共查询到20条相似文献,搜索用时 10 毫秒
1.
T Aoyama H Naganawa Y Muraoka H Nakamura T Aoyagi T Takeuchi Y Iitaka 《The Journal of antibiotics》1992,45(9):1391-1396
Benastatins A and B, new inhibitors of glutathione S-transferase, have been isolated from the culture broth of Streptomyces sp. MI384-DF12. By X-ray crystallography, benastatin A was determined to be 8,13-dihydro-1,7,9,11-tetrahydroxy-13-dimethyl-8-oxo-3-pentyl- benzo[a]naphthacene-2-carboxylic acid. The structure of benastatin B was elucidated by NMR studies. 相似文献
2.
Momose I Sekizawa R Hashizume H Kinoshita N Homma Y Hamada M Iinuma H Takeuchi T 《The Journal of antibiotics》2001,54(12):997-1003
Tyropeptins A and B, new proteasome inhibitors, were isolated from the culture broth of Kitasatospora sp. MK993-dF2. They were purified using ethyl acetate extraction, silica gel column chromatography, Sephadex LH-20 column chromatography and HPLC. Tyropeptin A inhibited the chymotrypsin-like (ChT-L) and trypsin-like (T-L) activities of 20S proteasome with IC50 values of 0.1 microg/ml and 1.5 microg/ml respectively, but did not inhibit the peptidylglutamyl-peptide hydrolyzing (PGPH) activity of 20S proteasome at a concentration of 100 microg/ml. The inhibitory activities of tyropeptin A were about two times as strong as that of tyropeptin B. Taxonomy of the producing strain is also described. 相似文献
3.
T Aoyagi T Aoyama F Kojima S Hattori Y Honma M Hamada T Takeuchi 《The Journal of antibiotics》1992,45(10):1587-1591
Cyclooctatin has been isolated from Streptomyces melanosporofaciens MI614-43F2 as part of a program designed to find microorganism-produced inhibitors of lysophospholipase. It was purified by chromatography on silica gel, Capcell Pak C18 (HPLC) and Sephadex LH-20 followed by solvent extraction and then isolated as a colorless powder. Cyclooctatin has the molecular formula of C20H34O3. It is competitive with the substrate, and the inhibition constant (Ki) was 4.8 x 10(-6) M. 相似文献
4.
D Komagata T Sawa Y Muraoka C Imada Y Okami T Takeuchi 《The Journal of antibiotics》1992,45(7):1117-1121
TA-3037A, a new inhibitor of glutathione S-transferase was discovered in the fermentation broth of Streptomyces sp. TA-3037. It was purified by chromatography followed by solvent extraction and then isolated as yellow needles. TA-3037A has the molecular formula of C16H11NO4. It was competitive with the substrate, and the inhibition constant (Ki) was 4.9 microM. 相似文献
5.
Momose I Iinuma H Kinoshita N Momose Y Kunimoto S Hamada M Takeuchi T 《The Journal of antibiotics》1999,52(9):781-786
New antibiotics designated decatromicins A and B were isolated from the culture broth of Actinomadura sp. MK73-NF4. They were purified by butyl acetate extraction, silica gel column chromatography, silica gel TLC and Sephadex LH-20 column chromatography. Decatromicins A and B inhibited growth of Gram-positive bacteria including methicillin-resistant Staphylococcus aureus (MRSA). 相似文献
6.
S Omura K Otoguro N Imamura H Kuga Y Takahashi R Masuma Y Tanaka H Tanaka X H Su E T You 《The Journal of antibiotics》1987,40(5):623-629
Jietacins A and B, new azoxy antibiotics, were isolated from the culture broth of a streptomycete. The antibiotics have the molecular formulae of C18H34N2O2 and C19H36N2O2, respectively. Both possess an azoxy group. They have potent activity against the pine wood nematode, Bursaphelenchus lignicolus, and are weakly active against some fungi. 相似文献
7.
Y Kishimura A Kawashima T Kagamizono M Yamagishi K Matsumoto Y Kawashima K Hanada 《The Journal of antibiotics》1992,45(6):892-898
Two new platelet aggregation inhibitors, PI-200 and PI-201 were isolated from the fermentation broth of Streptomyces sp. A7498. PI-200 and PI-201 inhibited ADP-induced aggregation of rabbit platelets with an IC50 of 3.8 x 10(-4) M and 7.1 x 10(-4) M, respectively. 相似文献
8.
