胃溃灵对胃粘膜损伤大鼠胃粘膜分泌的影响

目的 :研究胃溃灵对乙酸引起胃粘膜损伤大鼠胃粘膜分泌的影响。方法 :将 5 0只 SD大鼠制成乙酸胃粘膜损伤模型 ,并随机将大鼠等分为 5组 ,次日起给每组大鼠分别灌服等量生理盐水、大、中、小剂量 ( 12 g/ kg、6g/kg、3 g/ kg)胃溃灵、雷尼替丁 ,10 d后处死大鼠 ,观察大鼠胃粘膜损伤程度。采用阿尔新蓝与胃液中糖蛋白结合的方法 ,分别测定大鼠胃内游离粘液量、胃壁粘液量。结果 :胃溃灵能明显提高大鼠胃内游离粘液、胃壁粘液的分泌量 ,能明显抑制乙酸对大鼠胃粘膜的损伤。结论 :胃粘液分泌量增加可加强对粘膜的屏障作用 ,这是胃溃灵保护胃粘膜损伤的机制之一。     

胃痛娄对大鼠胃粘膜血流量及脾虚大鼠模型的影响

为深入探讨胃痛灵保护胃粘膜的作用机制，研究了胃痛灵（ＷＴＬ）对无水乙醇损伤大鼠胃粘膜血流量（ＧＭＢＦ）、脾虚大鼠Ｄ－木糖吸收率、胃壁结合粘液量以及胃酸分泌和胃蛋白酶活性的影响。结果显示ＷＴＬ能够明显增中大鼠ＧＭＢＦ、胃壁结合粘液量，提高脾虚大鼠Ｄ－木糖吸收率，增强胃粘膜防御机能，但对脾虚大鼠胃酸的分泌和胃蛋白酶均无明显影响，在体外也无中和胃酸的能力。     

胃痛灵对大鼠胃粘膜血流量及脾虚大鼠模型的影响

为深入探讨胃癌灵保护胃粘膜的作用机制，研究了胃痛灵（WTL）对无水乙醇损伤大鼠胃粘膜血流量（GMBF）、脾虚大鼠D-木糖吸收率、胃壁结合粘液量以及胃酸分泌和胃蛋白酶活性的影响。结果显示WTL能够明显增加大鼠GMBF（P＜0．05）、胃壁结合粘液量（P＜0．05），提高脾虚大鼠D-木糖吸收率（P＜0．05），增强胃粘膜防御机能，但对脾虚大鼠胃酸的分泌和胃蛋白酶活性均无明显影响。在体外也无中和胃酸的能力。     

乳香提取物对大鼠乙酸胃溃疡愈合质量的影响

目的:研究乳香提取物对大鼠乙酸胃溃疡愈合质量的影响.方法:用冰醋酸制备大鼠慢性胃溃疡模型,随机分为6组,分别灌服0.85%氯化钠溶液、乳香提取物、雷尼替丁等.用苏木精-伊红染色、粘液组织化学染色和苦味酸-酸性品红染色对大鼠愈合性胃溃疡再生粘膜厚度、再生粘膜粘液含量、囊状扩张腺体数量、炎症细胞浸润数量等进行定量观察.结果:乳香提取物组再生粘膜厚度增加、囊状扩张腺体数量减少和粘液高碘酸无色品红(PAS)含量增加;肉芽组织胶原含量增加,炎症细胞浸润数量减少.结论:乳香提取物能提高溃疡再生粘膜结构和功能成熟度,提高溃疡愈合质量.     

柴胡桂枝汤对大鼠乙酸胃溃疡愈合质量的影响

目的研究柴胡桂枝汤对大鼠乙酸胃溃疡愈合质量的影响。方法用冰醋酸制备大鼠慢性胃溃疡模型,随机分为A、B、C三组,分别灌服柴胡桂枝汤、西咪替丁、生理盐水。用苏木精-伊红染色和粘液组织化学染色对大鼠愈合性胃溃疡再生粘膜进行定量观察。用硝酸还原酶法检测胃粘膜NO含量。结果柴胡桂枝汤组和西咪替丁组再生粘膜厚度、粘液指数高于生理盐水组(P＜0.01),粘膜肌层缺损宽度小于生理盐水组(P＜0.01),NO含量高于生理盐水组(P＜0.01～0.05);柴胡桂枝汤组粘液指数及NO含量高于生理盐水组(P＜0.01～0.05)。结论柴胡桂枝汤能提高溃疡愈合质量,是临床抗消化性溃疡复发的可能机制之一。     

