交链孢酚单甲醚对食管癌旁上皮组织脂质过氧化的影响

用从林县粮食中分离的互隔交链孢霉提取材交链孢酚单甲醚(AME),对22例培养的食管癌患者的癌旁上皮组织进行了攻击。并用抗氧化剂维生素E做了抑制实验。结果发现:(1) AME可以使食管癌旁上皮组织的丙二醛含量升高,与对照组相比较有明显的统计学差异(P<0.01)。在实验浓度范围5～50μg/ml内,存在剂量效应关系(r=0.9740,P<0.025)。(2) 每毫升培养基加入25μg的AME一个小时后,丙二醛含量就升高,八个小时达到高峰,与对照组相比较有明显的差异(P<0.01)。(3) 加入抗氧化剂维生素E后,可以降低AME所诱发的组织中的丙二醛含量,实验组和对照组之间存在着明显的统计学差异(P<0.01)。实验结果说明:AME可以引发食管癌旁上皮组织的脂质过氧化,使丙二醛含量升高,维生素E可以降低这种变化。     

凌霄花抗羊水致大鼠DIC及脂质过氧化作用

本文采用新鲜胎儿羊水舌下静脉注射致大鼠DIC模型,观察血清脂质过氧化产物丙二醛(MDA)含量及抗氧化酶超氧化物歧化酶(SOD)活力的改变与中药凌霄花注射液抗脂质过氧化作用。结果表明,模型组MDA含     

脂质过氧化对器官培养动脉内皮细胞的损伤及抗氧化药物的保护作用

本实验建立了稳定的动脉器官培养方法并以联胺作用于培养的动脉引发脂质过氧化反应,系统地观察硒、维生素E及人参皂甙对内皮细胞脂质过氧化损伤的保护作用。结果表明:联胺激发和促进了脂质过氧化反应引起脂质过氧化物含量增加,内皮细胞生物膜系统损伤及PGI_2合成功能降低。而预先用200 ppb硒、50μg/ml维生索E、10μg/ml人参皂甙作用于动脉,可抑制联胺引起的组织脂贡过氧化物含量升高,使内皮细胞生物膜系统损伤程度减轻并增加PGI_2合成能力。表明硒、维生素E、人参皂甙在一定程度上对内皮细胞过氧化损伤有保护作用,可能对动脉粥样硬化有防治作用。这为抗氧化剂特别是人参皂甙作为抗氧化药物在临床上合理应用提供一定的理论依据。     

家兔多器官衰竭发病过程中脂质过氧化损伤及维生素E对其影响

本文利用家兔多器官衰竭(MOF)模型观察了血丙二醛(MDA)、SOD、过氧化氢酶(AT)及硒谷胱甘肽过氧化物酶(Se-GRH-PX)浓度变化及应用V_E后对其影响,旨在探讨脂质过氧化损伤在MOF发病过程中的作用及早期应用V_E对防治MOF的可能性。     

亚硒酸钠对交链孢酚诱发DNA单链断裂和脂质过氧化的影响

本文采用碱性洗脱-DNA荧光分析法及硫代巴比妥酸反应法,研究了亚硒酸钠(Na_2SeO_3)对交链孢酚(alternariol,AOH)诱发DNA单链断裂和脂质过氧化的影响。结果表明,在人胚食管上皮组织或人胚肺2BS细胞株接触AOH前4小时,在培养基内加10~(-5)或10~(-6)M Na_2SeO_3能显著抑制AOH诱导的DNA单链断裂和脂质过氧化反应;Na_2SeO_3与AOH同时处理对AOH的上述诱变性作用无显著抑制作用,10~(-4)MNa_2ScO_3可诱发人胚肺2BS细胞株DNA单链断裂。结果提示硒能抑制AOH的诱变性,但高浓度硒有毒性作用。     

自由基对培养的鼠肾小球系膜细胞膜系统损伤的研究

目的　观察自由基对肾小球系膜细胞 (GMC)膜系统的影响。方法　用黄嘌呤氧化 酶黄嘌呤生成自由基 ,作用于培养的鼠GMC ,通过TBA比色法检测脂质过氧化产物丙二醛 (MDA)含量及透射电镜观察超氧化物歧化酶 (SOD)对细胞膜系统的保护作用。结果　未加SOD组GMC的MDA含量明显增加 ,电镜下出现细胞膜系统的损伤性改变。加SOD组MDA含量未见明显增加。电镜下细胞膜系统未见明显改变。结论　自由基可引起GMC膜系统脂质过氧化损伤 ,SOD具有保护作用     

