腮腺肿瘤患者唾液和血液中CEA、CA125含量的检测及其在肿瘤组织中的表达

目的：检测腮腺肿瘤患者唾液和血液中CA125、CEA的含量及其在腮腺肿瘤组织中的表达。方法选择腮腺良性肿瘤患者83例,腮腺恶性肿瘤患者18例,对照组为30名健康志愿者。采用化学发光法( CLIA)检测唾液及血液样本中CEA和CA125含量。采用免疫组化法检测腮腺肿瘤组织中CEA和CA125的表达。结果腮腺肿瘤患者混合唾液中CEA和CA125水平明显高于对照组。腮腺肿瘤患者与对照组血液中CEA和CA125水平无显著差异。良、恶性肿瘤患者唾液中CEA和CA125含量无显著差异。 CEA及CA125的分布特点为,混合唾液中含量最高,血液中含量最低。相关性分析显示,混合唾液中CEA与CA125呈现明显正相关性( r=0.652-0.913)。在大部分腮腺肿瘤组织中,CEA和CA125均为阳性表达,而且部分病例的肿瘤组织周围正常腺体也可见CEA及CA125的阳性表达。结论唾液中CEA和CA125水平的变化可以反映腮腺肿瘤的存在,但无法区分腮腺肿瘤的良恶性。血液中CEA和CA125不能反映腮腺肿瘤的存在。腮腺肿瘤患者的混合唾液样本中CEA与CA125呈现同步变化的趋势。唾液中CEA与CA125可能主要来源于涎腺腺体及肿瘤组织,而不是来自血液。     

糖尿病患者胰岛素治疗前后唾液葡萄糖含量分析

目的:研究糖尿病患者胰岛素治疗前后唾液中葡萄糖含量的改变,并观察唾液糖与血糖的关系。方法:实验组为40例糖尿病患者。收集糖尿病患者胰岛素治疗前后清晨非刺激状态下自然分泌的全唾液,进行葡萄糖含量分析,同时行空腹血糖分析。结果:治疗前患者的唾液葡萄糖含量为(2.081±0.287)mmol/L,高于治疗后的(1.571±0.193)mmol/L,二者有显著性差异(P<0.01);治疗前患者的唾液葡萄糖含量与血糖浓度有显著直线相关性(P<0.05);治疗后患者的唾液葡萄糖含量与血糖浓度无显著直线相关性(P>0.05)。结论:糖尿病患者唾液中的葡萄糖含量明显增加,说明唾液腺分泌功能发生改变;良好的胰岛素治疗不仅可以达到良好的血糖控制,还可以控制减少唾液葡萄糖的分泌量。     

代谢综合征患者与健康者唾液中肿瘤坏死因子α、白细胞介素1β水平比较

目的 比较代谢综合征(metabolic syndrome,MS)患者和非MS的健康对照者唾液中肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-1β水平,了解牙周炎与MS患者体内低度炎症的关系.方法 采用病例对照的研究方法,共纳入MS患者57例(MS组),非MS的全身健康者26人(健康组).留取受检者静息全唾液,用放射免疫的方法检测TNF-α的质量浓度,酶联免疫吸附试验检测IL-1β的质量浓度.比较MS组和健康组唾液中细胞因子水平的差异,并分析细胞因子与牙周临床指标和MS组分数的相关关系.结果 MS组唾液中TNF-α水平[(69.30±21.01)ng/L]显著高于健康组[(57.85±15.69)ng/L],P<0.05;唾液中IL-1β的水平[(616.42±360.05)ng/L]高于健康组[(506.06±245.76)ng/L],差异无统计学意义(P>0.05).唾液中的TNF-α水平与探诊后出血呈正相关(r=0.220,P<0.05).唾液中TNF-α和IL-1β水平在MS组中随发生MS的组分数增加而增高、随牙周炎程度加重而增高,但差异均无统计学意义.结论 牙周感染可能是MS患者体内低度炎症的来源之一,唾液TNF-α、IL-1β水平可能不同程度地受全身状况和口腔感染的影响.     

