自体表皮细胞-纤维蛋白膜创面移植治疗大鼠烧伤

目的：观察自体表皮细胞-纤维蛋白膜移植到大鼠烧伤创面治疗皮肤缺损的效果。方法：健康Wistar大鼠20只,随机分成烧伤皮肤缺损造模组和自体表皮细胞-纤维蛋白膜移植治疗组,治疗后计算表皮细胞在纤维蛋白膜上最佳接种密度,观察移植后的各组创面愈合情况、创面伤口的收缩比例等。结果：在纤维蛋白膜上接种表皮细胞的最佳密度为5×10^4／cm2,烧伤皮肤缺损造模组创面完全愈合时间平均22．3d,自体表皮细胞-纤维蛋白膜移植治疗组为18．1d,造模组创面收缩率为（70±5）％,移植组为（20±5）％（均P〈0．05）。结论：自体表皮细胞-纤维蛋白膜可用于覆盖大面积烧伤造成的皮肤缺损,预防创面伤口瘢痕化的形成,减轻创面收缩率,加速皮肤缺损创面的愈合速度。     

康复新联合赛肤润治疗大鼠急性放射性皮肤损伤效果观察

目的 观察康复新联合赛肤润治疗大鼠急性放射性皮肤损伤的疗效。 方法 取40只SD大鼠，用放射性核素32P敷贴固定于大鼠背部皮肤以制备急性放射性皮肤损伤模型。建模后，将大鼠随机分为模型组、康复新组、赛肤润组、康复新联合赛肤润组（联合组），各10只。模型组用生理盐水擦拭皮损处；康复新组用康复新涂抹皮损处；赛肤润组用赛肤润喷涂皮损处；联合组先涂抹康复新液，待干后喷涂赛肤润，2次/d。各组均在8：00及16:00固定时间涂抹药液。观察创面局部变化及愈合时间。治疗第2周、4周、8周时取皮损创面组织进行苏木精-伊红染色和Masson染色。 结果 与模型组比较，康复新组、赛肤润组、联合组的愈合时间显著缩短（均P＜0.05）；联合组的愈合时间显著短于康复新组及赛肤润组（均P＜0.05）；组织病理学检测结果显示，联合组创面组织炎性细胞浸润、水肿程度显著低于其他组，新生毛细血管数量及胶原纤维含量显著高于其他组，胶原纤维排列更整齐，表皮结构更加完整。与模型组比较，治疗第2周、4周、8周时康复新组、赛肤润组、联合组的胶原纤维含量较高（均P＜0.05）；与康复新组、赛肤润组相比，联合组的胶原纤维含量较高（均P＜0.05）。 结论 康复新联合赛肤润能够减轻放射性皮肤损伤创面早期炎症反应，促进创面血管新生与胶原纤维形成，缩短创面愈合时间，加快创面修复。     

细胞免疫与烧伤创面愈合的实验研究

为探讨免疫低下对烧伤创面愈合的影响，设计了用低剂量钴源照射的方法建立的免疫抑制模型，并在该模型的基础上观察了免疫抑制大鼠Ⅱ度烧伤创面的情况，结果表明：（１）大鼠接受２Ｇｙ＾６０Ｃｏ照射后２０天内，Ｔｈ／Ｔｓ比值和Ｔ细胞肿瘤花环率持续下降，照射皮肤组织ＤＮＡ含量及细胞周期均无明显改变；（２）免疫抑制大鼠胶原修复能力下降，创面愈合百分率低下正常大鼠，由此证实了免疫功能低下对创面愈合的抑制作用。     

