脱细胞真皮基质和聚丙烯补片修补大鼠腹壁疝的实验研究

Objective To study the value of porcine acellular dermal matrix(ADM) made by complex maceration and Marlex mesh as ventral hernia-repairing material. Methods ADM was made from the full thick skin of the back of swines by complex maceration. The male SD rats were randomly divided into three groups including ventral hernia group(n =7), ADM group(the ventral hernia repaired by ADM, n =28) and Marlex mesh group (the ventral hernia repaired by Marlex mesh group, n = 10). The occurrence of ventral hernia was observed on postoperative 1 week. Six rats were respectively selected from the Marlex group and ADM group and tension resistance at 5weeks after surgery was observed. Results The occurrence of ventral hernia in ADM group and Marlex mesh group was significantly lower than that of ventral hernia group(0.04%, 0.0%, 100.0%, P ＜ 0. 01 ) in postoperative 1week, but there was no difference between ADM group and Marlex mesh group ( P ＞ 0.05 ). Marlex had a significantly higher breaking strength than ADM[(417 ±44)N vs( 111 ±27)N,P ＜0.01]. But 5 weeks after surgery, the tension resistance of ADM-fascial interface was higher than that of the Marlex-fascial [( 103 ± 27 ) N vs(71 ± 19 ) N,P ＜ 0. 05]. Conclusion As a material of repairing ventral hernia, ADM prepared by the complex maceration may be superior to Marlex mesh.     

脱细胞真皮基质和聚丙烯补片修补大鼠腹壁疝的实验研究

目的 探讨复合消蚀法制备的脱细胞真皮基质(ADM)和聚丙烯补片(Marlex网)修补大鼠腹壁疝的应用价值.方法 用全厚猪皮制备ADM,选用SD雄性大鼠45只,完全随机分为腹壁疝组7只、Marlex网组10只和ADM组28只,观察3组术后1周时有无腹壁疝的发生,术后第5周Marlex网组和ADM组随机选取6只用于抗张力试验.结果 术后1周时,ADM组与Marlex网组腹壁疝发生率均明显低于腹壁疝组(分别为0.04%、0.00%、100.00%,P＜0.01),但ADM组与Marlex网组差异无统计学意义(P＞0.05).将单独ADM片与Marlex网行抗张力试验,Marlex网的抗张力远高于ADM[(417±44)N比(111±27)N,P＜0.01].但植入体内5周后,ADM-筋膜组织的抗张力高于Marlex-筋膜组织[(103±27)N比(71±19)N,P＜0.05].结论 复合消蚀法制备的ADM可能比聚丙烯补片更适合作为疝修补材料.

Abstract：

Objective To study the value of porcine acellular dermal matrix(ADM) made by complex maceration and Marlex mesh as ventral hernia-repairing material. Methods ADM was made from the full thick skin of the back of swines by complex maceration. The male SD rats were randomly divided into three groups including ventral hernia group(n =7), ADM group(the ventral hernia repaired by ADM, n =28) and Marlex mesh group (the ventral hernia repaired by Marlex mesh group, n = 10). The occurrence of ventral hernia was observed on postoperative 1 week. Six rats were respectively selected from the Marlex group and ADM group and tension resistance at 5weeks after surgery was observed. Results The occurrence of ventral hernia in ADM group and Marlex mesh group was significantly lower than that of ventral hernia group(0.04%, 0.0%, 100.0%, P ＜ 0. 01 ) in postoperative 1week, but there was no difference between ADM group and Marlex mesh group ( P ＞ 0.05 ). Marlex had a significantly higher breaking strength than ADM[(417 ±44)N vs( 111 ±27)N,P ＜0.01]. But 5 weeks after surgery, the tension resistance of ADM-fascial interface was higher than that of the Marlex-fascial [( 103 ± 27 ) N vs(71 ± 19 ) N,P ＜ 0. 05]. Conclusion As a material of repairing ventral hernia, ADM prepared by the complex maceration may be superior to Marlex mesh.     

糖尿病大鼠甲状腺组织降钙素免疫阳性滤泡旁细胞的变化

Objective To investigate the change of the parafollicular cells (C cell) in the thyroid of diabetes mellitus ( DM ) rats. Methods Fourteen male W rats were divided randomly into DM experiment group ( n = 8 )and normal (N) group ( n = 6). DM rats were sacrificed when 12 weeks after administration. Then the change of the number of calcitionin-positive C cells was investigated by immunohistochemistry method. Results Calcitionin-positive C cells in the thyroid of DM rats were round or ellips and were distributed separately or concentratively between the thyroid follicle and follicle epithelial cells. Under the high power lens, the C cell count of DM group was 37.400 ± 6. 533, which is more than the C cell count of N group ( 32.453 ± 4.5117 ). Under the lower power lens, The ratio of the positive reactive area and total area of the field of view in DM group was 0. 012 612 ±0. 0047 273, which was also higher than that of the N group(0.009 398 ±0.005 228). Both the distinctions had statistical significance.Conclusion The number of parafollicular cells increases in the thyroid of DM rats.     

