针灸治疗带状疱疹的疗法特点及分析

目的:分析针灸治疗带状疱疹疗法特点及作用。方法:运用文献计量学方法,以中国期刊全文数据库为资料来源,对针灸治疗带状疱疹进行定量分析。结果:检出有效文献157篇,涉及的期刊杂志达76种,完整的针灸治疗Hz临床报道145篇;以临床研究类文献为主;综合疗法为常用治法。结论:针灸疗法治疗带状疱疹有其特色,疗效显著。     

针灸治疗慢性功能性便秘现状分析

目的:探讨针灸治疗慢性功能性便秘(CFC)的规律.方法:对近10年针灸治疗CFC的中文文献资料进行整理分析.结果:现有的临床研究缺乏统一公认的适应症、取穴、操作方法;针灸可以治疗各个类型的CFC,穴位和操作方法需根据病变类型和病情来选择.结论:针灸治疗CFC安全、有效,需深化取穴规律、针刺规律等技术规范化的研究.     

穴位埋线治疗支气管哮喘疗法的特点及分析

目的:分析穴位埋线治疗支气管哮喘疗法的特点及作用。方法:运用文献计量学方法,以中国期刊全文数据库为资料来源,对穴位埋线治疗支气管哮喘进行定量分析。结果:检出有效文献93篇,涉及的期刊杂志达60种,完整的穴位埋线治疗支气管哮喘临床报道46篇,以临床研究类文献为主。结论:穴位埋线疗法治疗支气管哮喘有其特色,疗效显著。     

针灸结合其他疗法治疗腰椎间盘突出症随机临床对照研究文献的Meta分析

目的:通过文献研究,对针灸结合其他疗法治疗腰椎间盘突出症提供循证学依据。方法:应用Meta分析方法,对国内近5年来应用针灸结合其他疗法治疗腰椎间盘突出症并用单纯针灸治疗方法为对照的研究文献进行综合统计分析。结果:分别分析两组的临床有效率,经异质性检验,发现大部分研究对象同质,且以针灸结合其他治疗手法为主的治疗组疗效显著,差异有统计学意义(P0.05)。结论:现有的临床数据表明,应用针灸结合其他疗法治疗腰椎间盘突出症的疗效优于单纯针灸治疗。但由于纳入的RCT文献质量较低,特别是高质量、大样本、多中心的随机临床研究文献报道较少,本研究还有待进一步大规模的开展。     

穴位埋线治疗支气管哮喘文献计量学分析

目的:采用文献计量学方法对穴位埋线治疗支气管哮喘的国内中医(含中西医结合)现代文献特征进行分析,以总结规律,指导临床及科研。方法:检索中国知识网期刊全文数据库和重庆维普全文期刊数据库,获得国内有关穴位埋线治疗支气管哮喘的相关文献,对1990—2011年间国内医学期刊正式发表的56篇穴位埋线治疗哮喘文献进行科研属性、地区分布、研究方向、历年数量、穴位分布的计量学研究。结果:中医学术界在2005年以后开始重视穴位埋线治疗哮喘的研究,以病例报道为主,缺乏高质量的临床对照研究以及动物试验研究。研究的核心地区在于广东和河南,穴位选择上集中在肺俞、定喘、膻中3个穴位上,联合其他疗法的报道不多。结论:穴位埋线治疗哮喘的国内研究水平不断提高,已经形成热点研究地区及重点研究方向,但缺乏高质量的临床研究,创新性有待进一步加强。     

近5年针灸治疗银屑病的评价

本文对近5年针灸治疗银屑病的临床研究文献进行了综述，归纳了针灸对该病的各种疗法，包括针刺、剌络放血、埋线、穴位注射及综合治疗等。总结了银屑病的病因病机、针灸治疗的特点，提出存在问题及今后的研究方向。     

