异叶梁王茶中三萜皂苷的研究

从异叶粱王茶树皮的醇提取物中分离得到两种新的三萜皂苷化学物，异叶梁王茶苷Ⅶ［ｌ］和异叶梁王茶苷Ⅷ［２］；以及一种已知化合物梁王茶苷Ⅱ［３］。经光谱和化学分析，分别鉴定为３－Ｏ－β－（２’，４’－Ｏ－二乙酰基）－Ｄ－吡喃木糖－３β－羟齐墩果－１２－烯－２８，２９－双羧酸－２８－Ｏ－［α－Ｌ－吡喃鼠李糖（１－４）－β－Ｄ－吡喃葡萄糖（１－６）－β－Ｄ－吡喃葡萄糖］酯苷［１］；３－Ｏ－β－（３’－Ｏ－乙酰基）－Ｄ－吡喃木糖－３β－羟齐墩果－１２－烯－２８，２９－双羧酸－２８－Ｏ－［α－Ｌ－吡喃鼠李糖（１－４）－β－Ｄ－吡喃葡萄糖（１－６）－β－Ｄ－吡喃葡萄糖］酯苷［２］。     

异叶梁王茶化学成分的研究

从异叶梁王茶[Nothopanax davidii(Franch)Harms]树皮中分离得到两种五环三萜皂甙,经化学和光谱(IR,¹HNMR,¹³CNMR,¹H-¹H COSY,¹H-¹³C COSY,NOESY,MS)分析,分别鉴定为3-O-α-(3′,4′-O-二乙酰基)-L-吡喃阿拉伯糖-β-羟齐墩果-12-烯-28,29-双羧酸-28-O-[α-L-吡喃鼠李糖(1→6)-β-D-吡喃葡萄糖(1→4)-β-D-吡喃葡萄糖]酯甙(Ⅱ)和3-O-α-(3′-O-乙酰基)-L-吡喃阿拉伯糖-3β-羟齐墩果-12-烯-28,29-双羧酸甙(Ⅱ),前者命名为异叶梁王茶甙Ⅰ,后者命名为异叶梁王茶甙Ⅱ。这两个皂甙均系新化合物。     

异叶梁王茶化学成分的研究

从异叶梁王茶甲醇提取物中分离出八种五环三萜皂甙单体A_4,A_7,A_9,D_1,S_2,S_3,S_5、S_6。经IR、MS、1D-NMR、2D-NMR以及化学方法确定了前六种五环三萜皂甙的结构。这六种三萜皂甙均为首次从植物界中分离出的新化合物。     

异叶梁王茶甙Ⅲ和Ⅳ的结构

继前报后,又从异叶梁王茶[Nothopanax davidii(France)Harms]树皮中分离得到两种五环三萜皂甙。经化学和波谱(IR,¹HNMR,~(13)CHMR,¹H-¹HCOSY,¹³C-¹H COSY,MS)分析,分别鉴定为3-O-α-(2’,4’-O-二乙酰基)-L-吡喃阿拉伯糖-3β-羟基齐墩果-12-烯-28,29-双羧酸-28-O-[α-L-吡喃鼠李糖(1-4)-β-D-吡喃葡萄糖(1—6)-β-D-吡喃葡萄糖]酯甙(Ⅲ),命名为异叶梁王茶甙Ⅲ和3-O-β-(2-O-乙酰基)-D-吡喃木糖-3β-羟齐墩果-12-烯-28,29-双羧酸甙(Ⅳ),命名为异叶梁王茶甙Ⅳ。这两种皂甙均系首次从植物中分出的新化合物。     

异叶梁王茶甙Ⅲ和Ⅳ的结构

继前报后,又从异叶梁王茶[Nothopanax davidii(France)Harms]树皮中分离得到两种五环三萜皂甙。经化学和波谱(IR,~1HNMR,~(13)CHMR,~1H-~1HCOSY,~(13)C-~1H COSY,MS)分析,分别鉴定为3-O-α-(2’,4’-O-二乙酰基)-L-吡喃阿拉伯糖-3β-羟基齐墩果-12-烯-28,29-双羧酸-28-O-[α-L-吡喃鼠李糖(1-4)-β-D-吡喃葡萄糖(1—6)-β-D-吡喃葡萄糖]酯甙(Ⅲ),命名为异叶梁王茶甙Ⅲ和3-O-β-(2-O-乙酰基)-D-吡喃木糖-3β-羟齐墩果-12-烯-28,29-双羧酸甙(Ⅳ),命名为异叶梁王茶甙Ⅳ。这两种皂甙均系首次从植物中分出的新化合物。     

高效液相色谱法测定异叶爬山虎中异槲皮苷的含量

目的 建立高效液相色谱法(HPLC)测定异叶爬山虎中异槲皮苷含量的方法,并测定广西不同地区的异叶爬山虎中异槲皮苷的含量.方法 采用Inersil-C18色谱柱(4.6 mm×25 mm,5μm),流动相为甲醇-0.05%磷酸溶液(46:54),流速1 mL·min-1,检测波长258 nm,柱温25℃,进样量10μL,分析时间32 min.结果 异槲皮苷的进样量在20~200μg范围内,与峰面积呈良好的线性关系,相关系数r=0.9999(n=6);异槲皮苷的平均回收率为98.53%(n=6),RSD为0.79%.结论 该法操作简便,重复性好,可用于测定异叶爬山虎中异槲皮苷含量.     

