他莫西芬对去势大鼠骨生物力学性能的影响

目的 评价他莫西芬对雌激素缺乏引起的骨生物力学性能下降的预防作用。方法 采用4月龄Sprague-Dawley(SD)雌性大鼠切除卵巢作动物模型，术后给予他莫西芬治疗连续12周。结果 与假手术组相比去势组骨密度、股骨生物力学性质、腰椎骨形态计量指标均有显差异；他莫西芬处理组与去势组比较，股骨骨密度、股骨最大载荷、桡度、最大应力、弹性模量显升高、骨形态计量参数的改变均有显性。结论 他莫西芬能抑制去势引起的SD大鼠骨生物力学性能下降。     

高原去势大鼠松质骨的微观结构及力学性能变化的研究

目的观察高原低氧环境下骨质疏松大鼠模型的松质骨骨密度、力学性能及骨小梁结构的变化。方法选用纯种SD雌性大白鼠72只,随机分为高原去势组和平原去势组、高原对照组和平原对照组。术后分别在海拔高于3000m的高原和低于500m的平原饲养。并于术后1个月、3个月和6个月测量大鼠的股骨骨密度和腰椎生物力学强度,并行Micro-CT分析。结果 3个月时,高原和平原饲养的去势大鼠骨密度下降较为显著(P<0.05),且高原去势组骨密度明显低于平原去势组(P<0.05),对照组均无显著变化(P>0.05)。6个月时,除平原对照组骨密度无显著变化,其他三组骨密度均有显著降低,且高原去势组骨密度明显低于其他组,差异具有统计学意义(P<0.01)。生物力学检测发现,去势6个月以后,高原去势组、平原去势组、高原对照组和平原对照组抗压强度较去势后1个月分别降低29.48%、24.85%、15.63%和3.98%,高原去势组下降更为明显(P<0.01)。Mi-cro-CT分析显示,高原去势组大鼠骨的相对骨体积(BV/TV)、骨小梁数目(Tb.N)显著低于其他组,骨小梁分离度(Tb.Sp)则较其他组显著增高,差异具有统计学意义(P<0.01)。结论高原缺氧环境可引起骨代谢异常,并加速骨质疏松发展的进程,应用Micro-CT分析有助于研究高原骨质疏松动物模型的骨小梁结构变化。     

卵巢切除大鼠皮质骨骨密度与生物力学特性分析(英文)

目的观察去势后不同时间大鼠皮质骨骨密度与生物力学特性的变化,以探讨雌激素减少对皮质骨骨密度及生物力学特性的影响。方法40只雌性大鼠随机分为去势组和对照组,各组分别于术后6及12周各处死其中10只。取血、尿标本分别测血清Ca,P,碱性磷酸酶(ALP)和尿Ca,P,羟脯氨酸(HOP)。用骨密度仪测定大鼠胫骨骨密度,用三点挤压实验测定股骨最大载荷、弯曲刚度、最大应力以及弹性模量的变化。结果去势6及12周组血Ca,ALP,尿Ca/Cr,尿HOP/Cr均明显高于对照组(P<0.01),而血P、尿P/Cr与对照组相比无显著差异(P>0.5)。去势6周骨密度与最大载荷、弯曲刚度、最大应力以及弹性模量与对照组相比无显著差异(P>0.05),而去势12周组骨密度明显低于对照组(P<0.01),而最大载荷、弯曲刚度、最大应力以及弹性模量均低于对照组(P<0.05)。结论骨密度和生物力学参数的测量对于骨质疏松症动物模型的建立及治疗骨质疏松症药物疗效的判断有重要的意义。     

