Grape seed extract and zinc containing nutritional food supplement prevents onset and progression of age-related cataract in Wistar rats

Objective

To study possible inhibition of oxidative stress and cataract formation by single combined formulation of grape seed extract and Zincovit tablets against sodium selenite-induced age-related cataract in Wistar rat pups.

Methods

Oxidative stress and consequent cataract formation was induced by subcutaneous administration of a single dose of sodium selenite (10 µmoles/kg) to Wistar rat pups on day 7 post-natally. In experiments designed to inhibit such cataract formation, the pups were pretreated subcutaneously with combined formulation of grape seed extract and Zincovit tablets (40, 80 and 160 mg/kg), one day prior to the administration of selenite and continuing such treatment till day 20, when the experiments were terminated. The extent of tissue damage caused by the selenite was assessed biochemically by measurements of the levels of reduced glutathione, glutathione peroxidase, glucose-6-phosphate dehydrogenase, protein thiol, catalase, superoxide dismutase, malondialdehyde, aldose reductase, sorbitol dehydrogenase and adenosine triphosphate in the isolated lenses. Cataract formation and its prevention were monitored by examining the eye with pen light illumination and subsequent photography of the isolated lenses.

Results

Injection of selenite led to a significant loss of lens clarity due to cataract formation. In the group treated with combined formulation of grape seed extract and Zincovit tablets, the formation of cataract was significantly prevented. In the normal and selenite induced senile cataract control group, the levels of lens oxidative stress markers, G6PD and ATP were substantially lower than in the grape seed extract with Zincovit tablets treated group (p < 0.05).

Conclusion

Over all, the results suggest that single combined formulation of grape seed extract and Zincovit tablets may offer a prophylactic measure against onset and progression of age- related cataract of human subjects as nutritional food supplement.     

葡萄籽提取物对乙醇所致小鼠急性肝损伤保护作用的研究

目的研究葡萄籽提取物对乙醇所致小鼠急性肝损伤的保护作用。方法取雄性昆明种小鼠50只,随机分为阴性对照组、急性肝损伤模型组和不同剂量的葡萄籽提取物试验组。试验组给予不同剂量的葡萄籽提取物灌胃,实验末采用50%乙醇经口一次灌胃建立小鼠急性肝损伤模型。检测肝组织中丙二醛（MDA）、还原型谷胱甘肽（GSH）和甘油三酯（TG）的含量,并对小鼠肝脏取材,进行病理组织学检测。结果葡萄籽提取物高剂量组动物的肝脏TG含量明显低于模型对照组,GSH含量明显高于模型对照组,差异有统计学意义（P〈0.05或P〈0.01）。病理学检测结果显示高剂量组动物的肝脏脂变程度明显低于模型对照组,差异有统计学意义（P〈0.01）。结论葡萄籽提取物对乙醇所致小鼠急性肝损伤具有一定的保护功能。     

葡萄籽提取物抗实验性动脉粥样硬化作用及其机理研究

为探讨葡萄籽提取物抗实验性动脉粥样硬化作用及机理 ,将 5 0只动脉粥样硬化敏感株近交系C5 7BL 6J小鼠 ,随机分为正常对照组、高脂模型组、葡萄籽提取物低、高剂量组和药物对照组 ,正常对照组和高脂模型组 ,每日分别给予普通饲料和高脂饲料 ,葡萄籽提取物组和药物对照组 ,在每日给予高脂饲料的同时 ,分别添加葡萄籽提取物 (0 2mg g、0 6mg g体重 )和普托罗考片 (0 2mg g体重 ) ,实验至第 2 1周末 ,眼球放血处死小鼠 ,检测血清氧化型低密度脂蛋白 (OX LDL)、细胞粘附因子 1(ICAM 1)和一氧化氮 (NO)含量 ,同时取主动脉作病理形态学观察。结果发现 ,葡萄籽提取物组 ,血清OX LDL和ICAM 1水平均高于正常对照组 ,但明显低于高脂模型组 ,血清NO水平 ,则低于正常对照组而高于高脂模型组。观察主动脉部位的形态显示 ,葡萄籽提取物组其血管壁增厚程度、玻璃样改变和泡沫细胞数量均轻于模型组 ,高剂量组效果为佳 ,但仍重于正常对照组。实验结果提示 ,葡萄籽提取物具有减缓C5 7BL 6J小鼠动脉粥样硬化形成的作用 ,这可能与阻抑OX LDL、ICAM 1含量升高减低对血管内皮细胞损伤及保护血管内皮功能等作用有关     

