甲氨喋呤与叶酸的燐光测定

研究了甲氨喋呤的燐光性质,其激发光波长为346 nm,发射光波长为454 nm,在酸中燐光最强,可检测量为5 ng。在碱中无燐光,氧化后燐光也消失。燐光寿命为0.41 s。叶酸在酸中与碱中燐光强度相同,在酸中激发光波长在330 nm,发射光波长为450 nm,在碱中相应蓝移至282与422 nm,最小检测量为10 ng,燐光寿命为0.22 s。血液样品可用三氯醋酸沉淀蛋白后上清液直接测定甲氨喋呤与叶酸,回收率达96～99%,操作简便,符合临床监测分析要求。     

吲满氨酯的磷光分析

吲满氨酯具有强的磷光,其水液激发波峰304 nm,发射波峰434 nm,磷光寿命为3.87s。在碱液中磷光比在酸液中强。重原子碘对吲满氨酯磷光有增强作用,而寿命变短。金属重原子Cu²⁺,Fe³⁺,Pb²⁺,Tl³⁺,Zn²⁺及钼酸铵等均无增强作用,Cu²⁺,Fe³⁺等反有猝灭作用。比较了9种吲满氨酯类似物的磷光性质,当2位亚甲基氧化为酮基后磷光明显下降。建立了吲满氨酯的磷光分析法,最小检出量为5ng。小鼠腹腔注入药物0.1 mg/kg后10 min其血药浓度为149 ng/ml。     

薄层荧光扫描法测定大力王糖浆中7—甲氧基香豆素

用乙醚振摇提取、乙醇定容后,在硅胶G CMC薄层板上用甲苯-醋酸乙酯-甲酸(5:1:0.1)为展开剂,分离大力王糖浆中的7-甲氧基香豆素,用岛津CS-910薄层扫描仪在激发波长325nm,发射波长400nm 处,荧光反射法线性扫描对7-甲氧基香豆素进行了含量测定.回收率平均值为103.11%,Cv 为2.77%,本方法简便、快速,重现性好。     

血清中异丙酚的HPLC测定

建立了检测血清中异丙酚的反相高效液相色谱-荧光法.血清样品经含内标百里酚的丙酮溶液直接沉淀后,采用KR100-5 C18柱,甲醇-0.1%三氟醋酸溶液(80:20)为流动相,荧光激发波长为276nm,发射波长为307nm.异丙酚在0.05～12.8μg/ml范围内与峰面积比线性关系良好.检测限为150pg,异丙酚和百里酚的提取回收率均大于90%,批内、批间精密度为1.11%～6.88%.     

牛奶中左氧氟沙星的铽络合物荧光增敏法测定

建立了铽（Tb^3＋）-左氧氟沙星络合物荧光增敏法测定牛奶中左氧氟沙星的含量。左氧氟沙星与Tb^3＋结合后，543nm处出现络合物的强荧光峰。最终确定了测定条件：200倍的Tb^3＋，pH6．0醋酸-醋酸铵缓冲溶液，激发波长和发射波长为291和543nm。左氧氟沙星在0．01～2μg/ml范围内，浓度与荧光强度成良好的线性关系，检测限为2．5ng／ml。     

抗凝药物华法林的荧光光谱测定方法研究

目的:基于华法林钠的荧光光谱特性,以水-乙醇混合溶剂为介质,研究建立了一种测定华法林钠的荧光光谱分析方法。方法:水-乙醇的最适宜配比为3:7,该体系的最大激发波长(λ_(ex))和发射波长(λ_(em))分别为308 nm 和386 nm,测定系列标准溶液在测定波长下的荧光强度,并计算样品中华法林钠的含量。结果:在所选的最佳试验条件下,华法林钠浓度在0.3～7.1μg·mL~(-1)范围内与荧光强度具有良好的线性关系,检出限为0.1μg·mL~(-1),回收率为96.7%～104.9%。结论:本方法可应用于药物华法林钠含量的测定。     

HPLC测定佐米曲普坦片的含量

目的 采用HPLC测定佐米曲普坦药片的含量.方法 采用Kromasil ODS-3 C18柱(250 mm×4.6 mm,5 μm);0.05%三乙胺溶液(磷酸调pH2.75)-乙腈(90∶10)为流动相;荧光检测器检测(激发波长为255 nm,发射波长为360 nm).结果 佐米曲普坦浓度1～ 80 μg · ml-1与峰面积的线性关系良好(r=0.9998).结论 所建立方法准确,回收率高.     