增龄性变化对大鼠骨质量、血清骨代谢指标影响研究

[目的]探讨增龄性变化对大鼠骨质量、血清骨代谢指标的影响.[方法]14只新生雄性Sprague Dawley(SD)大鼠,同等条件下饲养,饲养至6个月按体重随机分为老龄骨质疏松组(AO组)和青年对照组(YC组),将YC组安乐处死,AO组继续饲养至22个月,两组大鼠均取左侧股骨,测量骨密度、骨组织形态计量学指标,以及骨矿水平及骨代谢指标.[结果] 腰椎及股骨密度(BMD)在老龄大鼠均下降,其中股骨密度下降尤其显著(P<0.01) .与YC组比较,AO组大鼠骨钙素水平显著降低(P<0.01),抗酒石酸酸性磷酸酶(TRACP)水平显著升高(P<0.05);骨生物力学测定发现,AO组大鼠的股骨最大受力负荷下降.骨形态计量学测定发现,AO组大鼠骨小梁体积、骨小梁宽度、骨皮质厚度显著下降(P<0.05或P<0.01),骨小梁间隙增大( P<0.01),AO组大鼠骨小梁普遍变薄、变细甚至断裂穿孔.[结论]22月龄雄性大鼠发生了骨质疏松的变化,骨形成降低,骨矿含量下降,骨微观组织结构破坏.     

骨密度、骨矿含量与椎体生物力学的相关性及中药骨康的干预作用

目的:探讨骨质疏松大鼠骨密度与骨生物力学间的关系及中药骨康的干预作用。方法:通过切除大鼠卵巢造成的骨质疏松模型,随机分A组(正常对照组)、B组(模型组)、C组(中药骨康组)3组,观察骨质疏松大鼠的骨密度、骨矿含量与第3腰椎生物力学的变化及相互之间的关系。结果:去势30d时,B、C2组大鼠骨密度和骨矿含量明显低于A组(P<0．05),但生物力学变化不明显(P>0．05);去势45d时,B、C2组大鼠骨密度和骨矿含量生物力学均明显低于A组(P<0．05或0．01);45d造模成功后,C组给予中药骨康治疗45d后,骨密度和骨矿含量均有明显提高(P<0．05),但椎体生物力学却无明显变化(P>0．05);给予中药骨康治疗90d后,骨密度和骨矿含量均有明显提高(P<0．01),椎体生物力学明显改善(P<0．05)。结论:提示骨质疏松发病时,先有骨的丢失,出现骨密度的下降,然后再有骨生物力学的降低,在骨质疏松的治疗时,骨的生物力学的改善又迟于骨密度的提高;在骨质疏松症的防治时,应注意这些不一致性,以便更好地防止骨质疏松骨折的发生。中药骨康能够提高去势大鼠骨密度,提高其骨生物力学性能。     

增龄性变化对大鼠骨质量、血清骨代谢指标影响研究

[目的]探讨增龄性变化对大鼠骨质量、血清骨代谢指标的影响。[方法]14只新生雄性Sprague Dawley（SD）大鼠，同等条件下饲养，饲养至6个月按体重随机分为老龄骨质疏松组（AO组）和青年对照组（YC组），将YC组安乐处死，AO组继续饲养至22个月，两组大鼠均取左侧股骨，测量骨密度、骨组织形态计量学指标，以及骨矿水平及骨代谢指标。[结果]腰椎及股骨密度（BMD）在老龄大鼠均下降，其中股骨密度下降尤其显著（P〈0.01）。与YC组比较，AO组大鼠骨钙素水平显著降低（P〈0.01），抗酒石酸酸性磷酸酶（TRACP）水平显著升高（P〈0.05）；骨生物力学测定发现，AO组大鼠的股骨最大受力负荷下降。骨形态计量学测定发现，AO组大鼠骨小梁体积、骨小梁宽度、骨皮质厚度显著下降（P〈0.05或P〈0.01），骨小梁间隙增大（P〈0.01），AO组大鼠骨小梁普遍变薄、变细甚至断裂穿孔。[结论]22月龄雄性大鼠发生了骨质疏松的变化，骨形成降低，骨矿含量下降，骨微观组织结构破坏。     