小鼠肝LPO、蛋白质含量、SOD活性与实验性高脂血症及年龄的关系

高脂血症是导致动脉粥样硬化(As)主要危险因子,As属老年性疾病,其确切机理尚未阐明,故本文对9、2月龄小鼠同时造成高脂血症模型,观察9、2月龄小鼠形成高脂血症的程度差异,与抗脂质过氧化的中心器官肝脏组织LPO含量和SOD活性及蛋白质     

多棘海盘车AST抗氧化作用研究

目的 以兔和大鼠心肌及肝组织匀浆为材料,研究一种从多棘海盘车(Asteriasamurensis)中提取得到的活性物质经结构改造和修饰得到的丁二酸二-（3β-羟基雄甾5-烯-17-酮）酯新化合物（代号AST）抗脂质过氧化作用.方法 在体外组织匀浆自发或Cys+FeSO4激发脂质过氧化模型上,以丙二醛（MDA）产生作为过氧化程度指标.结果 AST在体外能抑制正常大鼠和兔心肌及肝匀浆过氧化脂质生成,并能对抗半胱氨酸和硫酸亚铁所致过氧化脂质生成增加.结论 AST对兔和大鼠的心肌及肝组织匀浆均有不同程度的抗氧化作用.     

脂质的过氧化反应及Tinoridine对它的抑制作用Ⅱ.抗坏血酸诱导的大鼠肝线粒体脂质的过氧化反应

Tinorldine(一种非固醇类的抗炎药物)对于由抗坏血酸或NADPH诱导的大鼠肝微粒体的脂类过氧化反应有很强的抑制作用,同时可防止细胞色素P-450及膜结构的破坏。线粒体是一种富有多不饱和脂酸的膜性细胞器,而且线粒体的正常功能依赖于膜结构的高度完整性;因此线粒体是很容易受到脂质过氧化反应的影响。已经证明,亚铁离子,抗坏血酸,谷胱甘肽或NADPH诱导的过氧化反应的过程中,线粒体遭到溶解及功能破坏。本文叙述Tinoridine对抗坏血酸诱导的鼠肝线粒体脂质过氧化反应的作用,并从形成的丙二醛的磷脂含量之间的量的关系研究脂质过氧化反应的     

家兔急性感染时血浆过氧化脂质含量与多器官损害的关系

本实验以1次静脉注射大肠杆菌内毒素致家兔急性感染,观察血浆过氧化脂质代谢产物丙二醛(MDA)含量与多器官损害指标BuN,G.P.T,CPK-MB,CPK-BB之间的关系。18只家兔,戊巴比妥钠静脉注射麻醉分二组;Ⅰ组:15只,静注内毒素2mg/kg。二组:3只,静注等量生理盐水作正常对照。注射前及注射后间隔60分钟     

Morphological and biochemical study of the lipid components of the fetal aorta]

We made a biochemical and histochemical study of the lipidic component of intima of fetal aortas on 8 autopsy cases (7 +/- 2 months aged) arrived at our observation in the Pathology's Institute of II Faculty of Naples. We made a study with freeze-sections stained with Oil-Red 0 and after dissociation of the intima by the adventitia, it is valued biochemically the lipidic peroxidation studying the levels of malonyldialdehyde (MDA) like indirect marker of peroxidation. It is known that is present a lipidic component in the intima of fetal aorta whether intracellular or extracellular (Fig. 1, 2). Sometimes this component can accumulate until to determinate true lipidic striae. The aim of this study is a detection of MDA in lipids extracted from human fetal aortas. MDA levels was measured by Thiobarbituric method (TBA): lipids were extracted both intima and adventitia by Chloroform/methanol method, after surgery immediately. The results are expressed in nMoles/mg of lipids +/- Standard Deviation. Controls of spontaneous lipid peroxidation was take at a different times. It is known that in vitro incubation of LDL with cultured endothelial cells, smooth muscle cells or macrophages leads to peroxidation of LDL phospholipids and oxidatively modified LDL become atherogenic via foam cells production. In addition lipid peroxidation was formed by the direct peroxidation of unsaturated fatty acids and their esters are capable of further lipoperoxide production by oxygen free radical; chain reactions. In this context lipid peroxidation could be an important factor in the first stage of human pathophysiological development and this phenomenon may be related by an early free radical production.     