柴胡桂枝汤对大鼠乙酸胃溃疡愈合质量的影响

目的：研究柴胡桂枝汤对大鼠乙酸胃溃疡愈合质量的影响。方法：用冰醋酸制备大鼠慢性胃溃疡模型，随机分为Ａ、Ｂ、Ｃ三组，分别灌服柴胡桂枝汤、西咪替丁、生理盐水。用苏木精－伊红染色和粘液组织化学染色对大队愈合性胃溃疡再生粘膜进行定量观察。用硝酸还原酶法检测胃粘膜ＮＯ含量。结果：柴胡桂枝汤组和西咪替丁组再生粘膜厚度、粘液指数高于生理盐水组（Ｐ〈０．０１），粘膜肌层缺损宽度小于生理盐水组（Ｐ〈０．０１），ＮＯ     

促愈颗粒对胃溃疡实验大鼠胃黏膜表皮生长因子及其受体表达的影响

[目的]研究促愈颗粒对乙酸烧灼型胃溃疡(GU)大鼠胃黏膜表皮生长因子(EGF)及其受体(EGFR)表达的影响。[方法]将大鼠随机分为4组:正常对照组,模型组,促愈颗粒组和雷尼替丁组。乙酸制备慢性GU大鼠模型后,于给药14 d和28 d后分2次处死大鼠,观察胃黏膜组织形态,免疫组织化学技术检测大鼠胃黏膜EGF及EGFR水平。[结果]与模型组比较,促愈颗粒组和雷尼替丁组囊状扩张腺体数量均显著减少(P<0.01,<0.05),EGF及EGFR水平均显著增高(P<0.01,<0.05),且促愈颗粒组作用均优于雷尼替丁组(均P<0.05)。[结论]促愈颗粒可能通过增加胃黏膜EGF和EGFR的水平,进而提高GU再生黏膜结构和功能成熟度,从而促进溃疡愈合,提高溃疡愈合质量,并防止溃疡复发。     

复方中药安胃汤提高大鼠胃溃疡愈合质量的机制

目的:通过实验性乙酸大鼠胃溃疡模型,研究复方中药安胃汤提高慢性胃溃疡愈合质量机制．方法:40只Wistar大鼠随机平均分为正常对照组、模型组、安胃汤组和雷尼替丁．采用乙酸浸渍法建立胃溃疡模型,造模3 d后前两组分别用生理盐水灌胃,后两组分别用安胃汤和雷尼替丁灌胃．采用放射免疫方法和免疫组织化学方法观察复方中药安胃汤对大鼠胃溃疡模型愈合时血清表皮生长因子(EGF)和胃黏膜 EGF,表皮生长因子受体(EGFR)及转化生长因子-β1(TGF-β1)表达的影响．结果:模型组相比,安胃汤组和雷尼替丁组可提高血清EGF(1．12±0．24,0．99±0．15μg／L vs0．52±0．13μg／L,P<0．01),安胃汤组与雷尼替丁组相比差异也有显著意义(P<0．05)．与模型组相比,安胃汤组和雷尼替丁组可显著增强胃黏膜EGF、EGFR及TGF-β1表达(EGF: 29．7％±1．9％,26．5％±1．6％vs18．4％±2．0％, P<0．01:EGFR:29．6％±2．6％,25．9％±1．0％vs 20．4％±1．8％,P<0．01;TGF-β1:67．0％±2．0％, 49．5％±1．1％vs27．3％±1．0％,P<0．01),安胃汤组强于雷尼替丁组(均P<0．01)．结论:安胃汤可能通过提高血清EGF和增强胃黏膜EGF,EGFR及TGF-β1表达,增强胃黏膜保护作用而提高溃疡愈合质量．     

