生精冲剂对实验性精索静脉曲张大鼠生精细胞凋亡状况的影响

目的:探讨实验性精索静脉曲张大鼠睾丸生精细胞凋亡状况以及生精冲剂对其凋亡的影响。方法:将60只成年雄性W istar大鼠随机抽出20只为对照组,其余按石津和彦改良法制成左精索静脉曲张大鼠模型,再随机分为模型组20只,治疗组20只。用末端脱氧核苷酸转移酶介导的原位缺口末端标记法(TUNEL)检测睾丸生精细胞凋亡。结果:模型组大鼠睾丸生精细胞凋亡指数明显高于对照组(P<0.01)和治疗组(P<0.01),治疗组与对照组比较有明显差异(P<0.01)。结论:实验性精索静脉曲张大鼠睾丸生精细胞凋亡增加,这可能是影响生育力的机制之一;生精冲剂能够减少精索静脉曲张大鼠睾丸生精细胞凋亡,对睾丸生精功能具有保护作用,进而可提高睾丸的生殖能力。     

茶多酚对精索静脉曲张大鼠睾丸生精细胞凋亡的影响

目的:研究茶多酚对精索静脉曲张大鼠睾丸生精细胞凋亡的影响,分析茶多酚对精索静脉曲张大鼠生精功能是否具有保护作用。方法:清洁级青春期Wistar大鼠32只随机分为4组,每组8只:假手术组(仅模拟手术过程,不结扎血管)、模型组(建立左精索静脉曲张模型)、茶多酚低剂量组[建立精索静脉曲张模型并给予10 mg/(kg·d)茶多酚干预]、茶多酚高剂量组[建立精索静脉曲张模型并给予40 mg/(kg·d)茶多酚干预]。建立左侧精索静脉曲张模型4周后,假手术组及模型组均给予生理盐水1 ml/100 g,茶多酚低剂量组及茶多酚高剂量组分别给予茶多酚10 mg/kg(用生理盐水配置成浓度为1 mg/ml)和40 mg/kg(用生理盐水配置成浓度为4 mg/ml),灌胃,1次/日,持续4周。4周后4组大鼠均处死并分别取左侧睾丸组织,检测低氧诱导因子-1(HIF-1)、Bcl-2、Bax、细胞色素C(Cyt C)和caspase-3在睾丸组织中的表达及生精细胞的凋亡并计算凋亡指数(AI)。结果:茶多酚干预组大鼠Bcl-2表达高于模型组(110.03±3.07),低于假手术组(154.18±2.96);茶多酚低剂量组大鼠Bcl-2(127.67±1.28)表达低于茶多酚高剂量组(136.41±1.95),差异有统计学意义(P0.05)。茶多酚干预组大鼠HIF-1、Bax、Cyt C和caspase-3的表达低于模型组,高于假手术组;茶多酚低剂量组大鼠HIF-1、Bax、Cyt C和caspase-3的表达高于茶多酚高剂量组,差异有统计学意义(P0.05)。假手术组AI最低,茶多酚干预组AI低于模型组,茶多酚高剂量组AI低于茶多酚低剂量组,差异有统计学意义(P0.05)。结论:茶多酚能显著降低精索静脉曲张大鼠睾丸生精细胞的凋亡。     

通精灵对实验性精索静脉曲张大鼠生精细胞凋亡及Bcl-2表达的影响

目的：探讨实验性精索静脉曲张大鼠睾丸生精细胞凋亡状况及通精灵对精索静脉曲张大鼠睾丸生精功能的保护作用。方法：成年清洁级雄性Wistar大鼠50只,按Sapyol法制作左精索静脉曲张大鼠模型,随机分为假手术组、模型组、通精灵低剂量组、通精灵中剂量组、通精灵高剂量组各10只。造模1个月后,各组予以不同药物灌胃1次／d。2个月后处死,用原位末端脱氧核苷酸转移酶标记法检测睾丸生精细胞凋亡,免疫组化法检测睾丸组织Bcl-2表达。结果：模型组大鼠左睾丸凋亡指数明显高于假手术组及通精灵各组（P〈0．01）,通精灵各组左睾丸凋亡指数显著低于模型组（P〈0．05）。与假手术组比较,模型组大鼠睾丸组织Bcl-2蛋白表达下降（P〈0．05）,与模型组比较,通精灵中、高剂量组睾丸组织Bcl-2蛋白表达显著上升（P〈0．05）。结论：通精灵能降低实验性精索静脉曲张大鼠睾丸生精细胞凋亡,减轻实验性精索静脉曲张大鼠睾丸组织的病理损害,上调凋亡抑制基因Bcl-2表达。     

