下咽鳞癌患者血清比较蛋白质组学研究

Objective To detect the serum proteomic patterns in hypopharyngeal squamous cell carcinoma (HPSCC) patients. Methods The study included 58 HPSCC patients and 41 normal controls. The serum samples were analyzed for protein expression using by SELDI-TOF-MS protein chip array technology and cation-exchange (CM10) Protein array. Results There were 9 peaks differentially expressed between HPSCC patients and normal controls (P<0. 05). Using tree analysis pattern, the combination of these biomarkers could discriminate HPSCC from normal controls with a total accuracy of 90. 10%. There was no statistically significant difference in serum proteins between HPSCC patients with and without cervical lympha node metastasis ( P > 0.05 ) , and the difference between well-differentiated and poorly-differentiated cancer was quite minor. There was no any statistically significant difference between chemotherapy sensitive patients and nonsensitive patients (P>0. 05) , but 5 kinds of differentially expressed protein between radiotherapy sensitive patients and nonsensitive patients (P≤0. 05). The radiotherapy sensitive patients were segregated from nonsensitive patients with a sensitivity of 100. 00% and specificity of 83. 33%. Conclusion There are 4 peaks differentially expressed between HPSCC patients and normal controls ( P < 0.01). The serum protein at the m/z value of 6115. 74 of HPSCC radiotherapy sensitive patients is expressed higher than radiotherapy nonsensitive patients (P<0.01).     

下咽鳞癌患者血清比较蛋白质组学研究

Objective To detect the serum proteomic patterns in hypopharyngeal squamous cell carcinoma (HPSCC) patients. Methods The study included 58 HPSCC patients and 41 normal controls. The serum samples were analyzed for protein expression using by SELDI-TOF-MS protein chip array technology and cation-exchange (CM10) Protein array. Results There were 9 peaks differentially expressed between HPSCC patients and normal controls (P<0. 05). Using tree analysis pattern, the combination of these biomarkers could discriminate HPSCC from normal controls with a total accuracy of 90. 10%. There was no statistically significant difference in serum proteins between HPSCC patients with and without cervical lympha node metastasis ( P > 0.05 ) , and the difference between well-differentiated and poorly-differentiated cancer was quite minor. There was no any statistically significant difference between chemotherapy sensitive patients and nonsensitive patients (P>0. 05) , but 5 kinds of differentially expressed protein between radiotherapy sensitive patients and nonsensitive patients (P≤0. 05). The radiotherapy sensitive patients were segregated from nonsensitive patients with a sensitivity of 100. 00% and specificity of 83. 33%. Conclusion There are 4 peaks differentially expressed between HPSCC patients and normal controls ( P < 0.01). The serum protein at the m/z value of 6115. 74 of HPSCC radiotherapy sensitive patients is expressed higher than radiotherapy nonsensitive patients (P<0.01).     

原发性前列腺鳞癌1例报告

原发性前列腺鳞癌罕见,约占前列腺恶性肿瘤的0.2%～1%[1],早期诊断困难,恶性程度高,预后差.国内外正式报道的病例都很少,临床治疗方案尚不统一.现报道我院诊治的1例前列腺鳞癌患者,并回顾国外有关此病的治疗作一报道.     

原发性前列腺鳞癌1例报告并文献复习

目的:提高对原发性前列腺鳞癌的病因及诊治的认识。方法:回顾性分析1例64岁男性原发性前列腺鳞癌患者临床资料:经病理检查诊断为原发性前列腺鳞癌,行手术治疗后辅以放疗,并复习相关文献,探讨本病的病因、诊治及预后。结果:患者术后随访11个月,仍存活。结论:原发性前列腺鳞癌临床罕见,恶性程度高,肿瘤进展快,预后差;对内分泌治疗无效,早期手术治疗并辅以放化疗效果明显。     

肝内胆管癌细胞系ICC-9810与肝细胞癌细胞系SMMC-7721蛋白质组学比较

目的 比较肝内胆管癌细胞系ICC-9810、肝细胞癌细胞系SMMC-7721及正常肝细胞系L02的蛋白质表达,筛选胆管细胞癌和肝细胞癌的标志蛋白.方法 收集体外培养的肝内胆管癌细胞系ICC-9810、肝细胞癌细胞系SMMC-7721及正常肝细胞系L02细胞,细胞裂解液处理后提取蛋白.应用表面增强激光解吸离子化(SELDI)蛋白质芯片技术同时使用IMAC3和WCX2两种芯片检测肝内胆管癌细胞系ICC-9810、肝细胞癌细胞系SMMC-7721及正常肝细胞系L02的蛋白质谱.用PBSII-C型蛋白质芯片阅读机读取数据,采用Proteinchip Software 3.0.2软件分析数据.结果 与L02细胞比较,有12个蛋白质在两种肝癌细胞系中均出现明显的变化,其中4个蛋白高表达,8个蛋白低表达.比较两种肝癌细胞系的蛋白质谱,发现7个蛋白在ICC-9810细胞中高表达,10个蛋白低表达;11个蛋白在SMMC-7721细胞中高表达.在L02和ICC-9810细胞中去磷酸化蛋白17 175 Da表达较多,而在SMMC-7721细胞中磷酸化蛋白17 255 Da表达较多.结论 肝内胆管癌、肝细胞癌与正常肝细胞的差异蛋白表达为寻找肝肿瘤标志物提供了重要线索.17 255 Da蛋白的磷酸化/去磷酸化可能与肝细胞癌的发生有关.     

