过敏原特异性IgE在诊治湿疹患儿中的应用价值

目的探讨过敏原特异性Ig E在诊治湿疹患儿中的应用价值。方法选取我院2017年5月～2018年5月湿疹患儿211例,入院后第2天晨起时抽取空腹静脉血4ml,以免疫印迹法检测食入性及吸入性过敏原特异性IgE。统计分析本组过敏原特异性IgE检测情况,并比较分析不同年龄湿疹患儿过敏原特异性IgE检测情况。结果本组211例湿疹患儿中最常见吸入性过敏原为户尘螨(31.75%)、屋尘(22.27%)等;最常见食入性过敏原为牛奶(36.49%)、鸡蛋白(20.38%)、牛肉(19.91%)等。1岁过敏患儿过敏原特异性IgE阳性率由高至低分别为牛奶、鸡蛋白、牛肉;(1～3)岁过敏患儿过敏原特异性IgE阳性率由高至低分别为牛奶、尘螨、鸡蛋白、狗毛皮屑; 3岁过敏患儿过敏原特异性IgE阳性率由高至低分别为尘螨、屋尘、牛奶、牛肉及狗毛皮屑,且不同年龄过敏患儿尘螨、屋尘、鸡蛋白、牛奶阳性率间存在显著差异(P 0.05)。结论过敏患儿过敏原特异性IgE阳性表达率较高,其在疾病发生及进展中具有重要作用,可通过测定过敏原特异性IgE含量对疾病予以诊断,据此及早制定、调整治疗方案,利于改善疾病疗效及预后。     

儿童湿疹872例血清过敏原特异性IgE检测结果分析

目的:检测湿疹患儿常见的过敏原,寻找儿童湿疹可能的过敏促发因素,为其治疗及预防提供依据。方法:采用Mediwiss公司AllergyScreen Analytic GmbH过敏原检测系统,对872例湿疹患儿进行血清特异性IgE(specific IgE,sIgE)和总IgE检测,并在不同年龄组间进行比较。结果:872例湿疹患儿slgE阳性690例(79.13%),总IgE阳性709例(81.30%)。其中吸入性过敏原最常见的依次为户尘螨(34.60%)、点青霉(22.71%)、屋尘(16.28%)和狗毛皮屑(10.89%);食物性过敏原中,最常见的依次为牛奶(36.12%)、羊肉(22.94%)、鸡蛋白(20.07%)和牛肉(13.88%)。比较1岁组,1~3岁组和3岁组,户尘螨、屋尘、狗毛皮屑、点青霉、鸡蛋白、牛奶、羊肉和蟹的sIgE阳性率的差异有统计学意义(P0.05)。结论:户尘螨、点青霉和屋尘是湿疹患儿最主要的吸入性过敏原,牛奶、羊肉、鸡蛋白是湿疹患儿最常见的食物性过敏原。随年龄增大,吸入性过敏原逐步取代食物成为主要过敏原。血清sIgE检测为湿疹患儿提示了相关过敏原,可以针对性地回避相关过敏原。     

湿疹及皮炎

20061975149例湿疹皮炎患者血清特异性IgE的检测分析/水润英(上海华东医院皮肤科),吴跃申,徐琴言…∥中国中西医结合皮肤性病学杂志.-2006,5(1).-41~42采用IVT过敏原体外检测试剂盒,对149例湿疹皮炎患者(季节性皮炎、过敏性皮炎)血清特异性IgE测定,结果:IVT704(吸入组)阳性85例,占57·05%;IVT702(食物组)44例阳性,占29·53%,两组阳性率经统计学处理,P<0·05。IVT702(食物组)与IVT704(吸入组)均阳性的有24例,占总数的16·11%;有明确IVT702(食物组)或IVT704(吸入组)过敏原阳性的患者105例,占总数的70·47%。提示吸入性过敏原在湿疹…     

149例湿疹皮炎患者血清特异性IgE的检测分析

我们从2005年1月起采用IVT过敏原体外检测试剂盒,对上海地区149例湿疹皮炎患者(季节性皮炎、过敏性皮炎)血清特异性IgE测定,现将结果报告如下.     

