原发性肝癌蓖麻凝集素受体的观察

应用蓖麻凝集素(RCA)以ABC亲和细胞化学方法标记原发性肝癌组织,其RCA受体阳性率为86.4％(57/66),其中肝细胞癌63例,胆管上皮癌3例,其阳性率分别为85.7％(54/63)和100.0％(3/3),而对照组包括10例正常肝组织,9例肝炎,22例肝硬化的肝细胞均无RCA受体表达。作者认为,RCA受体出现是原发性肝癌重要标记之一。结果表明,RCA受体阳性率与肝细胞癌分化程度或排列方式无明显关系,但RCA受体分布型式与肿瘤分化程度似乎有一定关系,提永低分化肝细胞癌糖基可能更加紊乱。本文结果还显示,癌旁宿主部分肝细胞RCA受体呈阳性,这种化学结构异常先于形态学改变,对于认识癌前病变及诊断也有重要意义。     

100例肿瘤的凝集素受体观察

１００例肿瘤的凝集素受体观察江西省肿瘤医院（南昌市３３００２９）蔡勇涂侃黄传生陈玉君凝集素是一种能与糖类特异性结合并能使细胞凝集的蛋白质或糖蛋白。我们选用六种凝集素，对１００例不同类型的肿瘤进行亲合组化观察，探讨凝集素受体在肿瘤组织中的分布差异，为肿...     

乳腺癌凝集素受体的特异性及意义的探讨

Eight lectins were used to study 100 cases of breast carcinoma and 56 cases of non-cancerous breast tissues by lectin histochemical method. The results showed that Bandeiraea Simplicifolia (BSL) and Peanut agglutinin (PNA) had higher positive rates in breast carcinoma than both normal breast and benign breast lesions (P less than 0.005). The positive deposit in malignant lesions was mainly located in cytoplasm while in non-malignant lesions, it was almost always lined along the lumen of glands (P less than 0.005). The authors think that expression of PNA-receptor in the cytoplasm might be associated with the mechanism that the tumor could escape from immune attack. Comparative analysis on the normal breast indicated that PNA affinity histochemistry would be useful to the understanding of the metabolism of N-acetyl-D-galactosamine during the development of normal breast and histological origin of breast carcinoma.     

周围神经肿瘤,滑膜肉瘤的凝集素受体检测

应用ＡＢＣ免疫组化技术对４２例周围神经肿瘤和滑膜肉瘤进行１２处凝集素受体检测。结果：神经鞘瘤和神经纤维瘤ＰＮＡ和ＲＣＡ均阴性，恶性神经鞘瘤分别为５５％和１００％阳性。神经鞘瘤均表达ＰＨＡ，３３０％表达ＬＣＡ；神经纤维瘤均表达ＬＣＡ，５０％表达ＰＨＡ。滑膜肉瘤１５％表达ＲＣＡ，８５％表达ＷＧＡ；恶性神经鞘瘤均表达ＲＣＡ，不表达ＷＧＡ。提示：ＰＮＡ和ＲＣＡ尤其ＲＣＡ为鉴别周围神经肿瘤良恶性的标志。ＬＣ     

花生凝集素受体在子宫内膜癌中的定位研究

应用生物素交联的花生凝集素(PNA)对子宫内膜腺癌85例、正常子宫内膜8例、单纯型增生内膜7例、腺瘤型增生8例、不典型增生13例进行免疫组织化学研究。结果,PNA受体的阳性率在正常内膜、不同类型增生内膜及腺癌中逐渐增高,PNA受体的定位分布则从腺体的腔缘移向胞浆,RNA受体在子宫内膜腺癌中的过量表达和胞浆内的定位改变可能和内膜腺癌的发生及分化有关。     

阴茎良恶性病变中凝集素受体检测及意义

免疫化学酶标技术为一种敏感可靠的技术，本文通过对９２例阴茎良恶性肿瘤标本中药生凝集素受体及刀豆凝集素受体的分布进行观察，并结合临床资料及部分随访结果进行讨论，试图对阴茎良恶性肿瘤病变的监别及判定预后提供帮助。     