Protective effect of red wine on oxidative stress and antioxidant enzyme activities in the brain and kidney induced by feeding high cholesterol in rats

The effect of red wine (400 ml/70 kg) on brain and kidney oxidative stress and antioxidative enzymes activities induced by cholesterol-enriched diet (supplemented with 1.65% of cholesterol (w/w) for 4 weeks) was studied in rats. When red wine (Montilla-Moriles, Cordoba, Spain) was simultaneously supplemented to high-cholesterol diet for 4 weeks, total cholesterol and lipid peroxidation products in the brain, kidney and erythrocytes significantly decreased compared with the high-cholesterol, while GSH content and antioxidative enzymes activities enhanced. On the other hand, the urinary excretion of urea, creatinine and albumin decreased significantly. These results suggest that red wine may have a neuro-nephroprotective effect against oxidative stress and hypercholesterolemia.     

The effect of grape seed extract on cardiovascular risk markers: a meta-analysis of randomized controlled trials

Recent animal studies have suggested that grape seed extract has beneficial effects on the cardiovascular system. Randomized trials in human beings have yielded conflicting results. The objective of this systematic review was to assess the effect of grape seed extract on changes in blood pressure, heart rate, lipid levels, and C-reactive protein (CRP) levels. We searched MEDLINE (January 1, 1950, through October 31, 2010), Agricola (January 1, 1970, through October 31, 2010), Scopus (January 1, 1996, through October 31, 2010), and the Cochrane Central Register of Controlled Trials (through October 31, 2010) for randomized controlled trials in human beings of grape seed extract reporting efficacy data on at least one of the following end points: systolic or diastolic blood pressure, heart rate, total cholesterol, low-density or high-density lipoprotein cholesterol, triglycerides, or CRP. A manual search of references from primary and review articles was performed to identify additional relevant trials. For all endpoints except CRP, the mean change in each parameter from baseline was treated as a continuous variable and the effect size was calculated as the weighted mean difference between the means in the grape seed extract and control groups. Data on CRP were pooled as a standardized mean difference. Nine randomized, controlled trials (N=390) met the inclusion criteria, and a meta-analysis was conducted. Upon meta-analysis, grape seed extract significantly lowered systolic blood pressure (weighted mean difference −1.54 mm Hg (95% confidence interval −2.85 to −0.22, P=0.02]), and heart rate (weighted mean difference −1.42 bpm (95% confidence interval −2.50 to −0.34, P=0.01]). No significant effect on diastolic blood pressure, lipid levels, or CRP was found. No statistical heterogeneity was observed for any analysis (I²<39% for all). Egger's weighted regression statistic suggested low likelihood of publication bias in all analysis (P>0.05 for all), except for the effect on diastolic blood pressure (P=0.046). Based on the currently available literature, grape seed extract appears to significantly lower systolic blood pressure and heart rate, with no effect on lipid or CRP levels. Larger randomized, double-blinded trials evaluating different dosages of grape seed extract and for longer follow-up durations are needed.     

Therapeutic effects of Teucrium polium extract on oxidative stress in pancreas of streptozotocin-induced diabetic rats

Increased oxidative stress is a widely accepted factor in the development and progression of diabetes and its complications. In this study, we evaluated the antioxidative potential of Teucrium polium (Family Lamiaceae) aqueous extract for protecting rat pancreatic tissue against streptozotocin (STZ)-induced oxidative stress. Diabetes was induced in rats by intraperitoneal injections of at a single dose of STZ at 40 mg/kg. The crude extract (equivalent to 0.5 g of plant powder/kg of body weight) was administered orally (intragastrically) to a group of STZ diabetic rats for 30 consecutive days. Changes in antioxidant status were evaluated by determining catalase (CAT) and superoxide dismutase (SOD) activities and the level of reduced glutathione (GSH) in pancreatic tissue. In addition, serum nitric oxide (NO) concentration, pancreatic tissue malondialdehyde (MDA) (an index of lipid peroxidation) level, and reliable markers of protein oxidation such as protein carbonyl content (PCO) and advanced oxidation protein products (AOPP) were also determined. Under diabetic conditions, blood glucose level, serum NO concentration, and pancreatic MDA, PCO, and AOPP levels were all increased. The diabetic rats also exhibited pancreatic GSH depletion along with significant reductions in activities of CAT and SOD. Rats treated with T. polium extract had significantly higher GSH levels along with enhanced CAT and SOD activities in pancreatic tissue. In addition to suppressed blood glucose levels, serum NO, pancreatic MDA, PCO, and AOPP levels were all lower than in the diabetic group. Our results strongly support the proposal that antioxidative activity of T. polium occurs by quenching the extent of lipid and protein oxidation. Based on these observations, it is concluded that T. polium may have protective effect(s) on pancreatic tissue in STZ-induced oxidative stress due to its high antioxidative potential.     

