猪囊尾蚴特异循环免疫复合物致病作用的研究

本文对脑型和混合型猪囊尾蚴病患者脑脊液（ＣＳＦ）中特异循环免疫复合物（ＣＩＣ）进行了检测。其结果与患者ＣＴ表现及吡喹酮治疗第一个疗程过程中患者出现的高颅压情况存在一定联系。人工免疫复合物（ＩＣ）对ＢＡＬＢ／ｃ鼠作用后进行病理检查，发现脑实质和肾脏小血管均出现充血现象，部分小血管内皮细胞遭到破坏。因此认为特异循环免疫复合物可能是脑型、混合型猪囊尾蚴病患者在第一疗程中出现高颅压反应的主要因素之一。     

IFAT和McAb—LAT诊断囊虫病的诊断

本文采用猪囊尾蚴冰冻切片抗原间接荧光抗体试验（ＩＦＡＴ）和抗猪囊尾蚴单克隆抗体乳胶凝集试验（ＭｃＡｂ－ＬＡＴ）对囊虫病患者进行特异性抗体和抗原的检测，囊虫病患者抗体检出率为８３．７１％，分型抗体检测，混合型及脑型检出率明显高于皮肌型（Ｐ〉０．０１）；患者循环抗原的检出率为７３．０５％，脑型患者囊虫活动期的循环抗原的阳性率明显高于囊虫钙化期（Ｐ〈０．０１），药物治疗３个月以上患者循环抗原的阳性率降为     

冠心病患者红细胞免疫功能的变化

本文测定了正常人及冠心病患者的红细胞免疫功能。结果表明，冠心病患者的红细胞Ｃ３ｂ受体花环率（ＲＢＣ－Ｃ３ｂＲＲ）降低，红细胞免疫复合物花环率（ＲＢＣ－ＩＣＲ）及循环免疫复合物（ＣＩＣ）增高；且ＣＩＣ与ＲＢＣ－Ｃ３ｂＲＲ呈负相关，与ＲＢＣ－ＩＣＲ呈正相关；ＲＢＣ－Ｃ３ｂＲＲ与ＲＢＣ－ＩＣＲ呈负相关，提示冠心病患者存在红细胞免疫功能低下，可能为继发性改变。     

IFAT和McAb-LAT诊断囊虫病的研究

本文采用猪囊尾蚴冰冻切片抗原间接荧光抗体试验（ＩＦＡＴ）和抗猪囊尾蚴单克隆抗体胶乳凝集试验（ＭｃＡｂ－ＬＡＴ）对囊虫病患者进行特异性抗体和抗原的检测，囊虫病患者抗体检出率为８３．７１％，分型抗体检测，混合型及脑型检出率明显高于皮肌型（Ｐ＜０．０１）；患者循环抗原的检出率为７３．０５％，脑型患者囊虫活动期的循环抗原的阳性率明显高于囊虫钙化期（Ｐ＜０．０１），药物治疗３个月以上患者循环抗原的阳性率降为４４．１１％。ＩＦＡＴ和ＭｃＡｂ－ＬＡＴ在囊虫病临床诊断中具有较高价值，循环抗原的检测在脑型囊虫病活动期诊断和疗效考核中具有重要意义     

应用特异性单克隆抗体测定脑囊虫病患者血清及脑脊液中循…

应用特异单克隆抗体作ＤＯＴ－ＥＬＩＳ和竞争ＥＬＩＳＡ检测６５例脑囊虫病患者血清和脑脊液中前淳离循环Ａｇ（ＦＣＡｇ）和循环免疫复合物抗原（ＣＩＣＡｇ）。血清ＦＣＡｇ阳性率分别为８１．３％和８１．５％，ＣＩＣＡｇ阳性率为９５．４％和９２．３％（０．１９－１２０．０ｇ／ｍｌ）脑脊液测定ＦＣＡｇ阳性率为７８．５％和８６．２％，ＣＩＣＡｇ阳率率为９０．８％和９３．８％。结果表明检测总循环抗的有利于提高脑囊虫     

