Effect of Virgin Fatty Oil of Pistacia Lentiscus on Experimental Burn Wound's Healing in Rabbits

This study aimed to assess the efficiency of the virgin fatty oil of Pistacia lentiscus (PLVFO) for burn wounds healing. It was carried out on 6 adult male New Zealand rabbits. Four burn wounds of deep third degree were made on the back of each animal. The first was not treated and served as control (CRL group); the others were covered immediately after burning procedure by 0.5g of one of the following products: Vaseline gel (VAS group), Madecassol® cream 1% (MAD group) or 1ml of PLVFO (PLVFO group). The treatments were repeated once daily until complete healing. For four days post burns, the percentage of wound contraction was assessed. Also, the different healing times were noted. The results showed that both PLVFO and Madecassol® significantly accelerated wound healing activity compared to wounds dressed with Vaseline and the untreated wounds. However, the level of wound contraction was significantly higher and the healing time was faster in PLVFO group than those of the MAD group, VAS group and CRL group. The different epithelization periods obtained in days were respectively: 30±3.94 (PLVFO group), 33.5±3.78 (MAD group), 34.66±3.88 (VAS group) and 37.16±3.54 (CRL group). We conclude that Pistacia lentiscus virgin fatty oil promotes significantly (p< 0.05) wound contraction and reduces epithelization period in rabbit model.     

Growth factors bFGF and TGB beta accelerate the rate of wound repair in normal and in diabetic rats

The effects of basic fibroblast growth factor (bFGF) and transforming growth factor beta (TGF beta) on the rate of wound repair in both normal and streptozotocin-induced diabetic rats were investigated using two model systems of wound repair, namely incisional wounding and subcutaneous implantation of polyvinyl alcohol (PVA) sponges. Both models showed the expected wound-healing defects of the diabetic rats. Granulation tissue collected from the implanted PVA sponges showed that the diabetic rats had reduced amounts of collagen, DNA and protein present at the wound site at two time points tested (7 and 9 days post-implantation). Fresh tensile strength of the incisional wounds, a measure of the collagen organization in a wound, was reduced to 53% of normal in diabetic rats on day 7 post-wounding, and was only 29% of normal by day 21. Formalin-fixed tensile strength, a measure of collagen content of the wound, was 41% of normal on day 7, and 78% of normal by day 21, giving evidence that while the collagen concentration of the diabetic wounds approached that of normal wounds, it did not undergo the normal maturation process. A single injection of 2 micrograms of TGF beta directly into the incision three days after wounding resulted in little difference in the fresh and fixed tensile strength of diabetic wounds when tested at 7, 14 and 21 days post-wounding. Normal rats, however, responded well, resulting in a greater than 30% increase in both fresh and fixed tensile strength.(ABSTRACT TRUNCATED AT 250 WORDS)     