The neuroprotective effects of an ethanolic turmeric (Curcuma longa L.) extract against trimethyltin-induced oxidative stress in rats

Introduction: Oxidative stress is known to contribute to the pathogenesis of neurodegenerative disorders. An ethanolic turmeric (Curcuma longa L.) extract containing curcumin has been reported to produce antioxidant effects.

Objective: The present study aims to investigate the possible neuroprotective effects of the ethanolic turmeric extract against trimethyltin (TMT)-induced oxidative stress in Sprague Dawley rats.

Methods: The ethanolic turmeric extract and citicoline were administered to the TMT exposed rats from day 1 to day 28 of the experiment. The TMT injection was administered on day 8 of the experiment. The plasma and brain malondialdehyde (MDA) and reduced glutathione (GSH) levels, and the activities of the superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) enzymes in the brain were examined at the end of the experiment.

Results: The administration of 200 mg/kg bw of the ethanolic turmeric extract prevented oxidative stress by decreasing the plasma and brain MDA levels and increasing the SOD, CAT, and GPx enzyme activities and GSH levels in the brain. These effects seem to be comparable to those of citicoline.

Discussion: The ethanolic turmeric extract at a dose of 200 mg/kg bw may exert neuroprotective effects on TMT-exposed Sprague Dawley rats by preventing them from oxidative stress.     

Efficacy of Bilberry and Grape Seed Extract Supplement Interventions to Improve Glucose and Cholesterol Metabolism and Blood Pressure in Different Populations—A Systematic Review of the Literature

Intervention with fruit extracts may lower glucose and lipid levels, as well as blood pressure. We reviewed the efficacy of bilberry and grape seed extracts to affect these outcomes across populations with varying health status, age and ethnicity, across intervention doses and durations, in 24 intervention studies with bilberry and blackcurrant (n = 4) and grape seed extract (n = 20). Bilberry and blackcurrant extract lowered average levels of glycated hemoglobin (HbA1c), at least in Chinese subjects, especially in those who were older, who were diagnosed with Type 2 Diabetes Mellitus (T2DM) and who were participating in longer-term studies. We also found good evidence that across studies and in subjects with hypercholesterolemia, T2DM or metabolic syndrome, intervention with bilberry and blackcurrant extract, and to some extent grape seed extract, significantly lowered total and low density lipoprotein (LDL) cholesterol levels after four weeks. Intervention with grape seed extract may reduce systolic and diastolic blood pressure in subjects with hypertension or metabolic syndrome. Differential responsiveness in cholesterol and blood pressure outcomes between stratified populations could not be explained by age, dose or study duration. In conclusion, bilberry and blackcurrant extract appears effective in lowering HbA1c and total and LDL cholesterol, whereas grape seed extract may lower total and LDL cholesterol, and blood pressure, in specific population groups.     

葡萄籽提取物对小鼠血脂水平的影响

为探讨葡萄籽提取物对高脂膳食小鼠血脂水平的影响。将 4 0只小鼠随机分为正常对照组 (饲普通饲料 ) ,高脂模型组 (饲高脂饲料 )和葡萄籽提取物低、高剂量组 (饲高脂饲料的同时加饲葡萄籽提取物 (0 .2、0 .6 mg/ g体重 ) ,实验至 2 0周 ,眼球取血测定血清 TC、TG、MDA含量。结果表明 ,葡萄籽提取物组血清 TC、TG、MDA升高的幅度较高脂模型组明显减少。提示葡萄籽提取物对高脂膳食致小鼠血脂升高有一定的阻抑作用。     