大鼠深Ⅱ度烫伤创面愈合中血管形成与血流变化特点

目的：观察大鼠深Ⅱ度烫伤模型创面愈合中血管形成与血流改变的特点。方法：Wistar大鼠48只,随机分为A、B两组,A组（n=42）：为组织病理观察组,根据实验设计又分伤后即刻、1天、3天、7天、14天及21天及正常对照组,每组6只;B组（n=6）：为血流量检测组。在实验大鼠背部制作深Ⅱ度烫伤模型,A组大鼠在各时间点活杀取材,进行病理和免疫组化检测;B组大鼠连续检测背部同一部位正常皮肤及伤后各时间点的创面血流量。结果：烫伤大鼠伤后3～7天,肉芽组织内出现单个的不成腔的血管内皮细胞,数目随肉芽组织的增多而逐渐增加,后逐渐形成管腔;伤后14天,肉芽组织内有腔毛细血管进一步增多,已出现结构较完整的成熟小血管;伤后21天血管形态和数量已接近正常皮肤。深Ⅱ度烫伤大鼠伤后即刻,皮肤血流量锐减为正常皮肤血流量的51％;伤后3天时,创面血流量仅迭正常血流量的68％;至伤后14天,创面血流量升到正常值的88％;14天后逐渐正常。结论：在皮肤深Ⅱ度烫伤创面愈合过程中,随着肉芽组织内内皮细胞数量和结构的变化,创面的血流量不断增加,前者的变化是后者变化的基础。     

大鼠皮肤碱烧伤早期病理表现及其组织学变化

目的:复制实验动物皮肤碱烧伤模型,观察组织形态病理学变化,以研究化学烧伤的临床治疗问题。方法:Wistar大鼠20只,取NaOH溶液2mol/L、2.5mol/L、5mol/L等三组,分别均匀涂抹于大鼠背部,观察时间为30s、45s、60s、75s,致大鼠皮肤形成碱烧伤后15mm取材,常规切片组织学观察。结果:NaOH溶液2mol/L(60s)、2.5mol/L(45s)、5mol/L(30s)可致大鼠皮肤Ⅱ°伤;2mol/L(75s)、2.5molL(60S)、5mol/L(45s)形成Ⅲ°伤,其烧伤深度与NaOH浓度和作用时间成正比。结论:NaOH不同浓度和作用时间对大鼠皮肤碱烧伤为渐进性损伤,与潜掘状损伤似乎不同。另外不同浓度的NaOH溶液在相同时间对大鼠皮肤组织渗透及同一浓度NaOH溶液在不同时间对皮肤的损伤病理学变化均有差异。     

组织工程微粒真皮的构建及其动物实验研究

目的：将人成纤维细胞和York猪脱细胞真皮基质（ADM）微粒复合构建的？组织工程微粒真皮应用于SD大鼠损伤模型，观察其修复组织创伤能力。方法：用PKH26标记的人成纤维细胞，与猪ADM微粒复合构建微粒真皮，将其注射到SD大鼠皮下，用荧光显微镜和HE观察ADM微粒真皮对皮下组织的修复；将54只SD大鼠随机分成3组，分别移植自体全厚皮肤、ADM微粒和ADM微粒皮真皮。大体、荧光显微镜和HE观察伤口愈合情况、计算创面愈合时间和收缩率。结果：皮下注射ADM微粒真皮2周时可见单核细胞、成纤维细胞和血管长入，以后单核细胞减少，荧光观察可见红色荧光，8周时注射的微粒真皮基本被自身组织改建利用、体积稳定，胶原粗大整齐；ADM微粒真皮移植能够使创面愈合时间、创面收缩率与自体全厚皮肤移植相比无统计学意义（P〉0．05），与ADM微粒移植相比差异显著（P〈0．01）。结论：皮下注射ADM微粒真皮可参与皮下组织改建，修补皮下组织缺损；ADM微粒真皮可促进SD大鼠损伤模型的愈合和瘢痕挛缩。     