反复发作性过敏性紫癜患儿血清过敏原特异性IgE检测

目的进一步了解血清过敏原特异性IgE在儿童反复发作性过敏性紫癜中的作用,探讨各种变应原与疾病发病的关系。方法采用酶联免疫吸附试验检测130例反复发作性过敏性紫癜患儿的血清过敏原特异性IgE。结果130例患儿中血清总IgE水平>50IU/ml者103例,阳性率80.47%。其中73例(75.3%)对两种以上过敏原呈阳性反应,30例(24.7%)对一种过敏原呈阳性反应。常见的吸入性过敏原为霉菌(29/130)、蒿类花粉(16/130)和尘螨(15/130)。食物组最常见的为牛奶(35/130)、蛋白类(26/130)、鱼类(15/130)和虾蟹(12/130)。结论反复发作性过敏性紫癜的发生与吸入性和食入性过敏原都有一定的相关性,霉菌和蛋白类食物是诱导反复发作性过敏性紫癜的主要致病原。特异性IgE检测可帮助筛选过敏原,对疾病的预防具有积极意义。     

昆明地区2110例过敏性皮肤病患者特异性过敏原IgE检测结果分析

目的 分析昆明地区2 110例过敏性皮肤病患者过敏原s IgE分布特点,为临床提供流行病学数据。方法 回顾性分析昆明医科大学第一附属医院皮肤科2020年7月至2021年6月临床2 110例过敏性皮肤病患者的影响因素及其过敏原sIgE检测结果。结果 在2 110例过敏性皮肤病患者中,荨麻疹占53.5%,皮炎类疾病（特应性皮炎、接触性皮炎和其他皮炎）占26.9%,湿疹类疾病（手部湿疹、泛发性湿疹等）占19.6%,男女比例为1∶1.96,以青年段和中年段为主,夏季是高发季节,占比为31.33%。荨麻疹患者发病高峰为7月和8月,皮炎类疾病为3月和8月,湿疹类疾病为3月和10月。sIgE检测结果显示：过敏性皮肤病阳性率达32.65%,其中荨麻疹阳性率35.82%、皮炎类疾病阳性率29.05%,湿疹类疾病阳性率28.99%。过敏性皮肤病患者中动物皮毛组的阳性率最高（24.60%）,其中荨麻疹比例最高（P <0.05）。不同年龄段对不同过敏原的阳性率比较显示,儿童阳性率均最高,与青年组及老年组比较差异有统计学意义（P <0.01）。在不同季节里,食入性坚果类组和室内常见吸入性过敏原组在夏...     

257例儿童荨麻疹血清特异性过敏原IgE检测结果分析

目的:探寻荨麻疹患儿常见的过敏原种类,为其治疗及预防提供依据.方法:采用Mediwiss 公司AllergyScreenAnalytic GmbH 过敏原检测系统,对257 例荨麻疹患儿进行血清特异性IgE(specific IgE,sIgE)和总IgE 检测,并在不同年龄组间进行比较.结果:257例荨麻疹患儿sIgE阳性142例(55.27%),总IgE阳性率69.65%.其中吸入性过敏原最常见的依次为户尘螨18.68%、霉菌组合17.51%、猫毛皮屑4.67%、屋尘4.28%和狗毛皮屑3.89%;食物性过敏原中,最常见的依次为牛奶28.79%、羊肉16.34%、牛肉7.00%、鸡蛋白5.45%和虾3.11%.比较＜3 岁组、3～6 岁组和＞6 岁组,户尘螨、猫毛皮屑、牛奶和羊肉sIgE阳性率的差异有统计学意义(P ＜ 0.05).结论:尘螨和霉菌是荨麻疹患儿最主要的吸入性过敏原,牛奶、牛羊肉、鸡蛋白是荨麻疹患儿最常见的食物性过敏原.血清sIgE检测为荨麻疹患儿提示了相关过敏原,可以针对性地回避相关过敏原.     