葡萄籽提取物对小鼠动脉粥样硬化的作用

目的 :　探讨葡萄籽乙醇提取物对已形成的小鼠动脉粥样硬化 (AS)病变的消退作用。方法 :　采用 AS敏感株近交系 C5 7BL/6 J小鼠 ,经致 AS饲料饲喂 2 0 w复制 AS模型 ,然后改用普通饲料 ,同时给予葡萄籽提取物灌胃干预 4w,于 2 5 w末 ,眼球放血处死小鼠 ,并取主动脉作病理切片 ,观察血脂及形态学变化。结果 :　经致 AS饲料饲喂 2 0 w的小鼠 ,主动脉部位已形成了含有大量泡沫细胞的早期 AS病变 ,主动脉损伤评分为 7.4± 1 .3 ,且血清甘油三酯、胆固醇和丙二醛含量大幅度升高。经给予葡萄籽提取物灌胃 ,小鼠主动脉损伤较模型组明显减轻 ,主动脉损伤评分为 6 .4± 1 .2 ,血清甘油三酯、胆固醇和丙二醛含量均明显下降 ,高剂量组效果为佳。结论 :　葡萄籽提取物具有减轻已形成的动脉粥样硬化早期病变的作用 ,且能使血清甘油三酯、胆固醇和丙二醛含量下降 ,显示有明显的抗动脉粥样硬化效应     

Red wine consumption prevents vascular oxidative stress induced by a high-fat meal in healthy volunteers

In order to investigate the effect of red wine on plasma lipid and oxidative stress parameters after a high-fat meal, fifteen healthy volunteers were studied: three days after a high-fat meal with 250 mL of water, they received the same meal with 250 mL of red wine. During both periods, serial blood samples were drawn before and 2, 4, and 8 hours after the meal to evaluate plasma lipids (cholesterol and triglycerides; retinyl palmitate), oxidative stress (D-ROM, and malondialdehyde) and antioxidant (total plasma antioxidant levels and uric acid) parameters. During the meal without wine, plasma lipid parameters increased significantly, whereas plasma total plasma antioxidant levels decreased, and a trend toward reduction of uric acid levels was seen). A similar trend in lipid parameters was observed after the meal with wine; no significant difference in individual lipid parameter trends after a meal with and without wine was observed. Wine ingestion induced higher total plasma antioxidant levels and uric acid; malondialdehyde levels remained constant after wine ingestion. Plasma D-ROM showed a significant postprandial increase in both experiments, but it was significantly lowered after wine ingestion. Our results give evidence of oxidative stress following a fat-rich meal in healthy subjects, suggesting that ingestion of red wine during a high-fat meal significantly reduces oxidative stress without inducing any significant modification in postprandial lipemia.     

Grape powder polyphenols attenuate atherosclerosis development in apolipoprotein E deficient (E0) mice and reduce macrophage atherogenicity

The beneficial health effects of red wine have been attributed to the antioxidant activity of its polyphenols. The present study investigated the effects of a standardized freeze-dried powder made from fresh grapes, rich in grape-specific polyphenols and free of alcohol, on oxidative stress, atherogenicity of macrophages, and the development of atherosclerotic lesions in apolipoprotein E deficient (E(0)) mice. Thirty E(0) mice were assigned to 3 groups. Mice consumed water alone (control), 150 mug total polyphenols/d in the form of grape powder (grape powder), or the equivalent amount of glucose and fructose (placebo) in drinking water for 10 wk. Consumption of grape powder reduced the atherosclerotic lesion area by 41% (P < 0.0002) compared to the control or placebo mice. The antiatherosclerotic effect was at least partly due to a significant 8% reduction in serum oxidative stress, an up to 22% increase in serum antioxidant capacity, a significant 33% reduction in macrophage uptake of oxidized LDL, and a 25% decrease in macrophage-mediated oxidation of LDL relative to controls. Grape powder directly protected both plasma LDL and macrophages from oxidative stress in vitro. We conclude that polyphenols from fresh grape powder directly affect macrophage atherogenicity by reducing macrophage-mediated oxidation of LDL and cellular uptake of oxidized LDL. Both of these processes can eventually reduce macrophage cholesterol accumulation and foam cell formation and hence attenuate atherosclerosis development.     