高效液相色谱法测定人体血浆中氯丙嗪的血药浓度

本文报告了用高效液相色谱法测定人体血浆中氯丙嗪的血药浓度的测定方法,血样采用以乙腈除蛋白的预处理方法,以异丙嗪为内标物质,色谱条件为:色谱柱(15cm×4mm)Noclcsoil C_(18)(15μm),流动相为0.5%NH_4H_2PO_4和甲醇(15∶85),其中内含0.2%乙二胺,紫外检测波长254nm,氯丙嗪与异丙嗪的保留时间为4min50s和7min10s,通过计算峰高比求出氯丙嗪的含量,氯丙嗪在50～300ng/ml范围内呈线性(r=0.9826),血浆中的最低检测浓度0.02μm/ml,回收率94.8%～100.2%,日内变异系数在4.8%以下。     

高效液相色谱法测定屈螺酮炔雌醇片的有关物质

目的建立屈螺酮炔雌醇片有关物质的检查方法。方法采用高效液相色谱法,Prontosil ACEeps色谱柱和Chromolith RP 18色谱柱串联,流动相A为乙腈-甲醇-水(203∶150∶550),流动相B为乙腈-甲醇-水(595∶150∶50),流速0.35 mL·min~(-1),进行梯度洗脱。紫外检测器和荧光检测器串联使用,(1)紫外检测波长222 nm(0~60 min);(2)荧光检测在0~37 min和42~60 min,激发波长215 nm,发射波长315 nm;在37~42 min,激发波长215 nm,发射波长344 nm;进样量为20μL。结果屈螺酮、炔雌醇与各杂质能有效分离,进样量在线性范围与峰面积线性关系良好(r为0.9998~0.9999),屈螺酮和炔雌醇的检测限分别为0.137μg·mL~(-1)和0.035μg·mL~(-1)(紫外检测),Δ9,11-炔雌醇和炔雌醇的检测限分别为0.0034μg·mL~(-1)和0.0042μg·mL~(-1)(荧光检测)。结论建立的方法准确,灵敏度高,可用于检查屈螺酮炔雌醇片的有关物质。     

用同步荧光光谱法测定脂质过氧化物

脂质过氧化物（LPO）是游离的或结合的不饱和脂质酸受自由基作用而形成的过氧化物，可进一步断裂生成丙二醛。人体内脂质过氧化物水平与某些生理病理过程的关系极为密切，其含量的测定也越来越受到人们的重视。在LPO的测定中，用的较多的是Yagi法[1]。在酸性加热条件下，LPO分解产生的丙二醛与硫代巴比妥酸反应生成红色物质，该化合物是荧光性物质，其荧光激发峰波长为532um，发射峰波长553um。由于两峰波长相近，在常规测定时采用激发波长515um，发射波长553um，使荧光产率有所损失，影响了测定灵敏度。而采用同步荧光光谱法测定时，通…     

糖衣对盐酸氯丙嗪片含量测定的影响

目的:研究糖衣对盐酸氯丙嗪片含量测定的影响。方法:采用紫外分光光度法。先取空白糖衣溶液在200～400nm波长范围内进行紫外扫描,再分别对4个厂家的样品经剥糖衣和不剥糖衣处理后在254nm波长处测定吸光度并计算含量,并将数据进行配对设计的t检验。结果:空白糖衣溶液在200～400nm波长范围内无吸收;剥糖衣和不剥糖衣处理后样品含量测定结果无显著性差异(P>0.05)。结论:糖衣不影响盐酸氯丙嗪片的含量测定结果,盐酸氯丙嗪片可不剥糖衣进行含量测定。     

Chlorpromazine metabolism. IX. Pharmacokinetics of chlorpromazine following oral administration in man