促愈颗粒对大鼠乙酸胃溃疡愈合质量的影响

目的:观察促愈颗粒对大鼠乙酸胃溃疡愈合质量的影响.方法:用冰醋酸制备大鼠慢性胃溃疡模型,所有大鼠随机分为4组(空白模型组、促愈颗粒组、雷尼替丁组和正常对照组).HE染色观察大鼠愈合性胃溃疡再生黏膜腺体成熟度和炎症细胞浸润情况;硝酸还原法检测血清NO含量;放射免疫法检测血浆PGE2含量;免疫组织化学技术检测大鼠胃黏膜EGF的表达情况;透射电镜观察大鼠再生黏膜超微结构的变化.结果:给药28d后与模型组和雷尼替丁组比较,促愈颗粒组再生黏膜囊性扩张腺体数量及炎细胞数量明显减少(再生黏膜囊性扩张腺体数量:1.43±0.53 vs 3.84±1.08.2.36±0.79,P<0.01和P<0.05;炎细胞数量:9.92±2.66 vs 28.32±6.96,17.92±4.76,P<0.01和P<0.05);血清NO及血浆PGE2含量显著增高(NO:105.41±8.02μmol/g vs 67.35±16.85,79.1 8±28.05μmol/g,P<0.0 1或P<0.05;PGE2:125.50±11.95 ng/L vs 97.33±11.84,118.83±13.25 ng/L,P<0.01和P>0.05);胃黏膜EGF表达明显增强(25.38±5.17 vs 16.82±2.13,21.12±6.08,P<0.05和P<0.05);促愈颗粒组超微结构的恢复亦优于模型组和雷尼替丁组.结论:促愈颗粒能提高溃疡再生黏膜结构,功能成熟度及电镜下成熟度,从而提高溃疡愈合质量.     

胃康胶囊对醋酸烧灼型胃溃疡大鼠血液细胞因子含量的影响

目的：观察胃康胶囊对醋酸烧灼型胃溃疡大鼠血液中相关细胞因子[前列腺素I2（PGI2）、血小板活化因子（PAF）、肿瘤坏死因子α（TNF－α）、表皮生长因子（EGF）含量的影响,探讨其在溃疡愈合过程中的分子机制。方法：Wistar大鼠30只,用冰醋酸复制成慢性胃溃疡模型,灌胃给药2周后处死大鼠进行血液检测。结果：胃康胶囊能降低胃溃疡大鼠血液中细胞因子PAF、TNF－α的含量,升高PGI2、EGF的含量。对抗胃酸分泌增加。结论：胃康胶囊能有效地促进胃溃疡病变的愈合,对胃溃疡有治疗作用。     

愈疡止痛煎剂对十二指肠溃疡大鼠溃疡愈合质量的影响

[目的]探讨愈疡止痛(YYZT)煎剂对实验性大鼠十二指肠溃疡(DU)愈合质量的影响.[方法]用L-半胱胺酸盐酸盐复制大鼠DU模型,设立空白对照(A)组、病理模型(B)组、YYZT(C)组、雷尼替丁(D)组.3周后处死动物,观察溃疡愈合程度并评价溃疡愈合质量,测定胃液pH值及血清表皮生长因子(EGF)水平.[结果]C组胃酸较其余各组均降低(P＜0.05);C组胃液pH值及血清EGF较其余各组明显升高(P＜0.05,＜0.01).[结论]YYZT煎剂在提高溃疡愈合方面有明显优势,其作用机制可能与减少胃酸分泌、升高血清EGF有关.     

七方胃痛胶囊对大鼠乙酸胃溃疡愈合质量的影响

[目的]观察七方胃痛胶囊对大鼠实验性胃溃疡愈合质量(QOUH)的影响.[方法]予冰醋酸注射制作大鼠胃溃疡模型后,随机分为5组:分别予0.85%氯化钠液、雷尼替丁胶囊、七方胃痛胶囊(小、中、大3个剂量),连续灌胃14 d,处死大鼠,制作病理切片,予苏木精-伊红染色和黏液组织化学染色,在光镜下观察溃疡指数、再生黏膜厚度、黏膜肌层缺损宽度、表面黏液厚度、再生组织结构.[结果]七方胃痛胶囊能增加再生黏膜厚度及表面黏液厚度,减少再生黏膜肌层缺损宽度(P＜0.01),有量效关系(P＜0.01),能明显改善再生组织结构.[结论]七方胃痛胶囊能改善再生组织结构,从而提高QOUH,达到抗溃疡复发的作用.     