卵巢切除大鼠皮质骨骨密度与生物力学特性分析

目的 观察去势后不同时间大鼠皮质骨骨密度与生物力学特性的变化，以探讨雌激素减少对皮质骨骨密度及生物力学特性的影响。方法 40只雌性大鼠随机分为去势组和对照组，各组分别于术后6及12周各处死其中10只。取血、尿标本分别测血清Ca，P，碱性磷酸酶(ALP)和尿Ca，P，羟脯氨酸(HOP)。用骨密度仪测定大鼠胫骨骨密度，用三点挤压实验测定股骨最大载荷、弯曲刚度、最大应力以及弹性模量的变化。结果 去势6及12周组血Ca，ALP，尿Ca／Cr，尿HOP／Cr均明显高于对照组(P&;lt;0.01)，而血P、尿P／Cr与对照组相比无显著差异(P&;gt;0．5)。去势6周骨密度与最大载荷、弯曲刚度、最大应力以及弹性模量与对照组相比无显著差异(P&;gt;0．05)，而去势12周组骨密度明显低于对照组(P&;lt;0．01)，而最大载荷、弯曲刚度、最大应力以及弹性模量均低于对照组(P&;lt;0．05)。结论 骨密度和生物力学参数的测量对于骨质疏松症动物模型的建立及治疗骨质疏松症药物疗效的判断有重要的意义。     

被动吸烟大鼠骨量与骨生物力学指标的相关性及淫羊藿黄酮的干预效应

目的:作者已通过实验证实,被动吸烟可以增加大鼠骨吸收,最终导致骨质疏松.淫羊藿黄酮有促进骨形成防止骨丢失的作用.观察淫羊藿黄酮干预对被动吸烟大鼠骨密度与骨生物力学指标的影响,并分析骨密度占骨生物力学指标的相关性.方法:实验于2005-01/12在中南大学湘雅医学院附属湘雅医院动物实验室和中心实验室完成,对动物的处置方法符合动物伦理学要求.选择SD大鼠60只,按随机数字表法分成6组(n=10):即空白对照组、被动吸烟组、被动吸烟 钙组、淫羊藿黄酮75,150,300mg/(kg·d)组,后5组大鼠按密室熏烟法被动吸烟4个月,每天灌服钙剂及淫羊藿,给药4个月.干预完毕后测定股骨和腰椎骨密度、L4椎体力学性能指标、股骨结构力学性能指标及材料力学性能指标,并分析骨密度与骨生物力学指标的相关性.结果:60只大鼠全部进入结果分析.①与空白对照组比较,被动吸烟组大鼠的股骨和腰椎骨密度、L4椎体力学性能指标、股骨结构力学性能指标及材料力学性能指标均明显降低(P＜0.05);与被动吸烟组比较,被动吸烟 钙组、淫羊藿黄酮各剂量组上述指标均得到改善(P＜0.05).②股骨和腰椎骨密度分别与腰椎体力学性能指标中弹性模量、最大载荷、最大应力、变形位能、结构刚度,股骨结构力学性能指标中最大载荷、破坏载荷、变形位能、结构刚度和股骨材料力学性能指标中弹性模量、最大应力、最大应变、破坏应力呈正相关(股骨骨密度:r=0.802～0.822,P＜0.05;腰椎骨密度:r=0.784～0.843, P＜0.05).结论:淫羊藿黄酮可改善和提高被动吸烟大鼠骨密度、椎骨力学性能、股骨结构力学性能和材料力学性能,且骨密度与骨生物力学指标之间存在明显的相关性.     

复方脑复康对去卵巢骨质疏松大鼠股骨骨密度及生物力学的影响

目的探讨复方脑复康对去卵巢(OVX)骨质疏松大鼠股骨骨密度及股骨生物力学的影响。方法40只6、7月龄雌性SD大鼠随机分为4组:假手术(Sham)组、OVX组、OVX+己烯雌酚(E)组和OVX+复方脑复康(F)组,每组10只。实行手术后,于术后第3天开始灌胃用药。90天后处死大鼠,取左侧股骨进行股骨骨密度和生物力学的测量。结果与Sham组比,OVX组股骨骨密度及生物力学指标明显下降,全股骨密度、弹性载荷和弯曲能量差异有显著性(P<0.05),而用己烯雌酚和复方脑复康能阻止这种变化;与E组相比,F组的这种阻止作用更为明显。结论复方脑复康能防治OVX造成的骨质疏松,增加骨密度,防止骨生物力学性能的受损,效果优于单独应用己烯雌酚治疗。     