A single oral dose (120 mg/m²) of Chlorpromazine hydrochloride was administered to four healthy subjects and the blood levels of Chlorpromazine were determined with time. Appropriate equations describing the two-compartment open model with zero-order absorption and the two-compartment model with first-order absorption, both with a lag time, were fitted to the observed data using weighted nonlinear least-squares regression analysis. Fitting the two-compartment model with zero-order absorption and a lag time to the observed data resulted in a significant reduction of the weighted sum of squared deviations, i.e., better correlation between the observed and calculated data, and a closer random scatter of the observed concentration data around the calculated curve with no apparent systematic deviations from the curve. These results suggest that Chlorpromazine absorption is zero order. Chlorpromazine began to appear in the systemic circulation after a mean lag time of 0.4 hr and continued to be absorbed for approximately 2.9 hr. The mean half-lives of the distribution and elimination phases were 1.63 and 17.7 hr, respectively.This work was supported in part by National Institute of Mental Health Grant No. MH 21408-02, NIH.Presented by L. Whitfield at the Twenty-third National Meeting of the APhA Academy of Pharmaceutical Sciences, November 13–17, 1977.     

胺类药物的燐光分析

建立了胺类药物通用的燐光测定法药物与染料-金莲橙在一定pH下生成离子对复合物,可用氯仿提取,提取出的药物—金莲橙经还原转化成有强燐光的产物,λ_ex302 nm,λ_em424 nm。此法灵敏度高,最小检出量可达5 ng。叔胺、仲胺类药物均可用此法分析。     

The Distribution of Chlorpromazine between Plasma and Erythrocytes

Abstract: Chlorpromazine is found to be taken up by the erythrocytes in a dissociable binding similar to that of the plasma protein binding so that an equilibrium is attained between these binding sites. The plasma/cell ratio is on an average 2 but is subject to large interindividual variations. This ratio is only moderately influenced by the presence of other drugs and anticoagulants in the blood. The consequences of the cell binding of drugs for the analytical result when using plasma or whole blood and the evaluation of blood concentrations of their relation to the therapeutic effect are discussed.     

Potentiation of the effects of chlorpromazine on exploration in the rat by a prior experience of the drug

Chlorpromazine increased the latency with which rats started to explore a new environment, and decreased the time they spent in exploring it and the novel objects in it. These effects were significant with a 4 mg/kg, but not with a 2 mg/kg dose. There was a potentiation of these drug effects in animals with experience of the drug in an environment offering little opportunity for exploration (the home cage), and 2 mg/kg now became an effective dose. Thus the responses made to the first drug situation affected the behaviour in the second.     

盐酸氯丙嗪注射液细菌内毒素检查法的实验研究

目的 研究盐酸氯丙嗪注射液对细菌内毒素检查实验的干扰情况，并建立其细菌内毒素检查的质量标准。方法 参照《中国药典》2005年版二部附录XIE细菌内毒素检查法进行实验．应用两个生产厂家的鲎试剂对两个厂家的盐酸氯丙嗪注射液进行干扰试验研究。结果 盐酸氯丙嚷注射液对细菌内毒素检查有干扰作用，但通过盐酸三羟甲基氨基甲烷（Tris）缓冲液（pH7．2）稀释可消除干扰。结论 该制剂可以用细菌内毒素检查法（凝胶法）代替兔热原检查法控制其产品质量。     

启维与氯丙嗪治疗精神分裂症的对照研究

目的评价启维与氯丙嗪治疗精神分裂症的临床疗效和安全性。方法将47例符合CCMD-3精神分裂症诊断标准的患者随机分为两组,分别给予启维与氯丙嗪治疗,于治疗前和治疗后6周末以BPRS、TESS量表评定其疗效和副反应。结果启维与氯丙嗪总体疗效相似,两组比较无显著差异(P>0.05)。而启维组的不良反应少于氯丙嗪组。结论启维是一种安全有效的新一代抗精神病药物,适合门诊精神分裂症病人使用。     

Social behaviour of albino rats: A methodological and pharmacological approach

In order to test the usefulness of motivational hypothesis in the analysis of drug action on rat social behaviour, the frequency with which rats select the section of the cage in which there are other rats was evaluated in control sessions and after drug administration.In the given experimental conditions, the decoy rats become a sufficient stimulus for the acquisition of a discriminating conditioned reflex.Chlorpromazine (2 mg/kg) was without effect, but pentobarbital (3 mg/kg) abolished the power of the decoy rats to influence the behaviour of other rats.For the interpretation of these results it is not necessary to postulate social motivations but it is sufficient to attribute to the decoy rats the properties of a conditioned stimulus.