共查询到17条相似文献,搜索用时 140 毫秒
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目的:考察石见穿不同提取部位的体外抗肿瘤活性,从而确定其体外抗肿瘤作用的活性部位。方法:使用95%工业酒精对石见穿饮片进行提取,然后分别用石油醚、乙酸乙酯、氯仿和正丁醇依次对乙醇提取部位进行分段萃取,采用MTT法(四甲基偶氮唑蓝比色试验)测定各个提取部位对体外培养的5株人肿瘤细胞的增殖抑制作用。结果:在体外,氯仿部位和乙酸乙酯部位对于5株人肿瘤细胞株具有较强的增殖抑制作用。结论:氯仿部位、乙酸乙酯部位是石见穿发挥体外抗肿瘤作用的主要活性部位。 相似文献
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中药石见穿提取物熊果酸体外抑制肿瘤细胞增殖的实验研究 总被引:1,自引:0,他引:1
目的:考察从石见穿醇提物氯仿部位提取分离得到的熊果酸对5株人肿瘤细胞株的体外增殖抑制作用,为开发新的抗癌药物提供理论依据。方法:使用95%工业酒精对石见穿饮片进行提取,然后分别用石油醚、乙酸乙酯、氯仿和正丁醇依次对乙醇提取部位进行分段萃取,采用系统溶剂法、梯度洗脱、柱色谱分离、重结晶等方法,从石见穿醇提物氯仿部位中分离得到熊果酸。采用MTT法测定熊果酸对体外培养的5株人肿瘤细胞株的增殖抑制作用。结果:在体外,熊果酸对于5株细胞株具有较强的增殖抑制作用。结论:熊果酸是石见穿醇提物、氯仿部位发挥体外抗肿瘤作用的主要有效成分之一。 相似文献
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Flt3配体(FL)是一种能够刺激早期造血的细胞因子。FL可促进体内树突状细胞、自然杀伤细胞、细胞毒T淋巴细胞的增殖、分化和成熟,从而具有重要的抗肿瘤免疫治疗作用。对乳腺癌、纤维母细胞肉瘤、肝癌、淋巴瘤等肿瘤的体内、外实验亦证实FL对肿瘤生长的抑制作用。本文就FL的主要生物学特性、抗肿瘤作用机制及其在体应用研究近况作一综述。 相似文献
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锂盐原来作为治疗狂郁症及白细胞减少症的药物被临床应用。1991年koryora发现Licl可以缓解和减轻化疗所造成的粒—巨细胞生成的抑制。由此,关于Licl在肿瘤治疗中应用的研究逐渐深入,但多限于体外研究,体内抗肿瘤作用的报道很少。本文报道了不同剂量的Licl体内抗肿瘤增长的作用。 相似文献
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丹参是活血化瘀类中药的代表药物,近年来随着对丹参研究的不断深入发现其具有抗肿瘤转移作用。目前的研究提示丹参主要通过提高免疫功能、诱导分化和影响细胞黏附分子的表达而抑制肿瘤的转移。随着丹参抗肿瘤机制的阐明,丹参有望成为防治肿瘤转移的有效药物。 相似文献
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目的 探讨丹参川芎制剂对甲状腺癌术后低钙血症和甲状旁腺功能减退的疗效。
方法 选择2016年1月至2017年12月江苏省肿瘤医院头颈外科收治的30例甲状腺癌术后并发低钙血症和甲状旁腺功能减退患者。遵患者意愿,接受丹参川芎注射液治疗为观察组,共15例,在传统葡萄糖酸钙注射液和骨化三醇治疗基础上加用丹参川芎注射液;对照组予传统葡萄糖酸钙注射液和骨化三醇治疗,共15例。分析丹参川芎制剂对术后甲状旁腺功能减退的治疗效果。
结果 观察组和对照组术后第1天血钙水平相比,差异无统计学意义(P>005);观察组术后第3、7、30天血钙水平高于对照组,差异有统计学意义(P<005)。观察组和对照组术后第1、3天血甲状旁腺激素(PTH)水平相比,差异无统计学意义(P>005);观察组术后第7、30天血钙水平高于对照组,差异有统计学意义(P<005)。
结论 丹参川芎制剂对术后甲状旁腺功能减退及低钙血症治疗效果明显,且不良反应低,值得临床应用。 相似文献
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目的:研究红景天、丹参及荷花粉对光气损伤小鼠的治疗作用。方法:50只小鼠随机分为红景天、丹参、荷花粉治疗组,阳性对照组和阴性对照组,共5组。阴性对照组以空气为对照,其余各组小鼠给予11.9 mg/L的光气1 min,于染毒后20min,各治疗组小鼠分别以0.01ml/g的红景天、丹参和荷花粉灌胃,阳性对照组给予等体积生理盐水,4 h后解剖小鼠取样,测定支气管肺泡灌洗液中蛋白含量、肺脏MDA及GSH含量及进行血细胞测定。结果:红景天治疗组小鼠支气管肺泡灌洗液中蛋白含量显著低于阳性对照组(P〈0.05);红景天和丹参治疗组小鼠全血血小板较阳性对照组显著升高(P〈0.05);各治疗组小鼠肺脏MDA含量均较阳性对照组降低,其中红景天组最为显著(P〈0.05);各治疗组小鼠肺脏GSH含量均较阳性对照组升高,其中红景天组最为显著(P〈0.05)。结论:红景天、丹参和荷花粉均对光气导致的肺损伤具有一定的治疗作用,红景天的治疗作用优于丹参和荷花粉。 相似文献
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Thelen P Scharf JG Burfeind P Hemmerlein B Wuttke W Spengler B Christoffel V Ringert RH Seidlová-Wuttke D 《Carcinogenesis》2005,26(8):1360-1367
Isoflavones have been shown to exert antiproliferative effects on cancer cells by steroid receptor signaling. In this study, we demonstrate the potential of plant constituents extracted from Belamcanda chinensis as anticancer drugs, which regulate the aberrant expression of genes relevant in proliferation, invasion, immortalization and apoptosis. LNCaP cells were treated with B.chinensis extract, tectorigenin or other isoflavones and mRNA expression was quantified by using real time RT-PCR. In addition, ELISA, TRAP assays and western blots were used to measure protein expression or activity. Male nude mice (n=18) were injected subcutaneously with LNCaP cells