肝硬变合并原发性腹膜炎患者血清和腹水IL-6，IL-2，TNFα的变化

本文对４６例肝炎肝硬变患者血清及腹水中ＩＬ６，ＩＬ２，ＴＮＦα水平进行了检测，旨在探讨联合检测这三种细胞因子对肝硬变合并自发性腹膜炎（ＳＢＰ）的诊断价值．１材料和方法１．１材料４６例均系慢性乙型肝炎肝硬变合并腹水患者，诊断符合１９９５年（北京）全...     

PCⅢ，HA，LN内科不同疾病中的表现

ＰＣⅢ，ＨＡ，ＬＮ内科不同疾病中的表现胡滨韩清锡陈寿权潘景业林秀英徐海燕Ｓｕｂｊｅｃｔｈｅａｄｉｎｇｓｐｒｏｃｏｌａｇｅｎ／ｂｌｏｏｄ；ｈｙａｌｕｒｏｎｉｃａｃｉｄ／ｂｌｏｏｄ；ｌａｍｉｎｉｎ／ｂｌｏｏｄ；ｌｉｖｅｒｃｉｒｒｈｏｓｉｓ／ｂｌｏｏｄ；...     

男性胃癌血清性激素水平检测及其意义

男性胃癌血清性激素水平检测及其意义王耀芝１王锦荣２李艳丽１阮翘２付迎辉２１河南省中医院郑州市４５０００２２河南医科大学第一附属医院核医学室４５００５３ＳｕｂｊｅｃｔｈｅａｄｉｎｇｓＳｔｏｍａｃｈｎｅｏｐｌａｓｍｓＳｉｘｈｅｒｍｏｎｅｓ／ａｎａｌｙ...     

中药复方 慎柔养真汤 中血管活性肠肽的探讨

目的中药复方慎柔养真汤中是否含有血管活性肠肽（ＶＩＰ），以探索该药治疗消化系统疾病的作用机理．方法用放射免疫分析药盒，对中药复方慎柔养真汤煎剂，２０例正常人和２０例脾阴虚证患者血浆中ＶＩＰ的含量进行检测．结果最大结合率Ｂ０／Ｔ＝５３２９０％；非特异性结合率Ｎ／Ｔ＝１１７０％；拟合曲线方程Ｙ＝０８１９８３＋０４４３１９Ｘ０２８９２７Ｘ２，拟合度Ｒ２＝０９９０；中药中ＶＩＰ＝１０６６ｎｇ／Ｌ±２０ｎｇ／Ｌ，与正常人相比较无显著性差异，但有升高趋势；正常人血浆中ＶＩＰ＝９０１６ｎｇ／Ｌ±１５ｎｇ／Ｌ；脾阴虚患者血浆中ＶＩＰ＝６３２５ｎｇ／Ｌ±１１ｎｇ／Ｌ，与正常人相比较有显著性差异（Ｐ＜００５）．结论中药复方慎柔养真汤煎剂中有ＶＩＰ或ＶＩＰ前体复合物，其ＶＩＰ水平略高于正常人血浆，而脾阴虚患者血浆中ＶＩＰ水平低于正常人．     

胰腺癌酶学诊断价值

胰腺癌酶学诊断价值侯振江１张宗英２郭金英３Ｓｕｂｊｅｃｔｈｅａｄｉｎｇｓｐａｎｃｒｅａｔｉｃｎｅｏｐｌａｓｍｓ／ｅｎｚｙｍｏｌｏｇｙ；ｒｉｂｏｎｕｃｌｅａｓｅｓ／ａｎａｌｙｓｉｓ；ｔｒｙｐｓｉｎ／ａｎａｌｙｓｉｓ；ｐｈｏｓｐｈｏｌｉｐａｓｅｓＡ／...     