湿疹皮炎患者过敏原特异性IgE检测阳性结果的多中心研究

目的 探讨湿疹皮炎患者血清过敏原检测常见的阳性过敏原及其临床意义。 方法 回顾性分析中国10家医院皮肤科就诊并进行了血清变应原特异性IgE检测且至少一项阳性的湿疹皮炎患者。 结果 458例患者纳入研究,其中男209例（45.63%）,女249例（54.37%）,年龄（28.13 ± 23.11）岁。前3位常见阳性过敏原为蟑螂（180/458,占39.30%）、尘螨组合（152/458,占33.19%）、花生（116/458,占25.33%）。89.66%（26/29）特应性皮炎患者及86.84%（33/38）面部湿疹患者存在多种过敏原。皮损相对泛发者过敏率（88/110,占80%）较皮损相对局限者（245/348,占70.40%）高,差异有统计学意义（χ2 = 3.880,P = 0.049）。吸入变应原阳性率在11 ~ 20岁年龄组最高（39/43,占90.70%）;牛奶（26/98,占26.53%）、牛肉（19/98,占19.39%）,特异性IgE 阳性多见于3岁以下婴幼儿。北方与南方常见变应原不同,艾蒿、蟑螂、牛奶、黄豆、牛肉、淡水鱼特异性IgE阳性率（10.35%、42.42%、14.39%、13.89%、11.36%、13.38%）均分别高于南方（1.61%、19.35%、3.23%、3.23%、1.61%、3.23%）,差异有统计学意义（?字2 = 4.917、11.959、5.958、5.594、5.641、5.235,均P < 0.05）。 结论 蟑螂是湿疹皮炎患者致敏率较高的变应原,临床意义值得研究。面部湿疹及特应性皮炎更容易多发过敏。年龄是影响变应原致敏的较重要因素。不同地域常见变应原不同。     

过敏性紫癜患者血清过敏原特异性IgE检测

我们采用酶联免疫吸附试验检测100例过敏性紫癜患者的血清过敏原特异性IgE,以进一步了解血清过敏原特异性IgE在过敏性紫癜中的作用,探讨各种变应原与疾病发生的关系。100例患者中血清总IgE水平>50IU/mL者64例,阳性率64%。其中对两种以上过敏原呈阳性反应者19例(29.7%),对一种过敏原呈阳性反应者45例(70.3%)。吸入组中特异性IgE阳性最常见的致敏原为霉菌(21/100),其次为蒿类花粉(6/100)和尘螨(7/100)。食物组最常见的为牛奶(16/100),其次为蛋白类(8/100)、鱼类(7/100)和虾蟹(6/100)。过敏性紫癜的发生与吸入性和食物性过敏原都有一定的相关性,霉菌和蛋白类食物是诱导过敏性紫癜复发的主要变应原。特异性IgE检测可帮助筛查过敏原,对疾病的预防具有积极意义。     

155例眼睑皮炎患者斑贴试验结果分析

目的：明确眼睑皮炎的常见变应原。方法：回顾分析我院皮肤科2018年155例眼睑皮炎患者斑贴试验资料。结果：155例眼睑皮炎患者中85例斑贴试验阳性, 总阳性率为54.8%。其中对1项过敏原反应阳性者27例（31.8%）,2项过敏原阳性者19例（22.4%）,3项过敏原阳性者14例（16.5%）,4项及以上过敏原阳性者25例（29.4%）。阳性率较高的前3位过敏原依次是：硫酸镍（24例,9.4%）,甲基异噻唑啉酮（24例,9.4%）,纺织染料混合物（14例,5.5%）。＜20岁,20～39,40～59,≥60岁患者斑贴试验阳性率分别为37.5%,54.2%,65.0%,18.2%,不同年龄组阳性率结果具有统计学意义(χ2=8.483,P＜0.05）。结论：甲基异噻唑啉酮、硫酸镍、纺织染料混合物是眼睑皮炎患者最常见的变应原,变应原阳性率的高低与患者的年龄有一定关联。     

Relationship between skin bacterial colonization and the occurrence of allergen-specific and non-allergen-specific antibodies in sera of children with atopic eczema/dermatitis syndrome

In this study we investigated skin bacterial colonization, allergen-specific IgE and antiphospholipid/antinuclear antibodies in 72 children with atopic eczema/dermatitis syndrome (age 2-17 years). Bacteria were found on the skin in 41 cases and serological allergen-specific IgE positivity in 37. The different forms of antibodies appeared in the ratio 21/72 (33 antibodies in 21 children). The occurrence of antiphospholipid antibodies was significantly higher in the patients than in the controls. There were significantly more allergens in the group with bacterial colonization than in the group without colonization. The SCORAD index showed a significant positive association with the skin colonization. We conclude that there are significant relationships between the occurrence of Staphylococcus aureus colonization and the levels of inhalant allergen-specific IgE in children with atopic eczema/dermatitis syndrome, and between the occurrence of antiphospholipid IgM positivity and atopic eczema/dermatitis syndrome.     

