进展期白癜风外周血T淋巴细胞及其亚群的检测

白癜风是一种常见的皮肤病 ,局部色素脱失 ,其病理过程还未完全阐明 ,但目前提出三种假说 :一是自身免疫学说 ;二是神经体液学说 ;三是黑素细胞自身破坏学说[1 ] 。目前以自身免疫学说尤为人们所重视。支持这一学说的证据 :自身免疫病患者发生白癫风较一般人群高 10～ 15倍 ;白癜风患者血清中抗甲状腺、抗平滑肌和抗胃壁细胞等器官特异性自身抗体发生率增高 ;黑素细胞瘤患者白癜风的发生率明显高于正常人 ;84 %的白癜风患者血清中存在白癜风抗体。已有一些关于白癜风外周血淋巴细胞的报导 ,但结果不一致[2 - 6 ] ,我们实验的目的是用间接免…     

白癜风患者外周血T淋巴细胞亚群检测及其意义

用单克隆抗体技术测定 5 2例进展期白癜风患者及 32例正常人血中T淋巴细胞亚群 ,并用流式细胞仪进行定量分析。结果 :①与正常人相比 ,进展期白癜风患者外周血CD4 + T细胞明显减少 ,CD8+ T细胞增加 ,CD4 + CD8+ 值降低 ,存在明显差异 (P <0 .0 1)。②各类型白癜风中 ,泛发型CD8+ T细胞增加明显 (P <0 .0 0 1)而局限型的T淋巴细胞亚群变化不明显 (P >0 .0 5 )。进展期白癜风患者外周血T淋巴细胞失调 ,细胞免疫功能异常 ,支持白癜风患者体内存在自身免疫功能异常学说。     

T细胞亚群与白癜风的研究进展

白癜风是一种常见的后天性色素脱失性皮肤黏膜病,黑素细胞的破坏在白癜风的病理中占主要地位。现认为白癜风的发病主要有自身免疫、遗传缺陷、氧化过激、微量元素失衡等有关[1],免疫机制是导致白癜风发病的重要因素[2]。T细胞是免疫细胞的重要组成部分,是皮肤免疫的主要效应细胞,可以通过不同的途径导致黑素细     

中药对肝肾不足型白癜风外周血T淋巴细胞亚群观察

目的 探讨中药治疗白癜风的疗效及作用机制.方法 采用随机、对照方法 .试验组内服中药1次/d;对照组外用肤轻松软膏,2次/d,两组均治疗6个月,观察皮疹情况.同时试验纽治疗前、后检测外周血T淋巴细胞亚群,并作统计.结果 试验组总有效率78.38%;对照组总有效率56.52%.两组总有效率比较P<0.05,差异有统计学意义.试验组治疗后CD3、CD4、CD4/CD8比值均增高.结论 中药治疗白癜风疗效好,可能与提高免疫力有关.     

尖锐湿疣患者免疫功能的研究进展

尖锐湿疣患者的外周血T淋巴细胞亚群和NK细胞异常及细胞因子产生失衡所引起一系列细胞免疫反应的抑制，全身和局部单核-巨噬细胞功能障碍。而这也与复发有关。     

小剂量皮质类固醇等综合治疗白癜风疗效观察

采用小剂量泼尼松及转移因子等综合疗法治疗67例白癜风,结果痊愈33例(49.25%),显效20例(29.85%),有效9例(13.43%)。痊愈率及总有效率与对照组比较,P均<0.05,有显著性差异。治疗组25例患者T淋巴细胞亚群检测结果与健康对照组比较CD3+、CD4+、CD4+/CD8+明显降低,P<0.01,而CD8+则升高,P<0.01。对其中20例白癜风患者治疗前后动态T淋巴细胞亚群检测结果显示,治疗后CD3+、CD4+、CD4+/CD8+均有明显升高,P<0.01,而CD8+则有下降,P<0.05。     

208例儿童白癜风患者外周血免疫球蛋白、补体及T淋巴细胞亚群分析

目前越来越多的证据表明,白癜风的发病与自身免疫有关^[1-3].为探讨儿童白癜风与免疫的关系,我们检测了208例儿童白癜风患者外周血免疫球蛋白、补体和T淋巴细胞亚群的水平,现分析如下.     