胆囊病变组织凝集素受体和癌胚抗原定位检测

应用ABC免疫组化技术对49例胆囊病变组织进行凝集素受体和癌胚抗原(CEA)的定位检测。结果表明胆囊癌的西非单叶豆(BSL)、双花藊豆(DBA)和扁豆(LCA)受体丢失,花生(PNA)受体和CEA表达明显增加,而且PNA受体与CEA表达呈正相关。     

大肠癌凝集素PNA,UEA—1及DBA受体的观察

凝集素作为糖蛋白组织化学探针已广泛应用于病理学研究。大肠癌及其癌前病变凝集素受体的研究近年来已有不少报道,各家所采用的凝集素种类、方法不同,对判断受体异常表达的标准也不完     

实体瘤诱导分化治疗研究现状

全反式维甲酸诱导分化治疗已成为急性早幼粒细胞白血病的首选治疗手段.随着对肿瘤诱导分化认识的不断深化,尤其是诱导分化在实体瘤动物实验中的治疗结果,提示该方法可能会成为人实体肿瘤治疗的又一突破点.     

Cellular Differentiation and Histogenesis of Rat Glandular Stomach Cancers

The gastric and intestinal phenotypic expressions of tumor cells in 18 adenomatous hyperplasias, 33 well-differentiated adenocarcinomas, and 16 undifferentiated adenocarcinomas (4 poorly differentiated adenocarcinomas, 10 signet-ring cell carcinomas and 2 mucinous adenocarcinomas) induced by N-methyl-N'-nitro-N-nitrosoguanidine or 4-nitroquinoline-I-oxide in the rat glandular stomach were studied by histochemical stainings for mucin and immunohistochemical staining for pepsinogen isozyme 1 (Pg 1). By histochemical staining for mucin [by the paradoxical concanavalin A method, the modified method with labeled peanut lectin, the galactose oxidase-Schiff (GOS) reaction, and the sialidase-GOS reaction] and immunohistochemical staining of Pg 1, gastric cancer cells of each histological group could be clearly classified into a gastric type, including mucous neck cell pyloric gland cell, and surface mucous cell subtypes, and an intestinal type, including goblet-cell, and intestinal absorptive cell subtypes. All tumors examined in this work consisted mainly of gastric-type cells but intestinal-type tumor cells were occasionally found among the gastric-type tumor cells. The incidences of intestinal-type cells in adenomatous hyperplasias (11.1%) and small well-differentiated adenocarcinomas (28.6%) were significantly less ( P <0.05) than that in large well-differentiated adenocarcinomas (68.4%). The incidence of intestinal-type cells in small undifferentiated adenocarcinomas (25.0%) was also less than that in large ones (58.3%). The present results suggest the occurrence of change of phenotypic expression of tumor cells from the gastric type to the intestinal type during growth of tumors.     

卵巢Brenner瘤的CT表现

目的 分析卵巢Brenner瘤的CT表现,帮助临床医生提高对卵巢Brenner瘤术前诊断的准确率.方法 收集1999年1月至2009年1月间经术后病理证实、有完整CT资料的卵巢Brenner瘤9例,其中良性8例,交界性1例.分析卵巢Brenner瘤的CT表现等.结果 9例卵巢Brenner瘤均为单侧发病,呈类球形、浅分叶状或不规则形,平均直径为7.8 cm,边界清楚.9例卵巢Brenner瘤中,单纯良性Brenner瘤5例,良性Brenner瘤伴有其他成分3例,交界性Brenner瘤1例.5例单纯良性Brenner瘤的CT表现均为实性或伴有小囊变,密度低于肌肉,其中2例伴有钙化,2例增强扫描后呈轻度不均匀强化.3例良性Brenner瘤伴有其他病理类型者的CT表现呈囊实性或囊性,囊实性成分分界清晰,实性成分密度亦低于肌肉,呈轻度强化,其中1例伴有钙化.1例交界性Brenner瘤的CT表现为不均匀实性肿块,密度高于肌肉,伴有大片低密度区及大块状钙化,增强扫描后呈明显不均匀强化.9例患者均无转移和胸腹水.结论 卵巢Brenner瘤常为单侧发病,合并其他肿瘤成分者多见.当肿瘤成分单一时,在影像学上常表现为实性,密度均匀或不均匀;当合并其他肿瘤成分时,可表现为囊实性或囊性,部分可伴钙化.在强化形式上,良性肿瘤呈轻度强化,交界性肿瘤呈中高度强化.     

