动物组织中羟氨酸测定方法的建立及初步应用

目的：建立动物组织中的羟脯氨酸测定方法，以之估计胶原含量并衡量组织愈合程度。方法：研究显色温度、氧化时间，样品中盐浓度以及对－二甲氨基苯甲醛（ＰＤＡＢ）浓度对羟脯氨酸测定的影响。据此建立动物组织中的羟脯氨酸测定方法，并初步应用于实验动物犬贲门胶原含量的测定。     

外源性透明质酸在愈合中伤口的生化分析

目的探讨外源性透明质酸（ＨＡ）抑制瘢痕形成的作用及其可能机制。方法健康ＳＤ大鼠４５只，复制背部创伤模型，随机分成２％ＨＡ治疗组（Ａ组）、１％ＨＡ治疗组（Ｂ组）、ＰＢＳ对照组（Ｃ组），观察创面大体形态、平均愈合时间和残留面积的大小，测定愈合部位组织蛋白、羟脯氨酸（Ｈｙｐ）、Ⅲ型前胶原（ＰＣⅢ）的含量。结果⑴３组的平均愈合时间为（１５．２±２．０）ｄ、（１４．２±１．６）ｄ和（１３．４±１．４）ｄ，Ａ、Ｂ两组与Ｃ组比较有显著性差异，３组间比较也有显著性差异（Ｐ＜０．０５）。Ａ、Ｂ两组的残留面积与Ｃ组比较有显著性差异，３组间比较也有显著性差异（Ｐ＜０．０５）⑵Ｈｙｐ和ＰＣⅢ含量的测定：Ａ、Ｂ两组Ｈｙｐ含量的均值低于Ｃ组（Ｐ＜０．０１）；而ＰＣⅢ的含量却高于Ｃ组（Ｐ＜０．０１）。结论⑴ＨＡ通过对细胞的调控作用，有效地抑制胶原的合成，提高Ⅲ型胶原含量，使创伤愈合过程中瘢痕形成减少。⑵ＨＡ在创面愈合中抑制瘢痕形成的生物学作用与其浓度成正比关系。     

外源性透明质酸在愈合口伤口的生化分析

目的 探讨外源性透明质酸（ＨＡ）抑制瘢痕形成的作用及其可能机制。方法 健康ＳＤ大鼠４５只，复制背部创伤模型，随机分成２％ＨＡ治疗组（Ａ组）、１％ＨＡ治疗组（Ｂ组）、ＰＢＳ对照组（Ｃ组），观察创面大体形态、平均愈合时间和残留面积的大小，测定愈合部位组织蛋白、羟脯氨酸（Ｈｙｐ）、Ⅲ型前胶原（ＰＣⅢ）的含量。结果 （１）３组的平均愈合时间为（１５．２±２．０）ｄ、（１４．２±１．６）ｄ和（１３．４±１．     

枸杞多糖对密闭缺氧条件下小鼠心,肝,血SOD活性,MDA含量的影响

目的：探索枸杞多糖（ＬＢＰ）是否有抗脂质过氧化作用。方法：建立密闭缺氧的急性缺氧模式,采用ＮＢＴ测定ＳＯＤ活性、ＴＢＡ测定ＭＤＡ含量。结果：实验组与对照组织ＭＤＡ含量有显著性差别,其余组织ＳＯＤ、ＭＤＡ虽无显著性差异,但实验组均有ＳＯＤ活性升高,ＭＤＡ含是下降的趋势。结论：ＬＢＰ具有抗脂质过氧化作用的倾向。     

血小板源伤口愈合因子促糖尿病大鼠难愈伤口愈合的机理探讨

本实验以糖尿病大鼠海绵死腔伤为难愈伤口模型结合离体细胞培养,研究血小板源伤口愈合因子（ＰＤＷＨＦ）改善难愈伤口愈合的作用并探讨其可能机理。结果表明,局部应用ＰＤＷＨＦ后,伤口组织总ＤＮＡ〈羟脯氨酸含量增高,组织学表现为肉芽组织 成纤维细胞显著增生,胶原纤维沉积增加,离体实验发现ＰＤＷＨＦ可促进成纤维细胞的分化和增殖,提示,ＰＤＷＨＦ可通过促进伤口肉芽组织中的细胞合成代谢及增殖,增加胶原的合成与沉积     

