空军飞行员角膜内皮细胞的初步分析

Objegtive To preliminarily study the endothelial density baseline and the morphological characteristics of the corneal endothelial cell of Chinese Air Force pilot. Metheds Density and morphology of corneal endothelial cells were observed by Topcon-2000 noncontact specular microscope and analyzed by Imagenet 2000 software(version 2.53)on 56 pilots(112 eyes)While other 30 healthy men(60 eyes)who were with the comparable age and uncorrected visual acuity were compared and analyzed as the control group. Results The data showed that the average corneal endothelial cell density was 2797.0±330.3/mm2 in pilot group which was significantly lowet than 2917.57±318.04/mm2 in control group(t=2.286,P=0.024).The mean constituent ratio of hexagonal cell was 56.46±7.19 in pilot group compared with 60.33±7.68 in control group(t=8.253,P=O.001)and it was gradually reduced with the age growing in both groups(r=-0.395,-0.268,P=0.000.0.038).With the growing of flying hours the mean area of cell was increased under the eontrolled age factor according to partial correlation analysis(r=0.244,P=0.0 1).Conclusions Measuring of the corneaI endothelial cellular morphologic parameters and implementing corresponding analysis are helpful to know pilot's corneal endotheliurn changes in flight.     

空军飞行员角膜内皮细胞的初步分析

Objegtive To preliminarily study the endothelial density baseline and the morphological characteristics of the corneal endothelial cell of Chinese Air Force pilot. Metheds Density and morphology of corneal endothelial cells were observed by Topcon-2000 noncontact specular microscope and analyzed by Imagenet 2000 software(version 2.53)on 56 pilots(112 eyes)While other 30 healthy men(60 eyes)who were with the comparable age and uncorrected visual acuity were compared and analyzed as the control group. Results The data showed that the average corneal endothelial cell density was 2797.0±330.3/mm2 in pilot group which was significantly lowet than 2917.57±318.04/mm2 in control group(t=2.286,P=0.024).The mean constituent ratio of hexagonal cell was 56.46±7.19 in pilot group compared with 60.33±7.68 in control group(t=8.253,P=O.001)and it was gradually reduced with the age growing in both groups(r=-0.395,-0.268,P=0.000.0.038).With the growing of flying hours the mean area of cell was increased under the eontrolled age factor according to partial correlation analysis(r=0.244,P=0.0 1).Conclusions Measuring of the corneaI endothelial cellular morphologic parameters and implementing corresponding analysis are helpful to know pilot's corneal endotheliurn changes in flight.     

空军飞行员角膜内皮细胞的初步分析

Objegtive To preliminarily study the endothelial density baseline and the morphological characteristics of the corneal endothelial cell of Chinese Air Force pilot. Metheds Density and morphology of corneal endothelial cells were observed by Topcon-2000 noncontact specular microscope and analyzed by Imagenet 2000 software(version 2.53)on 56 pilots(112 eyes)While other 30 healthy men(60 eyes)who were with the comparable age and uncorrected visual acuity were compared and analyzed as the control group. Results The data showed that the average corneal endothelial cell density was 2797.0±330.3/mm2 in pilot group which was significantly lowet than 2917.57±318.04/mm2 in control group(t=2.286,P=0.024).The mean constituent ratio of hexagonal cell was 56.46±7.19 in pilot group compared with 60.33±7.68 in control group(t=8.253,P=O.001)and it was gradually reduced with the age growing in both groups(r=-0.395,-0.268,P=0.000.0.038).With the growing of flying hours the mean area of cell was increased under the eontrolled age factor according to partial correlation analysis(r=0.244,P=0.0 1).Conclusions Measuring of the corneaI endothelial cellular morphologic parameters and implementing corresponding analysis are helpful to know pilot's corneal endotheliurn changes in flight.     

空军飞行员角膜内皮细胞的初步分析

Objegtive To preliminarily study the endothelial density baseline and the morphological characteristics of the corneal endothelial cell of Chinese Air Force pilot. Metheds Density and morphology of corneal endothelial cells were observed by Topcon-2000 noncontact specular microscope and analyzed by Imagenet 2000 software(version 2.53)on 56 pilots(112 eyes)While other 30 healthy men(60 eyes)who were with the comparable age and uncorrected visual acuity were compared and analyzed as the control group. Results The data showed that the average corneal endothelial cell density was 2797.0±330.3/mm2 in pilot group which was significantly lowet than 2917.57±318.04/mm2 in control group(t=2.286,P=0.024).The mean constituent ratio of hexagonal cell was 56.46±7.19 in pilot group compared with 60.33±7.68 in control group(t=8.253,P=O.001)and it was gradually reduced with the age growing in both groups(r=-0.395,-0.268,P=0.000.0.038).With the growing of flying hours the mean area of cell was increased under the eontrolled age factor according to partial correlation analysis(r=0.244,P=0.0 1).Conclusions Measuring of the corneaI endothelial cellular morphologic parameters and implementing corresponding analysis are helpful to know pilot's corneal endotheliurn changes in flight.     