Matsumoto N Momose I Umekita M Kinoshita N Chino M Iinuma H Sawa T Hamada M Takeuchi T 《The Journal of antibiotics》1998,51(12):1087-1092
Antibacterial antibiotics, diperamycin (1) was produced in the culture broth of Streptomyces griseoaurantiacus MK393-AF2. Various spectroscopic analyses of 1 suggested that 1 belonged to a member of cyclic hexadepsipeptide antibiotic. Antibiotic 1 had potent inhibitory activity against various Gram-positive bacteria including Enterococcus seriolicida and methicillin-resistant Staphylococcus aureus. 相似文献
9.
S Toda Y Obi K Numata Y Hamagishi K Tomita N Komiyama C Kotake T Furumai T Oki 《The Journal of antibiotics》1992,45(10):1573-1579
Eurystatins A and B, were isolated from the cultured broth of Streptomyces eurythermus R353-21. They showed specific and potent inhibitory activity against prolyl endopeptidase and did not show antimicrobial activity. No lethal toxicity was observed for the two compounds after ip administration in mice at 200 mg/kg. 相似文献
10.
T Aoyagi H Suda K Uotani F Kojima T Aoyama K Horiguchi M Hamada T Takeuchi 《The Journal of antibiotics》1992,45(9):1404-1408
Nagstatin, a new inhibitor of N-acetyl-beta-D-glucosaminidase (NAG-ase) was discovered in the fermentation broth of Streptomyces amakusaensis MG846-fF3. It was purified by chromatography on Dowex 50W, Avicel and Sephadex LH-20 followed by the treatment of active carbon and then isolated as colorless powder. Nagstatin has the molecular formula of C12H17N3O6. It is competitive with the substrate, and the inhibition constant (Ki) was 1.7 x 10(-8) M. 相似文献
11.
T Aoyagi S Yoshida Y Nakamura Y Shigihara M Hamada T Takeuchi 《The Journal of antibiotics》1990,43(2):143-148
Probestin has been isolated as part of a program designed to find microorganism-produced inhibitors of aminopeptidase M from Streptomyces azureus MH663-2F6. It was purified by use of column chromatography of Amberlite XAD-4, silica gel, YMC-gel, Toyopearl HW-40, YMC D-ODS-5 (HPLC) and then isolated as colorless powders. Probestin is competitive with the substrate, and the inhibition constant (Ki) of it was 1.9 x 10(-8) M. 相似文献
12.
T Aoyagi M Nagai K Ogawa F Kojima M Okada T Ikeda M Hamada T Takeuchi 《The Journal of antibiotics》1991,44(9):949-955
Poststatin, a new inhibitor of prolyl endopeptidase (PEP) was discovered in the fermentation broth of Streptomyces viridochromogenes MH534-30F3. It was purified by Diaion HP-20, Sephadex LH-20 and YMC-gel (ODS-A) column chromatography and then isolated as a colorless powder. Poststatin has the molecular formula C26H47N5O7. The IC50 value of poststatin against the PEP of partially purified porcine kidney was 0.03 microgram/ml. It has low acute toxicity. No deaths occured after iv injection of 250 mg/kg of this agent to mice. 相似文献
13.
S Miyata N Ohhata H Murai Y Masui M Ezaki S Takase M Nishikawa S Kiyoto M Okuhara M Kohsaka 《The Journal of antibiotics》1992,45(7):1029-1040
WS009 A and B novel endothelin receptor antagonists, have been isolated from the fermentation broth of Streptomyces sp. No. 89009. These antagonists were purified from the culture filtrate followed by Diaion SP-207, DEAE Toyopearl column chromatography and HPLC. WS009 A and B showed selective activity in an endothelin receptor binding assay with IC50 of 5.8 x 10(-6) M and 6.7 x 10(-7) M, respectively. On the basis of spectroscopic and chemical evidence, the structures of WS009 A and B have been established as 1 and 3, and are highly hydroxylated benz[a]anthraquinone chromophores. 相似文献
14.