兔角膜碱烧伤后角膜组织中G-6-PD和胶原酶活性变化

目的：探讨角膜组织中６ － 磷酸葡萄糖脱氢酶（Ｇ－ ６ － ＰＤ） 和胶原酶活性变化与角膜碱烧伤的关系。方法：用２ｍｏｌ／ＬＮａＯＨ溶液造成右眼角膜碱烧伤模型,以左眼为正常对照。分别于烧伤后不同时期测定两组角膜中的Ｇ－ ６ － ＰＤ 的活性和氧化型谷胱甘肽（ＧＳＳＧ） 及羟脯氨酸的含量。结果：与对照组相比,在烧伤后的１ － １６ 天,实验组的Ｇ－ ６ － ＰＤ活性和羟脯氨酸含量下降,而ＧＳＳＧ 含量升高,尤其是第８ 天的差异非常显著（Ｐ＜ ０ ．０１） ,至第３２ 天时上述指标基本接近对照组水平。Ｇ－ ６ － ＰＤ 活性与羟脯氨酸含量呈正相关,而与ＧＳＳＧ 含量呈负相关。结论：Ｇ－ ６ － ＰＤ活性降低导致ＧＳＳＧ 含量升高继而胶原酶活性增强,可能是角膜碱烧伤的机制之一。     

细胞因子TGF－β_1、PDGF－BB、bFGF在人肝纤维化中的作用

研究和探讨转化生长因子（ＴＧＦ）－β１、血小板源性生长因子（ＰＤＧＦ）－ＢＢ、碱性成纤维细胞生长因子（ｂＦＧＦ）在人肝纤维化中的作用，进一步阐明人体慢性肝病纤维化发生机制。外科切除的肝细胞标本按病变活动程度分为活动性、非活动性或轻度活动性以及对照组，以Ｎｏｒｔｈｅｒｎ分子杂交法测肝组织ＴＧＦ－β１ｍＲＮＡ，以免疫组化法显示组织原位ＰＤＧＦ－ＢＢ，ｂＦＧＦ及其相关细胞，并作半定量分析。结果：（１）肝病组织内ＴＧＦ－β１ｍＲＮＡ含量随肝病活动程度而增高（Ｐ＜０．０１）；（２）组织原位ＰＤＧＦ－ＢＢ、ｂＦＧＦ多肽定位及阳性细胞半定量显示，活动性肝病时两者均增高，阳性细胞所在部位与单核巨噬细胞以及胶原生成细胞一致，与Ⅰ、Ⅲ型胶原沉积也有密切关系。结论：本实验提示细胞因子ＴＧＦ－β１、ＰＤＧＦ－ＢＢ、ｂＦＧＦ在人肝纤维化中起着关键的调节作用。     

急性重复缺氧动物脑组织中单胺类神经递质含量的对比研究

以昆明小鼠为实验对象，用萤光法检测急性重复缺氧动物脑组织中ＤＡ、ＮＥ、５－ＨＴ，５－ＨＩＡＡ等４种单胺类物质的含量。结果发现：与未经缺氧处理的对照组（Ａ）、缺氧１次的实验对照组（Ｂ）及急性重复缺氧４次后饲养２ｄ实验对照组（Ｄ）等３组动物相比，急性重复缺氧４次实验组（Ｃ）动物脑组织中ＤＡ、５－ＨＩＡＡ含量显著升高，ＮＥ含量显著降低；５－ＨＴ较缺氧１次组显著上升，而Ａ、Ｂ、Ｄ３组动物脑组织中上述４种成分含量接近。结果提示脑组织中单胺类物质可能参与急性重复缺氧动物对缺氧耐受的形成。     

PDGF-AB对增生性瘢痕成纤维细胞增殖的影响

目的：探讨ＰＤＧＦ－ＡＢ促进伤口愈合的作用机理及其在瘢痕增生中的作用。方法：取体外培养的人正常皮肤及增生性瘢痕成纤维细胞（ＨＴＳＦＢ）测定生长曲线,用ＭＴＴ法观察ＰＤＧＦ－ＡＢ对其增殖影响的差异。结果：两种细胞生长曲线存在差异,ＰＤＧＦ－ＡＢ对两种成纤维细胞增殖均有明显刺激作用,且均呈剂量依赖性关系,但作用有差异。结论：ＰＤＧＦ－ＡＢ可能通过刺激成纤维细胞增殖促进伤口愈合,同时在瘢痕增生性疾病中起     

双能X线吸收法动态观察去势雌性大鼠不同部位骨量变化

有关骨质疏松（Ｏｓｔｅｏｐｏｒｏｓｉｓ,ＯＰ）发病机理和治疗的实验研究常需测定骨密度（Ｂｏｎｅｍｉｎｅｒａｌｄｅｎｓｉｔｙ,ＢＭＤ）。目前测定动物ＢＭＤ的方法包括双光子吸收法（ＤＰＡ）、单光子吸收法（ＳＰＡ）、定量计算机断层扫描（ＱＣＴ）［１］。这些...     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.