槟榔提取物对人类口腔黏膜上皮角朊细胞分泌内皮素的影响

目的 通过观察槟榔提取物(areca nut extract, ANE)对体外培养的人类口腔黏膜上皮角朊细胞(keratinocytes, KC)分泌内皮素(endothelin, ET)的影响， 初步探讨KC和ET在口腔黏膜下纤维性变(oral submucous fibrosis, OSF)发生中的作用。方法 分别从正常(NM)及OSF的口腔黏膜组织中培养出KC， 向培养基中加入不同浓度的ANE， 用放射免疫法测定培养细胞上清液中ET的水平。结果 OSF－KC 48 h的ET分泌量为96.983±17.802 pg/ml， 高于NM－KC分泌量14.641±15.796 pg/ml(P＜0.05)； ANE在5～150　μg/ml范围内以浓度—效应依赖关系刺激KC分泌ET。结论 人类口腔黏膜上皮KC能分泌一定量的ET， 且OSF－KC分泌的ET水平高于NM－KC， ANE能刺激KC分泌ET， 提示ANE可能是一种能引起KC分泌ET水平上调的外源性刺激物， ANE可能是通过改变KC分泌细胞因子的水平而诱发OSF。     

槟榔提取物对口腔黏膜角朊细胞分泌肿瘤坏死因子α的影响

目的 :观察槟榔提取物对口腔黏膜角朊细胞分泌肿瘤坏死因子α的影响 ,探讨槟榔诱发口腔黏膜下纤维性变的机制。方法 :通过体外培养口腔黏膜角朊细胞 ,用不同浓度槟榔提取物对其进行干预 ,放射免疫方法检测不同干预时段培养上清中的肿瘤坏死因子α浓度。结果 :槟榔提取物干预角朊细胞 12h后肿瘤坏死因子α的量开始增加 ;并以剂量依赖关系增加角朊细胞产生肿瘤坏死因子α的量。结论 :槟榔提取物可能通过促进角朊细胞合成肿瘤坏死因子α诱发口腔黏膜下纤维性变。     

槟榔提取物对人类口腔黏膜上皮角朊细胞分泌内皮素的影响

目的通过观察槟榔提取物(areca nut extract,ANE)对体外培养的人类口腔黏膜上皮角朊细胞(keratinocytes,KC)分泌内皮素(endothelin,ET)的影响,初步探讨KC和ET在口腔黏膜下纤维性变(oral submucous     

槟榔提取物抑制人类口腔粘膜角朊细胞生长的实验研究

目的：建立人类口腔粘膜上皮角朊细胞体外原代培养方法：探讨口腔粘膜下纤维性变患者上皮厚度改变的机理;方法;先对人类口腔粘膜上皮角朊细胞进行分离,培养和鉴定,然后用四唑盐比色试验检测ＯＳＦ患者和正常人口腔粘膜上皮ＫＣ增殖状况,并且观察槟榔提取物对ＫＣ增殖的影响。     

槟榔提取物抑制人类口腔黏膜成纤维细胞生长的实验研究

目的通过比较正常口腔黏膜和口腔黏膜下纤维性变(OSF)组织中成纤维细胞(FB)增殖差异、检测槟榔提取物(ANE)对成纤维细胞增殖的影响,来探讨OSF的发病机理.方法对人类口腔黏膜成纤维细胞进行分离培养,然后用四唑盐(MTT)比色试验法检测OSF患者和正常人口腔黏膜FB增殖状况,并且观察ANE对FB增殖的影响.结果表示增殖水平的OD值在OSF-FB为0.254±0.045,高于NM-FB的OD值0.236±0.012(P<0.05),ANE以浓度-效应依赖关系抑制FB增殖.结论 OSF-FB细胞增殖率较NM-FB高;ANE对口腔黏膜FB有细胞毒作用,提示槟榔及其有效成分不完全是通过直接刺激FB增殖而诱发OSF.     

槟榔提取物抑制人类口腔黏膜成纤维细胞生长的实验研究

目的通过比较正常口腔黏膜和口腔黏膜下纤维性变(OSF)组织中成纤维细胞(FB)增殖差异、检测槟榔提取物(ANE)对成纤维细胞增殖的影响,来探讨OSF的发病机理.方法对人类口腔黏膜成纤维细胞进行分离培养,然后用四唑盐(MTT)比色试验法检测OSF患者和正常人口腔黏膜FB增殖状况,并且观察ANE对FB增殖的影响.结果表示增殖水平的OD值在OSF-FB为0.254±0.045,高于NM-FB的OD值0.236±0.012(P<0.05),ANE以浓度-效应依赖关系抑制FB增殖.结论 OSF-FB细胞增殖率较NM-FB高;ANE对口腔黏膜FB有细胞毒作用,提示槟榔及其有效成分不完全是通过直接刺激FB增殖而诱发OSF.     