and were fed with extracts from B.chinensis, and tumor development was monitored versus a control animal group (n=18). Tectorigenin and several other phytochemicals downregulated PDEF, PSA and IGF-1 receptor mRNA expression in vitro. Furthermore, PSA secretion and IGF-1 receptor protein expression were diminished, and hTERT mRNA expression and telomerase activity decreased after tectorigenin treatments. However, TIMP-3 mRNA was upregulated on tectorigenin treatment. Growth of subcutaneous tumors in nude mice was delayed and diminished in animals fed with extracts from B.chinensis. The downregulation of PDEF, PSA, hTERT and IGF-1 receptor gene expression by tectorigenin demonstrates the antiproliferative potential of these agents. The upregulation of TIMP-3 gene expression indicates a pro-apoptotic function of the drug and a reduction of the invasiveness of tumors. The animal experiments demonstrate that B.chinensis markedly inhibited the development of tumors in vivo. Thus, these compounds may be useful for the prevention or treatment of human prostate cancer. 相似文献
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《Asian Pacific journal of cancer prevention》2015,16(4):1501-1506
Gemcitabine is an anti-cancer drug with clinically uses in the treatment of various neoplasms, includingbreast, ovarian, non-small cell lung, pancreaticand cervical cancers, T-cell malignancies, germ cell tumours, andhepatocellular carcinomas. However, it has also been reported to have many adverse effects. Naturally occurringanti-mutagenic effects, especially those of plant origin, have recently become a subject of intensive research.The present study was therefore designed to investigate the anti-mutagenic effects of Salvia merjamie (Family:Lamiaceae) plant extracts against the mutagenic effects of gemcitabine. The anti-mutagenic properties of Salviamerjamie were tested in Inbred SWR/J male and female mice bone marrow cells. The mice were treated in fourgroups; a control group treated with 30 mg/kg body weight gemcitabine and three treatment groups, each with 30mg/kg body weight gemcitabine together with, respectively, 50, 100 and 150 mg/kg body weight Salvia merjamieextract. Chromosomal aberration and mitotic index assays were performed with the results demonstrating thatSalvia merjamie extract protects bone marrow cells in mice against gemcitabine induced mutagenicity. Thisinformation can be used for the development of a potential therapeutic anti-mutagenic agents. 相似文献
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目的 探讨白头翁醇提物对体外血管生成的抑制作用及其可能机制。方法 采用MTT法观察白头翁醇提物对人脐静脉血管内皮细胞(HUVEC)和人肠癌LoVo细胞增殖的影响;通过Transwell小室趋化实验、体外小管形成实验观察白头翁醇提物对HUVEC迁移、形成血管能力的影响;采用流式细胞仪检测白头翁醇提物对HUVEC的凋亡率及细胞周期的影响。结果 2~8μg/ml的白头翁醇提物作用48h对HUVEC的增殖抑制率为21.6%~72.0%,对LoVo细胞的增殖抑制率为13.2%~22.9%;体外小管形成实验发现,2~8μg/ml白头翁醇提物作用24h,HUVEC小管形成数目减少,且管腔不完整,与对照组比较差异有统计学意义(P<0001)。经2~8μg/ml白头翁醇提物处理12h,HUVEC迁移数明显少于对照组(P<0001)。2、4、8μg/ml白头翁醇提物作用于HUVEC细胞48h后,其凋亡率分别为952%、1864%和2007%,对照组为6.25%。8μg/ml白头翁醇提物作用于HUVEC细胞24h后,细胞周期停滞在G2/M期。结论 白头翁醇提物在体外能有效抑制血管生成,其机制可能与抑制HUVEC增殖、迁移和小管形成,诱导HUVEC凋亡,抑制HUVEC有丝分裂有关。 相似文献