非淋菌性宫颈炎174例临床分析

目的探讨非淋菌性宫颈炎的诊断和治疗。方法对2009年9月～2010年3月174例非淋菌性宫颈炎患者的临床资料进行回顾性分析。结果 174例中支原体感染76例,占43.68;沙眼衣原体感染42例,占24.14;两者混合感染56例,占32.18;大多数患者宫颈口有白色稀薄分泌物流出,94例有宫颈管接触性出血,占54.02;阿奇霉素治愈率65.52;多西环素联合阿奇霉素治愈率86.21%。结论对久治不愈的妇科炎症患者其宫颈口有白色稀薄分泌物流出及颈管内触血者,应行支原体、衣原体检测,尽早明确诊断。治疗药物是多西环素联合阿齐霉素优于单一阿奇霉素。     

阿奇霉素联合碘甘油治疗非淋菌性宫颈炎疗效观察

目的观察阿奇霉素联合碘甘油治疗支原体、沙眼衣原体引起的非淋菌性宫颈炎临床疗效。方法采用随机对比方法,选择门诊确诊为支原体感染、沙眼衣原体感染或二者混合性感染引起非淋菌性宫颈炎的患者115例作为研究对象,分成两组,对照组及治疗组。对照组55例,阿奇霉素每次0.5g加入注射液中静脉滴注,每日1次,连用5d。治疗组60例,同以上静脉给药加局部用药,1%碘甘油涂抹宫颈管和宫颈表面糜烂处,每日1次,共7次。两组治疗结束后1～2周非月经期复查。结果对照组:治愈率36.4%,总有效率78.2%,病原清除率63.7%;治疗组:治愈率63.3%,总有效率91.6%,病原清除率83.3%。两组治愈率相比较(χ2=8.92,P0.01),总有效率相比较(χ2=4.28,P0.05),病原清除率相比较(χ2=6.52,P0.05),差异均有统计学意义。结论阿奇霉素联合碘甘油治疗支原体、沙眼衣原体引起的非淋菌性宫颈炎疗效显著,能有效清除病原体、清除局部炎症,提高治愈率。     

沙眼衣原体宫颈炎的临床治疗观察

目的:观察阿奇霉素治疗沙眼衣原体宫颈炎的疗效。方法:80例患者取宫颈粘液做衣原体酶免疫试验,排除淋球菌和解脲支原体感染;给予阿奇霉素1.0g,1d1次,口服,给予10d治疗。结果:76例治愈,治愈率为95%。结论:阿奇霉素为治疗沙眼衣原体宫颈炎安全可靠的药物。     

阿奇霉素治疗无并发症淋病和非淋菌性尿道炎的疗效观察

目的：用阿奇霉素治疗无并发症淋病和由沙肯衣原体,解脲支原体引直的非淋菌性尿道炎的疗效。方法：给药方法为单剂量阿奇霉素１．０ｇ顿服,７天后进行疗效评价。结果：６７例病人中无并发症淋病组,非淋菌性尿道炎组,混合感染组的治愈率分别为１００％、８３％、５４％,对淋球菌、沙眼衣原体、解脲支原体的清除率分别为９１％、１００％、６９％,不良支应发生率９．０％,结论：表明阿奇霉素治疗由淋菌、沙眼衣原体、解脲支原体引起的无并发症淋病和非淋菌性尿道炎的疗效确切,且用药方便,不良反应少而轻微。     

150例非淋菌性尿道炎病原学检测

目的 对有关非淋菌性尿道炎的病原学进行探讨。方法 应用培养法和衣原体抗原快速免疫测定法对150例非淋菌性尿道炎患者的尿道或宫颈分泌物进行检测。结果 沙眼衣原体阳性32例（21．33％）,解脲支原体阳性35例（23．33％）,白色念珠菌阳性18例（12．00％）,人型支原体阳性1例（0．67％）,白色葡萄球菌阳性5例（3．33％）,其它细菌阳性9例（6．00％）,混合感染阳性50例（33．33％） ,其中解以原体、人型支原体、沙眼衣原体均阳性14例（9．33％）。结论 非淋菌性尿道炎的主要病原体是沙眼衣原体和解脲支原体,混合感染以解脲支原体、人型支原体、沙眼衣原体均为阳性为主。     