Influence of polychemotherapy on the antioxidant levels and lipid peroxidation in patients with lymphoproliferative diseases

The purpose of the present study was to compare the oxidative stress parameters such as the extent of lipid peroxidation and the status of antioxidants in patients with lymphoproliferative diseases. The effect of polychemotherapy on the level of lipid peroxidation products and on the activities of antioxidant defense enzymes was also investigated. Lipid peroxidation (malondialdehyde or MDA), superoxide dismutase (SOD), catalase (CAT), and ceruloplasmin (CER) activities, were measured in 58 patients with non-Hodgkins lymphoma, Hodgkins disease, chronic lymphocytic leukemia, and acute lymphoblastic leukemia before treatment, during and after polychemotherapy. Plasma levels of MDA and CER in plasma were increased in comparison with the control group before any treatment. Erythrocyte SOD activity was significantly lower but CAT activity was significantly higher in patients than those in controls. Moreover, the levels of MDA and CER increased by the effect of the different regimens of polychemotherapy, which appeared to be compromised by augmented activity of antioxidant enzymes SOD and CAT. The levels of plasma MDA were found to be significantly higher in comparison to the control group for patients treated with cyclophosphamide, vincristine, prednisolon (CVP) and adriamicine, bleomicine, vinblastine, dacarbasine (ABVD), (p<0.01). Erythrocyte SOD statistically decreased (p<0.001) and CAT activities statistically increased in patients treated with CVP and ABVD (p<0.001). In conclusion, our results suggest that after polychemotherapy, the oxidative stress and the imbalance of antioxidant enzyme systems significantly progresses in patients with lymphoproliferative hematological diseases. Patients treated with CVP and ABVD are at the biggest risk of oxidative injury. Thus, the antioxidant status of cancer patients may play an important role in their response to chemotherapy.     

急性脑梗死患者血浆ET、Hcy水平与脂质过氧化的关系

目的：探讨了急性脑梗死患者血浆ET、Hcy水平与脂质过氧化的关系。方法：应用放免法和化学比色法对36例急性脑梗死患者进行了血浆ET、Hcy、SOD、MDA水平检测,并与35名正常健康人作比较。结果：急性脑梗死患者血浆ET、Hcy、SOD、MDA水平非常显著的地高于正常人组（P〈0.01）,而SOD水平则显著的低于正常人组（P〈0.01）,相关分析显示,血象ET、Hcy水平与MDA正相关（r=0.6018、0.6128,P〈0.01）,而SOD与MDA呈负相关（r=-0.4418,P〈0.05）。结论：急性脑梗死患者存在脂质过氧化,与ET、Hcy存在相关性。     

In situ detection of lipid peroxidation in chronic hepatitis C: correlation with pathological features.

AIMS: To assess the occurrence of lipid peroxidation in chronic hepatitis C and to evaluate its relation to pathological features and liver iron concentrations. METHODS: Liver biopsy samples of 43 patients with untreated chronic hepatitis C were studied by immunohistochemistry using specific antibodies directed against two major aldehyde metabolites of lipid peroxidation, malondialdehyde (MDA), and 4-hydroxynonenal (HNE). RESULTS: MDA and HNE adducts (aldehydes covalently linked to another molecule) were detected in the liver samples in 77% and 30% of cases, respectively. MDA adducts were detected both in the extracellular matrix and sinusoidal cells localised in areas of periportal and lobular necrosis. HNE adducts appeared in the cytoplasm of only a few hepatocytes. Comparison of the semiquantitative assessment of adducts (MDA and HNE indexes) with the grading and the staging of chronic hepatitis showed that the MDA index was correlated with fibrosis score (p < 0.001) and the grade of activity (p < 0.01). There was also a tendency to correlation with liver iron concentration (p = 0.09). No correlation was observed between the HNE index and pathological features or liver iron concentration. CONCLUSION: Lipid peroxidation products are detectable in the liver of chronic hepatitis C patients. The presence of MDA adducts in areas of active fibrogenesis and the correlation between the MDA index and fibrosis score suggest a role for lipid peroxidation in liver fibrosis.     

Lipid peroxidation levels in patients with acute brucellosis

The purpose of this study was to investigate levels of lipid peroxidation, indicated by plasma malondialdehyde (MDA), with consideration of clinical status and treatment outcomes in patients with acute brucellosis. Plasma MDA levels were measured in patients with acute brucellosis and healthy subjects. Significantly higher MDA levels were detected in plasma of patients with acute brucellosis compared to controls (P<0.01). Plasma levels of MDA were significantly decreased after the brucellosis treatment (P<0.01). The results of the present study indicate for the first time that a considerable level of lipid peroxidation is involved in acute brucellosis cases and this may be of importance with respect to the understanding of disease pathogenesis and may serve as a target for treatment regime.     

