邻苯二甲酸正丁酯净化自体骨髓移植治疗白血病

邻苯二甲酸正丁酯（ＤＢＰ）在体外具有选择性杀伤白血病细胞的特性，白血病骨髓体外培养体系中加入ＤＢＰ会加速白血病祖细胞的清除。在骨髓体外长期培养基础上，利用ＤＢＰ对１４例急性非淋巴细胞白血病病人骨髓进行体外净化并自体骨髓移植，骨髓细胞经过５～９ｄ与５０μｇ／ｍｌＤＢＰ共培养后，骨髓有核细胞回收率为６８％，ＣＦＵ－ＧＭ回收率为７０．１％，回输骨髓细胞悬液中检测不出残留ＤＢＰ。净化后骨髓回输给放，化疗预     

气相色谱分析邻苯二甲酸正丁酯与人血浆共孵育后的浓度变化

邻苯二甲酸正丁酯（ＤＢＰ）能选择性地抑制肿瘤细胞的生长，并已应用于体外净化人骨髓细胞中残留白血病细胞〔１，２〕。前文报道了ＤＢＰ与人骨髓细胞共孵育后的浓度变化〔３〕。本研究用气相色谱法分析ＤＢＰ与人血浆孵育前后的浓度变化，了解ＤＢＰ进入血液后的分解情...     

邻苯二甲酸正丁酯结合骨髓体外培养净化白血病细胞方法的建立①

邻苯二甲酸正丁酯结合骨髓体外培养净化白血病细胞方法的建立①１００８５０北京军事医学科学院放射医学研究所徐黎，王立生，裴雪涛，吴祖泽中国图书资料分类号７３３．７体外净化骨髓中的白血病细胞是降低自体骨髓移植后白血病复发率的重要措施。我们在实验研究中证明邻...     

大容量骨髓体外净化初步临床应用

应用补体介导细胞毒法体外净化骨髓中残留白血病细胞后，对普通型急性淋巴细胞白血病（CALL）患者4例进行了自身骨髓移植（ABMT）。单个核细胞经单克隆抗体55（McAb55)加兔补体两次处理后，CALL抗原阳性细胞清除率可达3个对数级，骨髓有核细胞及CFU－GM回收率分别为25．8％－64．8％和40．0％－62．4％，外周血细胞于移植后第11－13天开始回升，15－29天升至10^9／L以上。第23－53天出洁净室，现无病存活时间分别为8^ 、7^ 、3^ 和1．5个月，远期疗效仍在定期随访中。     

大容量骨髓体外净化及初步临床应用

应用补体介导细胞毒法体外净化骨髓中残留白血病细胞后,对普通型急性淋巴细胞白血病(CALL)患者4例进行了自身骨髓移植(ABMT)。单个核细胞经单克隆抗体55(McAb 55)加兔补体两次处理后,CALL抗原阳性细胞清除率可达3个对数级,骨髓有核细胞及CFU-GM回收率分别为25.8％～64.8％和40.0％～62.4％,外周血白细胞于移植后第11～13天开始回升,15～29天升至10~9/L以上。第23～53天出洁净室,现无病存活时间分别为8~+、7~+,3~+和1.5个月,远期疗效仍在定期随访中。     

尿液CD14+细胞测定对血尿来源的鉴别诊断价值

为了解尿液ＣＤ１４＋细胞测定在血尿来源诊断中的意义，采用流式细胞术分别测定６０例肾小球性血尿和４０例非肾小球性血尿患者尿液ＣＤ１４＋细胞百分率（ＣＤ１４Ｐ）及部分病例的外周血ＣＤ１４Ｐ。结果表明，肾小球性血尿组尿液ＣＥ１４Ｐ〉５者占５４例（９０％），〈３者２例（３．３％）；非肾小球性血尿组ＣＤ１４Ｐ〉３为３例（７．５％），ＣＤ１４Ｐ〉５者仅１例（２．５％）；非肾小球性血尿组尿ＣＤ１４Ｐ与外周血ＣＤ     

低分子天然抑瘤物净化的自体骨髓移植治疗白血病一例报告

利用某些生物因子抑制肿瘤细胞生长或诱导其分化的特性是净化白血病骨髓的一条可行途径。吴祖泽等的研究发现,在人胎儿组织中存在着一类低分子量的天然肿瘤抑制物(LMW-NTS),它对人或小鼠的多种白血病细胞株以及髓系白血病病人原代白血病祖细胞(L-CFU)均有明显的、不可逆的抑制作用,而对正常造血细胞影响较轻,从而在体外液体培养条件下可用于净化骨髓中残留的白血病细胞。我们利用这一选择性抑瘤作用,对一例 AML 病人缓解期的骨髓作了体外净化后的自体骨髓移植,获得成功,现报告如下。     

