急性肝损伤时F蛋白含量及HGF和挽肝二号的护肝作用

应用Ｄ－氨基半乳糖（Ｄ－ＧＬＡＮ）诱发大白鼠急性肝损伤模型，检测了实验动物的血清Ｆ蛋白含量（ＦＰ）、血清总胆红素（ＳＢ）、凝血酶原时间（ＰＴ）、转氨酶（ＡＬＴ）的水平，观察了急性肝损伤的表现并与促肝细胞生长素（ＨＧＦ）和中药挽肝二号方保护组检测结果相比较。４５只大鼠等分为Ａ、Ｂ、Ｃ３个组。Ａ组由１５只急性肝损伤模型鼠组成，Ｂ组注射Ｄ－氨基半乳糖前先向腹腔注射２ｍｌ ＨＧＦ，Ｃ组注射Ｄ－氨基半乳糖产     

硫普罗宁对急性肝损害保护作用的实验研究

为了研究硫普罗宁（Ｔｉｏｐｒｏｎｉｎ） 对急性肝损害是否有保护作用, 应用Ｄ－氨基半乳糖（Ｄ－ＧａｌＮ） 造成大鼠急性肝损伤模型。实验分成正常组（Ｎ）、模型组（Ｄ－ＧａｌＮ）、模型＋ 硫普罗宁组（Ｄ－ＧａｌＮ＋ Ｔ）、模型＋ 肝得健组（Ｄ－ＧａｌＮ＋ Ｅ）。观察血清中丙氨酸转氨酶（ＡＬＴ）、肿瘤坏死因子（ＴＮＦ－α）、肝细胞及血浆中脂质过氧化物（ＬＰＯ） 和肝组织及红细胞超氧化物歧化酶（ＳＯＤ） 以及相关的病理变化。结果表明： 模型＋ 硫普罗宁组（Ｄ－ＧａｌＮ＋ Ｔ） 及模型＋ 肝得健组（Ｄ－ＧａｌＮ＋ Ｅ） 血清中ＡＬＴ、ＴＮＦ－α及ＬＰＯ与模型组相比明显下降（Ｐ＜ ０．０１）, 而Ｄ－ＧａｌＮ＋ Ｔ和Ｄ－ＧａｌＮ＋ Ｅ两组的ＳＯＤ活性明显高于模型组（Ｄ－ＧａｌＮ） （Ｐ＜ ０．０１）。提示： 硫普罗宁能降低血清中ＡＬＴ、ＴＮＦ－α的含量及肝组织中氧自由基的产生, 降低血浆和组织中ＬＰＯ水平, 并能提高细胞ＳＯＤ活性, 从而减轻肝损伤。其对肝细胞的保护作用与肝得健一致。     

转化生长因子α和增殖细胞核抗原在原发性肝癌及癌旁肝组织中的表达

目的：探讨转化生长因子α（ＴＧＦα）和增殖细胞核抗原（ＰＣＮＡ） 在原发性肝癌（ＨＣＣ） 和癌旁肝组织的表达情况及其与肝组织增殖的关系。方法：用免疫组织化学方法检测ＴＧＦα和ＰＣＮＡ 在正常肝组织、原发性肝癌（ＨＣＣ）和癌旁肝组织的表达。结果：ＴＧＦα和ＰＣＮＡ在正常肝组织中均不表达;６１－０％ 的ＨＣＣ 癌组织和５７－５％ 的癌旁肝组织表达ＴＧＦα;１００ ％ 的癌组织和９７－５ ％ 的癌旁肝组织可观察到ＰＣＮＡ阳性反应。经统计学分析,癌组织的ＴＧＦα染色强度显著高于癌旁肝组织（Ｐ＜０－０１） ;ＴＧＦα阳性组癌组织和癌旁肝组织的ＰＣＮＡ 标记指数（ＬＩ） 均分别高于ＴＧＦα阴性组（ Ｐ均＜０－０１）。结论：ＨＣＣ癌组织和癌旁肝组织存在ＴＧＦα的异常表达;ＴＧＦα与ＨＣＣ癌组织和癌旁肝细胞的增殖密切相关。推测ＨＣＣ中可能存在正反馈的ＴＧＦα自分泌机制     

树Qu实验性肝癌过程中GGT酶阳性肝细胞灶,IGF—Ⅱ及HBxAg异常表达

动态观察ＨＢＶ与ＡＦＢ１诱发树Ｑｕ肝细胞癌（ＨＣＣ）病变过程中有病变γ－谷氨酰转肽酶阳性肝细胞灶ＧＧＴ阳性灶）、胰岛性样生长因子Ⅱ（ＩＧＦ－Ⅱ）发生情况，探讨ＨＣＣ发生的可能机制。实验动物分４组，Ａ组：ＨＢＶ阳性和摄入ＡＦＢ１；Ｂ组：ＨＢＶ阳性组；Ｃ组：摄入ＡＦＢ１；Ｄ组：空白对照。组织化学及免疫组织化学检测肝活检组织，结果：（１）肝癌前病变ＧＧＴ阳性灶在呼实验组不仅灶数量，而且灶大小均明显高于窑     