空军飞行员角膜内皮细胞的初步分析

Objegtive To preliminarily study the endothelial density baseline and the morphological characteristics of the corneal endothelial cell of Chinese Air Force pilot. Metheds Density and morphology of corneal endothelial cells were observed by Topcon-2000 noncontact specular microscope and analyzed by Imagenet 2000 software(version 2.53)on 56 pilots(112 eyes)While other 30 healthy men(60 eyes)who were with the comparable age and uncorrected visual acuity were compared and analyzed as the control group. Results The data showed that the average corneal endothelial cell density was 2797.0±330.3/mm2 in pilot group which was significantly lowet than 2917.57±318.04/mm2 in control group(t=2.286,P=0.024).The mean constituent ratio of hexagonal cell was 56.46±7.19 in pilot group compared with 60.33±7.68 in control group(t=8.253,P=O.001)and it was gradually reduced with the age growing in both groups(r=-0.395,-0.268,P=0.000.0.038).With the growing of flying hours the mean area of cell was increased under the eontrolled age factor according to partial correlation analysis(r=0.244,P=0.0 1).Conclusions Measuring of the corneaI endothelial cellular morphologic parameters and implementing corresponding analysis are helpful to know pilot's corneal endotheliurn changes in flight.     

空军飞行员角膜内皮细胞的初步分析

Objegtive To preliminarily study the endothelial density baseline and the morphological characteristics of the corneal endothelial cell of Chinese Air Force pilot. Metheds Density and morphology of corneal endothelial cells were observed by Topcon-2000 noncontact specular microscope and analyzed by Imagenet 2000 software(version 2.53)on 56 pilots(112 eyes)While other 30 healthy men(60 eyes)who were with the comparable age and uncorrected visual acuity were compared and analyzed as the control group. Results The data showed that the average corneal endothelial cell density was 2797.0±330.3/mm2 in pilot group which was significantly lowet than 2917.57±318.04/mm2 in control group(t=2.286,P=0.024).The mean constituent ratio of hexagonal cell was 56.46±7.19 in pilot group compared with 60.33±7.68 in control group(t=8.253,P=O.001)and it was gradually reduced with the age growing in both groups(r=-0.395,-0.268,P=0.000.0.038).With the growing of flying hours the mean area of cell was increased under the eontrolled age factor according to partial correlation analysis(r=0.244,P=0.0 1).Conclusions Measuring of the corneaI endothelial cellular morphologic parameters and implementing corresponding analysis are helpful to know pilot's corneal endotheliurn changes in flight.     

空军飞行员角膜内皮细胞的初步分析

Objegtive To preliminarily study the endothelial density baseline and the morphological characteristics of the corneal endothelial cell of Chinese Air Force pilot. Metheds Density and morphology of corneal endothelial cells were observed by Topcon-2000 noncontact specular microscope and analyzed by Imagenet 2000 software(version 2.53)on 56 pilots(112 eyes)While other 30 healthy men(60 eyes)who were with the comparable age and uncorrected visual acuity were compared and analyzed as the control group. Results The data showed that the average corneal endothelial cell density was 2797.0±330.3/mm2 in pilot group which was significantly lowet than 2917.57±318.04/mm2 in control group(t=2.286,P=0.024).The mean constituent ratio of hexagonal cell was 56.46±7.19 in pilot group compared with 60.33±7.68 in control group(t=8.253,P=O.001)and it was gradually reduced with the age growing in both groups(r=-0.395,-0.268,P=0.000.0.038).With the growing of flying hours the mean area of cell was increased under the eontrolled age factor according to partial correlation analysis(r=0.244,P=0.0 1).Conclusions Measuring of the corneaI endothelial cellular morphologic parameters and implementing corresponding analysis are helpful to know pilot's corneal endotheliurn changes in flight.     

