用单层辐射人羊胎治疗鼠烫伤创面的实验研究

为了探讨单层辐射人羊膜对模型鼠烫伤创面敷盖治疗的疗效及愈合各阶段创面的组织学变化，我们观察了４０只Ｗｉｓｔａｒ鼠深Ⅱ度烫伤创面。将单层辐射人羊膜和油纱对照贴敷创面进行观察，采用了活杀取材及电镜扫描检查法。结果表明，模型鼠创面用人羊膜贴敷侧比对照侧提前愈合。而且愈合创面外观是毛发规则丛生，皮肤具有弹性，表皮各层均为正常皮肤组织像。经动物实验，我们认为此无免疫排斥现象，无毒、无菌，无刺激性。而且能防止     

人羊膜生物敷料对鼠烫伤创面保护作用的疗效观察

目的:研究单层辐射人羊膜对模型鼠烫伤创面敷盖治疗的疗效及愈合各阶段创面的组织学变化.方法:观察了 40只Wistar鼠深Ⅱ°烫伤创面.将单层辐射人羊膜和油纱对照贴敷创面进行观察,采用了活杀取材及电镜扫描检查法.结果:模型鼠创面用人羊膜贴敷侧比对照侧提前愈合.而且愈合创面外观是毛发规则丛生,皮肤具有弹性,表皮各层均为正常皮肤组织像.结论:经动物实验,此膜无免疫排斥现象,无毒、无菌、无刺激性.而且能防止创面水份、电解质和蛋白质的丢失,增强模型鼠的抵抗能力,避免感染,加速愈合.是治疗鼠烫伤创面的良好敷料.     

单层人羊膜对颜面创面保护作用的临床观察

目的：为了探讨经γ射线辐射的单层人羊膜作为生物敷料用于颜面创伤面的临床治疗的有效保护作用。方法：我们对颜面105个创伤创面对照贴敷人羊膜和油纱条，进行临床观察治疗。结果：我们发现颜面浅Ⅱ度创面用人羊膜治疗侧比用油纱条治疗侧平均提前愈合4天－5天，颜面深II度创面用人羊膜治疗侧比用油纱条治疗侧平均提前愈合5天－6天。结论：我们认为将新鲜人羊膜去除绒毛膜后，再将羊膜层干燥，修剪成型，制成不同规格的单层薄膜，再经γ射线辐射而成的单层辐射人羊膜（简称羊膜），在用于颜面创伤创面的临床治疗时，在防止创面感染，缩短创面的愈合时间，降低颜面瘢痕畸形的发生，保护颜面皮肤的正常弹性和美观等诸多方面都比使用油纱条要优越，有效，具有良好的颜面创伤创面保护作用和治疗作用。     

单层人羊膜对颜面创面保护作用的临床观察

目的:为了探讨经γ射线辐射的单层人羊膜作为生物敷料用于颜面创伤创面的临床治疗的有效保护作用。 方法我们对颜面105个创伤创面对照贴敷人羊膜和油纱条,进行临床观察治疗。 结果 我们发现颜面浅Ⅱ度创面用人羊膜治疗侧比用油纱条治疗侧平均提前愈合4天-5天;颜面深Ⅱ度创面用人羊膜治疗侧比用油纱条治疗侧平均提前愈合5天-6天。 结论我们认为将新鲜人羊膜去除绒毛膜后,再将羊膜层干燥,修剪成型,制成不同规格的单层薄膜,再经γ射线辐射而成的单层辐射人羊膜(简称羊膜),在用于颜面创伤创面的临床治疗时,在防止创面感染,缩短创面的愈合时间,降低颜面瘢痕畸形的发生,保护颜面皮肤的正常弹性和美观等诸多方面都比使用油纱条要优越、有效,具有良好的颜面创伤创面保护作用和治疗作用。     

用单层辐射人羊膜治疗取皮区及擦皮创面的临床应用研究

为了探讨单层辐射人羊膜在取皮区及浅、深度擦皮创面上应用的临床疗铲及其对创面的保护作用，我们用羊膜和常规油纱分别贴静处述筘个创面的两部分进行治疗对照观察。结果表明，用人间膜 的创面比用油纱侧的创面平均提前４￣５天愈合。而且用间膜侧的创面无１例感染。提示人羊膜对上述创面的早期愈合起到良好的治疗作用，并且对预防创面感染起到保护作用单层辐人羊膜是可在临床应用的良好的创面覆盖物。     

