131Ⅰ－McAbJH_（107）在载人肝癌BEL－7402裸鼠体内的放射免疫显像分析

用氯胺Ｔ碘标技术标记抗人肝癌单克隆抗体ＪＨ１０７（ＭｃＡｂＪＨ１０７），观测其在载人肝癌ＢＥＬ７４０２裸鼠模型上的分布显像情况，氯胺Ｔ碘标法标记率５０％。每鼠腹腔注射２００Ｃｉ１３１Ⅰ－ＭｃＡｂＪＨ１０７，放射性物质第２４ｈ开始在肿瘤部位浓聚、逐步加强，９６ｈ达高峰，肿瘤组织较清楚显像并维持至１４８ｈ；同时周围组织放射性本底逐步减弱、消失。对照组１３１Ⅰ－ＮＩｇＧ呈全身均匀分布。无明显放射性物质浓聚及清楚显像。在４８ｈ和９６ｈ，１３１Ⅰ－ＭｃＡｂＪＨ１０７在１２种正常组织（脑除外）的Ｔ／ＮＴ均值分别为３．３８和６．２６，而１３１Ⅰ－ＮＩｇＧ的Ｔ／ＮＴ均值均低于１．０。     

^131I标记花生凝集素的制备及其受体亲合活性

本文报道＾１３１碘－花生凝集素（＾１３１Ｉ－ＰＮＡ）的制备方法。采用Ｉｏｄｏｇｅｎ法进行放射性碘化标记，标记率为６４％￣７０％，平均标记率６７．９％；放化纯度为８７％￣９８％。经柱层析后得到纯品，通过纸层析鉴定为单一放射性峰，用自身取代法测定其比放射性为２０９￣３１４ｋｂｑ／μｇ。它与ＰＮＡ受体结合，具有亲合力高及特异性强的生物活性。     

抗人AFP－R－LCA McAb在裸鼠人肝癌模型中放免显像及治疗

放射性核素~（１３１）Ｉ标记的抗人小扁豆凝集素结合型甲胎蛋白异质体（ＡＦＰ－Ｒ－ＬＣＡ）ＭｃＡｂ，注入荷瘤裸鼠腹腔后，能在裸鼠人肝癌模型肿瘤区积聚，其放射性核素浓度是裸鼠肝脏的５．２倍；而~（１３１）Ｉ标记的正常小鼠ＩｇＧ（ｍＩｇＧ）及游离~（１３１）Ｉ却无肿瘤区积聚，且在荷瘤裸鼠体内呈均匀分布。~（１３１）Ｉ－ＡＦＰ－Ｒ－ＬＣＡＭｃＡｂ组γ照像均显示出裸鼠人肝癌的阳性定位。用~（１３１）Ｉ－ＡＦＰ－Ｒ－ＬＣＡＭｃＡｂ治疗裸鼠人肝癌，肿瘤生长受到明显抑制（８５．０％），优于~（１３１）Ｉ－ｍＩｇＧ及游离~（１３１）Ｉ（４６．０％、２０；１％，Ｐ＜０．０５）．结果表明：ＡＦＰ－Ｒ－ＬＣＡＭｃＡｂ对人肝癌细胞有较强的亲和力和特异性，有希望成为肝癌导向研究的理想载体。     

肝癌导向治疗中三种^131Ⅰ标记抗体的药代动力学观察

作者对＾１３１Ⅰ标记铁蛋白多克隆抗体，抗人肝癌单克隆抗体和乙型肝炎病毒Ｘ蛋白单克隆抗体的药代动力学进行研究。结果：（１）裸鼠人肝癌模型＾１３１Ⅰ－ＦｔＡｂ分别经静脉，腹腔途径给药，其药代动力学模式有很大差异，相同剂量的ＦｔＡｂ与ＨＢｘＭｃＡｂ经ｉｐ给药Ｔｍａｘ分别为８．１ｈ８．４ｈ，Ｔ１／２分别为４０．５ｈ和４２．２ｈ；不同剂量Ｔ的＾１３１Ⅰ－ＨＢｘＭｃＡｂ，低剂量组与高剂量组Ｔｍａｘ分别为１４．     

