国产羟基磷灰石义眼座植入术的临床观察

目的：评价国产羟基磷灰石义眼座植入的临床疗效。方法：对50例患者行羟基磷灰石义眼座植入术。其中I期植入34例，Ⅱ植入16例，术后随访3-42个月。结果：术后48例外观效果满意，并发症主要有结膜裂开、义眼台暴露及自体巩膜溶解。结论：国产羟基磷灰石同样是理想的眶内植入材料。     

羟基磷灰石义眼台植入108例效果观察

目的 对羟基磷灰石义眼台植入术进行观察,评价义眼台植入的临床效果.方法 108例羟基磷灰石义眼台植入术患者中Ⅰ期植入87例,Ⅱ期植入21例.其中采用无包裹羟基磷灰石义眼台直接植入10例;自体巩膜壳内植入79例;异体巩膜壳内植入19例.随访3月～3年.结果 108例术后早期均有不同程度眼睑及结膜水肿,但随加压包扎去除逐渐消退.4例羟基磷灰石义眼座暴露,1例不影响效果,未处理,2例经再次充分分离筋膜组织,无张力缝合后愈合,1例暴露直径＞10 mm,经2次穹隆结膜松解重新缝合后愈合.Ⅱ期植入3例出现结膜囊狭窄,经成形后恢复;1例眼外肌无法充分暴露,术后义眼活动部分受限.其余107例义眼活动良好,配戴仿真义眼片后眼外观满意.结论 羟基磷灰石义眼座植入术式操作简单,美容效果佳.Ⅰ期较Ⅱ期植入义眼活动度及外观好,尽量行Ⅰ期植入,但术前手术方式选择尤其重要,Ⅰ期植入应尽量采取保留自体巩膜的方式,Ⅱ期植入时异体巩膜壳内植入优于无包裹义眼台直接植入.     

羟基磷灰石义眼台植入时机对手术效果的影响

目的:观察眼球破裂眼内容剜出与羟基磷灰石义眼台植入手术时机对术后效果的影响.方法:对46例眼球破裂患者病例资料进行回顾性分析,其中28例Ⅰ期手术行眼内容剜出与羟基磷灰石义眼台植入,18例先行眼球伤口缝合术,Ⅱ期手术行眼内容剜出与羟基磷灰石义眼台植入,观察术后情况.结果:近期效果Ⅱ期手术反应严重,远期效果无差异.结论:羟基磷灰石义眼台植入手术时机对近期效果有明显影响,远期效果无影响.     

羟基磷灰石眼座Ⅱ期植入临床观察

目的：观察羟基磷灰石眼座Ⅱ期植入术的效果。方法：对19例Ⅱ期义眼台植入术并发症分析。结果：术后半年，义眼片活动良好52．7％，中等36．8％，不良10．5％；并发症15．78％。结论：Ⅱ期植入羟基磷灰石只要掌握手术要领，即可避免并发症，获得良好效果。     

基层医院开展羟基磷灰石义眼台植入临床观察

目的探讨羟基磷灰石义眼台植入的临床应用及手术效果。方法采用自体巩膜覆盖义眼台或者直接缝合眼外肌的的形式,对13例患者植入羟基磷灰石义眼台。结果所有羟基磷灰石义眼台植入后,患者外观满意,没有排斥反应,术后安装薄义眼片后外观仿真,活动良好达到预期效果。结论羟基磷灰石义眼台植入是眼窝充填术中的一种较理想的植入体及手术方法。     

羟基磷灰石义眼台植入术20例临床分析

目的：探讨羟基磷灰石义眼台植入术的临床应用及其疗效。方法：观察20例20眼巩膜壳后肌锥间隙内羟基磷灰石义眼台植入术患者,Ⅰ期植入者9例,Ⅱ期植入者11例。结果：随访时间2～37个月,平均17个月,均获得较满意的效果。结论：巩膜壳后羟基磷灰石义眼台植入术,义眼台固定于肌锥间隙内,并保留了眼外肌与巩膜原有的解剖关系,损伤小,术后反应轻,并发症少。     

羟基磷灰石义眼台Ⅱ期植入术临床观察

目的:观察和探讨羟基磷灰石义眼台Ⅱ期植入的手术技巧和疗效.方法:对35例(35眼)眼球缺如患者进行羟基磷灰石义眼台Ⅱ期植入术.结果:术后结膜一期愈合31例,结膜切口裂开1例,出现义眼台暴露5例.随访3个月～2年,眼外肌正常者义眼活动度良好,眼外肌萎缩者义眼活动度稍差.多数患者对义眼的外形和活动度感到满意.结论:羟基磷灰石义眼台Ⅱ期植入术为较可靠的眼部成形术.     

羟基磷灰石义眼座植入术的临床观察

目的：观察眼球摘除或眼内容摘除术后羟基磷灰石义眼座眼眶内植入的术后效果。方法：对19例眼球摘除或眼内容摘除术后的患者采用羟基磷灰石义眼座行Ⅰ期或Ⅱ期眶内植入，观察其疗效。结果：随访2个月至24个月，除2例球结膜裂开，义眼座暴露以外，其余病例均取得良好效果，外观满意，义眼片动度良好。结论：羟基磷灰石义眼座眼眶内植入疗效满意，美容效果理想，是目前无眼球畸形的良好眼眶内充填材料。     

羟基磷灰石义眼台眶内植入术临床观察

目的观察巩膜包裹羟基磷灰石义眼台植入法的临床效果。方法用自体巩膜包裹义眼台Ⅰ期眶内植入20例（20眼）。结果随访3个月-1年，义眼活动均好，配戴仿真义眼片后眼外观满意。无1例发生义眼台感染、排斥。结论羟基磷灰石术中行自体巩膜包裹Ⅰ期眶内植入的义眼台活动度及外观较好。     

完整巩膜壳后羟基磷灰石义眼台植入的临床观察

目的观察自体完整巩膜壳后羟基磷灰石(hydnroxypatite,HA)义眼台植入法疗效。方法眶内植入HA义眼台共52眼,=1\*ROMANⅠ期植入48眼,=2\*ROMANⅡ期植入4眼,均为自体巩膜壳后羟基磷灰石义眼台植入。随访3个月～13年。结果无义眼台暴露。结论 HA义眼台植入术后主要并发症是义眼台暴露,自体巩膜壳后羟基磷灰石义眼台植入无发生这种并发症的几率。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.