不同类型牙周炎患者唾液中8-OHdG含量的比较

目的:检测不同类型牙周炎患者非刺激性全唾液中8-OHdG的含量并评价其与PD,CAL等临床指标的关系。方法:留取10名侵袭性牙周炎,10名慢性牙周炎和10名健康对照非刺激性全唾液,并记录全口PD,CAL,PLI,BI。ELLISA试剂盒检测唾液中8-OHdG含量。结果:8-OHdG含量在3组间存在统计学差异,AgP组:(3.8 ±1.0) μg/L, CP组:(2.37±0.7) μg/L,H组:(1.0±0.6) μg/L。PD,BI,PLI,CAL与8-OHdG含量存在相关关系,慢性与侵袭组中8-OHdG含量与平均CAL, CAL>5mm位点所占百分比,CAL>7 mm位点所占百分比相关。结论:唾液中8-OHdG含量可以反映牙周组织的破坏程度并与牙周炎类型存在相关关系。     

表皮生长因子及其受体在口腔黏膜上皮异常增生和口腔鳞癌中的表达

目的 探讨表皮生长因子(epidermal growth factor,EGF)及其受体(epidermal growth factor receptor,EGFR)与口腔黏膜癌变的关系.方法 ①以30例健康成年人唾液样本为对照,通过放射免疫法对12例上皮异常增生患者、40例口腔鳞癌(oral squamous cell carcinoma,OSCC)患者唾液EGF含量进行测定分析.②采用免疫组化法检测10例正常口腔黏膜,16例上皮异常增生,30例OSCC上皮组织中EGFR的表达水平.结果 ①上皮异常增生组唾液EGF含量[(5.12±4.30)μg/L]显著高于口腔鳞癌[(2.35±1.00)μg/L]和正常对照组[(2.18±1.02)μg/L](P<0.01),OSCC和正常对照组无显著性差异.②上皮异常增生组织中EGFR的表达高于正常黏膜(P<0.05).OSCC中EGFR的表达显著高于正常黏膜(P<0.01).OSCC组和上皮异常增生组织中EGFR的表达无显著性差异.结论 EGF和EGFR可能参与口腔黏膜癌变的早期阶段.     

检测汉、维族青年唾液中一氧化氮检测比较

目的:了解汉维族青年在相同条件下唾液中一氧化氮(NO)含量的差异。方法:选择健康汉族青年136名(男女各68名),维族150名(男43名女107名)年龄16～18岁,按国内报道采集唾液,由专业人员,用第三军医大学临床微生物研究室产的“一氧化氮检测试剂盒”,进行比色检测,计算唾液中NO量。结果:维族组唾液NO均值为109.760±57.038)μmol/L,汉族组唾液NO均值为(75.181±47.740)μmol/L,维族高于汉族,有高度显著性差异(P<0.01)。在性别上,维族女性唾液NO均值为(109.533±47.369)μmol/L,汉族女性为(83.647±48.759)μmol/L,存在高度显著性差异(P<0.01),两族男性之间无差异(P>0.05)。两族正常口腔者与龋、龈炎者的唾液NO均值三者间无显著性差异(P>0.05)。结论:唾液中NO含量,可因种族和女性而有差异     

涎腺疾病

细胞周期素D1在涎腺腺样囊性癌中的表达及意义；辣椒素对健康人唾液腺及泪腺分泌功能的影响；涎腺Warthin瘤的诊治;唾液腺良性肿瘤患者唾液中CA125的表达及临床意义；腮腺切除术对面神经损伤和恢复的影响。     

