脱痂Ⅰ号治疗Ⅲ度烧伤34例临床分析

脱痂Ⅰ号是上海瑞金医院研制的新型脱痂药,主要由苯甲酸、乳香、没药、延胡索、冰片、磺胺嘧啶银等组成,为中西医结合药物.1996年3月～1998年3月,我们应用该药治疗Ⅲ度烧伤34例,效果显著.现报告如下.     

痛经贴质量标准研究

目的:建立痛经贴的质量标准。方法:对延胡索乙素和冰片进行了薄层色谱(TLC)鉴别。用高效液相色谱法(HPLC)测定了痛经贴中延胡索乙素的含量。结果:薄层色谱斑点清晰;延胡索乙素在0.0187~0.093mg.m l-1范围内,对照品浓度与峰面积呈良好线性关系,r=0.9999,平均加样回收率为98.80%,RSD为0.97%。结论:方法简便、准确、重现性好、精密度高。可用于控制痛经贴的质量。     

黄芪、当归及其配伍对正常和血虚模型大鼠全血比粘度和血浆比粘度的影响

反相ＨＰＬＣ法测定３种延胡索生物碱含量祝晨陈，何宏贤，金蓉鸾。中国中药杂志，１９９４，１９（２）：７７～７９采用反相ＨＰＬＣ法分析了灰绿延胡素、长距延胡索和对叶延胡索中８种生物碱（延胡索丙素，巴马亭，去氢紫堇碱，延胡索乙素，延胡索甲素，黄连碱，去氢紫...     

延黄冰合剂外敷治疗化疗性静脉炎的临床效果研究

目的：探讨延黄冰合剂对化疗药物输液引发静脉炎的治疗效果,为临床化疗性静脉炎提供中医药治疗方案。方法将输注化疗药的患者随机分成两组,治疗组选用延胡索、大黄、冰片、侧柏叶、蒲黄等几味中草药调制成的“延黄冰合剂”湿敷治疗。对照组选用50%硫酸镁湿敷治疗。结果治疗组总有效率治疗组87.07%,对照组41.23%,二者有差异（P＜0.01）。两组疼痛缓解时间及静脉炎有效时间比较,二者有差异（P＜0.01）。结论延黄冰合剂治疗静脉炎显效快、使用安全,能有减轻化疗性静脉炎的损害。     

HPLC法测定延胡索止痛滴丸中延胡索乙素

延胡索止痛滴丸是以延胡索为原料经提取加工制备而成的滴丸剂型,具有活血、利气、止痛的功能,用于胸胁、脘腹疼痛、经闭痛经、产后瘀阻、跌打肿痛。方中延胡索的有效成分延胡索乙素具有较强的镇痛作用。本实验采用HPLC法测定延胡索止痛滴丸中延胡索乙素,作为质量控制的指标,结果表明方法操作简单、准确、重现性好。1仪器与材料美国戴安高效液相色谱仪,UVD340U二极管阵列检测器,ASI—100自动进样器,AL系列溶剂过滤器,PeakNet色谱工作站;CP224S型分析天平(北京赛多利斯仪器系统有限公司);延胡索乙素对照品(中国药品生物制品检定所);延…     

不同透皮吸收促进剂对延胡索乙素透皮吸收的影响

【目的】考察不同透皮吸收促进剂对延胡索乙素透皮吸收的影响,为促透剂的选择提供依据。【方法】采用Franz体外释药装置,紫外分光光度法检测接受液中延胡索乙素的浓度,通过计算药物的累积透皮百分率研究延胡索乙素经离体鼠皮的透皮规律。考察了不同种类及浓度促透剂对延胡索乙素体外透皮特性的影响。【结果】延胡索乙素透皮吸收与其浓度有关,促透剂的种类和用量对其皮肤透过量均有影响。250μg/ml浓度的延胡索乙素透皮吸收效果较好,2%冰片的促透作用较其它促透剂作用更强。【结论】选择适当的透皮吸收促进剂可显著提高延胡索乙素的经皮渗透速率,延胡索乙素有望开发成经皮给药制剂。     

元胡止痛冲剂中延胡索乙素含量测定

元胡止痛冲剂,由延胡索、白芷等中药组成,具有理气活血止痛之功效.用于气滞血瘀的胃痛,头痛及月经痛等.延胡索为本品主要成分之一,延胡索乙素为主要有效成分.采用薄层扫描法对元胡止痛冲剂中延胡索乙素的含量进行了测定,得到了满意的结果.     

中药外敷治疗晚期肝癌疼痛14例

介绍应用中药干蟾皮、大腹皮、桃仁、红花、大黄、延胡索、莪术、以、木也香、没药水蛭、冰片外敷治疗晚期肝癌疼痛的经验,收到明显疗效。     

冰片的主要成分及工艺研究进展

目的:通过对冰片的本草沿革的考证,阐述了中药冰片的药用历史、品种发展、各品种的主要成分、提取与纯化工艺进展.历史沿革中的冰片正品应为右旋龙脑.对四种来源的冰片的成分进行了归纳:龙脑冰片、天然冰片.艾片的成分比较简单,一般含有一种.比如艾片主要含左旋龙脑、前两种冰片主要含有的是右旋龙脑.而用α-蒎烯合成的冰片成分比较复杂,含有多种龙脑的成分.用右旋樟脑合成的冰片主要含右旋龙脑及右旋异龙脑.冰片的提取以通汽水蒸汽蒸馏较优,冰片的精制以化学提纯法工艺较优.     

酸性染料比色法测定延胡索总生物碱的量

延胡索为罂粟科植物延胡索Coryclagyanhusuo W.Twang的干燥块茎,亦名元胡,其味辛、苦,性温;归肝、脾经.具有活血、利气、止痛之功效,临床上主要用于各种疼痛、心血管及痛经等.产于浙江省东阳、磐安、缙云等地,为道地药材"浙八味"之一,其主要药效物质是延胡索总生物碱[1,2].本实验采用紫外分光光度法对延胡索中总生物碱的量进行测定.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.