白细胞介素10基因转染对小鼠心脏移植排斥反应中细胞因子表达的影响

目的 :探讨白细胞介素 10 (IL 10 )基因转染对小鼠心脏移植排斥反应中IL 12、IL 15、IL 18和IL 4表达的影响。方法 :采用小鼠颈部心脏移植模型 ,随机分为 3组 :对照组、移植组和IL 10组。于术后第 5天取移植心脏 ,用逆转录聚合酶链式反应 (RT PCR)法观察IL 12、IL 15、IL 18、IL 4及IL 10的表达情况。结果 :移植组IL 12、IL 15、IL 18表达与对照组比较明显升高 ,IL 10、IL 4表达显著降低 (均P <0 .0 1)。IL 10组IL 12、IL 15、IL 18表达与移植组比较明显降低 ,而IL 4及IL 10表达显著升高 (均P <0 .0 1)。结论 :IL 10基因转染抑制心脏移植排斥反应主要与其抑制IL 12、IL 15、IL 18等Th1型细胞因子的表达 ,促进Th2型细胞因子IL 4的表达 ,使免疫反应由Th1型向Th2型偏移有关     

白细胞介素-1β在心脏移植排斥反应中的作用

目的 :研究白细胞介素 (IL) 1β在大鼠心脏移植排斥反应中心脏局部的表达变化情况 ,探讨其在心脏移植急性排斥反应诊断中的可能作用。方法 :建立大鼠颈部异位心脏移植模型 ,不同品系大鼠之间的移植供、受体分别为SD大鼠和Wistar大鼠 (共 5 0只 ) ,同品系大鼠之间的移植供受体均为Wistar大鼠 (共 5 0只 )。观察移植心脏自然生存时间 ,另于术后第 1、3、5、7、9、11天 ,分别切取移植心脏各 5只 ,提取总RNA ,进行逆转录 ,将目的引物和对照引物在同一管中扩增 ,以两者像素值比值均数作曲线图 ,观察排斥反应时IL 1βmRNA的动态表达情况。结果 :不同品系移植心脏的停跳高峰出现在术后第 6～ 10天。排斥反应时移植心脏局部IL 1βmRNA的表达水平明显增高 ,峰值在术后第 1天。结论 :IL 1β在心脏移植排斥反应中可能起重要的作用 ,IL 1βmRNA的动态检测可望成为早期诊断急性排斥反应的指标     

白细胞介素-10在肝纤维化中的作用

本文简要综述了白细胞介素-10的分子特性、生物学效应及在肝纤维化中的作用机制。     

白细胞介素10与动脉粥样硬化

炎症是动脉粥样硬化 (AS)发生发展的重要环节 ,包括炎性细胞浸润 ,大量的炎症因子表达 ,以及各种炎症因子参与调节的细胞增殖、凋亡 ,细胞外基质重排以及血管重塑。除致炎因子外 ,抗炎因子如白细胞介素10 (IL- 10 )也在 AS斑块中呈现表达拮抗炎性因子的作用 ,成为机体代偿性保护机制之一。1　白细胞介素 10六十年代 ,Mosmann等根据细胞因子的优势分泌效应 ,提出存在二类 T辅助细胞 (TH1和 TH2 )的假说 ,即 TH1细胞分泌 IL - 2 ,IFN-γ,主要诱导巨噬细胞活化和介导迟发型变态反应 ,TH2主要分泌 IL- 4、IL- 5 ,主要介导体液免疫应答…     

小剂量白细胞介素-18联合白细胞介素-10在早期Ⅱ型胶原诱导性关节炎中的作用研究

目的 研究不同细胞因子微环境下,白细胞介素(IL)-18对早期Ⅱ型胶原诱导性关节炎(CIA)的作用.方法 CIA小鼠,发病前给予每只重组小鼠腹腔注射IL-18(0.2 μg/d)/IL-10(0.1 μg/d),IL-18(0.2 μg/d)IL-4(0.1 μg/d),IL-18(0.2 μg/d)IL-12(0.1 μg/d),连用5 d,用关节炎评分评估关节炎病情的进展,用反转录聚合酶链反虚(RT-PCR)测定CIA小鼠髌骨及邻近滑膜组织的细胞因子表达水平;用组织学染色评价膝关节的软骨和骨破坏.采用Wilcoxon rank检验. 结果 IL-18/IL-4组关节炎评分轻度降低.IL-18/IL-10联合治疗有效抑制了C1A小鼠的关节炎症反应,免疫第38天治疗组关节炎评分(0.12±0.20)显著低于对照组(0.29±0.19,P<0.05),阻止了滑膜部位的炎细胞浸润,防止了软骨的破坏.滑膜和其附近软骨的Th1型细胞因子[IL-18(0.22±0.06),IL-12(0.14±0.05)]与炎症性细胞因子[IL-6(0.22±0.11)]水平与对照组相比显著降低(P<0.05),Th2型细胞因子[IL-10(6.35±0.12),IL-4(3.57±0.13)]水平与对照组相比明显升高(P<05). IL-18R(0.40±0.15)水平明显降低(P<0.05).T-bet水平降低,转录因子(GATA)-3水平(5.7l±0.11)显著升高(P<0.05).结论 IL-18/IL-10免疫干预尚未发病的CIA小鼠,可以抑制Th1型免疫反应,使免疫反应向Th2逆转,其机制可能为下调了IL-18/IL-18R水平,诱导了转录因子GATA-3的表达.     