Correlation of nipple eczema in pregnancy with atopic dermatitis in Northern India: a study of 100 cases

BackgroundNipple eczema is a less common presentation of atopic dermatitis. No studies in the literature have correlated nipple eczema in pregnancy as a manifestation of atopic dermatitis.ObjectiveTo evaluate whether nipple eczema presenting in pregnancy is a manifestation of atopic dermatitis.MethodsThis was a prospective observational study including 100 women who presented with nipple eczema for the first time during pregnancy. The exclusion criteria were any patient with previous history of nipple eczema, those already on oral or topical treatment for atopic dermatitis or nipple eczema, and other disorders mimicking eczema. Patients were divided into two groups ‒ nipple eczema with atopic dermatitis and without atopic dermatitis. Demographic data, clinical features, total leukocyte count, differential leukocyte count, absolute eosinophil counts, and serum IgE levels were compared between the two groups to detect association between nipple eczema in pregnancy and atopic dermatitis.ResultsOut of 100 patients, 39 were diagnosed with atopic dermatitis, whereas 61 were ruled out to have any features suggestive of atopic dermatitis. There were no statistically significant differences in mean age, mean duration of symptoms, and serum IgE levels. In patients with atopic dermatitis, bilateral symptoms were noted more commonly than in patients without the disease, but this was statistically insignificant.Study limitationsLack of long term follow-up and no large studies in literature to compare results.ConclusionNipple eczema in pregnancy follows a similar pattern as in other age groups. The clinical profile of patients is similar in cases with and without atopic dermatitis.     

新疆地区皮炎及湿疹患者斑贴试验结果分析

目的：确定新疆地区皮炎及湿疹患者的常见接触性变应原.方法：采用斑贴试验试剂盒对202例患者进行了斑贴试验.结果：202例患者中有122例至少对一种物质过敏,总阳性率为60.40%,阳性率较高的几种变应原依次为硫酸镍39例（19.31%）,白降汞31例（15.35%）和橡胶24例（11.88%）.结论：新疆地区皮炎及湿疹主要变应原为3种化学物质.     

T-cell subset assay. A useful differentiating marker of atopic and seborrheic eczema in infancy?

To confirm T-cell changes previously reported in atopic eczema, to compare T-cell subset values in atopic and seborrheic infants, and to determine whether T-cell subset assay would be a useful differentiating marker between atopic and seborrheic infants, three age- and sex-matched groups of normal, seborrheic, and atopic infants were recruited. T-cell subset values, total serum IgE, and serum IgE to specific allergens (Dermatophagoides pteronyssimus, cat epithelium, dog dander, rye grass, egg white, and milk) were measured. The results showed that total and allergen-specific IgE levels were higher in atopic disease in infancy, but no statistical differences in T-cell subset values between seborrheic and atopic infants were found. T-cell subset values are therefore not a reliable differentiating marker between atopic and seborrheic eczema in infancy.     

Correlation among skin prick test, total and specific IgE UniCAP tests in atopic patients from Zagreb, Croatia

The correlation of pollen allergens, Dermatophagoides pteronyssinus and animal dender was assessed during a two-year period. Results of skin prick test, total and specific IgE UniCAP tests were compared in atopic patients (AP) with the following diagnoses: atopic dermatitis, allergic rhinitis, allergic conjunctivitis, allergic bronchitis or asthma, allergic urticaria and angioedema. The study included total and specific IgE (in vitro) tests to allergen mixtures (grass, tree, weed) or to single allergens of Dermatophagoides pteronyssinus (Der p), cat and dog fur, feather, etc. Comparison of skin prick test with total and specific IgE UniCAP immunoassay was done in 173 patients, i.e. 107 female and 66 male atopic patients aged 9-76 years. Allergies were most commonly recorded in the 25-35 age group. Total IgE ranged from 8.63 kU/l to >4000 kU/l, with specific IgE ranging from class 1 to class 5. Skin prick test showed high correlation with specific IgE for grass and weed pollen in patients with repiratory allergy (50.28%). Good correlation among all three tests was quite frequently observed. The results suggest that the study should be continued using these three tests in further cases of atopic dermatitis.     

