中药胃肠安丸给药后木香烃内酯及去氢木香内酯药代动力学研究

采用HPLC-MS/MS多反应监测(MRM)定量分析,以地西泮为内标物在正离子模式条件下建立血样中木香烃内酯(Co)及去氢木香内酯(De)药物浓度的测定方法,探讨胃肠安丸口服给药后倍半萜内酯类成分Co及De的药代动力学过程,为进一步揭示胃肠安丸抗腹泻作用的物质基础提供理论依据。血浆样品中Co在0.700 0~769.7μg·L-1,De在2.510~956.0μg·L-1线性关系良好;且精密度、稳定性及提取回收率实验结果表明该类成分血药浓度的测定方法稳定可靠。经测定口服给药后Co及De血药浓度均随给药剂量的增加而增大,Tmax在10.65~12.98 h,表明倍半萜内酯类成分血药浓度达峰值时间较晚,Co及De Cmax分别为3.750~5.450,15.34~44.52μg·L-1;Co及De在体内吸收符合一室开放模型,其体内血药浓度达峰值所需时间较长,为揭示胃肠安丸作用物质基础及指导临床用药提供实验依据。     

84例亚急性甲状腺炎临床分析

亚急性甲状腺炎(SAT)为De Quervain于1904年首次描述,故又称De Quervain’s甲状腺炎。SAT患者多在门诊诊断和治疗,少数患者被收入院治疗。目前报道的SAT以在门诊诊治病例居多,而住院治疗患者往往     

Investigation of in vitro antioxidant activity of dihydromyricetin and flavonoids rich extract from vine tea(Ampelopsis grossedentata)

Background:Vine tea from fermented Ampelopsis grossedentata leaves has been used as a herbal tea and folk medicine in the southern region of China for hundreds of years.The aim of this investigation was to analyze the total flavonoids found in vine tea,including three bioactive flavonoids,and the total phenolic contents in the aqueous methanol extracts of 10 vine tea samples.In addition,this study also aimed to examine the antioxidant activity of dihydromyricetin and vine tea’s flavonoid-rich extract.Methods:The total flavonoids and total phenolic content assay of extracts from vine tea were performed by ultraviolet-visible spectroscopy and epoch microplate spectrophotometer,respectively.Three bioactive flavonoids were quantified simultaneously using high performance liquid chromatography.The antioxidant activity of dihydromyricetin and vine tea’s flavonoid-rich extract was evaluated in vitro using six different methods.Results:Vine tea contained a large number of flavonoids,with dihydromyricetin as its main constituent.The flavonoid-rich extract exhibited a significant scavenging effect on superoxide anion radicals,and on 3-ethylbenzthiazoline-6-sulphonic acid and 1,1-diphenyl-2-picrylhydrazyl radicals.It also possessed definite activity in lipid peroxidation inhibition,ferric reduction,and the moderation of Fe2+ion chelation ability.There was a significant negative correlation between dihydromyricetin content and antioxidant activity in the vine tea samples,including superoxide anion radical scavenging activity(P=−0.754,P<0.05),lipid peroxidation inhibition activity(P=−0.759,P<0.05),ferric-reducing antioxidant power(P=−0.843,P<0.01),respectively.Dihydromyricetin played a dominant role in the antioxidant activities of the flavonoid-rich extract.Conclusion:Vine tea’s flavonoid-rich extract could be used as a new antioxidant source to safeguard against oxidative stress.     

彩色多普勒超声在主动脉夹层诊断中的意义

目的 探讨彩色多普勒超声在主动脉夹层诊断中的价值及临床指导意义.方法 本研究纳入了33例主动脉夹层患者,以手术中所见的分型为金标准,对比分析彩色多普勒超声诊断分型结果的准确率,从而探讨其对主动脉夹层的诊断价值.结果 33例主动脉夹层患者,经彩色多普勒超声诊断为De Bakey Ⅰ型10例,De Bakey Ⅱ型4例,De Bakey Ⅲ型19例,准确率为85%.结论 彩色多普勒超声作为一种简便、快捷、无创和有效的检查方法,可以作为主动脉夹层首选检查方法,具有很高的临床指导意义.     

中药品质的研究一术

术是中医汤液疗法中不可缺少的重要生药。有来源于Atractylodes japonica Ko-idzumi和A.ovata De Candolle的白术及来源于A.lancea De Candolle及其变种的苍术。过去的研究指出,苍术有中枢神经抑制作用,其有效成分为β-按叶醇和茅术醇;朝鲜产白术有抗炎症作用,其有效成分为atractylenolideⅠ、Ⅱ、Ⅲ和eudesmadi-     

亚急性甲状腺炎的新疗法

亚急性甲状腺炎(简称亚甲炎),又称为急性非化脓性甲状腺炎、肉芽肿性甲状腺炎、病毒性甲状腺炎等。1904年由De Quervain氏作了详细报道,故又称为De Quervain氏病。一般认为本病与病毒感染有关,长期以来,国内外对本病均采用肾上腺皮质激素(强的松或地塞米松)治疗,但减药太快或停药太早则往往复发,复发率可高达50%以上,有的由于用药时间较长(在我们所诊治的患者中,服用激素     

桡骨茎突狭窄性腱鞘炎中西医治疗研究进展

正桡骨茎突狭窄性腱鞘炎是一种由于慢性劳损引起的无菌性炎症,瑞典外科医师Fritz De Quervain在1985年首次做出定义:腕背部第一伸肌肌腱室内的拇短伸肌(Extensor Pollicis Brevis,EPB)和拇长展肌(Abductor Pollicis Longus,APL)肌腱产生的炎症[1],因此该病又称为De Quervain’s tenosynovitis,目前我国男女的发病率之比约为1∶6[2]。本文就国     

银杏叶载入《美国草药典》

美国草药典委员会(AHP)将于2004年夏出版发行银杏叶干浸膏草药典(GinkgoLeaf & Ginkgo Leaf Extract),这是迄今该组织发行的第18篇草药专论。其他已发行的17篇专论是贯叶金丝桃(St.John's Wort)、山楂花叶(Hawthron Leaf with Flower)、黄芪根(Astragalus Root)、山楂果实(Hawthorn     

李红教授治疗亚急性甲状腺炎验案1则

亚急性甲状腺炎(Subacute thyroiditis,SAT)又称病毒性甲状腺炎,De Quervain甲状腺炎等,目前西医尚无有效的治疗方法,李教授运用中药治疗本病取得了较好的治疗效果。     

浅谈亚急性甲状腺炎的中医辨证论治

亚急性甲状腺炎又称肉芽肿性甲状腺炎、巨细胞性甲状腺炎和De Quervain甲状腺炎,本病与病毒感染有关,一般不遗留甲状腺功能减退,以40～ 50岁女性最为多见.