薄层扫描法测定注射液中维生素B_(12)的含量

V·B_(12)注射液薄层扫描定量法准确、简便、快速。在硅胶G薄层板上点样,用甲醇与浓氨水为10:1作展开剂,展开后红色斑点用岛津CS—910型双波长薄层扫描仪,在测定波长λ_S=360nm,参考波长λ_R=400nm,反射法,Z型扫描,线性校正CH=1,线性参数SX=3,测定色斑的吸光度积分值,计算含量。本法标准差在0.02％以内,回收率为103.4％。     

HPLC测定冠心宁注射液中丹参素的含量

目的 建立冠心宁注射液中丹参素的含量测定方法.方法 采用HPLC法;Agilent Zorbax SB-C18色谱柱(SNo.:USCL021019;250 × 4.6mm,5 μm),流动相:甲醇-冰醋酸-水(25∶1∶224),检测波长280nm,柱温35℃.结果 丹参素进样量在0.04～1.25μg范围内呈良好的线性关系(r=0.99999),样品平均回收率为1 00.88%,RSD=1.55%.结论 该法操作简单、准确,可作为冠心宁注射液中丹参的水溶性成分丹参素的控制.     

薄层扫描测定复合维生素酏剂中维生素A、B_2、C和烟酰胺的含量

本文采用硅胶HF_(254)薄层,以氯仿-95％乙醇-乙酸-水(54:27:9:4)为展开剂,直接分离制剂中的维生素B_2、C和烟酰胺等水溶性维生素。用石油醚提取制剂中的脂溶性成分后,再以石油醚-乙醚(4:1)为展开剂分离维生素A、D、和E。最后于CS-910型双波长薄层扫描仪上,采用反射式锯齿形扫描,分别对维生素A、B_2、C和烟酰胺进行扫描测定。维生素Cλ_s=250nm,λ_R=400nm;烟酰胺λ_s=260nm,λ_R=400nm;维生素B_2λ_s=444nm,λ_R=700nm;维生素Aλ_s=300nm,λ_R=400nm。线性关系和精度都比较满意。回收率:维生素C 99.2％,维生素B_2100.4％,维生素A 99.9％,烟酰胺99.4％;变异系数分别为:2.1％,1.1％,1.9％和1.2%。     

HPLC测定冠心宁注射液中丹参素的含量

目的 建立冠心宁注射液中丹参素的含量测定方法.方法 采用HPLC法;Agilent Zorbax SB-C18色谱柱(SNo.:USCL021019;250 × 4.6mm,5 μm),流动相:甲醇-冰醋酸-水(25∶1∶224),检测波长280nm,柱温35℃.结果 丹参素进样量在0.04～1.25μg范围内呈良好的线性关系(r=0.99999),样品平均回收率为1 00.88%,RSD=1.55%.结论 该法操作简单、准确,可作为冠心宁注射液中丹参的水溶性成分丹参素的控制.     

薄层扫描法测定健肤液中芍药甙的含量

本文采用双波长薄层扫描法对健肤液中芍药甙含量进行测定。报道如下。1 仪器与药品CS-930型薄层扫描仪、定量毛细管、硅胶G、芍药甙、健肤液(本院提供),所用试剂均为AR级。2薄层及扫描条件 扫描条件:反射法锯齿扫描λ_R=400nm,λ_s=570nm,狭缝1.2mm×1.2mm,线性化参数S_x=3。层析条件:以硅胶G为吸附剂,用氯仿:甲醇(7:2)进行展开,5％香草醛的硫酸溶液显色,105℃烘5min。     

薄层扫描法测定心通口服液中黄芪甲苷的含量

目的:测定心通口服液中黄芪甲苷的含量.方法:选择氯仿-甲醇-水(65:30:10下层)为展开剂;10%的硫酸乙醇为显色剂;用双波长反射法锯齿形薄层扫描法进行测定,测定波长λs=515nm,参比波长λR=650nm.结果:黄芪甲苷点样量在1～5μg范围内线性关系良好,其r=0.9960.平均回收率为99.25%,RSD=2.33%(n=5).结论:本实验方法简便、快速、灵敏.     

HPLC测定冠心宁注射液中丹参素的含量

目的 建立冠心宁注射液中丹参素的含量测定方法.方法 采用HPLC法;Agilent Zorbax SB-C18色谱柱(SNo.:USCL021019;250×4.6mm,5 μM),流动相:甲醇-冰醋酸-水(25:1:224),检测波长280nm,柱温35℃.结果 丹参素进样量在0.04～1.25 μ g范围内呈良好的线性关系(r=0.99999),样品平均回收率为100.88%,RSD=1.55%.结论 该法操作简单、准确,可作为冠心宁注射液中丹参的水溶性成分丹参素的控制.     

复方丹参注射液中原儿茶醛的含量测定

目的:建立薄层层析-紫外分光光法测定复方丹参注射液中原儿茶醛的含量,控制复方丹参注射液的质量。方法:以原儿茶醛为对照品,展开剂为苯-乙酸乙酯-甲酸(8∶5∶0.8)分离得原儿茶醛,采用紫外分光光度法,检测波长281nm原儿茶醛在0.55~4.95μg/ml范围内线性关关系良好,r=0.9998,加样回收率为97.5%~102.5%,RSD为1.6%。结果:3个厂家的3个样品中原儿茶醛的含量差异无显著性(P>0.05)。结论:对复方丹参注射液的质量应作进一步调查。     

薄层扫描法测定中药马钱子制剂中士的宁和布鲁生

本文应用薄层扫描法,测定了两种中药复方制剂中士的宁(strychnine)和布鲁生(brucine)含量。样品在碱性条件下用乙醚—氯仿(3∶1)液浸泡提取,提取液于硅胶GF_(254)薄层上,以甲醇-4N氨水(9∶1)展开分离,在CS-910双波长薄层扫描仪上,λs=255nm、295nm和λ_R=400nm分别测定士的宁和布鲁生。点样量0至10.00μg与积分值呈线性关系,回收率95%以上,变异系数小于5%。     

TLC和HPLC法测定柳芽中对羟基苯乙烯

用HPLC法和TLC法对柳芽中的有效成分对羟基苯乙烯的含量进行了测定。高效液相色谱条件:色谱柱250 mm×4.5 mm(I.D.)Water's μ-Bondpak-CN 5μm;流动相:环己烷-异丙醇(95:5 V/V);检测波长UV 280 nm紫外检测器。薄层色谱法条件:固定相为硅胶G板;展开剂:苯-醋酸乙酯(2:8);以FeCl_3(5%)乙醇溶液显色后,在CS-930双波长薄层扫描仪扫描定量(λs=400nm,λ_R=650nm)。两法均获得满意结果。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.