LED红光对糖尿病大鼠创面愈合的影响

目的探讨LED红光照射对糖尿病大鼠创面愈合的作用及相关机制。 方法将30只4周龄雄性SD大鼠按随机数字表法分为正常创面组、糖尿病创面组、红光治疗糖尿病创面组,每组10只。红光治疗糖尿病创面组及糖尿病创面组大鼠高脂饮食4周,正常创面组大鼠正常饮食。饲养4周后对红光治疗糖尿病创面组及糖尿病创面组大鼠按50 mg/kg的量腹腔注射10 mg/mL的链脲佐菌素(STZ)制作糖尿病大鼠模型,2组大鼠均造模成功。造模成功后在3组大鼠的背部两侧各制造2个1.5 cm×1.5 cm的全层皮肤缺损创面,每2 d对大鼠创面进行酒精消毒1次,红光治疗糖尿病创面组大鼠每次消毒后对创面进行LED红光照射5 min,能量密度为20 J/cm²,另外2组大鼠不进行LED红光照射。在观察第7、10、14、21天,观察红光治疗糖尿病创面组大鼠创面有无出现皮疹、红肿、水疱、烫伤等光照不良反应;肉眼观察3组大鼠创面愈合情况;统计3组大鼠创面愈合率。观察第10天从各组随机取2只大鼠,处死后取背部创面组织,固定后,进行苏木精-伊红染色观察创面新生血管情况及肉芽组织生长情况;采用免疫荧光法检测各组大鼠创面组织中CD34、血管内皮细胞生长因子(VEGF)表达情况。数据比较采用单因素方差分析和LSD-t检验。 结果观察第7、10、14、21天,红光治疗糖尿病创面组大鼠经LED红光照射后皮肤未见皮疹、红肿、水疱、烫伤等光照不良反应。在各个观察时间点,肉眼观察正常创面组及红光治疗糖尿病创面组创面愈合情况均优于糖尿病创面组,且正常创面组创面愈合情况略优于红光治疗糖尿病创面组;观察第7、10、14、21天,正常创面组的创面愈合率分别为(34.62±2.116)%、(53.83±7.92)%、(70.20±5.41)%、(95.65±2.58)%,红光治疗糖尿病创面组创面愈合率分别为(31.76±2.44)%、(50.48±4.54)%、(66.26±11.35)%、(93.96±2.80)%,糖尿病创面组创面愈合率分别为(23.67±4.18)%、(42.71±3.40)%、(53.77±7.74)%、(84.07±4.43)%,3组比较差异均有统计学意义(F＝34.69、10.35、10.32、34.40,P<0.05);观察第7、10、14、21天,正常创面组及红光治疗糖尿病创面组创面愈合率始终高于糖尿病创面组,差异均有统计学意义(P<0.05);观察第7、10天,正常创面组创面愈合率高于红光治疗糖尿病创面组,差异均有统计学意义(t＝2.80、3.26,P<0.05),观察第14、21天,正常创面组创面愈合率仍高于红光治疗糖尿病创面组,但差异均无统计学意义(t＝1.16、1.40,P>0.05)。观察第10天,创面组织苏木精-伊红染色显示,正常创面组内含大量新生毛细血管,肉芽组织内胶原及细胞排列紧密有序;红光治疗糖尿病创面组见较多新生毛细血管,肉芽组织内胶原及细胞较多,但少于正常创面组;而糖尿病创面组新生血管最少,肉芽组织内细胞及胶原稀疏。免疫荧光法检测创面组织中CD34、VEGF表达情况可见,正常创面组CD34、VEGF表达高于红光治疗糖尿病创面组,而红光治疗糖尿病创面组表达高于糖尿病创面组。 结论LED红光可促进糖尿病大鼠创面组织中CD34、VEGF表达,促进血管新生,进而促进创面愈合。     

Macroscopic aspects of wound healing (contraction and epithelialisation) after topical administration of allicin in dogs

Macroscopical aspects of second-intention healing of full-thickness excisional wounds were studied in five normal male mixed-breed dogs. Test wounds were treated topically with allicin 0.5% in methyl cellulose gel, and control wounds were treated with methyl cellulose gel only. Wound treatment started 24 h after wounding. The wounds were evaluated over a 4-week period. At days 0, 3, 7, 10, 14, 17, 21, 24 and 28, digital photographs were taken of all wounds. Rulers were held vertically and horizontally close to the wound as a reference. The area of epithelialisation and granulation tissue were measured for each wound using Scion Image software. Percentage wound contraction, epithelialisation and healing were calculated for each wound. Initially, all wound areas increased in size. After the initial enlargement, wound areas decreased rapidly in size between days 7 and 17 in both the test and control groups. Epithelialisation was first noticed at day 3 in control and day 5 in the test wounds. No significant differences were observed in the percentage of wound contraction, epithelialisation and healing between the test and control wounds (P> 0.05).     

Ethanolic extract of sharah, Plectranthus aegyptiacus, enhances healing of skin wound in rats