Attenuation of renal ischemia-reperfusion injury by proanthocyanidin-rich extract from grape seeds

The effects of proanthocyanidin-rich extract in rats subjected to renal ischemia-reperfusion were examined. Proanthocyanidin-rich extract, which is prepared from grape seeds (Vitis vinifera L.), was given orally at doses of 5 and 10 mg/kg body weight/d for 20 consecutive days prior to ischemia-reperfusion. Administration of proanthocyanidin-rich extract attenuated renal dysfunction, as indicated by serum urea nitrogen and creatinine levels. Additionally, in the ischemic-reperfused kidneys, increased levels of thiobarbituric acid (TBA)-reactive substance and alterations of antioxidant enzyme activities such as superoxide dismutase, catalase and glutathione peroxidase (GSH-Px) were observed. Proanthocyanidin-rich extract-treated groups showed significantly reduced renal TBA-reactive substance levels and enhanced catalase and GSH-Px activities. These results suggest that proanthocyanidin-rich extract has protective effects against ischemia-reperfusion-induced renal damage associated with oxidative stress.     

Effects of short-term moderate alcohol administration on oxidative stress and nutritional status in healthy males

The effects of moderate amounts of different alcoholic beverages on oxidative stress and nutritional parameters were investigated in 40 healthy subjects. Ethanol 40 g/day was administered at the two main meals for 30 days by beer (group A), wine (group B) or spirit (group C); controls (group D) maintaned abstinence. Malondyaldeide (MDA), adenosine-triphosphate (ATP), reduced-glutathione (GSH), E-vitamin and nutritional status were evaluated at the start (T0) and the end (T1) of the study. At T1 controls did not present significant changes in the assessed parameters, while a significant increase of malondyaldeide (MDA) and a significant decrease of reduced-glutathione and E-vitamin in group A, B and C and of ATP in group C were observed. Fat mass (FM) increased slightly in group A and B and decreased in group C. Ethanol decreased antioxidant parameters and increased lipoperoxidation parameters. However some of these changes appeared attenuated when ethanol was consumed in beer or wine. Finally, short-term moderate ethanol intake appeared to influence the FM, although it was not able to significantly affect nutritional or body composition.     

Health effects in children aged 3-6 years induced by environmental lead exposure

OBJECTIVES: To investigate the involvement of oxidative damage in lead-induced toxicity in children aged 3-6 years and to enlighten whether oxidative stress indicators are correlated with the known indices of lead toxicity. METHODS: Blood samples were collected from 408 subjects (217 boys and 191 girls) in the urban kindergartens. The age range of the subjects was 3-6 years. Blood lead levels (BLLs) were analyzed by flameless atomic absorption spectrophotometry. Activities of delta-aminolevulinic acid dehydratase (ALAD), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and contents of glutathione (GSH) in erythrocyte and levels of plasma malondialdehyde (MDA) were analyzed spectrophotometrically in these children. RESULTS: Children with BLLs >or = 100 microg/L had significantly decreased erythrocyte ALAD activities and increased plasma MDA levels compared to the children with BLLs < 100 microg/L. No significant changes were observed in erythrocyte SOD and GSH-Px activities and GSH levels associated with elevated BLLs in these children. CONCLUSION: Present data indicate that oxidative damage could be induced by lead in children with BLLs > or = 100 microg/L, and this may partly be attributed to the inhibited ALAD activities. Statistically significant changes of oxidative stress parameters in preschool children while BLLs were more than 100 microg/L could be implicated that oxidative damage might contribute to lead-induced intellectual impairment.     

The effect of supplementation of grape seed proanthocyanidin extract on vascular dysfunction in experimental diabetes

Increased oxidative stress and impaired endothelium-dependent relaxation could underlie many of the vascular complications associated with diabetes. We aimed to investigate the effect of supplementation with grape seed proanthocyanidin extract (GSPE), a natural antioxidant, on vascular responses and oxidative stress in streptozotocin-induced diabetic rats. Male Sprague-Dawley rats were divided into three groups: control rats, untreated diabetic rats, and GSPE (100 mg/kg, for 6 weeks)-supplemented diabetic rats. Thoracic aorta rings of the rats were mounted in organ baths, and relaxant responses to acetylcholine (ACh), A23187, and sodium nitroprusside (SNP) were assayed in tissues precontracted with 60 mM KCl. Plasma samples used for the measurement of malondialdehyde (MDA) level and superoxide dismutase (SOD) activity. The endothelium-dependent relaxations in response to ACh and A23187 were impaired, but endothelium-independent relaxation in response to SNP did not change in diabetic rats. Supplementation with GSPE significantly improved the relaxant responses to ACh and A23187. The MDA level was significantly elevated and the plasma SOD activity was decreased in diabetic rats, but supplementation with GSPE attenuated the elevated MDA levels and increased plasma SOD activity. Thus supplementation of GSPE may attenuate oxidative stress through the inhibition of lipid peroxidation and may restore endothelial function and reduce the risk of vascular disease in diabetes.     