健胃汤抗大鼠乙酸胃溃疡及泼尼松再损伤的实验研究

目的 :从溃疡愈合质量 (QOUH )角度探讨健胃汤抗消化性溃疡 (PU )复发的可能机制。方法 :采用大鼠乙酸胃溃疡模型 ,观察健胃汤对该模型及泼尼松诱导的再损伤胃粘膜的影响 ,并测定其一氧化氮 (NO)、表皮生长因子 (EGF)的含量。结果 :健胃汤能改善胃粘膜的组织损伤并提高溃疡周围组织的 NO、EGF含量。结论 :健胃汤能提高 QOUH ,增强溃疡瘢痕处抗泼尼松再损伤能力 ;提高溃疡周围组织的 NO、EGF含量 ,这可能是其抗 PU复发的机制之一。     

粘膜功能恢复与消化性溃疡愈合的关系

粘膜形态学、组织学以及功能学的恢复有助于提高消化性溃疡的愈合质量,减少溃疡复发。文章就溃疡粘膜的成熟度即粘膜功能的恢复与消化性溃疡愈合质量之间的关系作一综述。     

四逆泻心汤对慢性乙酸胃溃疡大鼠溃疡愈合的影响

[目的]观察四逆泻心汤（SNXX）对慢性乙酸胃溃疡大鼠溃疡愈合的影响。[方法]SD大鼠40只，随机取10只作空白对照（A）组，行假手术处理，剩余大鼠用乙酸腐蚀法制慢性胃溃疡模型，造模后存活大鼠随机分为模型对照（B）组，SNXX治疗（C）组，雷尼替丁治疗（D）组。治疗3周后，测量溃疡体积、面积；光镜下评价溃疡愈合质量（QOUH），测定血清一氧化氮（NO）、表皮生长因子（EGF）水平。[结果]C组溃疡体积缩小优于B组（P〈0．01）、D组（P〈0.05）；D组溃疡面积缩小优于B组（P〈0．01），C组溃疡面积较B组有缩小趋势，但无统计学意义。C组光镜下QOUH优于B、D组；血清NO水平低于B组（P〈0．01）、D组（P〈0．05）；血清EGF水平高于A组（P〈0．01）、B组（P〈0．05），较D组有升高趋势，但无统计学意义。[结论]SNXX可促进大鼠慢性乙酸胃溃疡愈合，溃疡体积缩小及QOUH明显优于雷尼替丁，溃疡面积缩小与雷尼替丁的差异无统计学意义，降低NO水平，升高EGF水平可能为其机制之一。     

Quality of healing of gastric ulcers: Natural products beyond acid suppression

Gastric ulcer is a chronic disease featured with unexpected complications, including bleeding, stenosis and perforation, as well as a high incidence of recurrence. Clinical treatments for gastric ulcer have allowed the rapid development of potent anti-ulcer drugs during the last several decades. Gastric ulcer healing is successful with conventional treatments including H2-receptor antagonists, and proton pump inhibitors(PPIs) have been essential for ulcer healing and prevention of complications. Additionally, Helicobacter pylori eradication therapy is effective in reducing ulcer recurrence and leads to physiological changes in the gastric mucosa which affect the ulcer healing process. However, in spite of these advancements, some patients have suf-fered from recurrence or intractability in spite of continuous anti-ulcer therapy. A new concept of the quality of ulcer healing(QOUH) was initiated that considers the reconstruction of the mucosal structure and its function for preventing ulcer recurrence. Although several gastroprotection provided these achievements of the QOUH, which PPI or other acid suppressants did not accomplish, we found that gastroprotection that originated from natural products, such as a newer formulation from either Artemisia or S-allyl cysteine from garlic, were very effective in the QOUH, as well as improving clinical symptoms with fewer side effects. In this review, we will introduce the importance of the QOUH in ulcer healing and the achievements from natural products.     