Sharah, Plectranthus aegyptiacus (Forssk.) C. Chr. is a common native plant in the Taif region of Saudi Arabia. An ethanolic extract of freeze dried sharah leaves was added as 10% (w/w) to an ointment base of beeswax and sesame oil. The resultant ointment was examined as a potential enhancer of wound healing. Excision wounds in the nape region of the skin were induced in sixty albino Wistar rats. Animals were allocated in 4 groups (n = 15) and kept individually in clean cages. The first group served as negative untreated controls without medication; the second group was treated with ointment base (vehicle); the third group represented the positive control and was treated with a reference ointment and the fourth one served as the experimental group and received the test plant extract (as ointment). Animal groups received the respective medications for 14 successive days. Wounds were measured and photographed every 3 days till the end of the experiment (day 21) in order to determine the wound closure rate (WCR). Specimens from wounds and surrounding skin were collected from sacrificed animals for histological and molecular studies. Both morphometric (based on WCR) and histological findings showed that the healing in animals treated with the sharah plant extract was better than those in control group or vehicle-treated group and was similar to that in the group that received the reference ointment. Moreover, the molecular findings concerning the expression levels of hepatocyte growth factor (HGF) and its receptor (c-Met) displayed a reasonable healing enhancing effect of the plant extract with the expression levels of both being higher in the extract-treated group than in the control group.     

Wound healing of 6.45-microm free electron laser skin incisions with heat-conducting templates

We have previously shown a reduction in lateral thermal damage with acute studies of skin incisions made in vitro using heat-conducting templates. Here we examined the wound-healing response to laser incisions with heat-conducting templates and explored the use of an optically transparent template with the free electron laser (FEL) at 6.45 microm. First we evaluated the effects of a sapphire heat-conducting template on the lateral thermal damage of FEL incisions using in vitro human skin samples. Next we compared wound tensile strength and histological scoring of the healing of incisions created on the dorsal pelts of live rats with the FEL utilizing metal and sapphire heat-conducting templates and scalpel incisions. The animals were euthanized and the wounds were analyzed at postoperative days 7, 14, and 21. The depth and lateral thermal damage of FEL incisions on in vitro human skin were significantly reduced with the sapphire heat-conducting template. Nonstatistically significant differences in wound tensile strengths and histological scoring of wound healing were noted at days 7 and 14. By day 21, all of the incisions appeared similar. When the data from days 7 and 14 were combined, statistically significant differences were found for each of the templates (except the histological evaluation with the aluminum template) and the scalpel compared with laser incisions made without using a template. The use of metal or sapphire heat-conducting templates reduced the wound-healing delay of laser incisions seen at postoperative days 7 and 14.     

基质细胞衍生因子-1α联合Integra支架修复全层皮肤缺损创面的实验研究

观察基质细胞衍生因子-1α(SDF-1α)联合Integra支架促进全层皮肤缺损创面愈合的作用,并探讨其作用机制。 方法选取6～8周龄的雄性健康C57小鼠30只,在其脊柱两侧对称部位分别作直径1 cm的圆形全层皮肤缺损创面,采用真皮支架Integra作为创面覆盖物。将30只小鼠背部左右对称创面按随机数字表法分为2组：SDF-1α组和对照组,各30个创面。SDF-1α组经皮下往创面内注射SDF-α,对照组皮下注射磷酸盐缓冲溶液。术后第3、6、12、18和24天观察创面的愈合时间和愈合创面收缩情况,并留取创面及周围组织标本行苏木精-伊红染色和免疫组织化学染色观察浸润细胞、肉芽组织厚度及血管化情况。对数据进行t检验。 结果(1) SDF-1α组创面完全愈合时间为(15.7±1.6)d,明显短于对照组的(19.6±1.8)d,差异有统计学意义(t＝3.967,P<0.05);(2)SDF-1α组术后第3、6、12天愈合创面收缩率分别为(7.3±3.3)%、(14.7±8.4)%、(27.6±6.3)%,与对照组[(8.3±2.5)%、(17.5±6.4)%、(31.2±16.5)%]比较,差异均无统计学意义(t＝0.6427、0.262、0.208,P值均大于0.05);SDF-1α组术后第18、24天愈合创面收缩率为(36.6±6.7)%、(58.2±7.1)%,小于对照组[(67.6±10.7)%、(81.1±8.3)%],差异均有统计学意义(t＝2.463、2.094,P值均小于0.05);(3)创面肉芽组织术后第3天SDF-1α组浸润细胞数目为(181.7±28.8)个/视野,与对照组[(190.8±33.4)个/视野]比较,差异无统计学意义(t＝ 4.08, P<0.05);术后第6天SDF-1α组浸润细胞数目[(382.2±43.4)个/视野],与对照组[((478.2±38.8)个/视野]比较,差异无统计学意义(t＝ 6.20,P<0.05);(4)肉芽组织的厚度术后第6、12天SDF-1α组创面的肉芽组织厚度为(255.8±41.8)、(387.6±36.8)μm,均小于对照组(407.3±43.4)、(490.2±49.4)μm],差异均有统计学意义(t＝4.08、6.159,P值均小于0.05);(5)SDF-1α组术后第24天愈合创面与正常皮肤更为相似,对照组瘢痕明显,表皮薄;(6)SDF-1α组术后第12天创面肉芽组织中CD3、CD31阳性细胞的密度稍高于对照组,肉芽组织的血管密度明显高于对照组。 结论局部使用SDF-1α可以促进全层皮肤缺损创面肉芽组织血管化,改善创面修复的效果。     