仙珍骨宝干预泼尼松诱发大鼠的骨丢失

背景:仙珍骨宝胶囊由蛇床子、淫羊藿等制成,对糖皮质激素、维甲酸等诱发的骨质疏松有一定的预防作用。目的:建立泼尼松诱发大鼠骨质疏松模型,通过骨形态计量学和骨生物力学等方法探讨仙珍骨宝对大鼠不同部位骨骼的影响。方法:32只SD大鼠随机分成对照组、模型组和仙珍骨宝高、低剂量组。后3组给予泼尼松3.5mg/(kgd)灌胃建立骨质疏松模型。仙珍骨宝高、低剂量组大鼠在泼尼松灌胃1h后给予仙珍骨宝胶囊0.95g/(kgd)、0.475g/(kgd)。结果与结论:与对照组比较,模型组大鼠股骨骨密度、弹性载荷、最大载荷和断裂载荷均下降,胫骨上段动态参数标记周长百分数、骨表面形成率、骨转换率均降低,但静态参数骨小梁面积百分数、骨小梁数量、骨小梁厚度和骨小梁分离度无明显变化。胫骨中段皮质骨形态计量学参数均无明显变化。与模型组比较,仙珍骨宝高剂量组股骨密度、骨生物力学参数,胫骨上段标记周长百分数、骨表面形成率和骨转换率增加,但胫骨中段的骨形态计量学参数无明显变化。与模型组比较,仙珍骨宝低剂量组各项参数均无明显变化。提示高剂量仙珍骨宝促进骨形成,可预防泼尼松所致大鼠股骨骨密度及生物力学的降低,但对胫骨中段皮质骨无明显影响。     

不同强度跑台运动与雌激素联合作用对实验性骨质疏松大鼠骨量的影响

目的 :将成年SD大鼠去卵巢建立骨质疏松模型 ,探讨不同强度运动与雌激素联合作用对骨质疏松大鼠骨量的影响。方法 :实验动物分基础对照组、正常对照组、阳性对照组、雌激素对照组、中等强度运动组、大强度运动组、中等强度运动 雌激素组和大强度运动 雌激素组。用骨形态计量学方法 ,研究了 8周的不同强度跑台运动 ,以及运动和雌激素结合对大鼠胫骨松质骨骨量的影响。结果 :中等强度运动组骨高转换受到抑制 ,骨量丢失减缓。大强度运动仍为高转换型骨代谢 ,且骨量低于中等强度运动组。两个运动加雌激素组明显抑制了去卵巢后的高骨转换 ,骨量较两个单纯运动组和雌激素对照组增多。结论 :雌激素和运动的结合比单纯用药和单纯运动能起到更好的预防骨丢失的作用     

高压氧和静磁对去势雌鼠骨密度的影响

目的 探讨高压氧和静磁对去势雌鼠骨密度（BMD）的影响。方法 5月龄SD大鼠50只,切除双侧卵巢,随机分成模型组、高压氧组、静磁组、高压氧＋静磁组及假手术组（正常对照组）,每组10只;采用QDR4500A型扇形束DEXA检测各组腰椎（L4－6）、股骨、胫骨各区域和全身BMD,并进行分析比较。结果 14周后高压氧组与模型组比较,高压氧组各部位BMD略呈上升趋势,但差异无统计学意义。静磁组与模型组比较,在股骨远端（FR2）BMD明显下降,差异有显著性意义（P＜0.05）。高压氧＋静磁组与高压氧组比较,前者各部位BMD略呈下降趋势;而高压氧＋静磁组与静磁组比较,前者各部位BMD略呈上升趋势;但差异均无显著性意义。结论 高压氧对去势雌鼠骨丢失无明显改善,强磁场加重去势雌鼠的骨丢失,高压氧能部分拮抗强磁场所致的去势雌鼠的骨丢失。     