肝总管鳞癌1例

患者　男 ,5 0岁。入院前 2 0d无明显诱因出现进行性巩膜、皮肤黄染 ,尿色加深。无腹痛 ,无发热、寒战 ,无服药史。 10年前起患乙型肝炎、肝炎后肝硬化。实验室检查 :血清白蛋白2 3 6g/L ,AST 2 3 0U /L ,ALT 2 3 5U /L ,r GT 465U /L ,总胆红素 186.2 μmol/L ,结合胆红素 12 9.0 μmol/L ,总胆汁酸165 μmol/L。B超 :结节性肝硬化、脾大 ,肝总管扩张 ,内见 2 .5cm× 1.8cm增强光团 ,肝内胆管扩张。CT示肝硬化 ,脾大 ,肝总管扩张伴实质性占位 ( 2 .6cm× 2 .0cm ) ,肝内胆管扩张。择期手术。手术中发现肝脏明显萎缩、淤胆、满布大…     

蛋白质组学技术及其在膀胱癌诊断中的研究

蛋白质组学是生命科学研究的一门新兴学科,已广泛应用于肿瘤研究,现将其在膀胱癌诊断研究中的进展作一综述.     

食管鳞癌DNA含量与临床病理及预后的关系

应用自动化图像分析仪对５０例食管鳞癌进行细胞ＤＮＡ定量分析，结合临床资料，发现肿瘤分化越差、外侵越严重，ＤＮＡ含量越高。淋巴结转移组ＤＮＡ含量高于非转移组。二倍体或近二倍体组１、３、５年生存率明显高于异倍体组（Ｐ＜０．０１）。表明ＤＮＡ含量的测定可从核酸代谢的分子水平揭示食管癌恶性生物学行为，同时可作为估计手术预后的客观定量指标。     

胆囊鳞癌和腺鳞癌12例临床分析

目的总结原发性胆囊鳞癌和腺鳞癌的诊治经验。方法回顾性分析了解放军总医院肝胆外科1994—2004年收治的胆囊癌12例（鳞癌4例，腺鳞癌8例），男女各6例。结果行根治性手术9例，姑息性手术3例。12例中有11例得到随访，其中2例在1年后死亡，1例在半年后死亡，其余均在半年内死亡。术后中位生存期108d。结论胆囊鳞癌和腺鳞癌恶性程度高，但远处转移较少，有利于手术切除。术后放疗有可能是一个有效的方法。     

Pharyngeal angiosarcoma following multimodal treatment for oropharyngeal squamous cell carcinoma

It is well established that angiosarcoma can develop following radiotherapy. We present an unusual case of angiosarcoma of the pharynx that developed three years after treatment with surgery and adjuvant chemoradiotherapy for a T2N2bM0 squamous cell carcinoma of the oropharynx. The patient was tumour free until developing dysphagia, which was found to be caused by an angiosarcoma. The patient underwent surgery of the pharyngeal angiosarcoma by laryngopharyngectomy, tongue base resection, selective neck dissection and radial forearm microvascular free flap reconstruction. Angiosarcoma following head and neck malignancy is rare but must be considered as part of the differential diagnosis in patients with new symptoms after radiotherapy.     

胆囊鳞癌和腺鳞癌12例临床分析

目的总结原发性胆囊鳞癌和腺鳞癌的诊治经验。方法回顾性分析了解放军总医院肝胆外科1994-2004年收治的胆囊癌12例(鳞癌4例,腺鳞癌8例),男女各6例。结果行根治性手术9例,姑息性手术3例。12例中有11例得到随访,其中2例在1年后死亡,1例在半年后死亡,其余均在半年内死亡。术后中位生存期108d。结论胆囊鳞癌和腺鳞癌恶性程度高,但远处转移较少,有利于手术切除。术后放疗有可能是一个有效的方法。     

Subungual squamous cell carcinoma

Squamous cell carcinoma of the nail bed is quite uncommon and is often mistaken for other conditions, causing a delay in diagnosis which, once the condition metastasises, can be fatal. We present the case of an 89-year-old man with a 2-year history of a painful lesion under his nail bed in whom histology confirmed subungual squamous cell carcinoma, and who underwent amputation at the level of the interphalangeal joint of his right thumb. It is important that the presence of a rapidly growing tumour suggest the possibility of malignancy, as early and exploratory nail plate removal for the evaluation of any suspicious subungual lesions may allow the avoidance of amputation.     