Effects of topical allicin on second-intention wound healing in dogs (histological aspects)

Alllicin is one of the pharmacologically active garlic sulfur compounds that have antimicrobial (antibacterial, antiviral, antifungal and antiparasitic) and vasodilating effects. Five normal, male, mixed-breed dogs were selected to investigate the effects of allicin (5 mg/ml in methyl cellulose gel) as a topical treatment for full-thickness, excisional wounds. The dogs were approximately 3 years old. The histological aspects of second-intention wound healing were studied. Eight full-thickness skin wounds (20×20 mm) were created on the back of each dog. On days 0, 7, 14 and 21, each dog received two wounds, symmetrically, and were assigned to one of two groups: control (methyl cellulose gel) or test (allicin 5 mg/ml methyl cellulose gel). Wounds were treated once daily for a week. Left-side wounds were treated with allicfin (test group) and right-side wounds were treated with methylcellulose gel (control group). At day 28 (4 weeks) after initial wounding, biopsies were taken from wounds for histological examination. The density of inflammatory cells in the center of the day 7 wounds was significantly lower in test group (P=0.041), but the density of fibrocytes and fibroblasts in the center of day 7 wounds was significantly higher in the test group (P=0.042). No significant differences were observed in the amount of collagen and fibrin between the test and control wounds (P>0.05).     

南极大磷虾胰蛋白酶可促进创面的愈合

背景：胰蛋白酶已被证实具有较好的清创、促进创伤愈合作用。 目的：探讨南极大磷虾胰蛋白酶对创面的修复效果。 方法：采用全层皮肤缺损法制成新西兰大白兔背部创伤模型,将创面分为4组,分别给予从南极大磷虾中提取的粗酶液或纯化的胰蛋白酶、木瓜蛋白酶和生理盐水,每天早晚两次给药,直至伤口愈合。 结果与结论：给药5,10和15 d测量伤口愈合面积,从南极大磷虾中提取的粗酶和纯化胰蛋白酶均具有良好的促进愈合效果,但粗酶的效果更好,其各时段残余创面面积小于生理盐水组和木瓜蛋白酶组(P < 0.05);皮肤羟脯氨酸含量测定结果显示粗酶能显著促进创伤组织中胶原的合成和积累;组织学切片观察显示粗酶组愈合效果最佳。证明南极大磷虾胰蛋白酶能显著促进新西兰大白兔创口的愈合,粗酶具有最优效果。     

Low-Level Laser Therapy Facilitates Superficial Wound Healing in Humans: A Triple-Blind, Sham-Controlled Study