精索静脉曲张大鼠生精细胞凋亡的实验研究

目的 :探讨外科所致的精索静脉曲张对大鼠生精细胞凋亡的影响。　方法 :采用成年雄性Wistar大鼠复制左精索静脉曲张模型 ;用原位末端脱氧核苷酸转移酶标记法 (TUNEL)检测睾丸生精细胞凋亡。　结果 :实验组的细胞凋亡率显著高于假手术对照组 (P <0 .0 5 )。　结论 :外科所致的精索静脉曲张大鼠睾丸生长减缓 ,生精细胞凋亡增加 ,这可能是其影响生育能力的机制之一。     

实验性精索静脉曲张对大鼠同侧睾丸的影响

目的:利用大鼠动物模型来研究实验性精索静脉曲张(EV)对其同侧睾丸的影响。方法:EV模型的制作是通过在雄性SD大鼠左侧精索内静脉和肾上腺静脉内侧部分结扎左肾静脉。60只雄性青春期SD大鼠随机分为EV持续6、12、18周组(EV6、EV12、EV18组,每组12只)和相应的接受假手术的对照组(每组8只)。于6、12、18周,摘取成功复制成EV模型大鼠(分别为10,8,9只)以及各自接受假手术的对照组大鼠左侧睾丸,检测其Johnsen's评分、生精小管超微结构、睾丸组织睾酮浓度(ITC)和生精细胞的凋亡指数(AI)。结果:EV6、EV12、EV18组Johnsen's评分(6.92±0.52,4.83±0.41,2.95±0.26)和ITC(6.32±0.85,5.17±0.76,4.11±0.69)均显著低于各自对照组[(9.56±0.35,9.63±0.31,9.39±0.46)和(9.64±1.23,9.38±0.69,9.73±0.49)](P<0.05),并且随着精索静脉曲张持续时间的增加而逐渐降低;EV6、EV12、EV18组AI(5.32±1.23,15.21±0.97,21.13±1.12)显著高于各自对照组(3.21±1.15,3.43±1.21,3.61±1.15),并且随着精索静脉曲张持续时间的增加而逐渐升高;各EV组生精小管的超微结构损伤也随着精索静脉曲张持续时间的增加而逐渐严重。结论:EV导致同侧睾丸ITC下降、生精功能的进行性损害、生精细胞AI增加。     

大鼠精索静脉曲张睾丸组织的蛋白质组学研究

目的精索静脉曲张是男性不育的首要病因,本课题利用蛋白组学技术筛选精索静脉曲张大鼠睾丸中表达变化显著蛋白,探索精索静脉曲张引起不育的可能分子机制。方法雄性Wistar大鼠16只,随机分为对照组和精索静脉曲张实验组。实验组大鼠施行左肾静脉部分结扎术来诱导产生精索静脉曲张,对照组行假手术作为阴性对照。30d后,收集全部大鼠的左侧睾丸。采用原位末端脱氧核苷酸转移酶标记（Tunel）法检测两组大鼠生精细胞凋亡情况,进而比较两组生精细胞的凋亡指数。利用双向凝胶电泳技术（2-DE）寻找两组大鼠睾丸中表达有显著性差异的蛋白质点,然后进行基质辅助激光解析离子化飞行时间质谱鉴定（MALDI-TOFMS）。结果双向凝胶电泳结果显示21个蛋白质点在两组间存在显著性差异。在精索静脉曲张大鼠睾丸中有8个蛋白质点表达上升、13个下降,这些蛋白功能涉及蛋白质合成、加工、降解,基因表达调控,信号转导,能量代谢,凋亡,自由基代谢等。Tunel证实实验组大鼠睾丸中生精细胞凋亡指数显著高于对照组,且本实验筛选出14—3—3e、hnRNPF以及LZTFL1三种蛋白与凋亡相关。结论蛋白组学筛选出精索静脉曲张大鼠睾丸差异表达蛋白,并进一步证实精索静脉曲张促进睾丸生精细胞凋亡,其中相关凋亡蛋白对揭示精索静脉曲张引起男性不育的分子机制提供了可能。     