Dose effects of propranolol on cancellous and cortical bone in ovariectomized adult rats

Animal studies suggest that bone remodeling is under beta-adrenergic control via the sympathetic nervous system. The purpose of this study was to examine the preventive effect of different doses of nonspecific beta-blockers (propranolol) on trabecular and cortical bone envelopes in ovariectomized rats. Six-month-old female Wistar rats were ovariectomized (OVX, n = 60) or sham-operated (n = 15). Then, OVX rats were subcutaneously injected with 0.1 (n = 15), 5 (n = 15), or 20 (n = 15) mg/kg propranolol or vehicle (n = 15) for 10 weeks. Tibial and femoral bone mineral density (BMD) were analyzed longitudinally by dual-energy X-ray absorptiometry. At death, the left tibial metaphysis and L(4) vertebrae were removed, and microcomputed tomography (Skyscan 1072; Skyscan, Aartselaar, Belgium) was performed for trabecular bone structure investigation. Histomorphometry analysis was performed on the right proximal tibia to assess bone cell activities. After 10 weeks, OVX rats had decreased BMD and trabecular parameters and increased bone turnover, as well as cortical porosity compared with the sham group (p < 0.001). Bone architecture alteration was preserved by 0.1 mg/kg propranolol due to higher trabecular number and thickness (+50.35 and +6.81%, respectively, than OVX; p < 0.001) and lower cortical pore number (-52.38% than OVX; p < 0.001). Animals treated by 0.1 mg/kg propranolol had a lower osteoclast surface and a higher osteoblast activity compared with OVX. Animals treated by 20 mg of propranolol did not significantly differ from OVX rats. Animals treated by 5 mg of propranolol have been partially preserved from the ovariectomy. These results showed a dose effect of beta-blockers. The lower the dose of propranolol breeding, the better the preventive effect against ovariectomy.     

脊髓损伤对大鼠股骨骨密度与生物力学特性的影响

目的探讨脊髓损伤(spinal cord injury, SCI)对大鼠股骨生物力学和骨密度(bone mineral density, BMD)的影响. 方法将40只3月龄大鼠随机分为SCI组和对照组,SCI组于T10椎体处完全切断脊髓,对照组仅行椎板切除术.于术后3,6周分2批处死动物进行股骨骨密度和生物力学测定. 结果术后3周时SCI组大鼠股骨远端BMD较对照组显著下降(P<0.05);术后6周时SCI组股骨近端BMD与对照组比较,下降差异显著(P<0.05);股骨远端BMD较对照组下降差异非常显著(P<0.01);股骨干BMD与对照组相比有降低趋势,但无统计学意义.术后3周时股骨颈最大载荷、最大变形、结构刚度及能量吸收在2组间差异均无统计学意义.术后6周时最大变形、结构刚度和能量吸收均低于对照组(P均<0.05),最大载荷低于对照组,差异具有极显著性意义(P<0.01). 结论脊髓损伤后松质骨骨密度和生物力学性能的降低先于密质骨.脊髓损伤后6周时SCI组股骨松质骨的骨密度及生物力学参数均显著低于对照组,脊髓损伤后6周的大鼠可作为骨质疏松动物模型.     