OBJECTIVE: Low-level laser therapy (LLLT) has been promoted for its beneficial effects on tissue healing and pain relief. However, according to the results of in vivo studies, the effectiveness of this modality varies. Our purpose was to assess the putative effects of LLLT on healing using an experimental wound model. DESIGN AND SETTING: We used a randomized, triple-blind, placebo-controlled design with 2 within-subjects factors (wound and time) and 1 between-subjects factor (group). Data were collected in the laboratory setting. SUBJECTS: Twenty-two healthy subjects (age = 21 +/- 1 years, height = 175.6 +/- 9.8 cm, mass = 76.2 +/- 14.2 kg). MEASUREMENTS: Two standardized 1.27-cm(2) abrasions were induced on the anterior forearm. After wound cleaning, standardized digital photos were recorded. Each subject then received LLLT (8 J/cm(2); treatment time = 2 minutes, 5 seconds; pulse rate = 700 Hz) to 1 of the 2 randomly chosen wounds from either a laser or a sham 46-diode cluster head. Subjects reported back to the laboratory on days 2 to 10 to be photographed and receive LLLT and on day 20 to be photographed. Data were analyzed for wound contraction (area), color changes (chromatic red), and luminance. RESULTS: A group x wound x time interaction was detected for area measurements. At days 6, 8, and 10, follow-up testing revealed that the laser group had smaller wounds than the sham group for both the treated and the untreated wounds (P < .05). No group x wound x time differences were detected for chromatic red or luminance. CONCLUSIONS: The LLLT resulted in enhanced healing as measured by wound contraction. The untreated wounds in subjects treated with LLLT contracted more than the wounds in the sham group, so LLLT may produce an indirect healing effect on surrounding tissues. These data indicate that LLLT is an effective modality to facilitate wound contraction of partial-thickness wounds.     

重组人内抑素对兔耳创面瘢痕组织血管内皮生长因子、转化生长因子-β_1和碱性成纤维细胞生长因子表达的影响

目的观察重组人内抑素(rh Endostatin)对兔耳创面瘢痕增生及血管内皮生长因子(VEGF)、转化生长因子β1(TGF-β1)和碱性成纤维细胞生长因子(b FGF)表达的影响,探讨rh Endostatin抑制瘢痕增生的分子机制。方法健康新西兰大耳白兔20只,均分为正常对照组、模型组、生理盐水(NS)对照组、rh Endostatin(5 g/L)治疗组和醋酸曲安奈德(TA,40 g/L)对照组;除正常对照组外其余各组均进行增生性瘢痕动物模型的复制,在兔耳腹侧面制作1cm×1cm大小创面。术后第28天,rh Endostatin治疗组瘢痕皮内注射相应浓度rh Endostatin(100μl),NS对照组注射等量生理盐水,隔日1次,共6次;TA对照组瘢痕块内注入相应浓度曲安奈德(100μl),每周1次,共2次;模型组术后不接受处理。术后第47天摄片并收集瘢痕及正常皮肤标本,应用免疫组织化学方法检测VEGF、TGF-β1和b FGF的表达。结果形态学观察结果显示,术后第47天rh Endostatin治疗组瘢痕皮肤色泽变浅,变软变平,体积减小,与模型组和NS对照组比较有明显差异;免疫组织化学检测结果可见,与模型组和NS对照组相比,rh Endostatin治疗组VEGF和TGF-β1蛋白表达减少,b FGF表达增加,差异均具有统计学意义(P0.01)。结论 rh Endostatin能有效抑制兔耳创面瘢痕增生,其机制可能与rh Endostatin影响VEGF、TGF-β1和b FGF蛋白的表达有关。     

Study of Wound-Healing Activity of Bioregulators Isolated from Eye Tissues and Bovine Serum in the Model of Experimental Corneal Injury in Rabbits In Vivo

We compared wound-healing activity of bioregulators isolated from cattle cornea, serum, and retinal pigment epithelium on in vivo model of experimental corneal injury in rabbits. Bioregulators were instilled into the eye as solutions at a concentration corresponding to 10^-12 mg protein/ml. The animals were sacrificed on day 21 after injury and the corneas were examined histologically. The best wound-healing effect was produced by bioregulators isolated from the cornea and serum and instilled successively into rabbit eyes with an interval of 15-20 min twice a day: multicellular epithelium was observed in the wound, and slight inflammation, in the stroma.     

Effect of collagen cross-linking inhibition by local application of beta-aminopropionitrile fumarate on wound contraction and healing (macroscopic study)