N-硝基-L-精氨酸甲酯对大鼠精索静脉曲张生精细胞Caspase-3酶及bcl-2基因的影响

目的 探讨精索静脉曲张大鼠睾丸组织中一氧化氮(NO)与生精细胞凋亡之间的关系并研究其机制.方法 实验分3组:对照组(假手术组)、曲张组(精索静脉曲张组)和药物组,每组10只大鼠.(45±5)d的SD雄性大鼠复制左侧曲张模型.药物组为术后8周起腹腔注射N-硝基-L-精氨酸甲酯(L-NAME),剂量:50mg/lg·d-1),每组10只大鼠.术后10周用流式细胞仪检测睾丸生精细胞凋亡,分光光度法检测Caspase3活性,用硝酸还原酶法测定睾丸组织NO含量和NOS活性.逆转录.聚合酶链反应(RT-PCR)检测bcl-2基因mRNA的表达.结果 与对照组对应侧相比较,曲张组大鼠双侧睾丸组织NO含量增加,NOS活性显著升高(P＜0.01),流式细胞仪枪测生精细胞凋亡率显著增加(P＜0.01),生精细胞Caspase-3酶的活性显著升高(P＜0.01),bcl.2基因mRNA表达明显减少(P＜0.01).药物组较曲张组大鼠对应侧睾丸组织NO含量、生精细胞凋亡显著减少(P＜0.01),生精细胞Caspase-3酶的活性显著降低(P＜0.01),bcl-2基因mRNA表达明显增加(P＜0.01).结论 精索静脉曲张明显诱导大鼠睾丸组织生精细胞凋亡.其机制可能与NO含量增加,NOS活性升高,生精细胞中Caspase-3酶的活性升高及bcl-2基因表达下调有关.L-NAME对曲张大鼠乍殖细胞有保护作用.曲张对睾丸的影响是是双侧性的.     

实验性精索静脉曲张对大鼠睾丸生精细胞凋亡的影响

目的 :探讨大鼠精索静脉曲张对睾丸生精细胞凋亡的影响 ,阐明精索静脉曲张引起不育的病理机制。　方法 :选用雄性SD大鼠 32只 ,随机分为假手术组、4 5d模型组、6 0d模型组及 90d模型组。采用肾静脉缩窄法建立精索静脉曲张动物模型 ,流式细胞术分别检测各组大鼠睾丸凋亡细胞数及各级生精细胞数。　结果 :假手术组未出现明显的凋亡峰 ,各模型组均出现明显的凋亡峰 ,且随着造模时间的延长凋亡峰增高。　结论 :精索静脉曲张可引起睾丸生精细胞大量凋亡 ,各级生精细胞数减少 ,这可能是精索静脉曲张引起睾丸生精功能障碍从而导致不育的根本机制     

高压氧对精索静脉曲张大鼠生精细胞凋亡及其基因缺氧诱导因子-α、bcl-2及bax表达的影响

目的 观察高压氧(HBO)对精索静脉曲张(VC)SD大鼠生精细胞凋亡的影响,探讨精索静脉曲张导致男性不育的机制.方法 50只青春期雄性SD大鼠,随机抽出10只作为假手术对照组(A).A组只游离左肾静脉不予结扎,其余40只部分结扎左肾静脉建立VC模型,8周后,将建立了VC模型的40只随机分为VC模型组(B)和HBO干预的VC模型组(C),C组用HBO对VC模型进行干预.实验结束后各组大鼠切取双侧睾丸,采用原位末端标记法(TUNEL)检测大鼠睾丸生精细胞凋亡,免疫组织化学检测低氧诱导因子(HIF)-α、bcl-2及bax的基因表达.结果 B组较A组生精细胞凋亡数明显增多,HIF-α、bax表达增强(P＜0.01),bcl-2表达减弱(P＜0.01),bcl-2/bax比值降低;C组大鼠较B组生精细胞凋亡数减少(P＜0.01),HIF-α表达减弱(P＜0.01)、bcl-2/bax比值上升,而与A组比较差异无统计学意义.结论 VC大鼠生精细胞凋亡率增加,HIF-α表达增强、bcl-2和bax基因表达失调为其凋亡机制之一;高压氧可能通过HIF-α、bcl-2和bax基因调控途径调节生精细胞凋亡,从而改善VC所致的生精功能障碍.     