口腔及涎腺癌患者唾液与血清中CEA和CA-50含量的研究

目的 探讨口腔及涎腺恶性肿瘤患者唾液及血清中 CEA和 CA-50的含量。方法 应用酶联免疫和放射免疫法对80例口腔及涎腺恶性肿瘤、40例良性肿瘤、80例健康对照者进行唾液CEA和CA-50含量测定,其中60例恶性肿瘤患者同时行血清CEA和CA-50测定。结果 唾液CAE和CA-50含量在恶性肿瘤组、良性肿瘤组及健康对照组组间差异有显著性（P<0.01）。 60例恶性肿瘤血清 CEA 7例轻度升高,CA-50仅见 3例升高。结论 检测口腔及涎腺恶性肿瘤患者唾液 CEA和 CA-50含量较血清敏感,对恶性肿瘤的早期诊断有一定参考价值。     

糖尿病患者唾液葡萄糖含量分析

目的 :研究糖尿病患者唾液中葡萄糖含量的改变 ,并观察高血糖状态下唾液糖与血糖的关系。方法 :实验组为 6 0例糖尿病患者 ,对照组为 6 0例正常人。收集实验组及对照组清晨非刺激状态下自然分泌的全唾液 ,进行葡萄糖含量分析 ,同时行空腹血糖分析。结果 :糖尿病组的唾液葡萄糖含量为 ( 1.95 0±0 .179)mmol/L ,高于对照组的 ( 0 .95 3± 0 .12 4)mmol/L ,二者有显著性差异 (P <0 .0 1)。糖尿病组的唾液葡萄糖含量与血糖浓度有显著直线相关性 (P <0 .0 5 )。结论 :糖尿病患者唾液腺分泌功能发生改变 ,这可能是机体在长期高血糖状态下对唾液腺的损害 ,可视为糖尿病这一全身性代谢疾病的口腔局部表现     

原发性舍格林氏综合征患者唾液及血清白细胞介素-6测定

对原发性舍格林氏综合征患者唾液及血清白细胞介素-6含量进行测定,结合临床表现以确定患者唾液白细胞介素-6水平能否反映患者唾液腺受累情况及潜在的自身免疫紊乱。 材料及方法 原发性舍格林氏综合征患者31例,原发性胆汁性肝硬化患者15例,健康对照者14例。用2％柠檬酸刺激腮腺分泌并用Carlson-Crittenden杯收集唾液。用高灵敏度的ELISA法(酶联免疫吸附试验/测定唾液及血清白细胞介素-6含量。对舍格林氏综合征患者进行了血清免疫学、唾液功能及组织病理学检     

Evidence of the validity of a model of determinants of quality of restorative dental care

A model describing the relationship between self-reported quality of restorative dentistry and dentist characteristics for 119 Montana general dentists is presented. The best predictors formed a significant model explaining 22% of the variance of the quality measure. Results are contrasted with a previous estimation of the model for 102 Washington general practitioners. Evidence for the external validity of the model is presented.     

Experimental evidence of formation of imines in the course of reduction of hydrazones

The reduction of hydrazones is generally suggested to proceed through a reductive cleavage of the nitrogen–nitrogen bond followed by a reduction of the carbon–nitrogen bond. This sequence of reduction processes is here supported for fluorenone (V) and benzophenone (VI) hydrazones as well as by a comparison of the reduction of fluorenone and benzophenone hydrazonium ions (I,III) with corresponding imines (II,IV). Another proof of the presence of imines as intermediates is the splitting of four-electron waves of hydrazones V and VI and hydrazonium ions I and VIII into two waves at pH < 2. This has been interpreted as due to differences in slopes dE_1/2/dpH and pK_a-values of protonated hydrazine derivatives on one side and corresponding imines on the other. In this pH-range imines formed in reductions of VI and VIII are reduced in a single two-electron wave, those of I and V in two one-electron steps. Fluorenone imine (II) is sufficiently stable to allow recording of time-independent current–voltage curves between pH 6 and 11. In this pH-range the imine (II) is reduced in two one-electron steps. Benzophenone imine (IV) has been found stable between pH 4.6 and 12. At pH 4.6–8 the reduction of the imine IV takes place in a single two-electron step, at pH 8–12 in two one-electron steps. Final proof of the initial cleavage of the N–N bond is presented by comparison with the reduction of nitrones.     