大鼠感染性创面肉芽组织胶原代谢的改变

目的：观察大肠杆菌诱导的大鼠感染性创面修复过程中创面肉芽组织中蛋白含量、羟脯氨酸含量及胶原合成的变化。方法：Wistar大鼠48只,随机分为感染组和对照组,切取大鼠背部2.0cm×2.0cm大小皮肤、皮下组织,形成开放性创面,感染组采用含9×108CFU/mL大肠杆菌1mL培养液涂擦创面,术后第3d、第7d、第15d和第22d分别处死感染组和对照组各6只,观察创面愈合时间和未愈合面积,测定创面肉芽组织中蛋白含量、羟脯氨酸含量和创面I、Ⅲ型胶原含量及比值。结果：大肠杆菌诱导的大鼠感染性创面愈合时间为（21.8±0.2）d,显著长于对照组（19.5±0.75）d（P〈0.01）。感染组在各时间点创面未愈合面积明显大于对照组（P〈0.05）。感染组在不同时间点的创面肉芽组织中蛋白、羟脯氨酸含量和创面I、Ⅲ型胶原含量及其比值明显低于对照组（P〈0.01）。结论：大肠杆菌可能通过影响创面肉芽组织中蛋白含量、羟脯氨酸含量及胶原合成,从而导致感染创面愈合延缓。     

大鼠深Ⅱ度烧伤创面保留变性真皮并覆盖自体皮疗效观察

目的为探讨自体皮覆盖变性真皮修复深Ⅱ度烧伤创面的可行性提供实验依据。方法在大鼠背部造成直径3.5cm深Ⅱ度烧伤创面。伤后2—5d行创面浅层削痂保留变性真皮,同时在局部移植大张自体断层皮片。移植前和移植后不同时相点分别切取植皮区全层皮肤,光镜下观察其形态学及胶原纤维变化,并检测其生物力学改变。取移植大鼠同体背部正常皮肤作为对照指标。结果(1)保留的变性真皮为玻璃样变性。(2)大鼠自体皮移植术后7d,皮片与创面融合无法分离,光镜下可见真皮乳头及网状层。术后21d移植部位皮肤厚度、结构、形态与正常组织相似,有萎缩毛囊,胶原纤维条索密度逐渐增大接近融合。(3)鼠皮抗拉强度、最大应变值在术后逐渐增大,至60d时接近正常。结论将自体皮覆盖于变性真皮上用以修复深Ⅱ度烧伤创面,变性真皮能够逐渐复苏,使其结构、形态接近正常。     

硝普钠和磺胺嘧啶银联合应用对大鼠深Ⅱ度烧伤创面愈合的影响

目的：研究硝普钠、磺胺嘧啶银及二者合用对深Ⅱ度烧伤皮肤创面愈合的影响。方法：100只WiStar大鼠背部深Ⅱ度烧伤创面,随机分成0．9％氯化钠注射液组、1％磺胺嘧啶银霜组、lmmol／L硝普钠组和1％磺胺嘧啶银霜＋lmmol／L硝普钠组。每组25只大鼠。动态观察烧伤后不同时间点创面细胞增殖周期、羟脯氨酸含量及创面组织愈合情况,计算创面愈合率。结果：伤后随时间推移磺胺嘧啶银霜＋硝普钠组创面愈合率高于其他三组;伤后第10天,磺胺嘧啶银霜＋硝普钠组创面羟脯氨酸含量、细胞S期百分比达到峰值,明显高于0．9％氯化钠注射液（P〈O．01）,磺胺嘧啶银霜组、硝普钠组之间比较无显著差异（P〉0．05）。结论：磺胺嘧啶银霜和硝普钠合用可有效促进大鼠深Ⅱ度烧伤创面愈合。     

复合骨髓间充质干细胞的组织工程皮肤修复烧伤创面的实验研究

目的：探讨用复合骨髓间充质干细胞（MSCs）的组织工程皮肤修复烧伤创面的可行性。方法：利用自行研制的烫伤仪制备烫伤创面，以MSCs为种子细胞构建组织工程皮肤修复烫伤创面，观察愈合时间和收缩率等。结果：移植2周后实验组创面基本愈合，表皮角质化，真皮层毛细血管增生，6周后创面收缩到原始面积的61％。结论：含有MSCs的组织工程皮肤移植后促进了烧伤创面的愈合，获得了较理想的愈合效果，为临床治疗提供了新的思路。     

Polyunsaturated phosphatidylcholine protects against wound contraction in experimental skin burn injury.