糖尿病大鼠甲状腺组织降钙素免疫阳性滤泡旁细胞的变化

Objective To investigate the change of the parafollicular cells (C cell) in the thyroid of diabetes mellitus ( DM ) rats. Methods Fourteen male W rats were divided randomly into DM experiment group ( n = 8 )and normal (N) group ( n = 6). DM rats were sacrificed when 12 weeks after administration. Then the change of the number of calcitionin-positive C cells was investigated by immunohistochemistry method. Results Calcitionin-positive C cells in the thyroid of DM rats were round or ellips and were distributed separately or concentratively between the thyroid follicle and follicle epithelial cells. Under the high power lens, the C cell count of DM group was 37.400 ± 6. 533, which is more than the C cell count of N group ( 32.453 ± 4.5117 ). Under the lower power lens, The ratio of the positive reactive area and total area of the field of view in DM group was 0. 012 612 ±0. 0047 273, which was also higher than that of the N group(0.009 398 ±0.005 228). Both the distinctions had statistical significance.Conclusion The number of parafollicular cells increases in the thyroid of DM rats.     

BMSCs和软骨细胞共培养并与纤维蛋白黏胶复合后移植修复关节软骨缺损

Objective To study the repairing effects of cartilage of rabbit articular defects grafting on compound of BMSCs and articular cartilage cell and fibrin sealant. Methods The model of defect of rabbit articular cartilage were divided into 4 group, group A was grafting compound of BMSCs and articular cartilage cell and fibrin sealant; group B was grafting BMSCs and articular cartilage cell; group C was grafting fibrin sealant. the Repaired tissues were evaluated with macroscopic observation,histological scores and immunohistochemical staining at week 84and 8 after transplantation. Results Repaired cartilage group A was better than thos of group B 、C and group B 、C were better than group D. O'Driscoll mark was group A: (20.43 ± 1.81); group B: (16.29 ± 2.63); group C: (10.57 ± 3.60); group D: (5.77± 3.30), differences were statistically significant(P＜ 0.01). Conclusion compound of BMSCs and articular cartilage cell and fibrin sealant grafting can repairing articular cartilage defecting.     

Therapeutic effect of Turkish galls extract on iga nephropathy model rats北大核心CSCD

OBJECTIVE: To investigate the effect of Turkish galls extract (TGE) on the expression of IgA in serum, urine and renal tissue of IgA nephropathy (IgAN) model rats. METHODS: Fifty healthy male Sprague Dawley rats were randomly divided into normal control group, IgAN model group, and TGE 75,150 and 300 mg · kg-1 groups, 10 rats per group. The model of IgAN rats was established with bovine serum albumin (BSA) + lipopolysaccharide (LPS)+carbon tetrachlorid (CCI4)for 12 weeks. From the 13th week, TGE was ig administrated once a day for 4 weeks. At the end of the 12th and 16th weeks, 24 h urine protein was measured by BCA method. At the end of the 16th week, serum and urinary IgA levels were measured by enzyme linked immunosorbent assay(ELISA), serum creatinine(SCR) and blood urea nitrogen (BUN) were detected by an automatic biochemical analyzer, and the renal pathological changes were evaluated with an Oxford classification scoring system. The deposition of IgA immune complex in the kidney was observed by immunofluorescence assay. RESULTS: At the end of 12th week, 24 h urine protein increased in all IgAN groups (P<0.05), compared with normal control group. At the end of 16th week, 24 h urine protein, IgA content in serum and urine,SCr and BUN content in serum, score in Oxford classification of renal tissue and deposition of IgA immune complex in the kidney in IgAN model group were all higher than in normal control group (P<0.05). Compared with IgAN model group, 24 h urine protein, IgA content in serum and urine and SCr content in serum were decreased in all TGE groups (P<0.05), and BUN content in serum and deposition of IgA immune complex in the kidney decreased in TGE 150 and 300 mg · kg-1 groups (P<0.05). The score in Oxford classification of renal tissue was decreased in TGE 300 mg.kg-1 group only. CONCLUSION: TGE has curative effect on IgAN model rats by reducing serum and urinary IgA and decreasing IgA immune complex deposition.     