Increased urinary lipoperoxide levels in renal transplant patients

Using high performance liquid chromatographic methods, both plasma and urine lipoperoxide concentrations were measured, as malondialdehyde (MDA), in 30 stable renal transplant patients receiving daily cyclosporine and/or azathioprine therapy. Their MDA concentrations were compared with previously reported reliable reference values using the same liquid chromatographic methods. Although their plasma concentrations were within the reference range, their mean urine MDA values averaged 3.7 to 5.0 times the normal reference values (p less than 0.001). The primary cause of the increased urine MDA concentrations following renal transplantation in these patients is unknown; it could be due to (a) renal lipid peroxidation directly related to the cyclosporine/azathioprine therapy, (b) drug-induced or other nephrotoxicity by an alternative mechanism with secondary lipid peroxidation, (c) increased lipid peroxidation owing to an immunologic response to the kidney graft, or (d) a combination of these possibilities.     

The effect of metal chelators on lipid peroxidation in stored erythrocytes.

Lipid peroxidation, an autocatalytic free radical process, is increased in a wide variety of conditions including various hemolytic anemias and hemoglobin disorders. Increased erythrocyte lipid peroxidation occurs in the presence of iron species and some heme moieties. In this study, greatly reduced concentrations of malondialdehyde (MDA), an indicator of lipid peroxidation, were observed in stored blood anticoagulated with citrate, phosphate, dextrose, and adenine (CPDA-1) upon the addition of deferoxamine mesylate (DM), diethylenetriaminepentaacetic acid (DTPA), and ethylenediaminetetraacetic acid (EDTA) (P less than 0.001). Human transferrin has essentially no effect on lipid peroxidation. Conversely, penicillamine and butylated hydroxytoluene significantly increased lipoperoxide production. Although bathophenanthroline disulfonic acid (BPD) appeared to be very effective in reducing lipid peroxidation, its complex chromatographic patterns which were due to the presence of multiple thiobarbituric acid reactive substances, were difficult to fully evaluate. The addition of appropriate metal chelators to stored blood may be effective in increasing the viability and longevity of transfused red cells.     

Molecular mechanisms of cataractogenesis: IV. Evidence of phospholipid . malondialdehyde adduct in human senile cataract

Lipid extracts of the human cataractous and normal lenses were analyzed by thin-layer chromatography (TLC) using a solvent system consisting of CHCl3/CH3OH/CH3COOH/H2O (50:25:7:3 by vol.). A novel phospholipid having a Rf intermediate between phosphatidyl ethanolamine (PE) and phosphatidyl serine (PS) was detected besides the four major phospholipids viz., PE, PS, phosphatidyl choline (PC) and sphingomyelin (SP). The novel phospholipid was found to be molybdenum positive and ninhydrin negative having a characteristic fluorescence of Schiff-base conjugate formed between PE, malondialdehyde (MDA) and PS. It was possible to resolve this adduct into PE and PS after acid hydrolysis using two dimensional TLC with CHCl3/CH3OH/NH3 (7 M) (65:25:4 by vol.) as the second solvent. In cataract PE . MDA . PS adduct increased significantly as did diene conjugates and MDA. In plasma membrane lipid extract of cataractous lenses there was a marked increase in fluorescence at 460 nm when excited at 365 nm showing a characteristic fluorescence of a typical Schiff-base conjugate. The evidence suggests that peroxidation of lenticular plasma membrane lipids is one of the molecular mechanisms involved in cataract in the human.     

Lipid peroxidation levels in patients with acute brucellosis

Abstract The purpose of this study was to investigate levels of lipid peroxidation, indicated by plasma malondialdehyde (MDA), with consideration of clinical status and treatment outcomes in patients with acute brucellosis. Plasma MDA levels were measured in patients with acute brucellosis and healthy subjects. Significantly higher MDA levels were detected in plasma of patients with acute brucellosis compared to controls (P<0.01). Plasma levels of MDA were significantly decreased after the brucellosis treatment (P<0.01). The results of the present study indicate for the first time that a considerable level of lipid peroxidation is involved in acute brucellosis cases and this may be of importance with respect to the understanding of disease pathogenesis and may serve as a target for treatment regime.