^131I—CAE单抗在荷瘤裸鼠体内分布及治疗结肠癌肝转移？…

目的 探讨＾１３１Ｉ标记抗ＣＥＡ单抗在裸鼠人结肠癌肝转移模型的体内分布及其抑制肝转移的作用。方法 在建立裸鼠人结肠腺癌肝转移模型的基础上,用＾１３１Ｉ标记抗ＣＥＡ单抗ＣＬＯ＾－１,注射荷瘤裸鼠,观察＾１３１Ｉ标记抗ＣＥＡ单抗在荷瘤裸鼠体内分布及抑制肝转移的作用。结果 体外标记抗体特异性结合率为５９．９％。在注射＾１３１Ｉ标记ＣＬＯ－１后２４～９６ｈ肝转移灶内特异性放射性浓聚,９６ｈ瘤／肝比值为４．     

EHF病毒在人肾细胞融合中的结构蛋白分子定位分析和人胚？…

用辣根过氧化物酶标记ＭｃＡｂＦ（ａｂ′）２，ＭｃＡｂＬＶ４８Ａ，ＭｃＡｂ３Ｄ３８和ＭｃＡｂＡ３５，分别对２７例ＥＨＦ患者尿液中融合细胞（ＦＣ）进行免疫酶染色，结果ＭｃＡｂＦ（ａｂ′）２检出率为２７／２７（１００％）；ＭｃＡｂＬＶ４８Ａ为１９／２７（７０．３７％），ＭｃＡｂ３Ｄ８为２０／２７（７４．０７％）；ＭｃＡｂＡ３５为２／２７（７．４１％），人胚肾细胞感染ＥＨＦ病毒后，在偏酸性（ｐＨ６．０）条     

Iodogen法^125Ⅰ标记重组人粒细胞集落刺激因子的研究

采用Iodogen(四氯二苯基苷脲)法进行^125Ⅰ标记重组人粒细胞集落刺激因子(rhG-CSF)，标记物用SephadexG-25柱凝胶过滤法分离、纯化，用薄层层析法和三氯醋酸法鉴定标记物的放射化学纯度、游离碘率及碘标记率和标记物的稳定性。结果标记物的放化纯度为97．8％，碘标记率为78．4％，比活度为86．34TBq／mmol。用Iodogen法^125Ⅰ标记rhG-CSF，方法简便、标记率高、稳定性好。     

^131碘标记单克隆抗体对移植人肺腺癌裸鼠的放射免疫治疗

肺癌在近年来发病率和死亡率都有明显上升的趋势，尽管各种治疗方法有了明显改进，但５年生存率并无多大改善［１］。作者应用放射免疫治疗的方法，对肺腺癌进行了实验研究，报道如下。一、材料和方法１．抗体的提纯及鉴定：含单抗ＳＣ１３Ａ的鼠腹水离心取上清，经ＳＰＡ－ＳｅｐｈａｒｏｓｅＣＬ－４８提纯，用ＳＤＳ－ＰＡＧＥ鉴定纯度＞９５％。小鼠正常ＩｇＧ自制。２．抗体标记及活性测定：按氯胺Ｔ法标记１３１Ｉ。１３１Ｉ－ＳＣ１３Ａ和１３１Ｉ－ＮＭＩｇＧ放化纯度分别为９２％和９４％，标记率分别８８％和８６％，比放分别为２…     

~(131)I标记花生凝集素的制备及其受体亲合活性

本文报道１３１碘－花生凝集素（１３１Ｉ－ＰＮＡ）的制备方法。采用Ｉｏｄｏｇｅｎ法进行放射性碘化标记，标记率为６４％～７１％，平均标记率６７．９％；放化纯度为８７％～９８％。经柱层析后得到纯品，通过纸层析鉴定为单一放射性峰，用自身取代法测定其比放射性为２０９～３１４ｋｂｑ／μｇ。它与ＰＮＡ受体结合，具有亲合力高及特异性强的生物活性。     

普服碘盐后正常人及几种甲状腺疾病患者甲状腺摄^131 …

目的：为了建立普服碘盐后新的甲状腺摄＾１３１碘率的正常实验比值。方法：测定７０名正常人及２２４例甲状腺疾病患者的摄＾１３１碘率。受检者均空腹口服＾１３１碘７４－１８５ｋｂｑ，用ＨＦ４６３Ａ型甲状腺功能仪测定３ｈ、２４ｈ摄＾１３１碘率。结果：普服碘盐后，正常组摄＾１３１碘率较８０年代明显降低，有非常显著差异，１４８例甲状腺机能亢进患者的摄＾１３１碘率与 ８０年代甲亢患者比无明显差异，其中９７例例未经     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.