不同剂型玻璃离子水门汀溶解性比较研究

目的:研究、比较不同剂型玻璃离子水门汀的溶解性和表面微观形态改变,为临床使用提供依据.方法:将3M树脂加强型玻璃离子水门汀(水粉剂型)、GC玻璃离子水门汀(水粉剂型)及GC玻璃离子水门汀(双糊剂型)分别在人工唾液中浸泡30 d,冷热循环15000次,烘干测重,比较前后质量变化,计算溶解率,并用扫描电镜观察表面微观改变.结果:不同剂型的玻璃离子水门汀溶解率由高到低分别为3M树脂加强型玻璃离子水门汀(水粉剂型)、GC玻璃离子水门汀(水粉剂型)、GC玻璃离子水门汀(双糊剂型).3种玻璃离子水门汀经浸泡溶解后,SEM扫描表面微观形态可观察到GE玻璃离子水门汀(双糊剂型)表面形态改变较少,其他2组玻璃离子水门汀表面微观改变较多.结论:双糊剂型玻璃离子水门汀理化性能及溶解率均低于传统水粉剂型,是未来临床修复治疗的的良好选择.     

Design of clinical trials of traditional therapies of periodontitis

The present paper on the design of clinical trials of periodontal therapy first addresses the issue of the etiology of periodontal disease. It is suggested that most if not all forms of destructive periodontal disease are caused by microorganisms and that there are different forms of disease with different microbial etiologies. The progressive nature of destructive periodontal disease is subsequently discussed and it is emphasized that, in a given patient, periodontal sites which show signs of inflammation and attachment loss may not over a period of several months and years show further sign of attachment loss. The present methods of assessing periodontal disease do not allow us to discriminate between potentially active and inactive sites in untreated patients. The significance and variability of indicators of periodontal disease such as bleeding on probing, probing pocket depth and probing attachment level measurements are discussed. The errors inherent in the various measurements are analyzed and suggestions are presented describing how alterations in any of the above parameters could be identified and presented in a clinical trial. Of concern for the statistical analysis of clinical data of periodontal disease is the definition of the "experimental unit". For a number of years, the "experimental unit" in periodontal trials was the patient. It is clear, however, that different sites within the same individual show different patterns of disease progression and lesion morphology and often respond differently to periodontal therapy. Statistical analyses must consequently be designed which recognize differences in site-to-site infection and lesion morphology within a common host. Until such analyses are available, the investigator should be wary of pooling data within the same individual, since such pooling may obscure meaningful alternatives which may take place in individual periodontal sites. Some goals of periodontal therapy are subsequently identified. 4 goals are discussed more in detail, namely: to establish conditions which will allow the patient to maintain a dentition without further breakdown of the periodontium; to reduce pocket depth to establish an anatomy in the dentogingival region which with proper maintainance care will prevent the re-establishment of the subgingival infection; to gain attachment as a result of treatment; to assess the effect of a certain chemotherapeutic agent on periodontal disease.     

Evaluation of efficacy of chemiluminescence for diagnosis of leukoplakia

ObjectiveLeukoplakia is the most common potentially malignant disorder preceding oral cancer. Chemiluminescence has been developed as an adjunct to conventional examination for the diagnosis of these potentially malignant disorders. This study was conducted to assess the efficacy of chemiluminescence in the diagnosis of leukoplakia and to compare the results with histopathological examination.Study designA total of 50 patients with leukoplakia were included from the outpatients attending the Department of Oral Medicine and Radiology, Dental Hospital, Bengaluru, Karnataka, India. These patients were subjected to conventional oral examination followed by chemiluminescent examination with Vizilite (Zila, Fort Collins, CO, USA) and biopsy for histopathological confirmation.ResultsThe sensitivity, specificity, positive predictive value, and negative predictive value of chemiluminescence were 93.75%, 55.56%, 78.95%, and 83.3%, respectively. The overall accuracy of chemiluminescence was 80%. A statistically significant association was observed between histopathology results and chemiluminescence results.ConclusionAlthough it is an easy, safe, minimal time consuming, and noninvasive technique, it has only adjunctive utility and it does not replace biopsy for the diagnosis of leukoplakia.     