根除幽门螺杆菌对胃溃疡愈合质量及复发的影响研究

目的探讨幽门螺杆菌（Helicobacter pylori，Up）对胃溃疡愈合质量及复发的影响。方法120例坳阳性活动期胃溃疡患者，口服泮托拉唑、阿莫西林、甲硝唑一周后，继续口服泮托美拉唑5周。治疗结束后复查胃镜取病理组织学检查并检测Hp根除情况，对比却根除组与却根除失败组内镜下溃疡愈合形态差异和愈合质量（包括内镜下再生黏膜成熟度和再生黏膜组织学成熟度）。所有患者随诊1年以上了解溃疡复发情况。结果治疗后92例胃溃疡患者月p检测阴性，坳根除率为80．43％；却根除组与跏根除失败组在内镜下愈合率方面的差异无显著性意义（P〉0．05），但两组在再生黏膜成熟度和再生黏膜组织学成熟度方面的差异有显著性意义（P〈0．01）。坳根除组1年溃疡复发率为4．35％，却根除失败组为21．43％，两组差异有显著性意义（P〈0．05）。结论根除Hp可提高胃溃疡的愈合质量，减少溃疡病复发。     

Influence of gender difference and gastritis on gastric ulcer formation in rats

BACKGROUND: Male patients with gastritis are found to have a high risk of developing peptic ulcer diseases. However, how gastritis or gender difference affects gastric ulcer formation is unclear. The present study aimed to investigate the relationship between ethanol-induced acute gastritis and gastric ulcer formation in rats. METHODS: Acute gastritis or gastric ulcer was induced in the rat stomach by 80% ethanol or 60% acetic acid, respectively. Rats were killed either with gastritis alone or thereafter at day 1, 3 or 6 after ulcer induction. The number of proliferating and apoptotic cells, the mucosal mucus and prostaglandin E(2) (PGE(2)) level were also determined. RESULTS: Male rats with acute gastritis potentiated gastric ulcer formation, while gastritis in female rats prevented ulceration. Female rats with gastritis had a significantly faster ulcer-healing rate. More apoptotic cells were found in the gastritis groups, but only the female gastritis group produced more proliferating cells and had a decrease in the apoptosis-over-proliferation ratio. The mucus level was higher in female rats after ulcer induction. Mucosal PGE(2) level was higher in female rats with acute gastritis. Both mucus and PGE(2) were increased during ulcer healing in both genders. CONCLUSIONS: This study shows that gender difference plays a role in the pathogenesis of ulcer formation. The number of cells with apoptosis or proliferation determines, in part, the gender difference on gastric ulcer formation in rats. Gastric PGE(2) not only contributes to this process, but also together with gastric mucus participates in the ulcer-healing process in the stomach.     

Quality of healing of gastric ulcers: Natural products beyond acid suppression简

Gastric ulcer is a chronic disease featured with unexpected complications, including bleeding, stenosis and perforation, as well as a high incidence of recurrence. Clinical treatments for gastric ulcer have allowed the rapid development of potent anti-ulcer drugs during the last several decades. Gastric ulcer healing is successful with conventional treatments including H₂-receptor antagonists, and proton pump inhibitors (PPIs) have been essential for ulcer healing and prevention of complications. Additionally, Helicobacter pylori eradication therapy is effective in reducing ulcer recurrence and leads to physiological changes in the gastric mucosa which affect the ulcer healing process. However, in spite of these advancements, some patients have suffered from recurrence or intractability in spite of continuous anti-ulcer therapy. A new concept of the quality of ulcer healing (QOUH) was initiated that considers the reconstruction of the mucosal structure and its function for preventing ulcer recurrence. Although several gastroprotection provided these achievements of the QOUH, which PPI or other acid suppressants did not accomplish, we found that gastroprotection that originated from natural products, such as a newer formulation from either Artemisia or S-allyl cysteine from garlic, were very effective in the QOUH, as well as improving clinical symptoms with fewer side effects. In this review, we will introduce the importance of the QOUH in ulcer healing and the achievements from natural products.