Sakai Y Yoshida T Ochiai K Uosaki Y Saitoh Y Tanaka F Akiyama T Akinaga S Mizukami T 《The Journal of antibiotics》2002,55(10):855-862
Six structurally related antitumor antibiotics named GEX1 compounds were isolated from a culture broth of Streptomyces sp. GEX1A was identified as a known herbicide, herboxidiene, structurally interested by the tetrahydropyran moiety and the side chain including a conjugated diene. GEX1Q1 to approximately Q5 were determined as novel compounds related to herboxidiene. All GEX1 compounds showed cytotoxicity with IC50 values of 0.0037 to approximately 0.99 microM against human tumor cell lines in vitro, but were not active against both gram-positive and -negative bacteria. Though GEX1A/herboxidiene exhibited antitumor activity in murine tumor-planted mouse models, both GEX1Q3 and GEX1Q5 did not. 相似文献
15.
S Miyata M Hashimoto Y Masui M Ezaki S Takase M Nishikawa S Kiyoto M Okuhara M Kohsaka 《The Journal of antibiotics》1992,45(1):74-82
WS-7338 A, B, C and D, novel endothelin receptor antagonists, have been isolated from fermentation broth of Streptomyces sp. No. 7338. These antagonists were purified from the culture mycelium by extraction with acetone, followed by carbon column chromatography and HPLC. Among them, WS-7338 B showed good activity in an endothelin receptor binding assay with an IC50 of 2.7 x 10(-7) M. 相似文献
16.
N Matsumoto T Tsuchida M Maruyama N Kinoshita Y Homma H Iinuma T Sawa M Hamada T Takeuchi N Heida T Yoshioka 《The Journal of antibiotics》1999,52(3):269-275
Lactonamycin (1) was isolated from a culture broth of Streptomyces rishiriensis MJ773-88K4. Antibiotic 1 exhibited antimicrobial activities against Gram-positive bacteria including methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE). 相似文献
17.
Y Inaoka H Tamaoki S Takahashi R Enokita T Okazaki 《The Journal of antibiotics》1986,39(10):1368-1377
A soil isolate of actinomycete, strain SANK 60684, was found to produce new enkephalinase B inhibitors, propioxatins A and B. The presence of both LL- and meso-2,6-diaminopimelic acid, glycine and galactose in the cell wall assigned this strain to genus Kitasatosporia. From the morphological, cultural and physiological characteristics, this strain was determined to be Kitasatosporia setae. The Ki values of propioxatins A and B were 1.3 X 10(-8)M and 1.1 X 10(-7)M, respectively, for enkephalinase B. All other proteases examined except aminopeptidases, which were slightly inhibited, were not inhibited by these two compounds. 相似文献
18.
从链霉菌YN17707菌株发酵物中,通过活性跟踪分得三个elaiophylin类新细胞周期抑制剂及细胞凋亡诱导剂elaiophylin、11-O-monomethylelaiophylin和efomycin G。经菌种分类学研究,生产菌YN17707被鉴定为假轮枝链霉菌Streptomyces pseudoverticillus,并认证为elaiophylin类的新生产菌种。文中还探讨了该生产菌发酵生产elaiophylin类活性成分的发酵特征。 相似文献
19.
Kunimoto S Lu J Esumi H Yamazaki Y Kinoshita N Honma Y Hamada M Ohsono M Ishizuka M Takeuchi T 《The Journal of antibiotics》2003,56(12):1004-1011
Novel antibiotics named kigamicin A, B, C, D, and E were discovered from the culture broth of Amycolatopsis sp. ML630-mF1 by their selective killing activity against PANC-1 cells only under a nutrient starved condition. Under a condition of nutrient starvation, kigamicins A, B, C, and D inhibited PANC-1 cell survival at 100 times lower concentration than in normal culture. Kigamicins showed antimicrobial activity against Gram-positive bacteria including methicillin resistant Staphylococcus aureus (MRSA). Kigamicin D inhibited the growth of various mouse tumor cell lines at IC50 of about 1 microg/ml. 相似文献
20.
Novel depsipeptides, SW-163C and E were isolated from the culture broth of an actinomycete strain. The producing organism, designated as SNA15896, was identified as a member of Streptomyces from its morphological and cultural characteristics. SW-163C and E exhibited potent antitumor activities against various tumor cell lines in vitro and against murine leukemia P388 in vivo. The compounds also showed antimicrobial activities. 相似文献