用复层辐射人羊膜治疗取皮区及擦皮创面的临床应用研究

目的 探讨复层辐射人羊膜在取皮区及浅、深度擦皮创面上应用的临床疗效及其对创面的保护作用.方法 用羊膜和常规油纱分别贴敷上述各个创面的两部分进行治疗.对照观察.结论 用人羊膜侧的创面比用油纱侧的创面平均提前4-5天愈合.用羊膜侧的创面无1例感染.结论人羊膜对刨面的早期愈合起到良好的治疗作用,并且对预防创面感染起到保护作用.复层辐射人羊膜是可在临床应用的良好的创面覆盖物.     

重组人结缔组织生长因子(rhCTGF)促进大鼠烫伤创面愈合的实验研究

目的通过结缔组织生长因子(CTGF)的局部应用,观察其对烫伤创面愈合的促进作用。方法大鼠随机分为用药组和对照组,复制深Ⅱ度烫伤模型,每组为自体对照,用药物和基质,烫伤次日起,开始给药并对创面拍照,观察创面结痂时、创面完全愈合时分别取愈合组织做光镜、电镜、细胞周期检测。结果①给药第15天中剂量(30ng)、高剂量(100ng)组受试侧创面相对愈合面积与同组对照侧差异显著(P<0. 05);②创面完全脱痂时取组织,光学显微镜观察并评分, 10ng组受试侧与对照侧相比差异显著(P<0. 05);③电子显微镜观察,各剂量组受试侧纤维排列明显较对照侧更近似于正常皮肤;④流式细胞仪检测结果显示,中剂量组、高剂量组受试侧细胞G1期较对照侧短,并有差异显著性(P<0. 05 )。结论局部应用合适剂量CTGF可促进深Ⅱ度烫伤创面愈合,缩短创面愈合时间。     

复层辐射人羊膜应用于烧伤创面的研究

目的　寻找较为理想的烧伤创面覆盖物。方法　 1994年以来 ,我们对复层辐射人羊膜进行了实验及临床研究。结果　表明它除了具有透明度及柔韧性外 ,还具有比单层羊膜更大的强度 ,与临床常用的断层猪皮相同的透水、透气性及与自体皮相近的创面粘合力。结论　复层辐射人羊膜覆盖烧伤创面是一种理想的生物敷料。     

多层羊膜移植治疗持续性角膜溃疡23例临床分析

目的 探讨多层羊膜移植对长期不能愈合修复的角膜溃疡的治疗效果。方法 23只眼中病原体不明者15例，复发性病毒感染者8例，对角膜溃疡波及深度小于1/2角膜厚度者行1层羊膜移植或填塞，波及深度大于1/2或后弹力层膨突者，行1层或多层羊膜填塞联合1层羊膜移植，所有病例均外层用单层羊膜做角膜缘-全角膜贴敷。结果 23例炎症及感染均得到迅速有效控制，两周左右溃疡愈合。平均随访半年均获得稳定的眼表，未见复发，无或有少量新生血管，视力均得到不同程度提高。结论 多层羊膜移植治疗持续性角膜溃疡，是一种有价值的治疗方法。具有缩短病程，降低复发率等优点。     

烧烫伤软膏对实验性小鼠、大鼠烧烫伤的作用

目的:观察烧烫伤软膏促进小鼠烧烫伤及大鼠烫伤创面愈合的作用。方法:①小鼠造成背部Ⅲ°烫伤,分别涂抹烧烫伤软膏、京万红软膏、凡士林,观察创面愈合天数,并测量给药后不同时间创面的面积。②小鼠造成背部Ⅲ°烧伤,用药后观察创面愈合天数及毛生长百分率。③大鼠造成背部Ⅲ°烫伤,用药后观察创面愈合天数及毛生长百分率。结果:烧烫伤软膏与京万红软膏作用相近。结论:烧烫伤软膏对小鼠烧烫伤和大鼠烫伤有治疗作用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.