Micro-invasive squamous cell carcinoma arising in a pre-existing intraventricular epidermoid cyst

Summary The authors report the case of a 67-year-old woman with primary micro-invasive squamous cell carcinoma located in the lateral ventricle and originating from an epidermoid cyst. Radiological and histological features of the neoplasm are described and the relevant literature is studied briefly.Two consecutive surgical specimens were studied by light microscopy. In the histological sections of the subtotally removed material of the first operation, the cyst wall was layered by epithelium — the same as expected in an epidermoid cyst — and showed foci with mild to moderate dysplastic changes. Theses changes did not reach up to the degree of a carcinoma. However, because the cyst could not be resected totally, the possibility of a squamous cell carcinoma was considered in the differential diagnosis. In the present case, the diagnosis was firmly established in a second surgical specimen obtained ten months after the first operation. Now, the tumour proved to be a primary squamous cell carcinoma, which exhibited severe dysplastic changes.Primary squamous cell carcinoma of brain is extremely rare. To our knowledge, only 20 such tumours with pre-existing epidermoid cyst have been reported in the central nervous system. The intriguing observation in this case is a three year recurrence free survival following the subtotal removal of the malignant neoplasm at the second surgery, without postoperative radiotherapy.     

同卵双胞胎食管癌患者的血清蛋白组学研究

目的 观察河南食管癌高发区同卵双胞胎食管癌患者与高发区散发食管癌患者血清蛋白的表达,筛选食管鳞癌相关血清标记物.方法 收集3对同卵双胞胎、5例散发食管癌患者的血清,采用磁珠蛋白组技术对3对同卵双胞胎中的食管癌患者和10对健康同卵双胞胎(对照组)的血清进行蛋白组对比.结果 在3对同卵双胞胎中的3例食管癌患者中,共同表达的差异蛋白为16个.3对同卵双胞胎中的3例食管癌患者和20对健康同卵双胞胎对比差异蛋白为5905.29、5967.68、1779.04 Da(P＜0.05),5例散发食管癌与10对健康人对比差异蛋白为1779.17、1929.57、1866.43、1881.41、5095.48 Da(P＜0.05).结论 应用磁珠蛋白技术在同卵双胞胎中筛选出的差异蛋白可能成为食管癌筛查的血清蛋白标志物.     

The use of flowcytometry in analysis of oral and maxillofacial squamous cell carcinoma

One hundred cases of squamous cell carcinoma in oral and maxillofacial regions, including 50 fresh samples and 50 paraffin-embedded blocks, were examined with the use of Flow-cytometry (FCM) for DNA content of tumour cells, changes in ploidy, and kinetics of tumour cells. The results showed that: the average DNA Index (DI) was 1.154; the incidence of heteroploidy was 59%; and the S-phase cell proportion (PI) was 22.62%. Following the advance of tumour, as assessed by clinical stage and pathological grade, the rate of heteroploidy, DI and PI increased markedly. The results demonstrate that FCM can be utilized as an adjunct method for pathologic diagnosis with particular importance in the evaluation of malignant grade of neoplasms. This study programme was sponsored by the China National Foundation for Natural Sciences.     

Mixed large cell neuroendocrine carcinoma with squamous cell carcinoma of the rectum: Report of a rare case and review of the literature

INTRODUCTIONMixed large cell neuroendocrine neoplasms of the rectum are rare and aggressive neoplasms. Survival is poor due to the high rate of lymph node metastases and distant metastases at the time of diagnosis.PRESENTATION OF CASEWe report a case of a 50-year-old male patient with a mixed large cell neuroendocrine carcinoma with squamous cell carcinoma of the rectum located 8 cm from the anal verge, treated with low anterior resection and total mesorectal excision with free surgical margins. There were lymph nodes metastases but no distant metastases at the time of diagnosis. The patient refused to receive adjuvant chemotherapy and died 6 months later due to liver failure as a result of multiple hepatic metastases.DISCUSSIONThere are not known predisposing factors for the development of neuroendocrine rectal carcinoma. A neuroendocrine carcinoma of the rectum is a rare tumor with an incidence of less than 0.1% of all colorectal malignancies. The median survival ranges from 5 to 10.4 months in several studies and there are not sufficient data in bibliography about ideal adjuvant therapy after resection of mixed squamous large cell neuroendocrine carcinoma of the rectum.CONCLUSIONLow anterior resection and total mesorectal excision with free surgical margins in the presence of lymph nodes metastasis is not a sufficient treatment for rectal neuroendocrine carcinoma. More studies should be done in order to determine the ideal adjuvant treatment of these rare and aggressive tumors.