Polyunsaturated phosphatidylcholine (PP) is a soybean lecithin, whose oral supplementation prevents fibrosis and strictures in animal models. The purpose of the present study was to investigate the effect of PP on wound contraction using a model of skin burn injury. Thirty male Sprague-Dawley rats were randomly divided into three groups: control (C), just burn (JB), and burn treated with PP (BPP). Burns were induced on the dorsum of the rats by a metal plate with a 4-cm(2) contact surface. Ten percent emulsion of PP was given orally for 21 days in BPP group. The effects of PP on burns were evaluated histologically on day 21, and wound contraction was measured by planimetry weekly. Microscopic evaluation included the amount and density of myofibroblasts and collgen type III, the existence of mature collagen fibers in the regenerated dermis, and thickness measurements of the regenerated tissue. There was a significant decrease in mean wound size index in group JB when compared to BPP on days 14 and 21 (p <.05 and p <.01, respectively). After 3 weeks, the granulation tissue was more cell dense, containing high numbers of myofibroblasts in group JB when compared to BPP (p <.05). The wounds had more mature collagen bundles, but less collagen type III in group BPP when compared to JB. Thus, polyunsaturated phospatidylcholine protected against wound contraction and modulated wound healing in the rat model of skin burn injury.     

烫伤大鼠不同深度创面组织中表皮干细胞分布的初步研究

目的初步观察烫伤大鼠不同深度创面组织中表皮干细胞的分布情况。方法将32只SD大鼠分别造成Ⅰ、浅Ⅱ、深Ⅱ和Ⅲ度烫伤，伤后24h取创面组织标本，制作切片。采用细菌蛋白一生物素标记法(LSAB)进行免疫组织化学染色，以α2、β1整合素及角蛋白10(K10)作为一抗，观察不同创面组织中表皮干细胞的表达和分布情况。结果Ⅰ度烧伤创面组织中，K10阳性细胞分布于表皮的棘细胞层、颗粒层、透明层，α2、β1整合素阳性细胞分布于表皮基底层，数量多。浅Ⅱ度创面中，α2、β1整合素阳性细胞位于残留的基底层和皮肤附属器(主要是毛囊)中，数量较少。深Ⅱ度创面中，α2、β1整合素阳性细胞仅存在于真皮深层健存的皮肤附属器中，数量很少。Ⅲ度创面中，罕见α2、β1整合素阳性细胞。结论表皮干细胞在烧伤创面中的分布与烧伤深度有关，残存的表皮干细胞可能是创伤修复过程中再上皮化的细胞来源。     

一种大鼠蒸气烫伤模型的建立

目的　建立一种可控深度及面积的大鼠烫伤模型。　方法　用高压蒸气消毒锅及自制烫伤支架制作高压蒸气烫伤装置 ,用压力为 0 .12MPa(1MPa=75 0 0mmHg)、直径 2 .6cm的致伤孔上分别在大鼠背部烫 3、4、5、6、7、8、9、10s,每时相点 5个创面。伤后 2 4h取标本行组织学观察 ,用Photoshop软件测量烫伤深度 ,并观察有无毛囊、汗腺附件受损、烫伤区被毛生长及创面愈合情况。　结果　烫伤深度与烫伤时间的变化呈正相关 (r =0.99)。浅Ⅱ、深Ⅱ、Ⅲ度烫伤模型的致伤时间分别为 3、5、7s。烫伤 7～ 10s创面深度虽逐渐加重 ,愈合时间却相近。　结论　该模型可以控制烫伤深度、面积 ,烧伤深度划分准确且操作简便 ,是研究创伤修复机制及评价创面用药的较好模型。     