丝瓜叶的三萜皂甙，丝瓜皂甙Q

从丝瓜（Luffa cylindrica(L.)M.Roem.)叶中分得一种新的五环三萜皂苷和芹菜素，用光谱学和化学方法确定该皂苷的结构为21β－羟基齐墩果酸－28－O－β－D－吡喃葡萄糖苷，命名为丝瓜皂苷Q。     

太白Song木根皮的三萜皂甙成分研究

从太原song木（Sralia taibaiensis Z.Z.Wang et H.C.Zheng)根皮中首次分离得到四个三萜皂甙，根据理化性质和光谱数据，分别鉴定为齐墩果酸－3－O－[β－D－吡喃木糖1－→2）][β-D-吡喃葡萄糖（1→3)]-β-D-吡喃葡萄糖醛酸甙（1），tarasaponin V(2),3-O-{[β-D-吡喃木糖（1→2）][β-D-吡喃葡萄糖（1→3）]-β-D吡喃葡萄糖醛酸乙酯}－齐墩果酸－28－O－β－D－吡喃葡萄糖甙（3）和3－O－{[β=D 吡喃木糖（1→2)][β－D吡喃葡萄糖（1→）]-β-D吡喃葡萄糖醛酸丁酯}－齐墩果酸28－O－β－D－吡喃葡萄糖甙（4），1为新天然产物，3和4为新化合物，分别命名为太白Song木皂甙VI（taibaienoside VI),太白Song木皂甙Ⅶ（taibaienosideⅦ)和太白Song木皂甙Ⅷ（taibaienosideⅧ)。     

黑枣叶茶VC人体利用率的研究

9名青年男性,在预备膳和基本无Vc的实验膳基础上,给予Vc,使机体Vc达饱和状态后,分别每日给予纯Vc、黑枣叶茶浸提液,并分别测定24小时尿中Vc排出量,计算出排出率。根据Melnick的方法求得黑枣叶茶Vc的人体利用率平均约为73％。结合各期血浆Vc测定结果,预备期、茶Vc期及纯Vc期分别为0.28/dl、0.72/dl及1.01mg/dl。表明每日摄入黑枣叶茶Vc60mg能维持血浆Vc浓度于较充裕水平。     

异叶三宝木枝条的化学成分研究

目的 研究异叶三宝木 (Trigonostemon heterophyllus Merr.)枝条乙醇提取物的化学成分。方法 采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱和 RP-18柱色谱进行分离纯化, 并根据波谱数据进行结构鉴定。 结果 从异叶三宝木中分离得到8个化合物,经波谱分析鉴定其结构为原儿茶酸甲酯 (1)、3-羟基-4-甲氧基苯甲酸甲酯 (2)、反式对羟基桂皮酸甲酯 (3)、4-羟基-3-甲氧基苯甲酸 (4)、jacareubin (5)、(+)-lyoniresinol (6)、表百麦根苷 (7)和 β-谷甾醇 (8)。结论 以上化合物均为首次从该属植物中分离得到。滤纸片琼脂扩散法表明化合物 5 对于金黄色葡萄球菌 (SA)和耐甲氧西林金黄色葡萄球菌 (MRSA)有抑制作用。     

Triterpenoid Saponins from the Root Bark of Araliataibaiensis

从太白木（ＡｒａｌｉａｔａｉｂａｉｅｎｓｉｓＺ．Ｚ．ＷａｎｇｅｔＨ．Ｃ．Ｚｈｅｎｇ）根皮中首次分离得到四个三萜皂甙，根据理化性质和光谱数据，分别鉴定为齐墩果酸３Ｏ［βＤ吡喃木糖（１→２）］［βＤ吡喃葡萄糖（１→３）］βＤ吡喃葡萄糖醛酸甙（１），ｔａｒａｓａｐｏｎｉｎⅤ（２），３Ｏ｛［βＤ吡喃木糖（１→２）］［βＤ吡喃葡萄糖（１→３）］βＤ吡喃葡萄糖醛酸乙酯｝齐墩果酸２８ＯβＤ吡喃葡萄糖甙（３）和３Ｏ｛［βＤ吡喃木糖（１→２）］［βＤ吡喃葡萄糖（１→３）］βＤ吡喃葡萄糖醛酸丁酯｝齐墩果酸２８ＯβＤ吡喃葡萄糖甙（４）。１为新天然产物，３和４为新化合物，分别命名为太白木皂甙Ⅵ（ｔａｉｂａｉｅｎｏｓｉｄｅⅥ）、太白木皂甙Ⅶ（ｔａｉｂａｉｅｎｏｓｉｄｅⅦ）和太白木皂甙Ⅷ（ｔａｉｂａｉｅｎｏｓｉｄｅⅧ）。     