检测血清和脑脊液中囊尾蚴循环抗原诊断脑囊虫病的研究

目的：用抗血清检测脑囊虫病患者血清和脑脊液（ Ｃ Ｓ Ｆ）中囊尾蚴循环抗原（ Ｃ Ａｇ）诊断脑囊虫病。方法：用 Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ提纯的蛋白质分子量为 ６４ ｋ Ｄａ、５３ ｋ Ｄａ、３２ ｋ Ｄａ～３０ ｋ Ｄａ 的囊尾蚴抗原分别免疫家兔,制备相应的抗血清,以双夹心 Ｅ Ｌ Ｉ Ｓ Ａ 检测患者血清和 Ｃ Ｓ Ｆ中 Ｃ Ａｇ。结果：抗５３ ｋ Ｄａ 抗原抗血清对 ３２ 例脑囊虫病活动型患者血清和 Ｃ Ｓ Ｆ中 Ｃ Ａｇ 的检出率分别为９３．８％ 和９１．７％ ,１６ 例脑囊虫病非活动型患者仅１ 例 Ｃ Ｓ Ｆ Ｃ Ａｇ 阳性。 Ｃ Ａｇ 检出率明显高于用抗６４ ｋ Ｄａ、３２ ｋ Ｄａ～３０ ｋ Ｄａ 囊尾蚴抗原抗血清检测的结果（ Ｐ＜ ０．０５）。结论：抗５３ ｋ Ｄａ 囊尾蚴抗原抗血清检测活动型脑囊虫病患者血清和 Ｃ Ｓ Ｆ中的 Ｃ Ａｇ 敏感性较高,特异性较强,可用于活动型脑囊虫病的诊断和疗效考核。     

231例脑囊虫病患者脑脊液中循环抗原检测分析

本文报告应用抗猪囊尾蚴单克隆抗体４Ｆ８－ＥＬＩＳＡ对２３１例脑囊虫病患者脑脊液中循环抗原检测的结果。经与患者病史、临床分型和脑ＣＴ检查结果比较，发现脑脊液中ＣＡｇ与有无绦虫史无关。脑型合并皮下结节患者脑脊液中ＣＡｇ的阳性率明显高于单纯型脑囊虫病和皮下结节消失组的患者。ＣＡｇ检测的结果与囊尾蚴和脑部寄生的数量，病灶的新旧与部位等病理情况有关。表明ＭｃＡｂ（４Ｆ８）－ＥＬＩＳＡ不仅可用于脑囊虫病的诊断     

猪囊尾蚴抗原诱生肿瘤坏死因子(TNF)和一氧化氮(NO)的观察

本文应用猪囊尾蚴囊液抗原刺激Ｃ５７小鼠腹腔巨噬细胞，诱生肿瘤坏死因子（ＴＮＦ）和一氧化氮（ＮＯ）。结果表明，抗原浓度在１０、５０和１００μｇ／ｍｌ分别刺激８、４和１ｈ即可检测出ＴＮＦ和ＮＯ，并且其活性随抗原浓度的增加、刺激时间的延长而增加。检测人体猪囊尾蚴结节１９个，每个囊结周围的巨噬细胞分泌ＴＮＦ和ＮＯ的阳性率为１００％。检测脑型和混合型猪囊尾蚴患者血清４１份，ＴＮＦ和ＮＯ阳性率分别为３９．０％（１６／４１）和４１．５％（１７／４１）；ＣＳＦ１５份，ＴＮＦ和ＮＯ的阳性率均为２６．７％（４／１５）。认为ＴＮＦ和ＮＯ可能为寄生虫感染早期的主要免疫保护因素，并参与带虫免疫。ＣＳＦ中ＮＯ的升高可能与脑囊虫患者的神经系统症状有关。     