大鼠烫伤创面感染模型的研制

目的 拟建立稳定的大鼠烫伤创面感染模型,以便于进行相关防治研究.方法 (1)取50只SD大鼠,使用恒温恒压烫伤仪,以底面积4.5 cm2、质量0.5kg的80℃圆柱形烫头垂直接触大鼠脊柱左右两侧皮肤,致伤4、6、8、10、12 s(每种致伤时间10只大鼠,左右侧烫伤时间相同)制作烫伤模型.伤后24 h,观察创面大体情况,记录左侧创面愈合时间,取右侧创面组织行组织学观察,根据结果筛选浅Ⅱ度、深Ⅱ度创面致伤时间.(2)另取36只SD大鼠,按随机数字表法分为浅Ⅱ度组、深Ⅱ度组,每组18只,按照前述方法与选定的致伤时间分别制成浅Ⅱ度、深Ⅱ度烫伤创面.伤后即刻在2组大鼠一侧创面分别接种0.1 mL含1×109、1×107、1×105CFU铜绿假单胞菌标准菌株ATCC 27853的菌液(每种菌量6只大鼠),在另一侧创面涂抹等体积生理盐水作为对照.接种细菌后24 h HE染色观察创面炎症反应情况;接种细菌后1、2、3、5、7、14 d进行革兰染色及生化反应鉴定菌种,检测并计算痂下细菌含量;记录2组大鼠创面愈合时间.对数据行t检验.结果 (1)根据大鼠创面愈合时间及组织学检查结果,筛选出烫伤6 s和8 s分别为浅Ⅱ度和深Ⅱ度创面的致伤时间.(2)浅Ⅱ度组大鼠仅接种1×109CFU细菌的创面有少许炎性细胞浸润;深Ⅱ度组接种1×109、1×107CFU细菌创面均有炎性细胞浸润,前者浸润更明显.(3)创面细菌鉴定结果为铜绿假单胞菌.浅Ⅱ度组创面接种各种菌量后14 d内,痂下细菌含量绝大多数低于1×105CFU/g;深Ⅱ度组创面接种1×109 CFU细菌后14 d内,痂下细菌含量均高于1×105CFU/g并呈持续上升趋势.(4)浅Ⅱ度组接种1×109、1×107、1×105 CFU细菌的创面与生理盐水对照创面愈合时间相近(t值分别为1.26、0.29、1.07,P值均大于0.05);深Ⅱ度组接种1×109CFU细菌创面愈合时间[(22.5±1.0)d]较生理盐水对照创面[(19.4±1.6)d]明显延长(t=2.73,P＜0.05).结论 大鼠深Ⅱ度烫伤创面接种1×109 CFU铜绿假单胞菌标准菌株ATCC 27853,可作为感染创面防治研究的实验模型,该模型稳定、重复性较高.