精索静脉曲张大鼠生精小管生精上皮的超微结构研究

目的 通过精索静脉曲张大鼠睾丸的透射电镜观察,了解生精上皮更多的细胞病理现象.方法 雄性Sprayue-Dawley大鼠30只,随机分为(1)精索静脉曲张组(VG)20只,(2)假手术对照组(SOG)10只,按Saypol方法建立左精索静脉曲张模型,处死观察大鼠,取其左、右侧睾丸组织,作透射电镜观察.结果 VG组双侧睾丸均出现生精小管生精上皮的结构损害,左侧相对较重.与SoG组比较,VG的生精上皮变化表现为:支持细胞胞质内出现大量空泡,大量溶酶体出现(72%vs 28%);紧密连接破坏(69%vs31%),线粒体数量减少(160 vs 362), 两者均有显著性差异(P＜0.01);精子顶体形成异常(62%vs38%),尾部线粒体鞘部分缺失,嵴肿胀(71%vs 29%),尾部横切面线粒体数目减少(186 vs 401), 两者均有显著性差异(P＜0.01).结论 生精小管生精上皮的结构损害是由精索静脉曲张导致男性不育的重要原因.     

Involved intrinsic apoptotic pathway of testicular tissues in varicocele-induced rats

Introduction  Increased testicular germ cell apoptosis has been reported in varicocele-induced rats. We studied intrinsic or extrinsic pathway of apoptosis by detecting Bcl-2, caspase-9, caspase-8, and activated caspase-3 expressions in the bilateral testes of experimental varicocele-induced rats. Materials and methods  Experimental left varicocele (ELV) was created by partial ligation of left renal vein in a study group of 24 adult male Sprague–Dawley rats. The other 24 rats were as control group. Eight rats from each group were killed at 4, 8, and 12 weeks following varicocele creation. Testicular tissues of both groups were sampled for TUNEL assay and immunoblotting. Results  Increased apoptotic germ cell was found in the ipsilateral testis of varicocele group at 8 and 12 weeks after operation (P < 0.05). Increased activated caspase-3 expression in the contralateral (right) testis was noted at 12 weeks following varicocele creation (P < 0.05). Conclusions  Our study demonstrates down-regulation of Bcl-2 expression and increased expressions of caspase-9 and activated caspase-3 in the ipsilateral testis of ELV rats at 8 and 12 weeks, indicating gradually increased testicular tissues apoptosis through the intrinsic pathway in varicocele-induced rats. Simultaneously, increased apoptosis in the contralateral testis was observed at 12 weeks (P < 0.05) following varicocele creation also.     

单侧隐睾大鼠生殖股神经在对侧睾丸损害中的作用机制研究

目的 :探讨单侧隐睾大鼠模型生殖股神经在对侧睾丸损害中的作用机制。　方法 :建立单侧隐睾大鼠模型 (2 1d龄 ) ,切断该侧生殖股神经 ,12 0d后观察对侧睾丸的生精细胞凋亡变化及组织乳酸含量变化。　结果 :切断生殖股神经后 ,对侧睾丸生精细胞凋亡为 (5 .76± 0 .76 ) % ,与对照组 (17.2 8± 1.36 ) %相比明显减少 (P <0 .0 5 ) ;乳酸含量也由 (2 .19± 0 .2 4 )mmol/L下降为 (1.70± 0 .31)mmol/L(P <0 .0 5 ) ,且乳酸含量与细胞凋亡呈正相关(P <0 .0 5 )。　结论 :单侧隐睾症对侧睾丸损伤可能与其神经传导反射性血流减少引起生精细胞凋亡有关     

大鼠血清表皮生长因子和睾丸表皮生长因子受体与精子生成的相关性

目的　探讨血清表皮生长因子 ( EGF)和睾丸组织表皮生长因子受体 ( EGF-R)与大鼠精子生成的关系。　方法　 40只性成熟期雄性 SD大鼠 ,随机分为假手术组( SOG)、去颌下腺组 ( SG)、去颌下腺加腹腔注射 EGF I组 ( SG-EGF I)和 II组 ( SG-EGFII) ,每组 1 0只。SG-EGF I和 SG-EGF II分别腹腔内注射 EGF0 .2 5和 0 .50 μg·kg- 1·d- 1。大鼠常规喂养 48d,断头取血和睾丸。放射免疫法检测血清 EGF水平 ,病理检查睾丸生精功能和免疫组织化学检测睾丸组织 EGF-R的表达。　结果　大鼠血清 EGF水平SG-EGF I组明显下降 ( P<0 .0 5) ,SG组有非常显著下降 ( P<0 .0 1 ) ;睾丸生精功能中、重度障碍 ;间质细胞 EGF-R表达明显减少 ( P<0 .0 5)。补充不同剂量的 EGF对睾丸生精功能有不同影响。 SOG、SG-EGF I和 SG-EGF II大鼠睾丸生精细胞、支持细胞及间质细胞 EGF-R表达无显著性差异 ( P>0 .0 5)。　结论　EGF对精子发生具重要的调控作用 ,血清 EGF水平和睾丸间质细胞 EGF-R高表达与睾丸生精功能呈正相关     