脉冲电磁场及阿仑膦酸钠对脊髓损伤大鼠骨质量的影响

目的探讨早期应用脉冲电磁场（PEMFs）和／或阿仑膦酸钠（ALN）对脊髓损伤（SCI）后大鼠骨质量的影响。方法将62只3月龄雌性sD大鼠随机分为5组，其中假手术组仅行椎板切除术，SCI组、SCI＋ALN组、SCI＋PEMFs组及SCI＋PEMFs＋ALN组行脊髓完全横断术，后3组于术后1周时分别给予ALN和／或PEMFs治疗；于术后8周时取材，检测各组大鼠股骨骨密度值、生物力学指标以及胫骨近端骨组织形态计量学等指标。实验所得数据比较采用单因素方差分析及析因分析。结果早期应用ALN和／或PEMFs均可使股骨骨密度、股骨弹性载荷、最大载荷等生物力学指标增高，胫骨近端骨小梁面积百分比也同时增大，骨小梁宽度增加。结论早期应用ALN和／或PEMFs均可抑制SCI后骨量丢失，增强抗骨折能力，阻止胫骨近端微观结构的退变，提示早期应用ALN及PEMFs有助于防治SCI后骨质疏松，但其具体治疗机制还有待进一步研究。     

Comparison of calcitonin, alendronate and fluorophosphate effects on ovariectomized rat bone.

The effects of calcitonin, alendronate and fluorophosphate preventive treatment on ovariectomized rat femur were studied by comparing densitometric, mechanical, mineralogical and histomorphometric data. Sixty retired breeder female Sprague-Dawley rats, aged 10 months, were randomly divided into six groups. A group (baseline) was euthanized at the beginning of the study as a baseline group; four groups were ovariectomized and one was sham-operated (sham) and considered as a sham-aged group. A group of ovariectomized rats was used as a sham-therapy control (OVX) and received only deionized drinking water, while the other three received: a) salmon calcitonin (SCN) at a dose of 2 IU/kg/d s.c. (OVX + SCN); b) alendronate sodium salt (ALN) at a dose of 6 microg/kg/d administered by gavage (OVX + ALN); and c) L-glutamine monofluorophosphate (G-MFP) and calcium at a rate of 1:30 F/Ca at a dose of 0.21 mg F/6.30 mg Ca per kg/d by gavage (OVX + MFP). Significant increases (P < 0.05) of about 15 and 27% in femoral proximal epiphysis bone mineral density (BMD) of the OVX + ALN group were observed versus healthy groups and the OVX group, respectively. The OVX + ALN group also showed significant increases in femoral mid-diaphysis BMD when compared to OVX (18%, P < 0.001), OVX + SCN (14%, P < 0.05) and OVX + MFP (18%, P < 0.001) groups. In the OVX + MFP group, the three-point bending test demonstrated significant increases (P < 0.05) in maximal load of 21 and 22% when compared to the OVX and OVX + SCN groups, respectively. Also, stiffness data showed significant increases of the OVX + MFP (17%) and sham (14%) groups in comparison with the OVX group. A decrease in Mg (42%, P < 0.05), and increases in Ca (15%, P < 0.0001) and PO4 (8%, P < 0.005) content were found by comparing OVX + MFP and OVX groups. Trabecular bone volume results showed significant increases by comparing OVX + ALN and OVX groups (12.20%, P < 0.0005), as well as control groups. Tested agents were able to reduce the bone loss due to estrogen deficiency, but this did not always produce an increase in strength of the treated bone. Alendronate treatment prevented a decrease in bone mineral density and maintained bone mechanical properties after ovariectomy without impairment of bone mineralization in aged rats.     

去卵巢大鼠骨密度变化与骨髓造血功能改变的关系

本研究探讨去卵巢大鼠不同时段骨密度的改变与骨髓造血功能改变的关系。探讨骨质疏松的发生与造血功能的关联。50只3月龄SD雌性大鼠随机分为去卵巢（OVX）组和假手术（Sham）组,分别在4周、8周、12周、16周和20周处死。取左侧股骨测量骨密度。右侧远端股骨干骺端松质骨甲醛固定,常规制片观察骨髓组织病理改变,集落形成实验观察造血干细胞集落形成能力,酶联免疫吸附试验（ELISA）检测血清造血生成因子粒-巨噬细胞集落刺激因子（GM—CSF）的浓度。结果表明,大鼠去卵巢4周还未出现明显的骨量丢失时,其骨髓组织即出现明显的脂肪组织容量增加,大鼠去卵巢8周、12周、16周和20周,与同时段假手术组比较股骨骨密度显著减低（P〈0．05）,同时伴有骨髓造血组织容量减少,脂肪组织容量增高,巨核细胞数目减少,破骨细胞数目和肥大细胞数目显著增加,与假手术组比较均有显著性差异（P〈0．05）。大鼠去卵巢16周后,造血干细胞形成粒-巨噬细胞集落能力明显低于假手术组。大鼠去卵巢12周后,GM—CSF水平明显低于假手术各组（P〈0．05）。结论：去卵巢大鼠骨密度减低的同时存在骨髓造血功能的减低,3月龄SD雌性大鼠去卵巢可以作为造血功能减低的动物模型,有助于临床上诸多造血功能减低的研究。     