A possible subcellular mechanism underlying the "French paradox": the opening of mitochondrial K(ATP) channels

A reduction in the risk of coronary heart disease has been associated to moderate red wine consumption. We tested whether a nonalcoholic red wine extract would open mitochondrial K(ATP) channels in guinea pig myocytes. The opening of mitochondrial K(ATP) channels was assessed by endogenous flavoprotein fluorescence. Red wine extract (100 μg·mL(-1)) increased flavoprotein oxidation (10.9% ± 1.2%, n = 20). This effect was prevented by the mitochondrial K(ATP) channel blocker, 5-hydroxydecanoate (500 μmol·L(-1); 0.3% ± 1.1%, n = 13), confirming the hypothesis that red wine extract opens mitochondrial K(ATP) channels.     

Effect of red wine and red grape extract on blood lipids, haemostatic factors, and other risk factors for cardiovascular disease

OBJECTIVE: Some epidemiological studies found a lower risk of cardiovascular disease among wine drinkers than among drinkers of other types of ethanol. This difference might be due to an effect of nonalcohol compounds in wine on important cardiovascular risk factors. The objective of this study was to compare the effect of red wine, nonalcohol compounds of red wine and placebo on established cardiovascular risk factors. DESIGN: A parallel, four-armed intervention study. SUBJECTS: A total of 69 healthy 38-74-y-old men and women. INTERVENTIONS: Subjects were randomised to either 1: red wine (males: 300 ml/day, 38.3 g alcohol/day, female subjects: 200 ml/day, 25.5 g alcohol/day), 2: water + red grape extract tablets (wine-equivalent dose), 3: water + red grape extract tablets (half dose), or 4: water + placebo tablets for a period of 4 weeks. No other sources of alcohol or anthocyanin were allowed. Plasma high-density lipoprotein (HDL)-cholesterol (HDL-C), low-density lipoprotein (LDL)-cholesterol (LDL-C), HDL-C/LDL-C-ratio, very-low-density lipoprotein (VLDL)-triacylglycerol, total cholesterol, fibrinogen, factor VII coagulant activity (FVIIc), blood pressure, and body weight were determined before and after intervention. RESULTS: Wine consumption was associated with a significant 11-16% increase in fasting HDL-C and 8-15% decrease in fasting fibrinogen relative to not drinking wine. There were no significant treatment effects on fasting LDL-C, HDL-C/LDL-C-ratio, VLDL-triacylglycerol, total cholesterol, FVIIc, or blood pressure. Drinking wine was associated with relative body weight increments closely corresponding to the energy contributed by the alcohol component. CONCLUSION: Moderate red wine consumption for 4 weeks is associated with desirable changes in HDL-C and fibrinogen compared with drinking water with or without red grape extract. The impact of wine on the measured cardiovascular risk factors thus seems primarily explained by an alcohol effect. Our finding suggests that the putative difference in cardiac risk associated with wine vs other alcoholic beverages might be rather explained by other life-style confounders than by red wine contents of nonalcohol components.     

柴油车尾气颗粒物提取物对V79细胞的毒性

目的 观察柴油车尾气颗粒物提取物(DEPE)对中国仓鼠肺成纤维细胞(V79)增殖、细胞膜完整性及氧化损伤的毒性。方法 DEPE染毒不同浓度或时间后，通过检测V79细胞的细胞生存率、乳酸脱氢酶(LDH)漏出率、谷胱甘肽(GSH)含量、谷胱甘肽过氧化物酶(GSH-PX)活力的变化规律及相互的关系，评价DEPE对细胞的一般毒性和氧化损伤作用。结果 在该实验条件下，DEPE可致细胞生存率降低，半数抑制浓度(IC50)约为6400μ/ml；细胞内LDH漏出率增加，GSH含量下降，并伴随着GSH—Px活力升高。结论 一定剂量下，DEPE可降低细胞生存率，损伤细胞膜，致细胞氧化损伤。