The correlation between serum epidermal growth factor/testicular epidermal growth factor receptor and spermatogenesis in rat

<正>Objective: To investigate the correlation between epidermal growth factor (EGF)/testicular epidermal growth factor receptor (EGF-R) and spermatogenesis in rat. Methods: Forty mature male Spraque-Dauley (SD) rats were randomly assigned to four groups, ten rats in each: sham operation group (SOG), sialoadenectomy group(SG), sialoade-nectomy group with injection of EGF (0. 25 μg·kg-1·d-1, SG-EGF Ⅰ) and sialoadenectomy group with injection of EGF (0. 50 μg·kg-1·d-1 , SG-EGF Ⅱ). The rats were routinely feed, and blood and testes were obtained on the 48th day after the operation. Serum EGF concentrations were determined by radioimmunoassay (RIA) , expression of EGF-R in testes was examined by the immunohistochemical method, and the spermatogenesis was pathologically checked. Results: Serum EGF levels in SG-EGFIand SG decreased significantly when compared with those of SOG (P<0. 05 and P< 0. 01, respectively). The testicular function of spermatogenesis showed a moderate to severe impairment in SG. The expression of EGF-R in Leydig cells decreased in SG(P<0. 05). The two dosage groups of EGF replacement had different effects. There were no significant differences of EGF-R expression in testicular germ cells, Sertoli cells and Leydig cells in SOG, SG-EGFⅠand SG-EGFⅡ(P>0. 05). Conclusion: EGF may play an important role in the regulation of spermatogenesis. Serum EGF concentration and high expression of EGF-R in Leydig cells have a positive correlation with spermatogenic function of the testes.     

Apoptosis-related proteins in the testes of infertile men with varicocele

OBJECTIVES: To assess immunohistochemically the expression of apoptosis-related proteins in the testes of infertile men, to determine which of these proteins were related to hypospermatogenesis, as a previous report suggested that apoptosis was suppressed in infertile men with varicocele. MATERIALS AND METHODS: The expression of Bcl-2, Bax, caspase-1 (ICE) and caspase-3 (CPP32) were examined in bilateral testicular specimens from 26 infertile men with varicocele and six normal testicular specimens, using the avidin-biotin-peroxidase complex method. Clinical variables were also assessed. RESULTS: Bax, ICE, and CPP32 were expressed in germ cells, while Bcl-2 was not. Differences in staining in left or right testes were not significant. In both testes of infertile patients with varicocele, significantly fewer germ cells stained for CPP32 than in controls (P < 0.001). For Bax and ICE, total germ cell staining was similar between these groups. Staining was less frequent in infertile patients for both CPP32 and ICE when the analysis was restricted to spermatogonia. Serum luteinizing hormone levels correlated positively with CPP32 staining (P = 0.0457). CONCLUSIONS: The reduced expression of CPP32 participates in regulating apoptosis in the testes of infertile men with varicocele.     

Effects of experimental varicocele require neither adrenal contribution nor venous reflux

Experimental left varicocele (ELV) is known to induce bilateral changes in the rat testis that, where comparisons are possible, are similar to the changes induced by unilateral varicocele in the human. In the present study, we have determined whether or not left adrenal products are important to the changes induced by ELV and whether or not reflux of left renal vein content occurs in the ELV rat. In the first study, testicular blood flow and temperature were studied in control animals and those with ELV, left adrenalectomy (LAX), or ELV + LAX. Control left and right testicular blood flow (33.6 +/- 0.8 and 33.6 +/- 1.5 ml./min./100 gm. tissue respectively) was significantly elevated by ELV (to 39.9 +/- 0.9 and 41.2 +/- 2.7 ml./min./100 gm. tissue, respectively) and the difference between abdominal and testicular temperatures (delta T) was significantly reduced. Control delta T's for right and left testes were 3.2 +/- 0.2C and 3.2 +/- 0.2C, respectively, and right and left delta T's for ELV animals were 2.0 +/- 0.3 degrees C and 2.0 +/- 0.3C, respectively. These blood flow and temperature changes also occurred when ELV animals were subjected to simultaneous LAX. Additionally, when 85Sr-labelled microspheres were infused into the left renal vein, they did not appear in either left or right testes of ELV animals. We conclude that there is no evidence for reflux down the spermatic vein in ELV in rats and adrenal products do not reach the testis via this route after being secreted into the renal vein. We raise the suggestion that the same may be true in the human.     