颌骨动静脉畸形的栓塞治疗

目的：总结直接穿刺结合经血管内介入栓塞治疗颌骨动静脉静脉畸形的经验。方法：收治凳骨动静脉畸形患者6例,均进行了介入栓塞治疗。采用的栓塞材料为附凝血棉纤毛的螺圈,聚乙烯醇泡沫微粒和二氰基丙烯酸对丁酯。数字减影颈动脉造影在PHILIPSV300下完成。结果6例颌骨动静脉畸形患者中4,例急性出血得到了快速、有效控制,1例慢性渗血的右下 骨动静脉畸形患者,介入栓塞治疗,拔除松动的右下凳第一磨牙,有效地控制了出血,另1例伴局部软组织搏动性膨隆的上凳骨动静脉畸形患者,介入治疗后膨隆的搏动性得到明显改善,栓塞治疗后分别随访3－24个月,均未发现有口腔内渗血或出血。随访的X线片上,病灶区可见新骨形成。结论：局部穿刺结合经血管内介入栓塞治疗颌骨动静畸形是一种安全、有效的治疗方法。     

正畸患者切牙影像失真发生情况分析

目的研究正畸患者曲面体层片上的切牙影像失真发生情况，并分析其原因。 方法从中山大学附属口腔医院放射科影像数据库中选取500例正畸患者的曲面体层片和头影测量侧位片，所有曲面体层片均采用咬合杆投照，分别从切牙牙体影像放大、缩小、牙根变短、根尖模糊等评价指标分析上下颌切牙影像失真的发生情况，在头影测量侧位片上测量中切牙根尖-对颌切牙切缘的距离，探讨切牙影像失真发生的原因。采用SPSS 19.0统计软件对所得数据进行统计学检验。 结果500例患者中，切牙牙体影像正常者共417例，切牙牙体影像失真者共83例，影像失真发生率16.6%，其中切牙牙体影像放大17例、牙体影像缩小0例、牙根变短30例，牙根影像变短伴模糊36例。影像失真患者的根尖-切缘距离大于影像正常的患者，差异有统计学意义（F = 5 187.18，P = 0）；影像失真患者的覆盖值大于影像正常的患者，差异有统计学意义（F>477，P = 0）。 结论严重牙颌面畸形如反 、深覆盖是导致曲面体层片的切牙影像失真的主要原因之一。     

正常青年Monson球面半径的测量研究

目的测量正常青年Monson球面半径。方法选择60名(男30名,女30名)正常青年制取全口印模,应用立体摄影成像的原理与方法对Monson球面半径进行测量和统计学处理。结果Monson球面的半径平均为10.173 cm,大于理论值10.160 cm,差异有显著性(P<0.01);男、女性球面半径差异无显著性。结论本实验所得到的数据可作为全口义齿修复中记录颌位关系的一个参量。     

鼻测量法的进展

唇裂术后继发畸形是指唇裂修复术后,仍遗留或继发于手术操作和生长发育变化而表现出来的一类畸形[1]。包括唇畸形、鼻畸形和颌骨畸形。其修复较原发性唇裂修复更复杂,更灵活多变。而导致其修复复杂性的一个重要原因即是局部组织结构复杂变异和缺乏可靠的三维测量手段[2],鼻畸形     

《口腔医学》杂志2007年度有关信息的分析

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