急性冠状动脉综合征患者白细胞介素-10及白细胞介素-6表达

目的:通过与C反应蛋白(CRP)检测对比,探讨抗炎因子白细胞介素(IL)-10、促炎因子IL-6与急性冠状动脉综合征(ACS)的关系。方法:冠心病患者78例,其中ACS患者40例(ACS组),稳定型心绞痛(SAP组)患者38例,33例胸痛综合征(CPS组)患者作为对照。所有患者均行冠状动脉造影检查。采用ELISA法检测血浆IL-6、IL-10水平,采用免疫比浊法测定CRP值。结果:与SAP组及CPS组相比,ACS组血浆IL-10水平明显降低,IL-6、CRP水平明显升高,差异有统计学意义;SAP组IL-10水平低于CPS组,IL-6、CRP水平高于CPS组,差异无统计学意义;血浆IL-10水平与IL-6及CRP水平呈负相关,IL-6水平与CRP水平正相关。结论:血浆IL-10水平降低和IL-6、CRP值升高对患者ACS的发生有预测价值。抗炎因子和促炎因子水平的失衡是导致斑块不稳定、急性冠状动脉事件发生的重要因素。     

苏木水提物对心脏异位移植大鼠血清I-4和IL-10的影响

目的 观察苏木水提物对心脏异位移植大鼠血清IL-4与IL-10水平的影响，探讨苏木水提物的免疫抑制作用。方法采用大鼠腹腔同种异位心脏移植术，受体来源于SD大鼠，供体Wistar大鼠。实验分为苏木水提物大、小剂量（生药37．5、25．0g／kg）组，环孢素A（5．0mg／kg）组，苏木（25．0g／kg）＋环孢素A（2．5g／kg）组，对照组（移植心脏但不给药）。术前2d给药，术后3、7d采血测定血清IL-4、IL-10水平，取大鼠心脏观察移植心脏心肌病理变化。结果与对照组比较，大剂量苏木仅在术后3d时，能减轻心脏的排斥反应（t=3．006，P＜0，01）。苏木＋环孢素A合用，术后3d和7d时，都能降低心脏的排斥反应（t=3．009，P＜0．01；t=2．524，P＜0．05），同时血清IL-4低于对照组（t=2．192，P＜0．05；t=2．997，P＜0．01）。苏木大剂量组血清IL-4仅是在7d时，较对照组降低（t=2，988，P＜0．05）。与对照组相比，环孢素A组和苏木小剂量组在术后7d时，血清IL-10水平升高（t=2．198、t=2．173，P＜0．05），苏木大剂量组仅在术后3d时IL-10升高（t=2．203，P＜0．05）；结论 苏木水提物可降低心脏移植后血清IL-4水平，升高移植后血清IL-10的水平，有较强的免疫抑制作用，是一种很有前途的心脏移植后免疫抑制剂。     

亚健康状态老年人血白细胞介素18和白细胞介素10水平变化及意义

目的探讨白细胞介素18（IL-18）、白细胞介素10（IL-10）在老年亚健康状态发生发展中的作用及相互关系。方法应用酶联免疫吸附法测定45例亚健康状态老年人（≥60岁）血浆IL-18和血清IL-10水平,以42例亚健康状态老年前期（50～59岁）和30例健康老年人（≥60岁）作比较,分析它们之间的关系。结果亚健康状态老年人血浆IL-18水平显著高于健康老年人（P〈0.01）,以及亚健康老年前期组（P〈0.05）,血清IL-10水平则显著低于对照组（P〈0.01）和老年前期组（P〈0.05）;血浆IL-18水平与血清IL-10水平之间呈负相关（r=-0.882,P〈0.01）。结论 IL-18和IL-10可能共同构成一局部网络,并可能参与亚健康状态的发生发展过程。     