Triterpenoid Saponins from Primula denticulata

Two triterpene glycosides denticin ( 1) and denticulatin ( 2) have been isolated from the whole plant of PRIMULA DENTICULATA Sm. and their structures were established by spectral and chemical studies as 3beta-{ O-beta- D-glucopyranosyl-(1-->4)-[ O-beta- D-xylopyranosyl-(1-->2)]- O-(beta- D-glucopyranosyl-(1-->4)-[ O-beta- D-glucopyranosyl-(1-->2)]-alpha- L-arabinopyranosyloxy}-16alpha-hydroxy-13beta, 28-epoxyolean-30-al and 3beta-{ O-beta- D-glucopyranosyl-(1-->2)- O-beta- D-glucopyranosyl-(1-->4)-[ O-beta- D-xylopyranosyl-(1-->2)]- O-beta- D-glucopyranosyl-(1-->4)-[ O-beta- D-glucopyranosyl-(1-->2)]-alpha-L-arabinopyranosyloxy}-16alpha-hydroxy-13beta,28-epoxyolean-30-al, respectively.     

Triterpenoid Saponins from Anemone flaccida

From the rhizome of ANEMONE FLACCIDA Fr. Schmidt, used in Chinese folk medicine, four saponins of oleanolic acid-A,B,C,D were isolated. By means of spectroscopy and chemical transformation, saponins A, B, and D were identified as 3- O-[alpha- L-rhamnopyranosyl-(1-->2)-alpha- L-arabinopyrasyl]-oleanolic acid, 3- O-[alpha- L-rhamnopyranosyl-(1-->2)-beta- D-xylopyranosyl]-oleanolic acid, and 3- O-[alpha- L-rhamnopyranosyl-(1-->2)-beta- D-glucopyranosyl]-oleanolic acid. These three saponins are known compounds but isolated from ANEMONE plants for the first time. Saponin C was elucidated as 3- O-[beta- D-glucopyranosyl-(1-->2)-beta- D-xylopyranosy1]-oleanolic acid, which has not been reported in the literature before, and is named as flaccidoside I.     

Triterpenoid Saponins from the Leaves of Ilex Kudincha

Abstract

Eight new triterpenoid saponins, kudinosides I-P along with three known saponins were isolated from the leaves of Ilex kudincha. The aglycone moiety of kudinosides I-K was determined as a new triterpene (kudinolic acid), a fore-genin for the lactonic triterpene. The structures of all saponins were established on the basis of spectroscopic and chemical evidence. The biogenesis of the triterpene with lactonic moiety was briefly discussed.     

中药墨旱莲中的三萜皂苷

从中药墨旱莲Eclipta prostrataL．中分离得到5个三萜皂苷,根据理化性质和光谱数据,分别鉴定为eclalbasaponins Ⅱ(1),Ⅰ(2),Ⅲ(3),3-O[β-D-吡喃葡糖(1→2)-β-D-吡喃葡糖]-16R-乙氧基-齐墩果酸-28-O-β-D-吡喃葡糖苷(4)和3-O[β-D-吡喃葡糖(1→2)-β-D-吡喃葡糖]-刺囊酸-28-Oβ-D-吡喃葡糖苷(5)。化合物4和5为新化合物,分别命名为eclalbasaponins XI和eclalbasaponins XII。化合物1和5具有诱导稻瘟霉菌丝变形活性。     

Three New Triterpenoid Saponins from the Seeds of Vaccaria segetalis

Abstract

Three new triterpenoid saponins, named segetoside G (1), H (2) and I (3), have been isolated from the seeds of Vaccaria segetails. On the basis of chemical reaction and spectral data, their structures have been established as: 28-O-β-D-xylopyranosyl-(1 → 4)-α-L-rhamnopyranosyl-(1 → 2)-[α-L-arabinofuranosyl (1 → 3)]-β-D-(4-O-acetyl)-fucopyranosyl-gypsogenin-3-O-β-D-gal-actopyranosyl-(1 → 2)-β-D-(6-O-butyl ester)-glucuronopyranoside (1), 28-O-β-D-xylopyranosyl-(1 → 4)-α-L-rhamnopyranosyl-(1 → 2)-[α-L-(5-O-acetyl)-arabinofuranosyl-(1 → 3)]-β-D-(4-O-acetyl)-fucopyranosyl-gypsogenin-3-O-β-D-galactopyranosyl-(1 → 2)-β-D-glucuronopyranoside (2) and 28-O-β-D-xylopyranosyl-(1 → 4)-α-L-rhamnopyranosyl-(1 → 2)-[α-L-(5-O-acetyl)-arabinofuranosyl-(1 → 3)]-β-D-(4-O-acetyl)-fucopyranosyl-quillaic acid-3-O-β-D-galactopyranosyl-(1 → 2)-β-D-glucuronopyranoside (3).