Wound contraction is a major component of second-intention wound healing. The mechanism of this process is not completely understood. Two theories have been described for the mechanism of the wound contraction. To evaluate the collagen cross-linking inhibition on wound contraction, the present study was carried out. Macroscopical aspects of second-intention healing of full-thickness, excisional wounds were studied in five normal male mixed-breed dogs. Under general anesthesia, two full-thickness skin wounds (20 × 20 mm) were created on the back of each dog symmetrically. Left-side wounds (test group) and right-side wounds (control group) were treated topically with beta-aminopropionitrile fumarate 5 mg/ml in methyl cellulose gel and methyl cellulose gel, respectively. Wounds were treated starting at 24 h after wounding and continued for ten successive days. The wounds were evaluated over a 4-week period. At the days 0, 3, 7, 10, 14, 17, 21, 24, and 28, digital photographs were taken of all wounds. Rulers were held vertically and horizontally close to the wound as a reference. The area of the epithelialization and granulation tissue were measured for each wound using Scion Image software. Percent of the wound contraction, epithelialization, and healing were calculated for each wounds. Wound contraction, epithelialization, and healing were significantly decreased in the wounds treated by beta-aminopropionitrile fumarate (P < 0.05). Our data demonstrated that the collagen and collagen cross-linking play a key role in the wound contraction and healing during the first 10 days of the wound healing.     

糖尿病模型大鼠烫伤创面皮肤组织P物质和Bcl-2表达与龙血竭的干预*

背景：研究证明龙血竭有促进创伤愈合、抗血小板聚集、抗炎镇痛、抗菌抗氧化等生物活性。 目的：验证龙血竭在糖尿病大鼠烫伤皮肤组织愈合过程中对P物质和Bcl-2表达的影响。 方法：Wistar大鼠112只,随机分为4组。龙血竭组、磺胺嘧啶银(SD-Ag)组、糖尿病对照组和正常对照组。其中前3组为糖尿病深Ⅱ度烫伤模型,正常对照组为正常深Ⅱ度烫伤模型。 结果与结论：烫伤后第7天开始创面愈合率龙血竭组、正常对照组高于SD-Ag组和糖尿病对照组(P < 0.05),第21天龙血竭组创面愈合率明显高于SD-Ag组和糖尿病对照组(P < 0.05),正常对照组最高。烫伤后第15天正常对照组P物质阳性表达水平最高,峰值显著高于其他3组,以后逐步减弱。龙血竭组、SD-Ag组和糖尿病对照组于烫伤后第21天阳性表达最强,且龙血竭组表达明显高于SD-Ag组和糖尿病对照组(P < 0.05)。烫伤后第3天开始正常对照组Bcl-2阳性表达水平明显高于其他3组;于第15天各组阳性表达水平皆达到峰值,龙血竭组最高,明显高于SD-Ag组和糖尿病对照组(P < 0.05),与正常对照组比较差异无显著性意义(P > 0.05)。说明龙血竭能通过参与调控P物质和Bcl-2的表达,有效地促进糖尿病烫伤创面的愈合。     

纳米银－猪脱细胞真皮基质敷料治疗浅Ⅱ度烧伤创面的临床观察

目的观察纳米银－猪脱细胞真皮基质敷料在临床上治疗浅Ⅱ度烧伤创面的临床疗效。 方法2014年1月到2015年12月,选取北京军区总医院烧伤整形科收治的浅Ⅱ度烧伤患者90例,按入院顺序依次编码,采用随机排列数字表法将90例患者分为纳米银敷料组、猪脱细胞真皮基质敷料组及纳米银－猪脱细胞真皮基质敷料组,每组30例。患者入院当天,拍照计算创面面积,并用咽拭子取创面分泌物作细菌培养,行创面清创术,分别在创面上敷以纳米银敷料、猪脱细胞真皮基质敷料以及纳米银－猪脱细胞真皮基质敷料。于治疗后第5天,用咽拭子取创面分泌物作细菌培养;采用痛觉评分标准,通过询问与观察患者换药时的痛觉情况,评估患者换药时痛觉评分。于治疗后第7天,拍照计算创面面积,计算创面愈合率。记录创面最终愈合时间。数据比较采用单因素方差分析、χ²检验及SNK－q检验。 结果纳米银敷料组、猪脱细胞真皮基质敷料组及纳米银－猪脱细胞真皮基质敷料组在治疗后第5天创面细菌培养阳性数结果分别为2例(6.6%)、9例(30.0%)、1例(3.3%),3组结果比较,差异有统计学意义(χ²＝10.962,P＝0.004);纳米银－猪脱细胞真皮基质敷料组的细菌培养阳性数结果显著优于猪脱细胞真皮基质敷料组,差异有统计学意义(χ²＝7.680,P＝0.006)。纳米银敷料组、猪脱细胞真皮基质敷料组及纳米银－猪脱细胞真皮基质敷料组治疗后第5天的痛觉评分[(8.6±0.5)、(6.6±0.8)、(0.6±1.3)分],治疗后第7天的创面愈合率[(61.67±18.22)%、(86.77±15.32)%、(99.80±0.56)%],创面愈合时间[(11.5±1.3)、(10.3±0.7)、(7.3±0.7)d],组间差异均有统计学意义差异(F＝201.7、19.9、55.7,P值均小于0.05);纳米银－猪脱细胞真皮基质敷料组治疗后第5天的痛觉评分显著低于其余两组,差异均有统计学意义(P值均小于0.05);治疗后第7天的创面愈合率明显优于其余两组,差异均有统计学意义(P值均小于0.05);创面愈合时间显著短于其余两组,比较差异均有统计学意义(P值均小于0.05)。 结论纳米银－猪脱细胞真皮基质敷料具有抗感染、促进创面愈合及减轻换药痛觉的作用。     

