Ziehl-Neelsen改良染色法在痰涂片中的应用

针对结核杆菌菌体含有蜡质的特点和传统Ziehl Neelsen抗酸染色法存在的不足 ,笔者在以前工作的基础上对染色液的配方进行改良〔1,2〕,并结合微波辐射技术建立了一种快速、敏感、简便的痰涂片抗酸染色法。1　材料与方法1.1　材料　 5 3例结核杆菌痰培养阳性的痰液 ,每例涂片 2张 ,95 %乙醇固定 15～ 30min ,分别用Ziehl Neelsen改良染色法和传统的抗酸染色法染色 ,同时设阳性对照、阴性对照。1.2　染液配制　 (1)改良的Ziehl Neelsen染色液 :碱性复红无水乙醇饱和液 (相当 6 %浓度 ) 10ml,活性剂 5ml,蒸馏水85ml,先将碱性复红溶于无水乙醇…     

结核杆菌的基因诊断技术进展

结核病于80年代后期出现世界范围内的回升,并与艾滋病相互促进发病.快速、灵敏、准确地诊断结核病势在必行。采用基因诊断技术可以满足这项要求。本文对结核杆菌基因诊断的序列、技术和方法进行综述。     

多聚酶链反应（PCR）检测不典型结核杆菌的DNA

本文采用ＰＣＲ技术检测结核杆菌的ＤＮＡ（ＴＢ－ＤＮＡ），其中临床诊断为结核或可疑结核者９例，肿瘤６例，结节病３例，淋巴结炎４例，共２２例。病理诊断确诊为不典型结核者１４例，不能除外结核者４例，结节病２例，瘤样病变２例。所有病例经抗酸染色均未查到结核杆菌。ＰＣＲ检测结果表明，２２例中１８例呈阳性（占８１．８２％），４例呈阴性。ＰＣＲ技术敏感、特异，对于不典型结核病例检查ＴＢ－ＤＮＡ片段用于辅助诊断颇     

联用两种检测抗结核杆菌抗体的方法诊断结核病

目前,通过检测抗结核杆菌的抗体（ＩｇＧ）诊断结核病时有报道,已商品化的试剂盒也较多。采用的方法包括:ＥＩＡ、胶体金标记的免疫层析法和胶体金标记的渗滤法等,采用的抗原也各不相同。这些方法单独使用时,其检测的特异性和灵敏度都有些缺点。为了探讨较理想的结核病的辅助诊断方法,我们将包被抗原为结核杆菌外膜蛋白的金标渗滤试剂和包被抗原为ＰＰＤ的快速ＥＬＩＳＡ诊断试剂,两种检测抗结核杆菌抗体的方法联合应用,观察了其对结核病的诊断价值。１材料和方法１．１对象分为两组:①实验组:为１２７例活动性肺结核患者,男７２例,女５５例…     

PCR技术检测活检组织中结核杆菌DNA

ＰＣＲ技术检测活检组织中结核杆菌ＤＮＡ赵跃武银平章刘正国郝远瑞徐纪跃党伟一作者单位：河南省人民医院病理科，郑州４５０００３多聚酶链反应（ＰＣＲ）是一项有效的体外ＤＮＡ扩增技术，尤在病原体检测方面。在一些可疑的活检石蜡切片中，有时也需要借助于该技术来协...     

神经原纤维Bodian染色法的改良

Ｂｏｄｉａｎ染色法[1] 于 1936年首创 ,是一种用于观察神经元、轴突和树突内神经原纤维变化的浸银染色法。基本原理是把甲醛固定后的石蜡切片浸于银溶液中 ,再用还原液处理后 ,使银颗粒沉积于神经纤维上呈现为棕色或棕黑色。传统染色液的配制是在蛋白银溶液内放入金属铜。但此方法存在着以下几个缺陷 :(1)背景色深 ,血管和胶原纤维有共染现象 ,影响对神经纤维的观察。 (2 )染色时间难于掌握。 (3)容易脱片。我们对经典Ｂｏｄｉａｎ染色法进行了改良 ,建立了一种去铜改良型Ｂｏｄｉａｎ染色法 ,并对其应用进行了探索。一、材料与染色步骤1.…     

肥大细胞甲苯胺蓝-PAS改良染色法

肥大细胞（mast cell）是疏松结缔组织中常见的细胞,位于小血管附近,成行成群分布,胞体较大,呈圆或卵圆形,核小而圆,染色深,居中,其胞质中充满粗大的嗜碱性颗粒,有异染性,颗粒易溶于水,颗粒内含有肝素、组胺和嗜碱性粒细胞趋化因子等[1]。肥大细胞染色方法种类很多[2],常用甲苯胺蓝（tolui-dine blue）染色。     