阿奇霉素治疗非淋菌性尿道炎,宫颈炎76例

目的：评估阿奇霉素对沙眼衣原体和解脲支原体泌尿生殖道感染的疗效。方法：用阿奇霉素治疗非淋菌性尿道炎、宫颈炎７６例。结果：沙眼衣原体感染痊愈率９３８％（３０／３２），总有效率１００％（３２／３２）；解脲支原体痊愈率８４１％（３７／４４），总有效率９０９％（４０／４４）。无合并症者只需１０ｇ单剂给药，副作用少而且轻微。结论：阿奇霉素对２种病原体均具较好疗效，可作为泌尿生殖道沙眼衣原体感染的首选药物，亦可选用于对解脲支原体的治疗。     

218例宫颈炎症与生殖道支原体和衣原体感染检测分析

目的探讨妇科宫颈炎症患者与解脲支原体（UU）和沙眼衣原体（CT）感染的关系。方法对218例宫颈炎症患者做支原体（培养加药敏）和沙眼衣原体（用检测试剂盒）检测分析。结果1．在218例患者中，肉眼见到脓性或黏液脓性分泌物患者162例（74．31％）；出现宫颈管内出血患者111例（50．92％）；实际阳性病例138例（63．30％），UU阳性114例（52．29％），CT阳性69例（31．65％），其中有45例双重感染（20．64％）。2．生殖道支原体衣原体阳性率与患者年龄、职业、文化程度、孕产次及有无计划生育手术史均有一定的关系。结论生殖道支原体衣原体检测在妇科门诊中具有十分重要的价值，对提高妇女生殖健康水平、防止性传播疾病蔓延起到积极的作用。     

非淋菌性阴道炎感染沙眼衣原体及支原体98例检测分析

目的探讨非淋菌性阴道炎患者与宫颈沙眼衣原体及支原体感染的相关性。方法取宫颈分泌物进行沙眼衣原体（CT）及支原体（UU）检测，CT采用抗原免疫法；UU采用培养法。结果98例中，CT和UU的检出率分别为23．5％和31．4％，两者感染的总阳性率为54．9％，两者混合感染率为9．6％。结论提示沙眼衣原体及支原体感染为非淋菌性阴道炎的重要致病因素，对非淋菌性阴道炎患者应重视进行CT和UU的检测。     

克拉霉素联合阴道局部应用强力霉素治疗非淋菌性宫颈炎疗效观察

目的:探讨克拉霉素联合阴道局部应用强力霉素治疗由沙眼衣原体和解脲支原体感染引起的非淋菌性宫颈炎的疗效。方法:将门诊确诊的非淋菌性宫颈炎患者随机分为治疗组48例,应用克拉霉素口服联合阴道局部应用强力霉素;对照组用克拉霉素口服。疗程14d,停药1周后随诊观察疗效。结果:治疗组的治愈率为93.75%,总有效率为97.92%;对照组的治愈率为78.26%,总有效率为84.78%;两组的治愈率和总有效率有显著性差异(P均<0.05)。结论:口服克拉霉素联合阴道局部用强力霉素治疗非淋菌性宫颈炎疗效较好,且患者依从性好。     

黏液脓性宫颈炎患者的感染因素及病原体分布

目的探索分析黏液脓性宫颈炎患者的感染因素以及病原体分布情况,为黏液脓性宫颈炎患者的临床抗菌治疗提供有利的证据。方法选取2018年1月至2019年6月于本院妇科门诊的360例宫颈筛查患者为研究对象,分别收集360例宫颈筛查患者的宫颈分泌物,根据分泌物的检测结果分为黏液脓性宫颈炎组(n=180)和宫颈无黏液脓性分泌物组(n=180),分析比较两组患者支原体感染检出阳性率及各种支原体检出率。结果与宫颈无黏液脓性分泌物组患者支原体感染检出阳性率比较,黏液脓性宫颈炎组患者支原体阳性检出率明显较高,黏液脓性宫颈炎组病原体检出率为91.11%,其中沙眼衣原体的检出率(45.12%)明显高于其他病原体检出率,两组比较差异具有统计学意义(P<0.05)。结论黏液脓性宫颈炎患者体内存在多种病原体感染,加强对宫颈炎分泌物中病原体的检出,能有效提高抗菌药物选择的正确性,提升黏液脓性宫颈炎患者的治愈率。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.