白癜风患者外周血T淋巴细胞亚群和sIL-2R水平变化的研究

采用ＡＰＡＡＰ法和双缺本夹心ＥＬＩＳＡ技术检测１５０例白癜风患者外周血Ｔ淋巴细胞亚群及可溶性白介素－２受体（ｓＩＬ－２Ｒ）水平。结果显示：（１）寻常型白癜风外周血ＣＤ３＾＋、ＣＤ４＾＋细胞数、ＣＤ４＾＋／ＣＤ８＾＋比值明显低于正常对照（Ｐ〈０．０１）,ｓＩＬ－２Ｒ活性显著性高于节段型白癜风和正常人（Ｐ〈０．０１）;节段型白癜风ＣＤ４＾＋、ＣＤ４＾＋／ＣＤ８＾＋与正常对照组比较也显著减少（Ｐ〈０．０     

中医药治疗60例进展期白癜风临床研究

目的 观察三组复方中药治疗进展期白癜风的临床疗效,优选治疗方案.方法 对入组的147例病例中医辨证后选方观察疗效,每月复诊1次,3个月为1个疗程,对完成的60例有效病例进行分析.结果 进展期白癜风以风血相搏证多见,中医治疗进展期白癜风有效率为70.00%,三组中药控制进展期白癜风无统计学差异,不同部位疗效不同,面部治疗有效率达到93.75%,而肢端治疗有效率为0.00%.结论 三种复方中药治疗进展期白癜风有较好疗效.     

1367例局限型白癜风的回顾性分析

目的 总结和分析局限型白癜风进展的临床特征.方法 采取调查问卷方式收集1 367例局限型白癜风门诊患者的临床资料,进行整理,统计分析局限型白癜风进展的临床特征.结果 由局限型发展到散发型白癜风782例(57.21％),平均4.701年;由局限型进展到肢端型白癜风199例(14.56％),平均6.721年;局限型发展为节段型191例(13.97％),平均2.034年;局限型发展为泛发型33例(2.41％),平均7.18年;有162例(11.85％)仍是局限型白癜风.结论 大多数局限型白癜风是寻常型白癜风的早期,少部分是节段型白癜风的早期.随着时间的进展以及相关可能诱因的影响,进展为散发型白癜风占多数.局限型白癜风平均经过7～8年,能进展为泛发型白癜风.因此,白癜风应尽早治疗,控制其发展.     

Vitiligo: new and emerging treatments

ABSTRACT:  Vitiligo is a cosmetically disfiguring condition, and, although there is no therapeutic full solution yet, some treatment may induce good results in most patients. The disease can be successfully treated with various medical options. Both nonfocused or focused narrowband ultraviolet B phototherapy represents the current treatment of choice, to minimize side effects and reach optimal clinical results. Topical novel approaches are also considered. Surgical methods, consisting of autologous transplantation methods, is generally recommended for focal/stable vitiligo, after medical therapy has failed. Finally, for patients with extensive vitiligo, depigmentation of the residual melanin should be taken into account.     

Vitiligo associated with malignant melanoma and lupus erythematosus

There exists several reports where malignant melanoma is associated with vitiligo, vitiligo with discoid lupus erythematosus and lupus erythematosus with urticaria. However, there are no reports in which vitiligo, malignant melanoma, lupus erythematosus and urticaria coexist in the same case. Herein, we report a case of a patient who developed lupus erythematosus, malignant melanoma, vitiligo and urticaria simultaneously.     

转录因子T-bet/GATA3与白癜风相关性研究

目的分析白癜风患者Th1、Th2两类细胞因子和转录因子T-bet与GATA3的相关性,探讨祛白丸对本病的治疗作用.方法32例活动期白癜风患者用祛白丸治疗前后用ELISA和RT-PCR技术分别检测血清γ干扰素(IFN-γ)、白介素4(IL-4)水平和外周血单个核细胞转录因子T-bet与GATA3的表达状况,并以10例正常人为对照.结果活动期血清IFN-γ水平较正常人降低,IL-4水平升高;T-bet下降,GATA3升高.治疗后皮疹静止或消退,血清IFN-y、IL-4、T-bet和GATA3水平与正常人相同.结论活动期白癜风患者Th1/Th2失衡,分别与T-bet表达下降,GATA3表达升高相关;祛白丸可以降低Th2类细胞因子和GATA3的表达,提高Th1类细胞因子和T-bet的表达和治疗白癜风的作用.     