选择性去细胞猪皮在深Ⅱ度烧伤创面中的应用

目的将选择性去细胞猪皮用于深Ⅱ度烧伤创面早期包扎,观察其创面保护和促愈效果。方法 41例烧伤患者,男性29例,女性12例,年龄18～53岁,平均（31.4±9.7）岁,烧伤面积5%～39%。伤后2～4d,2%创面清创后以选择性去细胞猪皮覆盖,其余创面常规换药,以同体同侧或对侧创面作为对照,隔日换药,观察创面愈合情况,计算伤后2周创面愈合率,记录创面愈合时间。结果选择性去细胞猪皮与创面基底贴敷良好,创周无红肿等炎症反应表现,14～21d逐渐干燥、脱落,创面愈合。伤后2周创面愈合率（82.34±20.13）%,对照组为（76.21±22.52）%。创面平均愈合时间（17.8±4.3）d,对照组为（21.2±5.6）d。两者相比差异均有统计学意义。结论选择性去细胞猪皮对于清创后的深Ⅱ度烧伤创面有较好的保护和促愈作用,效果优于常规换药方法。     

上肢骨折术后伤口负压引流管与普通引流管的比较

目的 探讨上肢骨折术后伤口负压引流管与普通引流管对伤口引流量的影响.方法 选取2018年8月至2019年8月的上肢闭合性骨折经过手术治疗患者44例,以电脑随机程序分为负压组(伤口负压引流)24例和普通组(普通伤口引流)20例.观察术后ld、2d及拔出引流管时伤口引流量及引流管放置时间;比较术前、术后血红蛋白含量及红细胞...     