吡喹酮对疑似脑囊尾蚴病患者诊疗作用的研究

目的观察吡喹酮（PQT）对疑似脑囊尾蚴病患者的诊疗作用。方法先采用间接血凝试验（IHA）和酶联免疫吸附试验（ELISA）检测疑似脑囊尾蚴病患者血清和脑脊液中的抗体,并采用夹心ELISA法检测患者血清和脑脊液中的循环抗原,再应用大剂量PQT[50 mg/（kg.d）×12 d]对疑似脑囊尾蚴病患者进行诊断性治疗。结果93例疑似脑囊尾蚴病患者诊断性治疗后有38例确诊患脑囊尾蚴病,42例基本确诊患其他脑部疾患,13例仍诊断不明。38例脑囊尾蚴病患者有27例服吡喹酮后出现杀虫反应,其中治疗后有17例患者复查脑CT或MR I,结果显示病灶缩小、周围水肿吸收或消失;有2例患者复查脑CT显示病灶增多,且呈囊性影像学表现;另11例确诊为脑囊尾蚴病的患者服药期间未出现杀虫反应,根据治疗后复查CT或MR I,病灶较治疗前吸收或消失而确诊。确诊的38例脑囊尾蚴病患者再经过1～2个疗程抗囊尾蚴治疗后临床症状消失,其中31例患者复查脑CT,病灶完全吸收者26例,病灶出现钙化者5例。结论大剂量吡喹酮对疑似脑囊尾蚴病患者具有较好的诊疗作用。     

检测血清和脑脊液中囊尾蚴循环抗原诊断脑囊虫 …

目的：用抗血清检测脑囊虫病患者血清和脑脊液（ＣＳＦ）中囊尾蚴循环抗原（ＣＡｇ）诊断脑囊虫病。方法：用ＳＤＳ－ＰＡＧＥ提纯的蛋白质分子量为６４ｋＤａ、５３ｋＤａ、３２ｋＤａ～３０ｋＤａ的囊尾蚴抗原分别免疫家兔,制备相应的抗血清,以双夹心ＥＬＩＳＡ 检测患者血清和ＣＳＦ中ＣＡｇ。结果：抗原体５３ｋＤａ抗原抗血清对３２例脑囊虫病活动型患者血清和ＣＳＦ中ＣＡｇ的检出率分别为９３．８％和９１．７％,１６例脑     

近期感染与脑梗死发病的相关性研究

目的:探讨近期感染与脑梗死发病的关系,分析循环免疫复合物(circulatingimmunecomplex,CIC)、C反应蛋白(Creactiveprotein,CRP)、补体C4、C3、C1q与近期感染和脑梗死发病的关联。方法:调查了97例脑梗死患者与对照组83例,测定26例有近期感染史和71例无近期感染史的脑梗死病例及83例对照组的血清CIC、CRP、C4、C3、C1q含量,并进行分析。结果:经单因素Logistic回归显示:近期感染(OR,2.67;95%可信区间,1.2～5.9),CIC(OR,1.08;95%可信区间,1.03～1.12),CRP(OR,1.27;95%可信区间,1.16～1.39)水平升高,C1q(OR,0.88;95%可信区间,0.83～0.93),C3(OR,0.98;95%可信区间,0.96～0.99),C4(OR,0.93;95%可信区间,0.89～0.97)水平降低都是脑梗死的危险因素。但经多因素Logistic回归后仅CRP(OR,1.26;95%可信区间,1.12～1.43)水平升高,C1q(OR,0.88;95%可信区间,0.82～0.94),C3(OR,0.98;95%可信区间,0.96～0.99)水平降低是脑梗死的危险因素。经Spearman相关分析显示:感染与CIC有关(r=0.27,P=0.0008),与脑梗死发作有关(r=0.18,P=0.013)。脑梗死与CIC(r=0.3,P<0.0001),CRP(r=0.43,P<0.0001),C1q(r=-0.37,P=0.0001),C3(r=-0.26,P=0.0007),C4(r=-0.27,P=0.0004)水平有关。结论:感染是脑梗死的一个危险因素;循环免疫复合物CIC、CRP、补体C4、C3、C1q参与了脑梗死发病;循环免疫复合物既与感染有关联又参与了脑梗死的免疫损伤过程。     