白细胞介素10与病毒性肝炎

一、白细胞介素１０概述白细胞介素１０（ｉｎｔｅｒｌｅｕｋｉｎｌｏ，ＩＬ－１０），主要由Ｔｈ。细胞产生，但Ｔｈｏ细胞、Ｔｈｌ细胞、ＣＤＳ"Ｔ细胞、活化的Ｂ细胞、单核一巨噬细胞、ＫｕｐＨｅｒ细胞、肝细胞、角化细胞等也可产生［１］。ＩＬ-１０是一种单链糖蛋白，其ｃＤＮＡ序列中含有一编码１７８个氨基酸的开放读码框；切去Ｎ端１８个氨基酸构成的信号肽序列后，成为成熟的含１６０个氨基酸的肽链；在非糖基化状态下，其相对分子质量约１８．７Ｘ１０３，ｐｌｓｔｉｌｌＩＬ－１０参与抑制细胞台成炎性因子、集落刺激因子（ＣＳ…     

免疫治疗在心血管疾病中的应用现状

本文综述了免疫治疗在心血管疾病中的应用近况，包括心脏移植术后的排异反应，外周血管病，慢性充血性心力衰竭，扩张型心肌病，急性病毒性心肌炎，急性风湿热等心血管并发症。     

Prediction of Rejection in Renal Transplantation by Immune Parameters

Background: Monitoring of phenotypic characteristics of T-lymphocytes in peripheral blood is commonly performed to give the clinical parameters in the management of kidney transplant recipients.   Objective: To predict rejection in renal transplantation by immune parameters. Methods: 16 non-diabetic kidney transplant candidates (4 females and 12 males, age = 20-65 yr, first time transplant) were selected. The transplanted patients were divided into two groups based on the rejection during 3 weeks post transplant: group I (n = 9) without rejection and group II (n = 7) with a rejection episode. Immune parameters including lymphocytes subpopulations (by flowcytometry) and immunoglobulin classes (IgM, IgG, IgA and IgE by nephlometric assay) before and 45 days after transplantation were determined.   Results: The results of this investigation showed that the level of immunoglobulin IgG, IgM, IgA and IgE decreased post transplantation due to immunosuppressive drugs. CD3, CD4, CD8 T cells count, CD56 NK cells count and CD20 B cells count pre- and post-transplantation did not show any significant differences. The amount of IgE (220   vs. 462 IU/ml), CD3 (62% vs. 69.7%) and CD4 (35% vs. 41.3%) cells increased in group II during rejection episode pre-transplantation. In addition, IgA increased pretransplantation in group I those without rejection episode in comparison with group II with a rejection episode. Forty five days post transplantation IgA (209   vs. 152 mg/dl), IgG (1009 vs. 703 mg/dl) and CD20 (15%   vs. 10%) increased in group I patients. Conclusion: It is suggestive that pre-transplantation increases IgE, CD3 and CD4 are predictive of acute rejection.     

白介素-10与炎症性肠病

炎症性肠病(inflammatory bowel disease,IBD)是一种慢性、复发性的肠道炎症疾病,其明确病因目前仍不清楚.肠道免疫功能异常导致过量炎症因子释放损伤肠道黏膜在IBD发病中起着关键作用,应用免疫抑制剂减少炎症因子的释放也被应用于IBD的治疗.近年来利用细胞因子调节机体免疫功能以治疗IBD的研究日渐...     

Monitoring of Acute Rejection after Orthotopic Heart Tranplantation

Objectives To study the monitoring of rejection after orthotopic heart thansplantation. Methods From 1998 to 2005, 10 othotopic heart thansplans were performed, and acute rejection was monitored by endomyocardial biopsy as well as by clinical features, ECG, ultrasonocardiography and blood serum determination of Tropin I, and by the combination of these methods, we analysed the monitoring of acute rejection after the heart transplantation. Results With the combination of clinical features, ECG, ultrasonocardiography and blood serum test, 5 occurences of acute rejection were judged in the postoperative course, which were comfirmed by endomyocardial biopsy to be 2 acute rejections inⅠb degree, 3 acute rejections inⅢa degree. Endomyocardial biopsy were routinely performed 21 times postoperatively in which there were 1 acute rejection inⅠa degree and 5 acute rejections inⅠb degree. Conclusions Acute rejection is an important factor influencing the postoperative course of heart transplantation, so it is imperative to have an in-time, effective and planned monitoring procedure for acute rejection. Endomyocardial biopsy is a sensitive and reliable method in diagnosis of acute rejection, but it is invasive and probable for some complications. The noninvasive method such as clinical features, ECG, ultrasonocardiography and blood serum test can be used as additive means in the diagnosis of acute rejection. Endomyocardial biopsy should be combined with some noninvasive methods in monitoring acute rejection after the heart transplantation.     