血型抗原相关性凝集素受体在良恶性宫内膜组织的表达

荆豆素一1,兀鹰素—1—B4受体在增生期子宫内膜腺细胞几为阴性,内膜增生过长时开始出现腺细胞腔缘表达.内膜腺癌时细胞兀鹰素—1—B4在细胞腔缘结合较少或为不规则分布,荆豆素—1受体阳性普遍增强.双花扁豆素受体在增生期和增生过长腺体细胞可呈腔缘,胞浆和基底部混合分布,但腺癌仅见少数腺腔缘阳性细胞.花生素受体表达在不同性质内膜组织间差别不大.以上结果提示子宫内膜腺癌H型和A型血型抗原表达较之增生期和增生过长腺细胞分别有所增减.     

Dual Function of Macrophage Galactose/N-Acetylgalactosamine-specific Lectins: Glycoprotein Uptake and Tumoricidal Cellular Recognition

We investigated whether the interaction of peritoneal macrophages with extracellular ligands is mediated by C-type lectins specific for galactose and N-acetylgalactosamine. The carbohydrate-binding domain of mouse galactose/N-acetylgalactosamine-specific lectin was prepared in a recombinant form. The purified recombinant lectins were tested for competitive inhibition against glycoprotein uptake and against tumoricidal effect. Thioglycolate-elicited macrophages internalized galactosylated bovine serum albumin in vitro . The internalization was blocked by recombinant macrophage lectins. Activated macrophages obtained after intraperitoneal injection of a nonspecific immune potentiator, OK432, did not internalize galactosylated bovine serum albumin. These cells elicited a cytotoxic effect against P815 murine mastocytoma cells, and the effect was blocked by recombinant macrophage lectins. These results indicated that galactose/N-acetylgalactosamine-specific C-type lectins expressed on the surface of inflammatory macrophages and on activated tumoricidal macrophages mediate two distinct functions, i.e. glycoprotein uptake and tumoricidal effector mechanisms.     

卵巢印戒细胞间质瘤的临床病理分析

目的 :探讨卵巢印戒细胞间质瘤(signet-ring stromal tumor,SRST)的临床病理特点、鉴别诊断、治疗、生物学行为及预后。 方法 :采用HE、组织化学和免疫组织化学SP法检测SRST肿瘤细胞PAS-D、CK、CK7、CK20、Vi-mentin、Actin、inhibin、calretinin的表达。 结果 :4例SRST患者年龄20～56岁(平均40岁),所有肿瘤均为单侧发生。组织学表现为印戒样细胞与梭形细胞混合存在,无上皮分化(如腺管、细胞巢或条索)。组织化学染色PAS-D、免疫组织化学染色CK、CK7、CK20、inhibin、calretinin均为阴性,Vimentin和Actin阳性。患者随访11～52个月(平均27.8个月),均生存,无肿瘤复发或转移。 结论 :SRST是少见的卵巢良性肿瘤,预后好,组织学上与Krukenberg瘤等肿瘤易混淆,结合临床病理特征、组织化学和免疫组织化学有助于SRST的诊断。     

Peptide Hormones as Tumor Markers in Neuroendocrine Gastrointestinal Tumors

Since the development of the radioimmunoassay, the determination of serum or plasma levels of peptides has improved the diagnostic possibilities in neuroendocrine gastrointestinal and pancreatic tumors. Once the diagnosis of a carcinoid or an endocrine pancreatic tumor has been established the analyses of various peptides can help to characterize the tumor. Up till now there has been no 'screening' marker for the early detection of these tumors. the development of radioimmunoassay for analyses of chromogranins in plasma might imply such possibilities. in the management of the patients it is also important to have tumor markers that can give prognostic information. Recent findings might indicate that CA-50 could be an indicator of malignant disease and possibly also correlate with survival.