Abstract：

Objective To reproduce a reliable rat model of burn with infection for the study of prevention and treatment of infected wound. Methods ( 1 ) Electrical burn producing apparatus equipped with constant temperature (80 ℃ ) and pressure (0.5 kg) was used to reproduce burn injury (with area of 4.5 cm2 ) on both sides of the back in 50 SD rats for different duration (4, 6, 8, 10, 12 s) , with 10 rats for each burn duration. On post burn day (PBD) 1, gross condition of wounds was observed with naked eyes.Wounds on the left side were used to observe healing time. The wounds on the right side were used for histological observation to determine the depth of injury, and they were classified into superficial and deep partialthickness injury. (2) Another 36 SD rats were divided into A (inflicted with superficial partial-thickness burn, n = 18) and B (inflicted with deep partial-thickness burn, n = 18) groups according to the random number table. Rats in both groups were treated in accordance with method of preliminary experiment. Immediately after burn, 0. 1 mL of liquid containing 1 × 109, 1 × 107, 1 × 105 CFU Pseudomonas aeruginosa (PA) ATCC 27853 was respectively inoculated to the wounds on one side (with 6 rats for each amount) ,while the wounds on the other side were treated with the same volume of normal saline as control. Inflammatory reaction of wounds was examined with HE staining on post inoculation day (PID) 1. On PID 1, 2, 3,5, 7, and 14, the number of subeschar bacteria was respectively counted and the bacteria were identified with Gram stain and biochemical reaction. Wound healing time was recorded. Data were processed with t test. Results (1) Burn for 6, 8 s was respectively identified as injury time resulting in superficial or deep partial-thickness injury according to histological observation and wound healing time. (2) Obvious inflammatory cell infiltration was observed in the wounds in B group which were inoculated with 1 × 107 , 1 ×109 CFU PA, and the infiltration was less marked in A group with inoculation of 1 × 109 CFU PA. (3) The bacteria isolated from wounds of A and B groups was identified as PA. The subeschar bacteria count within PID 14 in A group, in which different amount of PA was inoculated, was mostly less than 1 × 105 CFU/g of tissue, while that in B group in which 1 × 109 CFU PA was inoculated was more than 1 × 105 CFU/g of tissue. (4) There was no obvious difference in wound healing time between wounds inoculated with different amount of PA and wounds treated with normal saline in A group ( with t value respectively 1.26, 0. 29, 1.07,P values all above 0.05 ). Wound healing time of wounds in B group, in which 1 × 109 CFU PA was inoculated, was longer as compared with that treated with normal saline [(22.5 + 1.0) d vs. ( 19.4 + 1.6) d, t =2.73, P ＜0. 05]. Conclusions In rat, deep partial-thickness burn wound inoculated with 1 × 109 CFU PA ATCC 27853 is a reliable model with high reproducibility for the study of infection of burn wound.     

Tissue‐Engineered Skin Containing Mesenchymal Stem Cells Improves Burn Wounds

There is increasing evidence showing that adult stem cells are useful for tissue regeneration. Bone marrow mesenchymal stem cells (MSCs) are self‐renewing and are potent in differentiating into multiple cells and tissues. To investigate the practicability of repairing burn wounds with tissue‐engineered (TE) skin combined with bone MSCs, we established a burn wound model in the porcine skin. With a controlling temperature and time of the burning device to obtain different degrees of burn wounds, a deep dermal partial thickness burn was introduced to the porcine skin using a heated‐brass contact injury at 100°C for 20 s. Collagen‐GAG scaffolds were utilized as the matrix; MSCs separated from pigs were seeded on them to form the skin equivalent. When grafted to the burn wounds, the TE skin containing MSCs showed better healing and keratinization, less wound contraction, and more vascularization. Grafts proliferated well and contributed to the neo‐tissues. These data suggest that TE skin containing MSCs in a burn defect can accelerate wound healing and receive satisfactory effects.     

巴曲酶对深Ⅱ度烫伤创面微循环血流变化及愈合的影响

目的　探讨巴曲酶对大鼠烫伤早期创面淤滞带微循环血流变化及愈合过程的影响。　方法　Wistar雄性大鼠 2 0只 ,随机分为烫伤对照组及巴曲酶治疗组 ,在各组动物背部均造成 4cm× 4cm大小深Ⅱ度烫伤。分别于伤前、伤后 0 .5、2、4、6、12、2 4、72h测定创面局部皮肤血流量 ;于伤后立即、伤后 14、18d测定烫伤面积及残留创面面积 ;于伤后 30d将大鼠处死取材行组织学观察及毛囊计数。　结果　大鼠伤后 2 72h皮肤血流量进行性减少 ;与对照组相比 ,给予巴曲酶后皮肤血流量得到明显改善 ,创面愈合速度明显加快 ,愈合后皮肤附件明显增多。　结论　巴曲酶能逆转烧伤后创面淤滞带循环 ,加快创面愈合速度和提高皮肤愈合质量     