Investigation of Interleukin-10 Promoter Polymorphisms and Interleukin-10 Levels in Children with Irritable Bowel Syndrome

Background/Aims

The aim of this study was to investigate whether genetic variations at positions -1082, -819, and -592 in the interleukin (IL)-10 promoter affect IL-10 production in children with irritable bowel syndrome (IBS).

Methods

Ninety-four children with IBS and 102 children as healthy controls (HCs) were enrolled. Genomic DNA was extracted, and IL-10 -1082, -819, and -592 polymorphisms were detected by direct sequencing from all participants. Peripheral blood mononuclear cells (PBMCs) from 46 IBS children and 38 HCs were isolated and cultured with and without 5 ng/mL Escherichia coli lipopolysaccharide (LPS). IL-10 levels in the culture supernatants were measured by enzyme-linked immunosorbent assay.

Results

There were no significant differences in the distribution of IL-10 -1082, -819, and -592 polymorphisms or in the allele and haplotype frequencies between IBS children and HCs. PBMCs from children with IBS had significantly lower IL-10 levels after LPS stimulation than PBMCs from HCs (p=0.011); however, LPS-induced IL-10 levels in PBMCs with different genotypes of -819 and -592 polymorphisms were not significantly different between IBS patients and HCs.

Conclusions

Although significantly lower LPS-induced IL-10 production by PBMCs was noted, it is unlikely that IL-10 production was fully genetically determined in our IBS children. ClinicalTrials.gov identifier: NCT01131442.     

Overview of immunosuppression in liver transplantation

Continued advances in surgical techniques and immunosuppressive therapy have allowed liver transplantation to become an extremely successful treatment option for patients with end-stage liver disease. Beginning with the revolutionary discovery of cyclosporine in the 1970s, immunosuppressive regimens have evolved greatly and current statistics confirm one-year graft survival rates in excess of 80%. Immunosuppressive regimens include calcineurin inhibitors, anti-metabolites, mTOR inhibitors, steroids and antibody-based therapies. These agents target different sites in the T cell activation cascade, usually by inhibiting T cell activation or via T cell depletion. They are used as induction therapy in the immediate peri- and post-operative period, as long-term maintenance medications to preserve graft function and as salvage therapy for acute rejection in liver transplant recipients. This review will focus on existing immunosuppressive agents for liver transplantation and consider newer medications on the horizon.     

白细胞介素-10与肝脏疾病

肝脏疾病与各种因素导致的肝脏急、慢性损伤关系密切,而炎症反应和机体的免疫调节机制在各种肝病的发生和发展中具有举足轻重的作用.白细胞介素-10是一种重要的炎症负性调控因子,参与多种疾病的病理生理调节过程,对各种肝脏疾病均有一定的影响.     

白介素-8和白介素-10在动脉粥样硬化斑块形成中的相互作用

近年来病理学研究已证实,急性冠脉综合征(acute coronary syndrome,ACS)的急性心肌缺血事件不仅是由于动脉粥样硬化(AS)的发展,更重要的是冠状动脉内不稳定斑块的存在,继而发生了痉挛、破裂、出血和血栓形成.急性心肌梗死(acute myocardial infarction,AMI)可以引起炎症反应的各个表现,其主要表现为中性粒细胞的积聚、黏附及浸润,活化的巨噬细胞(Macrophages,mφ)产生大量细胞因子、生长因子、某些蛋白酶及反应性氧化自由基,后者能进一步氧化低密度脂蛋白分子,形成氧化修饰低密度脂蛋白而损伤内皮.     

白介素-10在肺移植中的研究进展

肺移植术后的并发症主要包括缺血再灌注损伤、急性排异反应和感染、闭塞性细支气管炎为表现的慢性同种异体移植物功能障碍。白介素-10是一个多效应的细胞因子,可通过抑制炎性免疫反应和T细胞介导的免疫反应,对减轻肺移植术后的并发症有一定的作用。     

白介素10与慢性阻塞性肺疾病

白介素10(interleukin-10,IL-10)是一个有力的抗炎和免疫调节因子,在体内炎症反应过程中发挥着重要的作用.它通过抑制炎症因子和细胞趋化因子的合成、抑制细胞介导的免疫反应,从而起到抗炎和免疫调节作用.本文就IL-1O的分子生物学特点、细胞分布及IL-1O与慢性阻塞性肺疾病的关系作一综述.