胸水游离DNA的结核杆菌检测在结核病诊断中的价值北大核心CSCD

结核病（tuberculosis）是严重的传染病之一,目前临床有多种方法检测结核分枝杆菌,如血清抗体检测法,痰涂片抗酸染色法、细菌培养法,荧光PCR法等。每种方法都有其优缺点,如血清抗体检测灵敏度高但特异度差,细菌培养法耗时长,痰涂片抗酸染色法快捷但灵敏度差等。     

改良抗酸染色法诊断盆腔结核意义分析

目的 建立改良抗酸染色法并分析其在盆腔结核中的诊断价值.方法 对48例盆腔结核患者盆腔积液用离心涂片法、改良抗酸染色法和BACTEC MGIT 960培养法进行检测,并对结果进行分析.结果 离心涂片法、改良抗酸染色法和BACTEC MGIT 960培养法阳性率分别为2.08％ (1/48)、39.58％ (19/48)和37.50％ (18/48),改良抗酸法分别与离心涂片法及BACTEC MGIT 960培养法比较,改良抗酸染色法的阳性检出率最高,其与离心涂片法差异有统计学意义(P＜0.001),但与BACTEC MGIT 960培养法差异无统计学意义(P＞0.05).结论 改良抗酸染色法较离心涂片法阳性率明显提高,虽然和BACTEC MGIT 960培养法阳性率无明显差异,但是改良抗酸染色法可以快速诊断盆腔结核,对于临床工作有重要意义.     

A modified alpha-galactosyl ceramide for staining and stimulating natural killer T cells

CD1d presentation of alpha-galactosyl ceramides to natural killer T cells has been a focal point of the study of regulatory T cells. KRN7000, an alpha-galactosyl ceramide originally generated from structure activity studies of antitumor properties of marine sponge glycolipids, is currently the most commonly used agonist ligand and is used to stain NKT cells. However, this glycolipid suffers from poor solubility and availability. We have developed an alpha-galactosyl ceramide with improved solubility over KRN7000 that effectively stains NKT cells, both mouse and human, and stimulates cytokine release at low concentrations.     

改良网织红细胞染色液的应用评价

目的评价一种改良的网织红细胞染色液。方法对改良网织红细胞染色液进行显微镜镜下观察试验、染色时间试验、有效期试验、准确度试验和精密度试验。结果改良网织红细胞染色液染色后的标本在显微镜镜下具有不易褪色、沉渣少、视野清晰、背景明亮等特点,且改良网织红细胞染色液染色时间短（5min）,有效期长（30个月）,准确度与煌焦油蓝盐水染色液一致（P〉0．05）,精密度好于煌焦油蓝盐水染色液。结论改良网织红细胞染色液是一种优秀的网织红细胞染色液,值得推广应用。     

A human antiserum reacting with modified blood group M determinants.

In the serum of a healthy blood donor, Mar, antibodies were identified that agglutinate blood group M erythrocytes only after their preincubation in glucose-containing solution. This agglutination was inhibited by blood group M glycoprotein preincubated in glucose solution but not by untreated M and N glycoproteins or N glycoprotein pretreated with glucose. A similar "activating' effect on M erythrocytes or M glycoprotein was achieved when glucose was replaced by mannose or N-acetylglucosamine, whereas several other replaced by mannose or N-acetylglucosamine, whereas several other sugars were ineffective. The "activating' effect of glucose on M antigen was time-, temperature-, pH-, and concentration-dependent. The binding of glucose to M glycoprotein was demonstrated under the preincubation conditions used in the above mentioned agglutinations. The results obtained suggest that the antibodies of serum, Mar, react with blood group M determinants modified by formation of a glycosylamine linkage between amino groups of the glycoprotein and glucose or other hexoses with the same configuration at asymmetric carbons 3, 4 and 5 as glucose.     

A method for staining multiple chemotaxis filters.

A device is described which holds chemotaxis filters on glass slides so that the cells on as many as 128 filters can be stained simultaneously. The procedure for using the batch stainer with Wright's stain is outlined.     

A modified silver methenamine Masson trichrome stain using methyl green for staining of renal biopsies

Renal pathology uses a battery of stains to allow proper assessment of all renal components. One of the most useful of these stains is the silver methenamine with a Masson trichrome counterstain (SMMT). The SMMT stain uses thin 1-um sections to identify immune complexes deposits on the basement membranes and in the mesangium of the glomerulus and can provide an excellent and inexpensive method. Problems can, however, occur when the stain is not performed by a technician or the stain needs to be performed in large numbers. A variation to the SMMT method has proved to provide a better, more robust and reproducible counterstain. The new method substitutes Light Green SF, used in the traditional Masson trichrome stain, with Methyl Green, a dye with much smaller molecule size, allowing better penetration and a stronger bonding to the tissue components.