单克隆抗体检测血吸虫病人血清特异性循环免疫复合物的初步探讨

本文系探讨用ⅢD_(10)McAb识别18例慢性血吸虫病患者血清CIC,并与经聚乙二醇(PEG)沉淀的CIC对照,二者结果相似.该McAb-ELISA方法检测血吸虫病慢性患者67例,46例呈阳性反应,21例呈阴性反应而对肺吸虫病患者59例、健康人23例全部呈阴性反应.初步结果表明,该方法检测血清CIC具有较高的特异性.     

Circulating immune complexes in Hodgkin's disease

Levels of circulating immune complexes (CIC) were estimated in the sera of 110 patients with Hodgkin's disease (HD) and of 103 normal subjects by polyethylene glycol precipitation method. Levels of CIC were significantly elevated in HD patients compared to normal subjects with 63% of the patients showing levels above 95th percentile of the normal. Patients with symptoms showed significantly elevated levels of CIC compared to patients without symptoms. There was a good correlation between the levels of CIC and the clinical stages, the former increasing from stage I to IV. However, no such correlation was noticed with respect to the histological type of the disease. On polyacrylamide gel electrophoresis CIC of normal subjects as well as of HD patients showed a number of protein bands. But CIC of HD patients had one additional band with approximate molecular weight of 130 000 which was absent in CIC of normal subjects.     

Analysis of circulating immune complexes from patients with ankylosing spondylitis by gel electrophoresis and immunoblotting using antiserum against a psoriasis associated retrovirus-like particle.

Circulating immune complexes (CIC) were isolated from patients with ankylosing spondylitis (AS) and healthy blood donors by isopycnic ultracentrifugation in sucrose gradients. The CIC were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The major components of the CIC were identified as albumin, immunoglobulins, and complement factors. A 70 kD component and several low molecular weight components (Mr 19 kD and 14 kD (doublet] were detectable only in CIC from patients with AS. An antiserum raised against the envelope glycoprotein, gp70, of a psoriasis associated retrovirus-like particle was applied to check for cross reacting activity. This antiserum reacted with both a 70 kD and a 40-45 kD component in CIC from three out of six patients but not with CIC from any of the blood donors.     

Circulating immune complexes and apolipoproteins in ischemic heart disease

Levels of circulating immune complexes (CIC) and apolipoproteins (apo-A and apo-B) were compared and correlated with the severity of atherosclerotic affection of heart vessels in coronary patients with coronarographically-verified coronary arterial atherosclerosis. An inverse correlation between blood CIC and apo-A levels, and a direct correlation between the CIC level and the apo-B/apo-A index were demonstrated. The variation of CIC levels in patients with atherosclerotic lesions of varying severity corresponded to that of the apo-B/apo-A index. The correlation of these parameters was particularly significant in coronary patients with 2 coronary vessels affected.     

感染诱发的脑梗死发作时的循环免疫复合物

目的 分析循环免疫复合物(CIC)在脑梗死发作时的变化及其与近期感染的关系。方法测定46例动脉硬化性脑梗死病例发病初期和恢复期及124例正常对照的CIC、补体Clq、C3,脑梗死组病例做发病初期和恢复期的先后比较,同时与正常对照测定值比较。结果 脑梗死组发病初期CIC测定值(16.47±12.46mg/dl),高于正常对照组(6.70±4.27mg/dl,P<0.01),井在恢复期下降,Clq发病初期(0.14±0.04g/L)低于恢复期(0.17±0.04g/L,P<0.05)并低于正常对照(0.20±0.04g/L),C3(1.16±0.06g/L)低于正常对照组(1.71±0.57g/L)且先后两期测定变化无差异。按有/无感染史分两组比较,有感染史组梗死初期CIC高于无感染史组并持续到恢复期,Clq测定值两组均偏低,并在恢复期回升明显,有感染史组C3高于无感染史组,回升不明显。CIC与梗死的最大直径和面积呈一定正相关。结论 CIC升高可能与感染有关,梗死初期CIC与脑梗死损伤程度呈一定正相关。     