基于CNKI数据库的中医贴敷疗法文献计量学分析

目的定量分析中医贴敷疗法的研究现状和应用。方法检索CNKI数据库从建库到2020年1月的所有关于中医贴敷疗法的文献,按文献来源、发表年代、作者区域分布、文献类型、研究疾病、联合治疗等对文献进行筛选和分类,分析中医贴敷疗法的研究现状。结果 (1)共纳入中医贴敷疗法相关文献759篇,其中临床研究673篇、理论研究67篇、实验研究19篇,其中临床研究涉及了内科、外科、妇科、儿科、骨伤科、五官科等各个学科的疾病。(2) 1964年首次刊出关于中医贴敷疗法的文献,自2000年之后文献发表呈现快速增长的趋势;(3)中医贴敷疗法的发展呈现出区域不平衡性,30.17%的文献作者来自于华东地区,仅有5.47%的文献作者来自西南地区。(4) 763篇文献主要来源于期刊、学位论文、报纸、会议论文,其中579篇期刊分布在202种期刊上。(5)文献中涉及的联合治疗方法最常用是中药口服、针灸等中医联合治疗方法。结论 (1)中医贴敷疗法的研究已经具有一定的规模,临床应用范围不断扩大,已经广泛应用于内科、外科、妇科、儿科、骨伤科、五官科等,尤其在内科、儿科应用较多;(2)文献主要是临床研究,对于中医贴敷疗法的理论、机制等基础研究尚显不足;(3)文献质量不高,未来需要进行一些大样本、多中心的临床研究,为中医贴敷疗法的临床应用提供更高级别的循证医学证据,并为中医贴敷疗法的发展提供思路和方向。     

基于中医传承辅助平台对针灸治疗原发性痛经选穴组方规律分析

[目的]通过运用中医传承辅助平台,基于临床文献数据挖掘、分析针灸治疗原发性痛经的穴位配伍规律,总结针灸治疗原发性痛经的核心处方。[方法]检索1991~2017年中国知网期刊数据库收录的针灸治疗原发性痛经相关的临床研究文献,采用中医传承辅助平台分析、统计所有穴位中最常见穴位和穴位组合的频次以及核心穴位组合特点。[结果]共纳入204篇文献、287首穴位处方,得出针灸治疗原发性痛经多采用三阴交、关元、次髎、气海、地机、中极、足三里、肾俞、太冲、子宫等穴位及"中极、三阴交""气海、关元""气海、三阴交""关元、三阴交""关元,足三里"等常用穴位组合14个,并可得出针灸治疗原发性痛经"补益肝脾肾及冲任为主,兼以活血祛瘀行气祛湿"的用穴特点。[结论]运用中医传承辅助平台对研究数据结果进行整理分析,所得到的穴位配伍规律和核心处方可为临床治疗原发性痛经提供统计学依据及参考方案。     

基于数据挖掘探讨针灸治疗亨特综合征选穴规律

目的:采用数据挖掘探析现代临床文献中针灸治疗亨特综合征的选穴规律。方法:全面检索并筛选中国期刊全文数据库(CNKI)、万方期刊数据库(WF)、维普(VIP)等数据资源系统中的中文文献,选取从1994年1月至2019年3月符合以针灸治疗或其他中医外治法治疗亨特综合征的文献,对所选腧穴、穴位归经及其所属特定穴进行描述性分析,对相关数据进行二阶关联分析、三阶关联分析,并制作网络图。结果:经过严格筛选后,共纳入35篇文献,涉及相关腧穴51个,其中合谷穴应用频次最高;所选腧穴遍布十四经脉,其中足阳明胃经使用频次最多;对于交会穴的选择明显高于其他特定穴。结论:合谷为治疗亨特综合征的首选穴位,针刺治疗亨特综合征以阳明经脉为主,且重视局部取穴,配合辨证随证取穴,为亨特综合征的临床研究及治疗提供有效依据。     

近3年针灸治疗周围性面瘫的研究进展

目的整理近3年来针灸治疗周围性面瘫的临床报道和文献研究,对其临床研究进展进行总结。方法分别从针刺配合灸法、针刺配合刺络放血、针灸配合穴位埋线及针药结合等方面进行综述。结果针灸治疗周围性面瘫方法颇多,且疗效显著。结论针灸对于周围性面瘫的治疗具有独特的优势及良好的效果,在今后的临床中应进一步去研究其治疗机理,探讨针灸配合何种疗法疗效最佳,以提高临床疗效。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.