肉芽组织下注射微粒皮浆对大鼠创伤性慢性创面愈合的影响

目的研究肉芽组织下注射微粒皮浆对大鼠创伤性慢性创面愈合的作用。 方法选取60只SD雄性大鼠,于背部制作大小为3.0 cm×3.0 cm的创面,并将钢丝圈缝于创面内缘。按照随机数字表法将大鼠分为3组,分别为一般创面组、慢性创面组和微粒皮浆组,每组各20只。一般创面组背部造成创面后给予抗感染治疗并常规换药;慢性创面组背部形成创面后给予抗感染治疗、常规换药,并连续7 d肌内注射氢化可的松干预形成慢性创面;微粒皮浆组背部形成创面后给予抗感染治疗、常规换药,连续7 d肌内注射氢化可的松干预形成慢性创面,取大鼠右大腿外侧皮肤制备微粒皮浆注射于肉芽组织下。造模完成次日开始观察创面情况,定为观察第1天。观察第7、14、21、28天各组创面愈合情况,并计算创面愈合率;留取观察第14天的肉芽组织进行苏木精-伊红染色以及CD31免疫组织化学染色,观察苏木精-伊红染色下创面新生毛细血管分布情况及CD31免疫组织化学染色下CD31表达情况与微血管密度。对数据行单因素方差分析和LSD-t检验。 结果观察第14天,一般创面组创面明显缩小,慢性创面组皮肤爬伸不明显,微粒皮浆组创面大部分愈合;观察第28天,一般创面组剩余部分残留创面,慢性创面组创面愈合不明显,微粒皮浆组创面基本愈合。观察第14、21、28天,一般创面组愈合率分别为(51.09±0.94)%、(75.43±0.92)%、(86.51±0.57)%,慢性创面组创面愈合率分别为(20.30±0.95)%、(35.59±1.18)%、(45.82±1.35)%,微粒皮浆组创面愈合率分别为(39.00±0.86)%、(64.62±0.15)%、(91.25±0.87)%,比较差异均有统计学意义(F＝1 993.60、6 475.02、9 984.47,P值均小于0.05);观察第14天,慢性创面组创面愈合率分别与一般创面组、微粒皮浆组比较,差异均有统计学意义(t＝89.90、50.93,P值均小于0.05);观察第21天,慢性创面组创面愈合率分别与一般创面组、微粒皮浆组比较,差异均有统计学意义(t＝117.90、116.10,P值均小于0.05);观察第28天,慢性创面组创面愈合率分别与一般创面组、微粒皮浆组比较,差异均有统计学意义(t＝86.43、94.29,P值均小于0.05)。观察第14天,创面苏木精-伊红染色观察,一般创面组可见少许新生毛细血管,慢性创面组未见明显新生毛细血管,微粒皮浆组其间有大量新生毛细血管。观察第14天,创面CD31免疫组织化学染色观察(CD31阳性表达呈棕黄色),一般创面组棕黄色的颗粒散在分布,慢性创面组棕黄色颗粒稀疏分布,微粒皮浆组可见大量棕黄色的颗粒分布。观察第14天,创面微血管密度比较,一般创面组、慢性创面组、微粒皮浆组微血管密度分别为(49.20±17.96)、(37.32±9.57)、(64.93±20.29)个/视野,比较差异有统计学意义(F＝34.09,P<0.05);慢性创面组创面微血管密度分别与一般创面组、微粒皮浆组比较,差异有统计学意义(t＝3.23、11.50,P值均小于0.05)。 结论微粒皮浆肉芽组织下注射可促进大鼠创伤性慢性创面血管增生,其创面愈合率明显升高,创面愈合时间缩短。     

Effect of synthetic peptide thrombin receptor agonist encapsulated in microparticles based on lactic and glycolic acid copolymer on healing of experimental skin wounds in mice

PAR1 peptide thrombin receptor agonist (PAR1-AP) was encapsulated in microcorpuscles based on lactic and glycolic acid copolymer. The desorption profile of the preparation was studied in vitro and its wound-healing effects were studied on a model of cut skin wound in mice. The study showed that 90% PAR1-AP was desorbed over 6 h, but the peptide was detected in eluates from the microparticle surface after 23 h. The desorbed peptide retained its physiological activity and was capable of activating PAR1 receptors on human platelets. The study of the dynamics of experimental skin wound healing in mice showed lower number of macrophages in the wounds treated with PAR1-AP microparticles compared to the control (open wounds and wounds covered with microparticles) and higher number of fibroblasts on day 3 of tissue reparation. Hence, PAR1-AP desorbed from microparticles shortened the inflammation phase in the wound. On day 7 the best healing parameters were also observed in wounds treated with PAR1-AP microparticles, which attests to shortening of the proliferation phase and acceleration of wound healing.Translated from Byulleten Eksperimentalnoi Biologii i Meditsiny, Vol. 138, No. 11, pp. 523–526, November, 2004     

Effect of Peptide Bioregulator on Healing of Excision Wounds in Old Animals

Aging is associated with reduction of protein synthesis in cells, which leads to deceleration of proliferative processes in various tissues. Recovery of damaged skin sites was stimulated with a peptide bioregulator chondrolux. This agent is based on an extract from calf cartilage and bone tissue. Its effect on healing of excision wounds was studied in old rabbits. Morphological analysis of the wound surface during various periods of healing was carried out by electron microscopy. The results indicate that chondrolux application to the wound surface stimulated and optimized the reparative process. Active development of granulation tissue was observed as early as on day 14 after wound infliction (vs. days 21-28 in control animals). Acceleration of wound healing was paralleled by an increase in functional activities of fibroblast organelles.