空军飞行员角膜内皮细胞的初步分析

Objegtive To preliminarily study the endothelial density baseline and the morphological characteristics of the corneal endothelial cell of Chinese Air Force pilot. Metheds Density and morphology of corneal endothelial cells were observed by Topcon-2000 noncontact specular microscope and analyzed by Imagenet 2000 software(version 2.53)on 56 pilots(112 eyes)While other 30 healthy men(60 eyes)who were with the comparable age and uncorrected visual acuity were compared and analyzed as the control group. Results The data showed that the average corneal endothelial cell density was 2797.0±330.3/mm2 in pilot group which was significantly lowet than 2917.57±318.04/mm2 in control group(t=2.286,P=0.024).The mean constituent ratio of hexagonal cell was 56.46±7.19 in pilot group compared with 60.33±7.68 in control group(t=8.253,P=O.001)and it was gradually reduced with the age growing in both groups(r=-0.395,-0.268,P=0.000.0.038).With the growing of flying hours the mean area of cell was increased under the eontrolled age factor according to partial correlation analysis(r=0.244,P=0.0 1).Conclusions Measuring of the corneaI endothelial cellular morphologic parameters and implementing corresponding analysis are helpful to know pilot's corneal endotheliurn changes in flight.     

空军飞行员角膜内皮细胞的初步分析

Objegtive To preliminarily study the endothelial density baseline and the morphological characteristics of the corneal endothelial cell of Chinese Air Force pilot. Metheds Density and morphology of corneal endothelial cells were observed by Topcon-2000 noncontact specular microscope and analyzed by Imagenet 2000 software(version 2.53)on 56 pilots(112 eyes)While other 30 healthy men(60 eyes)who were with the comparable age and uncorrected visual acuity were compared and analyzed as the control group. Results The data showed that the average corneal endothelial cell density was 2797.0±330.3/mm2 in pilot group which was significantly lowet than 2917.57±318.04/mm2 in control group(t=2.286,P=0.024).The mean constituent ratio of hexagonal cell was 56.46±7.19 in pilot group compared with 60.33±7.68 in control group(t=8.253,P=O.001)and it was gradually reduced with the age growing in both groups(r=-0.395,-0.268,P=0.000.0.038).With the growing of flying hours the mean area of cell was increased under the eontrolled age factor according to partial correlation analysis(r=0.244,P=0.0 1).Conclusions Measuring of the corneaI endothelial cellular morphologic parameters and implementing corresponding analysis are helpful to know pilot's corneal endotheliurn changes in flight.     

吸低氧混合气后循环内皮细胞数目及形态学的变化

在吸１０．５％的低氧混合气１５ｍｉｎ前后观察了６０名战斗机飞行员循环内皮细胞数目的变化和形态学的改变，结果实验前的数目为１．０３±０．４８（个／μｌ）；实验后３．７３±０．５７（个／μｌ）发生明显改变。并观察到实验后年轻内皮细胞的脱落明显增多，与飞行员生活习惯有一定的相关性。结果表明：在急性呼吸低氧以后的对血内皮细胞造成一定的损害。这可心来用来解释飞行员的职业病。     

电场对血管内皮细胞增殖及其细胞周期的影响

目的 观察电场对血管内皮细胞增殖的影响并探讨其作用机制。方法 将血管内皮细胞分别暴露于场强为50mVmm^-1,100mVmm^-1和200mVmm^-1的电场7。部分细胞接受细胞凋亡抑制剂Z－VAD－FMK的同时暴露于200mVmm^-1的电场。计算细胞密度，生长率和分裂指数，行细胞凋亡信号蛋白caspase-3免疫荧光染色，以流式细胞仪行细胞周期时相分析和通过蛋白印记分析检测细胞周期调节蛋白的表达。结果 50mVmm^-1或100mVmm^-1的电场暴露对细胞增殖没有明显影响，但200mVmm^-1的电场细胞增殖明显受抑，表现为细胞密度，生长率及分裂指数明显下降。Z－VAD－FMK未能防止电场暴露所诱发的细胞增殖的抑制。电场暴露细胞未见有明显的caspase-3阳性染色。电场暴露细胞的细胞周期时相图上，亦未见提示细胞凋亡的亚G1峰，而显示其细胞周期的G1／S过渡受抑。电场暴露细胞的Cdk2表达不受影响，但Cyclin-E的表达明显降低，而cyclin-E/Cdk2复合物的抑制蛋白p27^kip1的表达则明显增加。结论 电场暴露通过诱发细胞周期停滞而非细胞凋亡抑制血管内皮细胞的增殖，该作用是籍增加p27^kip1的表达和减少cyclin-E的表达而实现。