共查询到19条相似文献,搜索用时 46 毫秒
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新一代大环内酯类抗生素地红霉素 总被引:7,自引:0,他引:7
新一代大环内酯类抗生素地红霉素是红霉素的衍生物。其抗菌活性与红霉素类似,具有比红霉素优异的药代动力学特性,对酸稳定,临床上对各种感染性疾病均有满意的疗效。本文综述了地红霉素的合成工艺、体内外抗菌作用,药代动力学特性和临床应用。 相似文献
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地红霉素的组织分布 总被引:3,自引:0,他引:3
朱汝锦 《国外医药(抗生素分册)》1995,16(4):269-272
通常要求的抗生素体内活性,其必须在感染部位能迅速达到有效而持久浓度。由于根据血浆动力学数据不能对特定组织抗生素浓度作出精确预测,因此必须进行组织分布研究.现已对数种大环内酯抗生素进行了此类研究,其中地红霉素(dirithromycin)是一个红霉素(口恶)嗪衍生物,其抗菌谱与红霉素相似,并具有组织亲和性高及半衰期长的特点。Bergogne—Brzine综合描述了地红霉素在组织中的分布。 相似文献
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地红霉素的抗菌活性,药动学特性和临床疗效 总被引:8,自引:0,他引:8
孙涛 《国外医药(抗生素分册)》1998,19(4):297-301
地红霉素(drithromycin)是一个14元口服大环内酯类抗生素,是红霉环胺和一个脂肪醛的缩合产物.由于其体外抗菌谱和抗菌活性与红霉素大体相似,而且在体外与体内一样容易被水解为有生物活性的代谢产物红霉环胺,因此,对该化合物活性的体外研究实际上至少可以部分地测定该化合物的活性.与红霉素相比,在软组织中地红霉素的药动学特性有所改善,其消除半衰期长,且与其它由细胞色素P450系统代谢的药物相互作用少. 相似文献
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目的建立HPLC法测定地红霉素在其肠溶片中的含量。方法采用Hypersil BDS C18柱(200mm×4.6mm,5μm);流动相为乙腈-磷酸盐缓冲液(取1.41g磷酸二氢钾与6.91g磷酸氢二钾,加水1000mL溶解后,过滤,即得)-甲醇(44:37:19);检测波长为205nm;柱温40℃。结果地红霉素在0.8~4.7mg·mL^-1浓度与峰面积呈良好的线性关系(r=0.9999),平均回收率为99.5%,RSD为0.31%(n=9)。结论方法简便、快速、灵敏、准确。 相似文献
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Organic concentrates of water of the rivers Rhine and Meuse and a control lake were tested for mulagenic activity with the Ames Salmonella/microsome assay at 3-mth intervals for more than 1 yr. The river samples were concentrated by adsorption on XAD (103-fold) followed by elution with DMSO.Using strain TA 98, all Rhine water samples except one were found to contain both direct and indirect mutagens. No significant mutagenic activity was detected in the lake samples and most of the river Meuse samples. None of the samples were shown to be mutagenic when tested with strains TA 100 or TA 1535.On examining one Rhine location more frequently, the mutagenic activity was found to be persistent and to vary about 5- to 6-fold during one year.Finally, liver homogenates of bream (Abramis brama) from these waters were compared with the standard rat liver S-9 with regard to their ability to activate the indirect mutagens present in the water concentrates. Compared with the rat liver homogenates, the liver homogenates of Rhine fish were found to be equally active and those of Meuse fish somewhat less. No metabolic activation was observed with liver homogenates of the lake fish. 相似文献
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反相高效液相色谱法测定地红霉素的含量 总被引:2,自引:0,他引:2
报道反相高效液相色谱法测定地红霉素含量的方法。采用ODS柱 (15 0× 4 6mm) ,以磷酸三乙胺缓冲液 乙腈 甲醇 (35∶45∶2 0 )为流动相 ,使用紫外检测器 ,于 2 15nm波长处测定。线性范围为 1 0~ 9 0g·L-1(r =0 9999) ,RSD =0 9% (n =9)。检测限为 5 0× 10 -2 mg。本法可对地红霉素中的 (9)S 红霉胺、16 (R) 地红霉素、16 (S) 地红霉素的等组分进行分离测定 ,具有快速、简便、灵敏、准确的特点 相似文献
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《Regulatory toxicology and pharmacology : RTP》2015,73(3):506-513
In the neotropical savannah, Astronium species are used in popular medicine to treat allergies, inflammation, diarrhea and ulcers. Given that natural products are promising starting points for the discovery of novel potentially therapeutic agents, the aim of the present study was to investigate the mutagenic and antimutagenic activities of hydroalcoholic extracts of Astronium spp. The mutagenicity was determined by the Ames test on Salmonella typhimurium strains TA98, TA97a, TA100 and TA102. The antimutagenicity was tested against the direct-acting and indirect-acting mutagens. The results showed that none of the extracts induce any increase in the number of revertants, demonstrating the absence of mutagenic activity. On the other hand, the results on the antimutagenic potential showed a moderate inhibitory effect against NPD and a strong protective effect against B[a]P and AFB1. This study highlights the importance of screening species of Astronium for new medicinal compounds. The promising results obtained open up new avenues for further study and provide a better understanding the mechanisms by which these species act in protecting DNA from damage. However, further pharmacological and toxicological investigations of crude extracts of Astronium spp., as well as of its secondary metabolites, are necessary to determine the mechanism(s) of action to guarantee their safer and more effective application to human health. 相似文献