慢性特发性便秘发病机制探讨

目的通过结肠、肛门直肠动力学的变化及心理学基础初步探讨慢性特发性便秘(CIC)可能的发病机制.方法用肛门直肠测压法检测21例CIC患者肛门直肠动力学的变化,同时进行心理测试.20名健康者作对照.CIC组中11例行远端结肠测压,9例非CIC组作对照.结果CIC患者年龄偏大(P＜0.05);肛门括约肌静息压、最大缩榨压降低(P＜0.025,P＜0.005);肛管高压带长度增加(P＜0.005);引起直肠肛门抑制反射的最小松弛容量(MVR)增加(P＜0.005),肛门括约肌松弛率下降(P＜0.025);直肠内部容量刺激的排便阈值和最大耐受量均明显增加(P＜0.005,P＜0.005);11例CIC组患者远端结肠测压结果表明收缩时间百分比、动力指数较非CIC组均明显降低(P＜0.005);CIC患者焦虑、抑郁精神心理异常倾向的出现明显高于对照组(P＜0.05,P＜0.01).结论CIC发病机制是复杂的,结肠、肛门直肠动力学及精神心理因素均参与发病.     

C3-containing serum immune complexes in patients with systemic lupus erythematosus: correlation to disease activity and comparison with other rheumatic diseases

Summary Although testing for circulating immune complexes (CIC) is regarded as a useful, complementary, laboratory parameter in the differential diagnosis and management of immune complex-induced vasculitis syndromes, there is still an uncertainty with regard to assay systems used for the demonstration of soluble immune complexes. This is partly due to difficulties in the reproducibility, handling and principle limitations of available test systems for the assessment of soluble immune complexes in body fluids. In the present communication a modification of the anti-C3 test for the determination of CIC was developed using nitrocellulose as a solid phase matrix. IgG-, IgA- and IgM-containing CIC were determined and quantified using standard immune complex preparations. When 39 sera of SLE patients, 12 sera of patients with vascultis syndromes, 10 sera of rheumatoid arthritis patients and 11 sera of patients with ankylosing spondylitis were tested, predominantly IgG-containing CIC could be demonstrated. Only in SLE patients was a significant amount of other immunoglobulin isotypes detected in CIC. In these patients a significant difference of IgG-containing CIC levels was found with regard to patients with high and low disease activity (P<0.0001). A significant correlation was also established between IgG-containing CIC and anti-dsDNA antibodies (P<0.001). In a longitudinal study the isotypes in the isolated CIC were found to be constant.     

Bacteriuria in patients treated with clean intermittent catheterization.

Bacteriuria has been studied in 407 patients treated with clean intermittent catheterization (CIC) during 1 year. Significant bacteriuria was found in 50.6% of 1413 analyzed urine samples. Escherichia coli was the dominating species (54.8%). The relative distribution of species was different in males and females, but there were no differences between the CIC patients and a reference group of outpatients. On the other hand, a higher frequency of resistance among enterobacteria was found in samples from CIC patients compared to the reference group. The majority of CIC patients with bacteriuria had no symptoms, and bacteriuria per se does not seem to be an indication for treatment in most of these patients.     

Circulating immune complexes in human fascioliasis. Relationship with Fasciola hepatica egg output

Circulating immune complexes were investigated by the 125I-Clq binding test in serum from patients with fascioliasis. Only 36% of all the patients studied showed significant levels of CIC. Nevertheless, when we considered only the patients eliminating Fasciola hepatica eggs in the stool and/or with the acute phase of the infection, the detection of CIC was very higher (more than 70% of the cases). In addition, a close relationship was observed between F. hepatica egg output and the incidence of CIC. This data suggest strongly the occurrence of specific parasite antigens in the detected CIC. The involvement of CIC in the pathogenesis of the acute hepatic fascioliasis is discussed.