槟榔碱对口腔颊黏膜成纤维细胞和血管内皮细胞增殖活性的影响

目的：比较槟榔碱对NM—FB、OSF—FB、EC增殖活性的影响。方法：0μ／ml、30μm／ml、60μg／ml、90μg／ml、120μg／ml槟榔碱干预三组细胞48h,60μg／ml槟榔碱干预三组细胞24h、48h、72h,倒置显微镜下观察细胞形态的改变,四唑盐（MTT）比色法检测细胞增殖活性的变化。结果：槟榔碱以浓度、时间-依赖关系抑制细胞增殖,相同条件下存活率EC低于NM—FB、NM—FB低于OSF—FB。结论：槟榔碱有细胞毒作用,细胞损伤有先后时序关系;推测槟榔碱并不直接通过刺激FB增殖诱发OSF,可能通过改变FB性能和EC活性促进OSF的发生。     

血管内皮细胞上清液对口腔颊黏膜成纤维细胞增殖活性的影响

目的：观察血管内皮细胞上清液（ECS）对正常人口腔颊黏膜成纤维细胞（NM-FB）增殖活性的影响。方法：收集不同时间点、并配成不同比例的ECS干预NM-FB。结果：ECS12h能促进NM-FB的增殖,随着时间延长ECS促增殖能力逐渐增强,ECS48h促FB增殖能力达到最大（P〈0.05）;ECS/（ECS＋RPMI-1640）比值为1/4时可促NM-FB增殖,ECS稀释倍数越小,NM-FB的增殖率越高（P〈0.05）。ECS能促进FB的增殖,存在时效和量效依赖关系。结论：血管内皮细胞能通过分泌某些介质影响口腔颊黏膜成纤维细胞的生物学活性。     

口腔黏膜下纤维性变发病机制的研究进展

口腔黏膜下纤维性变 (OSF)的发病机理尚不清楚 ,近年来研究发现 :口腔黏膜角朊细胞、多种细胞因子、铜、细胞免疫等因素与OSF的发生密切相关 ,本文就这些因素在OSF发生发展过程中的可能作用作一综述。     

口腔黏膜下纤维性变癌变的回顾性研究

目的 :探讨湖南地区口腔黏膜下纤维性变 (OSF)癌变情况。方法 :对近 2 0年来就诊于湖南数家大医院 1166名OSF病例及其癌变病例进行分析。结果 :1166例OSF患者 ,有 2 0例伴有口腔鳞状细胞癌 ,癌变率为1.7% ,其中男 18例 ,女 2例 ,癌变病例主要来源于湘潭地区 ,均为长期咀嚼槟榔者 ,18例 (90 % )有 15年以上吸烟史 ,癌变部位主要位于颊部 ,患者均存在不同程度的张口受限。另外 ,还有 74例 (6.3 % )伴有口腔白斑 ,3 7例(3 .2 % )伴有口腔扁平苔藓。结论 :OSF是一种与嚼槟榔有关的癌前状态 ,可以出现癌变。     

槟榔提取液对血管内皮细胞分泌内皮素 1 的影响

目的:观察在不同浓度槟榔提取液(aqueous areca nut extract,AANE)作用下血管内皮细胞(endothelialcell,EC)分泌内皮素 1(endothelin-1,ET-1)的变化,探讨 EC 及 ET-1 在口腔黏膜下纤维性变(oral submucous fibro-sis,OSF)发生发展中的作用.方法:体外培养人脐静脉内皮细胞,换入含终浓度分别为0、50、100、200、400、600、800μg/ml AANE 的无血清 DMEM 培养基,48 h 后用放射免疫法检测细胞培养上清液中 ET-1 的水平.结果:100μg/ml及以上浓度的AANE作用后,EC 分泌 ET-1 的量较未加 AANE 组显著增加,并呈浓度依赖性.结论:一定浓度的AANE 能使 EC 异常分泌 ET-1,可能参与OSF病理过程的发生发展.     

