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1.
目的 优选鹿衔草中总鞣质最佳提取工艺.方法 采用正交试验,以提取率和总鞣质含量作为考察指标,对热回流、超声提取、冷浸提取等方法进行提取工艺考察.结果 以水为溶剂,提取方案采用热回流法,料液比为1∶8,温度为100 ℃,提取3次,每次1 h,提取率最高为7.63%.结论 热回流与超声提取、冷浸提取比较,所得提取率和总鞣质含量较高,该工艺操作简单,成本较低,水为溶剂环境友好,为进一步研究鹿衔草中总鞣质的提取方案提供依据.  相似文献   

2.
正交实验优选蒙药材芦巴子总鞣质的提取工艺研究   总被引:2,自引:2,他引:0  
目的:研究热回流提取芦巴子中鞣质的最佳因素。方法:以没食子酸为对照品,用紫外分光光度法测定热回流提取芦巴子中鞣质的提取率。结果与结论:采用150mL的70%乙醇作为提取溶剂,提取时间为1.5h所得样品中总鞣质含量最高,该方法可用于芦巴子中总鞣质的提取。  相似文献   

3.
目的:优选蒙药狗尾草果实中总鞣质的最佳提取工艺。方法:采用正交试验法L9(34)设计该试验,以狗尾草果实中的总鞣质含量作为考察指标,采用络合滴定法测定含量,考查影响鞣质提取的因素,优化狗尾草果实中总鞣质的提取方法和条件。结果:优选工艺为选用12倍药材量的70%丙酮,超声提取1 h,功率70 W。结论:优选工艺提取的总鞣质含量较高,该工艺合理可行。  相似文献   

4.
正交实验优选锁阳中总鞣质的提取工艺研究   总被引:1,自引:1,他引:1  
目的:优选中药锁阳的总鞣质最佳提取工艺。方法:采用正交实验L9(34)设计该实验,以总鞣质的含量作为考察指标,用比色法对锁阳总鞣质的提取工艺进行优选。结果:优选工艺为选用10倍药材量的70%丙酮,回流提取1h。结论:优选工艺提取的总鞣质的含量较高,该工艺合理可行。  相似文献   

5.
目的:筛选出舒筋止痛涂膜剂最佳提取工艺。方法:以乌头碱含量为指标,以渗漉法、冷浸法、回流法、水提法为考察因素,进行提取方法筛选,以浸泡时间、乙醇用量、乙醇浓度、液滴速度为考察因素,采用L9(34)正交试验筛选舒筋止痛涂膜剂最佳提取工艺条件。同时以肉桂挥发油含量为指标,进行肉桂挥发油最佳提取工艺研究。结果:川乌最佳提取方法为冷浸法,最佳提取工艺条件为加75%乙醇,12倍药材量醇,浸泡48 h以3.5 mL/min速度放液。肉桂挥发油最佳提取工艺为不浸泡,加8倍药材量水,回流提取6 h。结论:该提取工艺科学、合理、可行。  相似文献   

6.
7种山茱萸总皂苷提取分离方法效果比较   总被引:4,自引:0,他引:4  
目的:考察不同提取方法对山茱萸总皂苷含量测定的影响。方法:分别采用无水乙醇回流提取、无水乙醇回流-正丁醇萃取、体积分数为70%乙醇回流提取、体积分数为70%乙醇回流提取-正丁醇萃取、水提取、超声波提取和超临界CO2提取方法,以人参皂苷Re作对照品,比色法测定提取液中山茱萸总皂苷的含量。结果及结论:不同提取方法对皂苷含量有较大的影响,超临界CO2提取技术无溶剂残留,操作温度低,对环境友好,提取效率高,皂苷含量是传统溶剂提取的1.5~2.0倍,有较好的应用前景。  相似文献   

7.
不同提取方法对苦参生物总碱的影响   总被引:8,自引:0,他引:8  
目的:优选苦参中提取苦参生物总碱的提取方法.方法:采用酸性染料比色法 ,对苦参水回流、乙醇回流、乙醇渗滤三种提取液中的生物总碱进行含量测定.结果:水回流、乙醇回流、乙醇渗滤提取液中苦参总碱含量分别为3.03%、3.88%、 3.76%. 结论:以苦参总碱作为评价指标,乙醇回流结果较好,乙醇渗滤次之,水回流最低.  相似文献   

8.
目的建立地锦草总黄酮含量的测定方法及优化地锦草中总黄酮的提取工艺。方法采用紫外-可见分光光度法测定总黄酮含量;提取工艺考察了水回流提取、乙醇回流提取及超声提取不同的提取方法,优选出乙醇回流提取法,采用单因素试验及正交试验优化地锦草的乙醇回流提取工艺。结果该测定方法的平均回收率为102.82%,RSD=1.2%;地锦草总黄酮提取工艺考察,最终确定最佳工艺:75%乙醇,15倍量,提取2次,每次2 h;提取率高达到2.588%。结论优选的总黄酮提取工艺方法简单、易行。  相似文献   

9.
目的:优化中药复方降脂颗粒的提取工艺条件。方法:采用正交试验探讨乙醇浓度、加醇量、回流次数、提取时间等因素对中药复方降脂颗粒提取工艺中药物有效成分总黄酮的影响。结果:优选出乙醇回流提取最佳工艺条件为用20倍量70%乙醇,回流提取2次,每次1.5 h。平均得膏率为12.5%,总黄酮含量为4.49%。结论:经工艺验证证明该工艺稳定可行。  相似文献   

10.
正交法优化虎杖中白藜芦醇苷提取工艺的研究   总被引:3,自引:0,他引:3  
目的考察虎杖白藜芦醇苷的最佳提取分离工艺。方法采用正交实验优选提取分离条件,HPLC测定含量,丙酮重结晶纯化。结果醇提工艺的最优条件为:70%乙醇12倍量,回流提取3次,2h/次。结论该实验确定的最佳提取工艺稳定性好且简便易行。  相似文献   

11.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

12.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

13.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

14.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.  相似文献   

15.
Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P<0.01);the MAP decreased(P<0.01)and the HR increased drastically(P<0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.  相似文献   

16.
Objective:To investigate the gene expression of osteoprotegerin(OPG) and osteoclast differentiation factor(ODF) in the bone tissue of patients with hip fracture due to osteoporosis. Methods:OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers(over 50 years old) with hip fracture(Observer Group) and 30 cases of hip facture sufferers with no osteoporosis(Control group) were analyzed with the Semi-Quantitative RT-PCR method. Results:The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion:Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

19.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

20.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

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