制胃液分泌，促进胃粘液分泌，抑制胃平滑肌运动及镇痛作用有关。     

Effect of Manilkara hexandra (Roxb.) Dubard against experimentally-induced gastric ulcers

Effects of the flavonoid rich fraction of the stem bark of Manilkara hexandra (Roxb.) Dubard, have been studied on ethanol, ethanol-indomethacin and pylorus ligated gastric ulcers in experimental animals. Oral administration of the ethyl acetate extract (extract A3) inhibited the formation of gastric lesions induced by ethanol in a dose dependent manner. The protective effect of extract A3 against ethanol induced gastric lesions was not abolished by pretreatment with indomethacin (10 mg kg(-1)). Further, extract A3 inhibited increase in vascular permeability due to ethanol administration. Extent of lipid peroxidation was significantly reduced in animals treated with extract. Extract A3 also inhibited the formation of gastric ulcers induced by pylorus ligation, when administered both orally and intraperitoneally. Moreover, pretreatment with extract A3 increased mucus production and glycoprotein content, which was evident from the rise in mucin activity and TC: PR ratio.