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目的 研究白眉蝮蛇 (Agkistrodonhalysussuriensis)毒中精氨酸酯酶的致突变作用。 方法 用鼠伤寒沙门细菌营养缺陷型突变株TA97,TA98,TA10 0和TA10 2 ,采用平皿掺入法进行Ames试验 ,将实验分为加和不加代谢激活系统S92组平行试验。精氨酸酯酶设 6个浓度 :10 .0× 10 -3 ,5 .0× 10 -3 ,2 .5× 10 -3 ,1.2 5× 10 -3 ,0 .6 2 5× 10 -3 和 0 .312× 10 -3 U/mL。结果 加和不加代谢激活系统S9两种条件下 ,精氨酸酯酶不诱发鼠伤寒沙门细菌营养缺陷型突株的回复突变。Ames试验结果为阴性。结论 从致突变角度考虑 ,精氨酸酯酶在高于“蝮蛇清栓酶”(主要成分为精氨酸酯酶 )临床治疗剂量约 10 0 0倍的条件下仍然较安全。 相似文献
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目的:建立浊度法测定地红霉素效价的方法,及效价测定的最佳条件并探讨地红霉素含量测定的最佳方法。方法:浊度法以金黄色葡萄球菌为实验菌,加菌量为1.5%~3%(V/V),(37±1)℃培养4~6h 测定。同时以高效液相色谱法、管碟法对样品及对照品进行测定。结果:地红霉素浊度法抗生素线性浓度范围为1.0~10.0μg·mL~(-1),回归方程为 A=384.448C 588.816,r=0.9998;一剂量、二剂量的平均回收率分别为102.5%(n=5)、101.7%(n=6),口内 RSD 分别为1.45%,0.94%;日间 RSD 分别为1.97%,1.12%。结论:浊度法灵敏,快速,可作为地红霉素效价测定法。 相似文献
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地红霉素肠溶微丸胶囊的生物等效性 总被引:2,自引:0,他引:2
目的:建立准确、灵敏的HPLC-MS测定人血浆中红霉素胺浓度的方法,研究地红霉素肠溶微丸胶囊健康志愿者生物等效性。方法:20名健康志愿者采用两制剂双周期自身对照交叉试验设计,单剂量口服地红霉素试验制剂和参比制剂各500mg,用液相色谱-质谱法测定血浆地红霉素代谢物红霉素胺的浓度,并计算两制剂的主要药动学参数及相对生物利用度。结果:红霉素胺的浓度与吸收峰面积比值在4.5-720μg·L^-1范围内线性关系良好,线性回归方程为C=0.014A-0.0214,r=0.9998(n=5),最低定量限为4.5μg·L^-1。低、中、高3种浓度的方法回收率为98.89%,99.78%,98.65%,平均提取回收率为70.55%,70.50%,71.52%,日内、日间RSD均小于10%。单剂量口服500mg的地红霉素试验制剂和参比制剂的主要药动学参数AUG0→96(梯形法)分别为(3025.6±690.9),(2862.8±672.1)μg·h·L^-1,AUG0-∞(梯形法)分别为(3679.0±888.1),(3500.9±813.2)μg·h·L^-1;Cmax(实测)分别为(390.4±81.2)ng·mL^-1和(396.0±63.2)μg·L^-1;tmax(实测)为(3.3±0.4),(3.35±0.29)h。受试制剂的相对生物利用度为(107.6±20.2)%。结论:该方法简单,准确度高,灵敏度好,可用于地红霉素人血药浓度测定。统计分析结果表明,两种制剂的主要药动学参数之间无明显差异;双单侧t检验结果表明,两种制剂具有生物等效性。 相似文献
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Jian Yan Michelle Bishop M. Yvonne Jones Fumiya Watanabe Alexandru S. Biris Penelope Rice Tong Zhou Tao Chen 《Journal of applied toxicology : JAT》2012,32(11):934-943
Titanium dioxide nanoparticles (TiO2‐NPs) are being used increasingly for various industrial and consumer products, including cosmetics and sunscreens because of their photoactive properties. Therefore, the toxicity of TiO2‐NPs needs to be thoroughly understood. In the present study, the genotoxicity of 10nm uncoated sphere TiO2‐NPs with an anatase crystalline structure, which has been well characterized in a previous study, was assessed using the Salmonella reverse mutation assay (Ames test) and the single‐cell gel electrophoresis (Comet) assay. For the Ames test, Salmonella strains TA102, TA100, TA1537, TA98 and TA1535 were preincubated with eight different concentrations of the TiO2‐NPs for 4 h at 37 °C, ranging from 0 to 4915.2 µg per plate. No mutation induction was found. Analyses with transmission electron microscopy (TEM) and energy‐dispersive X‐ray spectroscopy (EDS) showed that the TiO2‐NPs were not able to enter the bacterial cell. For the Comet assay, TK6 cells were treated with 0–200 µg ml–1 TiO2‐NPs for 24 h at 37 °C to detect DNA damage. Although the TK6 cells did take up TiO2‐NPs, no significant induction of DNA breakage or oxidative DNA damage was observed in the treated cells using the standard alkaline Comet assay and the endonuclease III (EndoIII) and human 8‐hydroxyguanine DNA‐glycosylase (hOGG1)‐modified Comet assay, respectively. These results suggest that TiO2‐NPs are not genotoxic under the conditions of the Ames test and Comet assay. Published 2012. This article is a US Government work and is in the public domain in the USA. 相似文献