Vitiligo: interplay between oxidative stress and immune system

Vitiligo is a multifactorial polygenic disorder with a complex pathogenesis, linked with both genetic and non‐genetic factors. The precise modus operandi for vitiligo pathogenesis has remained elusive. Theories regarding loss of melanocytes are based on autoimmune, cytotoxic, oxidant–antioxidant and neural mechanisms. Reactive oxygen species (ROS) in excess have been documented in active vitiligo skin. Numerous proteins in addition to tyrosinase are affected. It is possible that oxidative stress is one among the main principal causes of vitiligo. However, there also exists ample evidence for altered immunological processes in vitiligo, particularly in chronic and progressive conditions. Both innate and adaptive arms of the immune system appear to be involved as a primary event or as a secondary promotive consequence. There is speculation on the interplay, if any, between ROS and the immune system in the pathogenesis of vitiligo. The article focuses on the scientific evidences linking oxidative stress and immune system to vitiligo pathogenesis giving credence to a convergent terminal pathway of oxidative stress–autoimmunity‐mediated melanocyte loss.     

外用他克莫司与卤米松自身对照和联合治疗白癜风临床疗效分析

目的探讨外用他克莫司与卤米松自身对照、两者联合治疗白癜风的临床疗效及安全性。方法 35例选择对称或者相近部位皮损,分别给予他克莫司2次/d,卤米松1次/d,卤米松采用2周停1周的间歇疗法。8例给予他克莫司与卤米松联合治疗,他克莫司2次/d,卤米松1次/d,卤米松采用2周停1周的间歇疗法。治疗前后测量皮损面积并拍照,用于疗效对照。在共聚焦激光扫描显微镜下观察靶皮损改变。结果他克莫司和卤米松自身对照、联合治疗三者有效率差异无统计学意义。两药起效时间相近,且病程≤12个月的疗效高于病程12个月患者。两药治疗病程≤12个月疗效及病程12个月有效率差异无统计学意义。对于稳定期白癜风,卤米松有效率高于他克莫司。两药治疗进展期和稳定期白癜风有效率均差异无统计学意义。两药联合治疗对进展期白癜风皮损有较好的疗效。两种药物治疗有效的皮损在共聚焦激光扫描显微镜下可见皮损处基底层数量不一的高折光度树突状细胞,分布于毛囊或毛囊之间。结论他克莫司与卤米松、联合治疗均可以有效治疗白癜风,联合治疗并不能明显提高有效率。     

Vitiligo,Rheumatoid Arthritis and Pernicious Anemia

A patient with a 46-year history of vitiligo who also presented rheumatoid arthritis and pernicious anemia is described. Meticulous physical examination excluded further systemic or cutaneous involvement. The immunological workup revealed a low CD₄ cell percentage with T cells mostly composed of CD₈ cells, a discrepancy between the high percentage of cumulative CD₄ + CD₈ cells and the measured CD₃ proportions, very low NK cytotoxicity toward K₅₆₂ cells, and almost negligible responses to PHA, Con A and PWM mitogens. The results point to severe T and NK cell functional defects. The pathogenetic significance of these data is discussed.     

Vitiligo in children and adolescents: association with thyroid dysfunction

Background The clinical characteristics of vitiligo in children and adolescents with an emphasis on thyroid dysfunction have only been reported in a few studies. Objective The purpose of this study was to examine the characteristics of children and adolescents with vitiligo and compare the incidence of thyroid dysfunction between them and controls without vitiligo at the same age. Methods A retrospective analysis of 324 Korean children and adolescents with vitiligo was performed. The results of thyroid function screening tests in them (n = 254) were compared with controls (n = 122). Results Of the total 324 children and adolescents with vitiligo, vitiligo vulgaris was the most common type (42.3%) and the most commonly involved site was the face (54.6%). A total of 15 of 254 (5.9%) patients screened for thyroid function were diagnosed with thyroid disease (four had Hashimoto’s thyroiditis; two, Graves’ disease; seven, subclinical hypothyroidism; and two, subclinical hyperthyroidism). None of the 50 patients with segmental vitiligo showed any thyroid dysfunction (P = 0.047). There was no significant difference in the incidence of thyroid disease between children and adolescents with vitiligo and the control group, in which seven of 122 (5.7%) showed thyroid dysfunction. Conclusion In this study, we demonstrated the characteristics of children and adolescents with vitiligo and also observed no significant difference in the incidence of thyroid disease between children and adolescents with vitiligo and the control group.