新生牛肝细胞生长因子的活性研究──Ⅱ．新生牛肝细胞生长因子对大鼠急性肝损伤的保护作用

研究了新生牛肝细胞生长因子（ｃＨＧＦ）对ＣＣｌ４引起的大鼠急性肝损伤的保护作用。结果表明，ｃＨＧＦ可降低ＣＣｌ４引起的急性肝衰竭大鼠的死亡率，抑制血清谷丙转氨酶（ＧＰＴ）和胆红素（Ｂｉｌ）的升高。从肝组织切片观察到ｃＨＧＦ可明显减轻ＣＣｌ＿４引起的肝细胞结构的损伤，证实ＨＧＦ是一种有效的保肝活性因子。     

树鼩实验性肝癌过程中GGT酶阳性肝细胞灶、IGF-Ⅱ及HBxAg异常表达

动态观察ＨＢＶ与ＡＦＢ１诱发树肝细胞癌（ＨＣＣ）病变过程中肝癌前病变γ－谷氨酰转肽酶阳性肝细胞灶（ＧＧＴ阳性灶）、胰岛素样生长因子Ⅱ（ⅠＧＦ－Ⅱ）发生情况，探讨ＨＣＣ发生的可能机制。实验动物分４组，Ａ组：ＨＢＶ阳性和摄入ＡＦＢ１；Ｂ组：ＨＢＶ阳性组；Ｃ组：摄入ＡＦＢ１；Ｄ组：空白对照。组织化学及免疫组织化学检测肝活检组织。结果：（１）肝癌前病变ＧＧＴ阳性灶在各实验组不仅灶数量，而且灶大小均明显高于空白组（Ｐ＜０．０５），且双诱癌因素组（Ａ）灶数量明显大于单诱癌因素组（Ｂ、Ｃ）（Ｐ＜０．０５）；（２）实验第１５周，即有ＩＧＦ－Ⅱ阳性表达，在第４５周阳性率明显高于第７５周（Ｐ＜０．０５）；（３）ＨＢＶ感染组（Ａ，Ｂ）动物肝组织中ＨＢｓＡｇ阳性率高达９２．１％，首次在树肝组织中检出ＨＢｘＡｇ，且阳性率达８６．５％。提示：ＨＢＶ与ＡＦＢ１协同致树ＨＣＣ；ＨＣＣ发生早期就有ⅠＧＦ－Ⅱ异常表达。     

重组人肝再生增强因子对大鼠实验性肝纤维化的保护作用

初步研究表明，从哺乳动物幼仔肝、胚胎肝组织提取的肝细胞生长因子（ＨＧＦ）、肝刺激物（ＨＳＳ）有抗慢性肝损伤的作用。我们以四氯化碳（ＣＣｌ４）中毒性大鼠肝纤维化为模型，研究了重组人肝再生增强因子（ｈＡＬＲ）的抗慢性肝损伤作用，结果表明，重组ｈＡＬＲ有明...     

急性肝损伤大鼠血浆肿瘤坏死因子含量变化研究

本实验应用Ｄ－氨基半乳糖（Ｄ－Ｇｌａｎ）诱发大白鼠急性肝损伤模型，检测了实验动物的血浆肿瘤坏死因子（ＴＮＦ）的含量、血清总胆红素（ＴＢ）和丙氨酸氨基转移酶（ＡＬＴ）水平，观察了急性肝损伤表现并与促肝细胞生长素（ＨＧＦ）和中药保护组（即造模前应用ＨＧＦ组和中药挽肝一号组）相比较。结果：实验肝损伤动物血浆ＴＮＦ含量和其他两项指标均明显升高，在用药（注射Ｄ－Ｇｌａｎ）后２４小时开始升高，４８小时达高峰；     

大鼠急性肝损伤血清TNF、SOD的动态变化及猪再生肝肝细胞生长因子对其的影响

我们采用肝毒性物质ＴＡＡ诱导大鼠急性肝损伤，研究了损伤后血清肿瘤坏死因子（ＴＮＦ）含量、超氧化物歧化酶（ＳＯＤ）活性的动态变化及猪再生肝肝细胞生长因子（ＰＲＨＧＦ）对肝脏的影响，以进一步探讨ＴＮＦ介导肝损伤的机制以及ＰＲＨＧＦ对肝损伤的保护机理，现报...     

清肝解毒注射液治疗大鼠实验性急性肝衰竭的研究

目的：研究清肝解毒注射液治疗大鼠急性肝衰竭（ＦＨＦ）的作用。方法：用Ｄ氨基半乳糖（Ｇａｌｎ）造成大鼠实验性ＦＨＦ，观察清肝解毒注射液对其存活率和肝功能的影响，并设肝细胞生长因子（ＨＧＦ）为对照。结果：清肝解毒注射液组（简称清肝组）大鼠存活率为６３．６４％，ＨＧＦ组和模型组分别为５９．０９％和２７．２７％，清肝组存活率明显高于模型组，Ｐ＜０．０５。该药还可降低ＦＨＦ大鼠ＡＬＴ、ＴＢｉｌ水平，提高ＴＣＨ活性，与模型组比较差异显著。结论：清肝解毒注射液对Ｇａｌｎ所致大鼠实验性ＦＨＦ有明显对抗作用     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.