Effects of single and concurrent intermittent administration of human PTH (1-34) and incadronate on cancellous and cortical bone of femoral neck in ovariectomized rats

The purpose of this study is to determine the efficacy of concurrent treatment with human parathyroid hormone, hPTH (1-34), and bisphosphonate (incadronate) in augmenting cortical and cancellous bone mass of femoral neck in ovariectomized (OVX) rats. Forty-eight 11-week-old female Sprague-Dawley rats were divided into eight groups (six animals in each group). The baseline control group was killed at the beginning of the experiment, at 11 weeks of age. An ovariectomy was performed in thirty rats and twelve rats were subjected to a sham surgery. OVX rats were untreated for the first four weeks of postsurgery to allow for the development of moderate osteopenia. These animals were then subjected to various treatments with either PTH, incadronate, or PTH+ incadronate for a period of 4 weeks. Right proximal femora (femoral necks) were used for bone histomorphometry. After OVX 8 weeks, there was a significant decrease in cancellous bone mass and cortical bone area of femoral neck in the OVX rats when compared to the sham control rats. In OVX rats treated with PTH alone or PTH+ incadronate were completely restored lost cancellous and cortical bone mass of femoral neck by increase bone formation. The bone formation parameters (OS/ BS, MS/BS) and bone turnover (BFR/BV) seen with PTH plus incadronate were similar to those seen with PTH treatment alone. This indicates that incadronate did not blunt the anabolic action of PTH when used concurrently. Our results suggest the followings: 1) the femoral neck of OVX rats is a suitable sample site for preclinical studies of the prevention of bone loss induced by estrogen depletion; 2) concurrent use of incadronate did not blunt the anabolic effect of PTH; 3) concurrent treatment showed the best results in restoring cancellous and cortical bone mass; and 4) it had additional benefits for bone strength independent of that achieved by the increase in bone mass.     

Efficacy of static magnetic field for locomotor activity of experimental osteopenia

In order to examine the effectiveness of applying a static magneticfield (SMF) for increasing bone mineral density (BMD), we assessedthe degree of osteopenia by dual-energy X-ray absorptiometry(DEXA), the metabolism measuring system, and histological examinationof bone tissue in an ovariectomized (OVX) rat model. Thirty-sixfemale Wistar rats (8 weeks old, 160–180 g) were dividedinto three groups. The rats in the OVX-M group were exposedto SMF for 12 weeks after ovariectomy. The ovariectomized ratsin the OVX-D group were not exposed to SMF as a control. Therats in the normal group received neither ovariectomy nor exposureto SMF. Twelve-week exposure to SMF in the OVX-M group inhibitedthe reduction in BMD that was observed in the OVX-D group. Moreover,in the OVX rats, before exposure to SMF, there was no cleardifference in the level of locomotor activity between the activeand resting phases, and the pattern of locomotor activity wasirregular. After exposure of OVX rats to SMF, the pattern oflocomotor activity became diphasic with clear active and restingphases, as was observed in the normal group. In the OVX-M group,the continuity of the trabecular bone was maintained more favorablyand bone mass was higher than the respective parameters in theOVX-D group. These results demonstrate that exposure to SMFincreased the level of locomotor activity in OVX rats, therebyincreasing BMD.