中西医结合治疗腰椎间盘突出症的临床研究

Objective To investigate the curative effect of western combined with traditional Chinese medicine in treatment of acute lumbar disc herniation.Methods 200 patients with lumbar disc herniation were randomly divided into western combined with traditional Chinese therapy group(group A) and western medicine group(group B) ,each group 100 cases.B group was conducted with the general anti - inflammatory, dehydration, nerve pain and nutritional therapy,and A group was given the traditional Chinese medicine treatment on the base of B group.After 3 weeks treatment, the curative effect, VAS score and hospital stay were observed.Results A total effective rate 90.0% in group A was higher than B group 73.0% ( x2 = 3.987 ,P < 0.05); The scores of VAS after treatment 4 weeks[(1.1 ±1.23、0.9 ±0.6)/point]were lower than before treatment[(8.6 ± 1.43,8.8 ± 1.382)/point]in two groups(t =2.301,2.335,all P<0.05) ;The scores of VAS after treatment 3d and 1 week in group A[(5.5 ±0.92) point,(2.7±1.14) point] were lower than group B[(8.2 ±0.59) point,(5.3 ±0.91) point] (t =2.405,2.435,all P<0.05);The hospital days(20.1 ±3.5)d in group A was significantly lower than group B(27.6 ±4.3)d( t = 2.314,P <0.05).Conclusion Western combined with traditional Chinese medicine had better clinical efficacy in teatrent of patients with acute lumbar disc hemiation.     

实验性变态反应性脑脊髓炎大鼠L-选择素的表达及意义

Objective To investigate the effect of L-selectin in experimental allergic encephalomyelitis (EAE) of Wistar rats.Methods The rats were randomly divided into four groups;the normal group,the CFA group, the LMS group and the model group;The animal models were established in rats by immunization with myelin basic protein of spinal cord of guinea pig and complete Freund's adjuvant(CFA).The symptom of EAE was observed; pathological feature and myelin of brain and spinal were detected with HE stain and Loyez's stain respectively.The number of positive vessels of L-selectin expression was detected by immunohistochemistry.Results 100% experimental Wistar rats treated with MBP and levamisole developed EAE,but none of the other groups.The number of positive vessels of L-selectin expression was (31.86 ± 1.39) in model group, obviously higher than that of in the normal group (1.38 ±0.18) ,the CFA group( 1.50 ±0.27) and the LMS group(7.25 ±0.59) (all P <0.05) ;The inflammation cells were found around vessels and demyelination were seen in white matter of brain and spinal cords.Conclusion The expression of L-selectin should play an important role in EAE.     

噻托溴铵治疗稳定期慢性阻塞性肺疾病的疗效观察

Objective To explore the clinical effect of inhaling tiotropium in treatment of patients with stable chronic obstructive pulmonary disease.Methods The clinical data of 240 patients with stable chronic obstructive pulmonary disease were retrospectively analyzed,and they were divided into two groups,the group treated with tiotropium and the group treated with ipratropium bromide(n = 120 in each group).The lung function was detected 2 weeks before and after the treatment, and the clinical symptoms were observed.Results FVC, FEV1, FEV1/FVC, and FEV1/predictive value of the tiotropium group were(3.64 ±0.58) L, (2.53 ±0.25) L, (62.40 ±9.42)% ,(58.24 ± 4.83)% respectively,much higher than(3.14 ±0.36)L,( 1.91 ±0.23)L,(56.50 ±7.36)%,(50.41 ±5.54)% of the ipratropium bromide group(t =2.037,1.875,2.616,2.945,all P<0.05) ,and the clinical symptoms were better changed in tiotropium group than in ipratropium bromide group (H = 5.990, P < 0.05 ).Conclusion Tiotropium could effectively improve lung function and clinical symptoms in patients with stable COPD.     

危重症患者血清降钙素原测定对细菌感染的诊断价值

Objective To evaluate the clinical value of serum procalcitonin (PCT) in the diagnosis of bacte-rial infections in the critically ill patients. Methods A loud d 100 cases of critical patients were divided into bacteri-al infection group(56 cases) and non-bacterial infection group(44 cases). Serum PCT was measured by immunolu-minometric assay. Results The concentration of PCT in bacterial infection group(21.54 ±5.72) μg/L, was signifi-cantly higher than non-bacterial infection group (11. 95±4. 58)μg/L (t =2.291,P<0.05);The APACHE Ⅲ score of bacterial infection group(62. 44 ± 19. 55) cent was significantly higher than non-bacterial infection group(44. 56 ± 25. 88) cent(t = 2. 195 ,P < 0. 05). The concentration of PCT of 1.0μg/L and 2. 0 p.g/L compared to the former sensitivity (96. 5) % higher than the latter (55.2) % (X2 = 3. 94, P < 0. 05), the former specificity (41.7) % lower than the latter (95.8)% (X2 = 4. 02 ,P < 0. 05);1.5μg/L as a positive standard Youden index and the Agreement (83.0 % ,0. 65), were significantly higher than 1 μg/L and 2 μg/L(71.7%, 0. 38 ;73.6% ,0. 51) (X2 = 3.84, X2=3. 90,X2 = 3.992 = 3.91 ,P < 0. 05);The concentration of PCT in death group (38. 9 ±12. 6)μg/L was signifi-canfly higher than the survival group(11.8± 8. 3) μg/L(t =2. 398 ,P <0. 05). Conclusion Serum PCT has clinical values in the diagnosis and therapy of bacterial infections in the critical patients.