骨髓的体外净化并自身移植5例报告

我们用单克隆抗体(McAb)和兔补体体外净化骨髓中残留白血病细胞后对5例普通型急性淋巴细胞性白血病(CALL)患者进行了自身骨髓移植(ABMT)。抽出的骨髓细胞经McAb55加兔补体两次处理后,CALL抗原(CALLA)阳性细胞清除率可达2～3个对数级,骨髓有核细胞及CFU-GM回收率分别为48.7％～96.9％和39.5％～95.7％。外周血白细胞于移植后11～16d开始回升,14～29d升至1×10~9/L以上。23～53d出洁净室。例3于移植后4个月白血病复发, 8.2个月死亡。另4例无病活存分别为19~+、18~+、12~+和6~+个月,远期疗效正在定期随访中。     

小鼠骨髓细胞及骨髓基质细胞混合输注对骨髓移植后造血重建的影响

观察小鼠骨髓细胞及骨髓基质细胞混合输注对骨髓移植后造血重建的影响。给予受致死剂量照射的Ｂａｌｂ／ｃ小鼠输注同基因骨髓细胞１×１０７ 个及经体外扩增的同基因原代骨髓基质细胞 ２×１０５ 个,与单纯骨髓移植组比较,移植后第１４ 天外周血白细胞、血小板回升较快,第１５ 天、第２０ 天ＢＦＵ－ＧＭ、ＢＦＵ－Ｅ、ＣＦＵ－Ｅ、ＣＦＵ－Ｓ明显高于后者,第２０ 天已达正常。　结论：原代骨髓基质细胞不仅是可以移植的,而且能够加快造血重建,提高移植效果。     

重组人γ─干扰素联合肿瘤坏死因子或高热体外净化实验研究

用重组人γ－干扰素（ＩＦＮ）和肿瘤坏死因子（ＴＮＦ）体外净化骨髓中残存的白血病细胞时，１０００Ｕ／ｍｌＩＦＮ或１００Ｕ／ｍｌＴＮＦ单独应用时仅能清除大约８０％左右，而二者联用时则能起到协同抗白血病的效应，可清除９０％以上残存的白血病细胞，但对ＣＦＵ－ＧＭ的形成却没有协同抑制作用，抑制率仅为５４％。在高热体外净化研究中，单独加热到４１℃作用２ｈ，清除率达９０％，若同时加入１０００Ｕ／ｍｌ的ＩＦＮ，可大大增强高热的净化效果，清除率可达９８％以上，且减轻了高热对ＣＦＵ－ＧＭ的抑制作用，ＣＦＵ－ＧＭ的抑制率下降为２４．５％，与单纯高热净化时的３２．８％相比，Ｐ＜０．０５。因此可以利用提高温度的办法来进一步达到体外净化的目的     

Knee injury and Osteoarthritis Outcome Score (KOOS) - validation of a Swedish version

The Knee injury and Osteoarthritis Outcome Score (KOOS) is a self-administered instrument measuring outcome after knee injury at impairment, disability, and handicap level in five subscales. Reliability, validity, and responsiveness of a Swedish version was assessed in 142 patients who underwent arthroscopy because of injury to the menisci, anterior cruciate ligament, or cartilage of the knee. The clinimetric properties were found to be good and comparable to the American version of the KOOS. Comparison to the Short Form-36 and the Lysholm knee scoring scale revealed expected correlations and construct validity. Item by item, symptoms and functional limitations were compared between diagnostic groups. High responsiveness was found three months after arthroscopic partial meniscectomy for all subscales but Activities of Daily Living.     

The acutely limping child

Acute limping may be the result of multiple pathologies in children. The differential diagnosis varies based on the age of the child. Irrespective of age, the initial imaging work-up includes AP and frog leg radiographs of the pelvis and ultrasound; MRI may sometimes be helpful. In children less than 3 years, infections and trauma are most frequent. MRI is the imaging modality of choice when osteomyelitis is clinically suspected. Between the ages of 3 and 10 years, transient synovitis of the hip and Legg-Calvé-Perthes disease are main considerations but infection, inflammation and focal bony lesions are also considered. In children over 10 years, slipped capital femoral epiphysis also is considered.     

Do Balance Board Training Programs Reduce the Risk of Ankle Sprains in Athletes?

Introduction Ankle sprains are the most common musculo-skeletal injury that occurs in athletes,particularly in sports that require jumping and landing on one foot such as soccer,and basketball(1-4).These injuries often result in significant time loss from participation,long-term disability,and have a major impact on health care costs and resources(5-8).     

High Intensity Interval Training:New Insights

KEY POINTS ·High-intensity interval training(HIT)is characterized by repeated sessions of relatively brief，intermittent exercise.often performed with an“a11 out”effort or at an intensity close to that which elicits peak oxygen uptake(i.e.，≥90%of VO2 peak).     