丹参注射液对槟榔提取液所致血管内皮细胞损伤的保护作用

目的：研究丹参注射液对槟榔提取液（aqueous areca nut extract,AANE）所导致的血管内皮细胞（endothelial cell,EC）损伤的保护作用,旨在为丹参注射液治疗口腔黏膜下纤维性变（oral submucous fibrosis,OSF）提供理论依据。方法：体外培养人脐静脉EC,分为保护组及预防组2组,分别在AANE作用前后将EC与丹参注射液共同培养,MTT法检测EC增殖,放免法检测EC分泌内皮素1（endothelin—1,ET—1）量的变化。结果：①在AANE干预之后将丹参注射液与EC共同培养,与对照组相比,EC增殖情况明显增加（t=-2．805,p〈0．05）,分泌ET—1的量显著减少（t=2．965,p〈0．05）。②在AANE干预之前先将丹参注射液与EC共同培养,发现EC增殖（t=2．701,p〉0．05）分泌ET—1的量（t=-1．541,p〉0.05）与对照组相比差异无统计学意义。结论：丹参注射液能有效抑制AANE导致的EC损伤,使EC分泌ET—1减少濑刽Ⅲ能通过ET—1这个靶标来发挥抗纤维化作用。     

Prevalence of oral submucous fibrosis among areca nut chewers: A systematic review and meta-analysis

Background: Worldwide millions peoples consume AN who are at risk of OSMF. Prevalence of OSMF is reported between 0.03% and 30% irrespective of AN habit. Further, these estimates are based on sample population comprised of OSMF patients or general population rather AN chewers (ANC). Therefore, available evidence does not reflect the true prevalence of OSMF among ANC. Method: The studies providing the prevalence of OSMF in ANC were identified in PubMed, Scopus, and Web of Science. Pooled prevalence and quality assessment using New-Ottawa Scale were performed. Results: Fifteen studies reported the prevalence of OSMF (929) in ANC (53,213). Most studies were from China (six studies) and India (four studies) correlating with regions having high ANC. The pooled prevalence of OSMF in ANC was 5% (0.05 [95% CI, 0.03, 0.08]). All studies' quality was satisfactory; however, the OSMF diagnosis method, age, gender, and habits need further scrutiny. Conclusion: Available evidence suggested a low prevalence of OSMF in ANC, although further large-scale studies are recommended to validate this finding. Understanding the prevalence and distribution patterns of OSMF might aid intervention healthcare programs and contribute to the reduction of the oral cancer burden related to OSMF.     

Growth of oral and skin fibroblasts from patients with oral submucous fibrosis

The purpose of the investigation was to evaluate and compare the proliferation (growth) of mouth fibroblasts and skin fibroblasts from patients with oral submucous fibrosis (OSF). Material comprised fibroblasts from fibrous bands situated in the buccal mucosa and from the inner aspect of the forearm of 8 patients with classic features of OSF as well as fibroblasts from 6 buccal mucosa and 8 skin biopsy specimens from healthy non-areca nut chewing individuals. Cells were cultured for 8 days according to standard techniques. Their growth was monitored daily, under optimal conditions as well as exposure to concentrations of arecoline. The data were analyzed using regression analysis, analysis of variance and the Kruskal-Wallis test. We found no statistically significant differences between the proliferation patterns of oral and skin fibroblasts from patients or between those from patients and controls. The reaction of the cells exposed to concentrations of arecoline was similar; at low concentrations (0.1–10 μg/ml) normal growth was maintained, while 100 μg/ml inhibited growth. It is concluded that fibroblasts from mouths affected by OSF have proliferation patterns which fall within normal parameters, that the excessive collagen formation in established OSF is not due to increased fibroblast proliferation and that arecoline does not stimulate fibroblast proliferation.     

Etiology of oral submucous fibrosis with special reference to the role of areca nut chewing

Oral submucous fibrosis (OSF) is a high risk precancerous condition, predominantly affecting Indians. Consumption of chilli was hypothesized as an etiologic factor on the basis of ecological observations and a solitary animal experimental study. Subsequent epidemiologic studies that included case-series reports, large cross-sectional surveys, case-control studies, cohort and intervention studies have identified areca nut as the major etiologic agent. Tissue-culture studies involving human fibroblasts, areca nut extracts and areca nut alkaloids supported this etiologic hypothesis by showing fibroblastic proliferation and increased collagen formation. Currently, the role of genetic susceptibility and that of autoimmunity are receiving attention. The influence of nutritional factors, if any, remains unclear.     

槟榔提取物对内皮细胞分泌一氧化氮的影响

目的 :探讨口腔黏膜下纤维性变 (OSF)可能的发病机理。方法 :用槟榔提取物干预体外培养的内皮细胞 ,收集培养上清 ,用ELISA方法检测内皮细胞分泌一氧化氮 (NO)的浓度。结果 :槟榔提取物能抑制体外培养的内皮细胞分泌NO。结论 :OSF的发生可能与槟榔引起的内皮细胞合成NO减少有关。     

槟榔与细胞内活性氧及自噬的关系

槟榔作为一级致癌物,其与口腔癌的关系受到广泛关注。活性氧（ROS）及自噬水平与肿瘤的发生发展密切相关,研究发现槟榔可诱发细胞内ROS及自噬水平变化。本文结合相关研究进展,对槟榔、细胞内ROS以及自噬之间的关系进行综述。