烧伤后24 h内削痂防治深Ⅱ度创面进行性加深的组织学观察

目的　探讨烧伤后 2 4h内行削痂手术 ,对深Ⅱ度烧伤创面进行性加深的防治作用。方法　选择 12例深Ⅱ度烧伤患者 ,在伤后 2 4h内进行创面削痂手术。分别从手术前、手术后 (伤后 5～7d)和未手术创面 (伤后 5～ 7d)获取标本。采用HE染色、Masson′s染色和免疫组化技术标记Vi mentin抗原阳性细胞的方法 ,对创面组织标本进行组织学观察。　结果　随病程演进 ,未手术创面炎性反应程度明显加重 ,组织坏死范围扩大 ,原来残存的皮肤附件因炎症加重而消失 ;Masson′s染色棕红色范围扩大 ,亮绿色范围缩小 ;Vimentin抗原阳性细胞数量明显减少。伤后 2 4h内削痂创面局部炎症反应较未削痂创面明显减轻 ,可见新鲜肉芽形成和部分上皮修复 ,组织坏死范围未见进一步扩大 ,与未手术区比较差异有显著性意义 (P <0 .0 5 ) ;Masson′s染色亮绿色范围无明显缩小 ;Vimentin抗原阳性细胞数量明显多于未手术区 (P <0 .0 5 )。　结论　烧伤后 2 4h内削痂 ,能适时去除创面坏死组织、阻断组织变质性损害的加剧 ,从而有效改善深Ⅱ度创面的进行性加深现象 ,对创面愈合具有促进作用。     

Porcine dermal collagen as a wound dressing for skin donor sites and deep partial skin thickness burns

Collagen was extracted by pepsin digestion from porcine skin, and collagen membrane was prepared by salt precipitation. The porcine collagen membrane was evaluated as a burn wound dressing in deep partial skin thickness burn wounds in rats. Burn wounds, 4 × 4 cm, were inflicted by exposure of skin to 75°C for 15 s followed by de-epithelialization. Wound healing was assessed by planimetry of epithelialization on day 10 after injury. Open wounds exhibited 24 per cent of wound area re-epithelialized. Collagen membrane dressing significantly improved the healing to 69 per cent of wound area (P < 0.0001). In a completely separate experiment, the porcine collagen membrane was applied as a wound dressing to the donor sites of burn patients, and its effect on wound healing was compared with that of a petroleum jelly gauze dressing. The donor sites covered with petroleum jelly gauze had re-epithelialized by an average of 14.5 days (ranging from 13 to 16 days) after wounding. The wounds dressed with collagen membrane demonstrated a significant increase in the healing rate. Complete re-epithelialization was observed by 10.3 days (ranging from 10 to 12 days) after wounding (P < 0.0001).     

Stromal vascular fraction improves deep partial thickness burn wound healing

ObjectiveThe practice of early burn wound excision and wound closure by immediate autologous skin or skin substitutes is the preferred treatment in extensive deep partial and full-thickness burns. To date there is no proven definite medical treatment to decrease burn wound size and accelerate burn wound healing in modern clinical practice. Stromal vascular fraction is an autologous mixture that has multiple proven beneficial effects on different kinds of wounds. In our study, we investigated the effects of stromal vascular fraction on deep partial-thickness burn wound healing.MethodsIn this study, 20 Wistar albino rats were used. Inguinal adipose tissue of the rats was surgically removed and stromal vascular fraction was isolated. Thereafter, deep second-degree burns were performed on the back of the rats by hot water. The rats were divided into two groups in a randomized fashion. The therapy group received stromal vascular fraction, whereas the control group received only physiologic serum by intradermal injection. Assessment of the burn wound healing between the groups was carried out by histopathologic and immuno-histochemical data.ResultsStromal vascular fraction increased vascular endothelial growth factor, proliferating cell nuclear antigen index, and reduced inflammation of the burn wound. Furthermore, vascularization and fibroblastic activity were achieved earlier and observed to be at higher levels in the stromal vascular fraction group.ConclusionsStromal vascular fraction improves burn wound healing by increasing cell proliferation and vascularization, reducing inflammation, and increasing fibroblastic activity.