Endovascular management of carotid-cavernous fistulas

Objective To investigate endovascular treatment of traumatic direct carotid-cavernous fistulas （CCF） and their complications such as pseudoaneurysms. Methods： Over a five-year period, 22 patients with traumatic direct CCFs were treated endovascularly in our institution. Thirteen patients were treated once with the result of CCF occluded, 8 twice and 1 three times. Treatment modalities included balloon occlusion of the CCF, sacrifice of the ipsilateral internal carotid artery with detachable balloon, coll embolization of the cavernous sinus and secondary pseudoaneurysms, and covered-stem management of the pseudoaneurysms. Results All the direct CCFs were successfully managed endovascularly. Four patients developed a pseudoaneurysm after the occlusion of the CCF with an incidence of pseudoaneurysm formation of 18.2% （4/22）. A total number of 8 patients experienced permanent occlusion of the ICA with a rate of ICA occlusion reaching 36.4% （8/22）. Followed up through telephone consultation from 6 months to 5 years, all did well with no recurrence of CCF symptoms and signs. Conclusion Traumatic direct CCFs can be successfully managed with endovascular means. The pseudoaneurysms secondary to the occlusion of the CCFs can be occluded with stent-assisted coiling and implantation of covered stents.     

Developmental validation of the PowerPlex(®) ESI 16 and PowerPlex(®) ESI 17 Systems: STR multiplexes for the new European standard

In response to the ENFSI and EDNAP groups’ call for new STR multiplexes for Europe, Promega^® developed a suite of four new DNA profiling kits. This paper describes the developmental validation study performed on the PowerPlex^® ESI 16 (European Standard Investigator 16) and the PowerPlex^® ESI 17 Systems. The PowerPlex^® ESI 16 System combines the 11 loci compatible with the UK National DNA Database^®, contained within the AmpFlSTR^® SGM Plus^® PCR Amplification Kit, with five additional loci: D2S441, D10S1248, D22S1045, D1S1656 and D12S391. The multiplex was designed to reduce the amplicon size of the loci found in the AmpFlSTR^® SGM Plus^® kit. This design facilitates increased robustness and amplification success for the loci used in the national DNA databases created in many countries, when analyzing degraded DNA samples. The PowerPlex^® ESI 17 System amplifies the same loci as the PowerPlex^® ESI 16 System, but with the addition of a primer pair for the SE33 locus. Tests were designed to address the developmental validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM), and those of the DNA Advisory Board (DAB). Samples processed include DNA mixtures, PCR reactions spiked with inhibitors, a sensitivity series, and 306 United Kingdom donor samples to determine concordance with data generated with the AmpFlSTR^® SGM Plus^® kit. Allele frequencies from 242 white Caucasian samples collected in the United Kingdom are also presented. The PowerPlex^® ESI 16 and ESI 17 Systems are robust and sensitive tools, suitable for the analysis of forensic DNA samples. Full profiles were routinely observed with 62.5 pg of a fully heterozygous single source DNA template. This high level of sensitivity was found to impact on mixture analyses, where 54–86% of unique minor contributor alleles were routinely observed in a 1:19 mixture ratio. Improved sensitivity combined with the robustness afforded by smaller amplicons has substantially improved the quantity of data obtained from degraded samples, and the improved chemistry confers exceptional tolerance to high levels of laboratory prepared inhibitors.     

Prevalence and severity of hip and groin pain in sub‐elite male football: a cross‐sectional cohort study of 695 players

The purpose of this study was twofold: (a) to investigate the prevalence of hip and groin pain in sub‐elite male adult football in Denmark and (b) to explore the association between prevalence and duration of hip and groin pain in the previous season with the Copenhagen Hip and Groin Outcome Score (HAGOS) in the beginning of the new season. In total 695 respondents from 40 teams (Division 1–4) were included. Players completed in the beginning of the new season (July–Sept 2011) a self‐reported paper questionnaire on hip and/or groin pain during the previous season and HAGOS. In total 49% (95% CI: 45–52%) reported hip and/or groin pain during the previous season. Of these, 31% (95% CI: 26–36%) reported pain for >6 weeks. Players with the longest duration of pain during the previous season had the lowest HAGOS scores, when assessed at the beginning of the new season, P < 0.001. This study documents that half of sub‐elite male adult football players report pain in the hip and/or groin during a football season. The football players with the longest duration of pain in previous season displayed the lowest HAGOS scores in the beginning of the new season.     

Bronchial stump fistula :treatment with covered retrievable hinged metallic stents-preliminary clinical experience

Objective To evaluate the preliminaily clinical efficacy and retrievability of a retrievable hinged covered metallic stent in the treatment of the bronchial stump fistula （BSF）. Methods Between April 2003 and March 2005, 8 patients with bronchial stump fistula after pneumonectomy or lobectomy were treated with two types （A and B） of retrievable hinged covered metallic stents. Type A stent was placed in 6 patients and type B in 2 under fluoroscopic guidance. The stent was removed with a retrieval set when BSF was healed or complications occurred. Results Stent placement in the bronchial tree was technically successful in all patients, without procedure-related complications. Immediate closure of the BSF was achieved in all patients after the procedure. Stents were removed from all patients but one. Removal of the stents was difficult in two patients due to tissue hyperplasia. Patients were followed up for 6 - 21 months. Placement of the stents remained stable in all patients except one due to severe cough. Permanent closure of BSF was achieved in 7 （87.5%） of 8 patients. Conclusion Use of a retrievable hinged covered expandable metallic stent is a simple, safe, and effective procedure for closure of the BSF. Retrieval of the stent seems to be feasible. （J Intervent Radiol, 2007, 16： 253-257）