不同部位的慢性湿疹与斑贴试验相关性研究

皮炎湿疹与多种变应原之间存在联系,并且发现不同部位的皮炎湿疹的常见变应原各不相同。引起面部湿疹和皮炎的致敏原主要为硫酸镍、重铬酸钾、对苯类、芳香混合物,手部主要为硫柳汞、芳香混合物、重铬酸钾、对苯二胺,脐周主要为硫酸镍。斑贴试验有助于明确慢性湿疹和皮炎患者的接触性致敏原,并发现不同部位皮炎和湿疹常见致敏原的不同。提示临床上应根据不同部位的皮炎湿疹采取不同措施。     

816例皮肤点刺试验过敏原结果分析

目的 探讨特应性皮炎、慢性荨麻疹、慢性湿疹常见过敏原的可能致病情况.方法 用标准变应原液在前臂屈侧皮肤进行皮肤点刺试验,分别用0.1%组胺和0.9%氯化钠液作阳性和阴性对照,统计分析采用x2检验.结果 尘螨(屋尘螨+粉尘螨)的阳性率最高,达50.0%～78.6%,其次为动物毛,达7.1%～21.4%,霉菌的阳性率也较高,达7.1%～14.3%,并且这3种变应原在3种疾病间的阳性率差异均有统计学意义(P＜0.01),以特异性皮炎的阳性率最高.食物组过敏原中虾的阳性率(6.6%～14.3%)最高,但在3种疾病间的阳性率差异无统计学意义(P＞0.05);金枪鱼在慢性荨麻疹中的阳性率(9.5%)较高,鸡蛋、牛奶、芹菜在特异性皮炎中的阳性率(7.1%～14.3%)较高,这4种变应原在3种疾病间的阳性率差异均有统计学意义(P＜0.05).结论 吸入组过敏原是慢性过敏性皮肤病的常见过敏原,尘螨为首要过敏原,以特异性皮炎的阳性率为最高.     

过敏性皮肤病患者斑贴试验结果分析及临床意义

目的研究过敏性皮肤病患者斑贴试验结果及临床意义.方法对254例湿疹、56例慢性荨麻疹、28例特应性皮炎患者进行斑贴试验检测.结果三组患者中阳性率较高的致敏原有四种物质相同,分别为硫酸镍、重铬酸甲、卡巴混合物、芳香混合物;特应性皮炎组阳性率(64.29%)明显低于湿疹组(79.92%),慢性荨麻疹(76.79%)与湿疹二组阳性率之间差异无显著性.结论过敏性皮肤病患者阳性率较高的致敏原具有相同性;硫酸镍、重铬酸甲、卡巴混合物、芳香混合物具有较高的致敏性;斑贴试验主要用于检测外源性变应原,特应性皮炎的病因可能以内源性为主;接触性过敏原可以引起接触性荨麻疹;斑贴试验阳性致敏原常是引起面部湿疹、手湿疹的致病因素.     

Sensitizing agents found in children and adolescents with recalcitrant atopic dermatitis: a cross-sectional study with a pediatric battery

BackgroundAtopic dermatitis is the most common inflammatory skin disease in childhood and has an important impact on quality of life, especially severe cases or those that are recalcitrant to treatments. Sensitization to allergens with the potential for allergic contact dermatitis is a factor associated with cases of recalcitrant atopic dermatitis. Understanding the relationship between atopic dermatitis, allergens, and allergic contact dermatitis is essential. In Brazil, there are no studies on sensitization to allergens found in patch tests with pediatric batteries in patients with atopic dermatitis.ObjectivesTo verify the main sensitizing agents, the prevalence of allergic contact dermatitis and the epidemiological and clinical profile of children and adolescents with atopic dermatitis.MethodsCross-sectional, prospective study in patients between 4 and 18 years of age, with recalcitrant atopic dermatitis, treated at the Sanitary Dermatology Outpatient Clinic (RS). All patients underwent patch tests with a battery of pediatric allergens.ResultsThe prevalence of sensitization and allergic contact dermatitis in the evaluated patients was 37.07% (20/54) and 27.7% (15/54), respectively. The most frequent allergens were: nickel sulfate (16.7%), disperse blue (5.6%), and fragrance mix I (5.6%). Nickel was associated with the female sex (p = 0.019).Study limitationsSample size and selection, absence of a control group.ConclusionsA proportion of patients with recalcitrant atopic dermatitis may be sensitized to different allergens and may even have developed allergic contact dermatitis. Recognizing this context is important in the prevention strategy and management of the disease.