Effect of cocaine on germ cell apoptosis in rats at different ages

Aim:To investigate the effect of cocaine on apoptosis and caspase-3 activity in germ cells in male rats at differentages.Methods:Cocaine hydrochloride was given(15 mg/kg body weight s.c.)to male Sprague-Dawley rats of3 weeks(n=8),6 weeks(n=8)and 12 weeks(n=8)of age,daily for 28 days.The serum levels of folliclestimulating hormone(FSH),luteinizing hormone(LH),prolactin(PRL),testosterone(T)and estrogen(E_2)wereassayed,and the DNA fragmentation of germ cells was determined by gel eletronphoresis.The cell cycle,apoptosisand caspase-3 activity of germ cells were tested by flow cytometry.Results:After the 28-day cocaine treatment,testes weight of the 3-week-old rats,the testes and body weights of the 6-week-old rats were decreased significantlycompared to those of their corresponding controls(P<0.05).The serum level of T was decreased significantly inthe 3-week-old and 6-week-old rats,and the serum level of PRL was also decreased significantly in 12-week-old ratscompared to the controls(P<0.05).In all the three cocaine-treated groups,the isolated DNA displayed a clear ladderpattern,especially in the 6-week old rats.The number of apoptosic germ cells increased significantly in 3-and 6-week-old rats treated with cocaine(P<0.05).The caspase-3 activity in all three groups increased significantlycompared to the controls(P<0.05),especially in the 6-week-old rats.Conelusion:Cocaine exposure for 28 daysleads to significant damage to male gonad and apoptosis elevation in testes of rats of different ages,especially in thoseof 6 weeks of age.The increase in caspase-3 activity might be a key pathway related to the early stage of apoptosisas the mechanism of cocaine-induced germ cell loss.(Asian J Androl 2006 Sep;8:569-575)     

大鼠一侧睾丸扭转对侧睾丸改变的实验研究

目的 :研究一侧睾丸扭转 (UTT)后对侧睾丸组织学及生精细胞凋亡的改变 ,以明确UTT后对侧睾丸是否存在损伤。　方法 :SD雄性大鼠 6 0只 ,随机分为实验组 (n =4 8)及对照组 (n =12 )。实验组采用Turner方法建立左侧睾丸扭转模型 ,于扭转后 6h处死 4只 ,其余 4 4只再分为扭转睾丸复位及切除组 ,分别于术后 1d、1周、4周处死7～ 8只 ,取睾丸组织进行组织学及生精细胞凋亡的检测。　结果 :UTT复位后对侧睾丸组织学发生明显改变 ,生精细胞凋亡指数明显高于对照组 (P <0 .0 5 )。扭转睾丸切除后对侧睾丸变化不明显。　结论 :UTT可引起对侧睾丸损伤 ,其机制可能与再灌注有关 ,扭转睾丸切除可防止或减轻对侧睾丸的损伤     

大鼠睾丸扭转后生殖细胞凋亡与Bcl-2和Bax基因表达

目的 :研究睾丸扭转复位后生殖细胞凋亡与Bcl 2 /Bax基因表达的关系。　方法 :30只成年健康SD雄性大鼠随机分成扭转组 (n =15 )和对照组 (n =15 )。建立单侧睾丸扭转复位动物模型 (72 0° 2h)。术后 3d取手术侧睾丸 ,采用原位缺口末端标记法 (TUNEL)检测生殖细胞凋亡 ,免疫组化SP法检测Bcl 2 /Bax基因表达。　结果 :与对照组相比 ,扭转组手术侧睾丸生殖细胞凋亡显著增多 (P <0 .0 1) ,Bax表达显著升高 (P <0 .0 1) ,Bcl 2表达显著降低 (P <0 .0 1)。凋亡细胞主要是初级精母细胞和圆形精子细胞。　结论 :睾丸扭转复位后 ,生殖细胞凋亡与Bcl 2 /Bax基因表达密切相关。细胞内Bcl 2 /Bax比值是影响生殖细胞凋亡的重要因素之一。