血清层粘蛋白和Ⅳ型胶原鉴别良恶性肝肿瘤的价值

目的研究血清层粘蛋白（ＬＮ）和Ⅳ型胶原（ＣⅣ）在良恶性肝肿瘤鉴别中的价值．方法肝恶性肿瘤患者４６例，良性肝占位患者３１例，其他恶性肿瘤患者３８例和健康献血员４０例．采用放射免疫法分别测定空腹血清ＬＮ和ＣⅣ．用ＳＮ６９５型智能放射测量仪进行测量．结果肝恶性肿瘤组血清ＬＮ水平（２４７μｇ／Ｌ±９２μｇ／Ｌ）显著高于正常对照组（９５μｇ／Ｌ±２１μｇ／Ｌ，Ｐ＜００１）与良性肝肿瘤组（１０６μｇ／Ｌ±５１μｇ／Ｌ，Ｐ＜００１）；肝恶性肿瘤组血清ＣⅣ水平（４１２μｇ／Ｌ±６４μｇ／Ｌ）显著高于良性肝占位组（９０μｇ／Ｌ±２０μｇ／Ｌ，Ｐ＜００１）、其他恶性肿瘤组（９９μｇ／Ｌ±３７μｇ／Ｌ，Ｐ＜００１）和正常对照组（８４μｇ／Ｌ±１７μｇ／Ｌ，Ｐ＜００１）．血清ＬＮ和ＣⅣ对良恶性肝肿瘤鉴别诊断的准确率分别为８１８％和８９６％．对肝癌的敏感性分别为８６９％和９１３％．结论血清ＬＮ和ＣⅣ对良恶性肝肿瘤的鉴别诊断具有一定价值     

缓下寒湿，补火升清法治疗五更泻69例

１对象和方法１．１对象全部病例符合《中医病证诊断疗效标准》［１］的泄泻之肾阳亏虚型（五更泻）诊断标准．其中男４３例，女２６例；年龄２０岁～５７岁，平均４０岁；病程１ａ～２３ａ；均为我院消化科门诊患者，无其他系统严重合并症．１．２方法冷积丸１粒／次，３...     

胃癌细胞DNA含量肿瘤相关抗原及其异质性

目的探讨胃癌异质性标志物之间的关系．方法应用显微分光光度计和抗ＳＳＥＡ１，抗胃癌单抗ＭＧ７的免疫组织化学染色对３５例胃癌和１０例正常胃粘膜进行了ＤＮＡ含量和ＳＳＥＡ１，ＭＧ７抗原表达的研究．结果正常胃粘膜细胞和管状腺癌，低分化腺癌，粘液腺癌，印戒细胞癌及未分化癌的ＤＮＡ相对含量分别为１１６０±０２０７，２１７６±０７０３，２２１４±０６６３，２２０６±０６０２，１１９６±０２７９和１６６５±０４９１，其中正常胃粘膜９５８％细胞为２Ｃ细胞，而胃癌常见＞８Ｃ，＞１２Ｃ细胞，在直方图上分布弥散；同一类型的不同病例间，同一病例切片的不同部位呈明显的异质性，但印戒细胞癌的ＤＮＡ含量接近正常．大部分胃癌标本中可检出ＳＳＥＡ１和ＭＧ７Ａｇ，但分布及染色程度呈明显的不均质性．ＳＳＥＡ１，ＭＧ７Ａｇ的表达与ＤＮＡ含量无明显关系．结论ＤＮＡ含量和ＳＳＥＡ１，ＭＧ７的表达是互相独立的，均与肿瘤的行为有关．     

慢性胃病T细胞亚群、一氧化氮、氨基己糖含量与Hp感染的研究

目的探讨慢性胃病患者T细胞亚群、一氧化氮(NO)、氨基己糖含量变化与Hp感染的关系.方法分别对30例慢性胃炎(CG)、32例消化性溃疡合并慢性胃炎(PU+CG)与20例正常对照分别进行T细胞亚群、NO、氨基己糖含量检测.结果 CG患者外周血CD+3, CD+4, CD+8, CD+4/CD+8(60.7%±2.4%, 35.8%±2.5%, 25.2%±2.4%,1.43±0.15)分别较正常对照组(68.3%±3.9%, 43.0%±3.8%,26.4%±1.7%,1.64±0.18)明显降低(P＜0.05～0.01);其Hp+组CD+3, CD+4(59.7%±2.5%,34.8%±2.6%)较Hp-组(62.2%±0.9%, 36.9%±2.0%)为低(P＜0.01,0.05);重度CG患者71%有Hp感染,其CD+3, CD+4, CD+4/ CD+8(59.5%±2.1%,35.4%±2.7%,1.40±0.13)分别均较轻中度患者(62.3%±1.7%,37.4%±1.8%,1.50±0.13)明显减低(P＜0.05～0.01). CG和PU+CG患者Hp感染率分别为60.0%和75.0%,其氨基己糖含量(mg/g,40±6,39±8)较Hp-组(45±8,51±7)明显减低(P＜0.05,0.01);重度CG患者氨基己糖含量(mg/g,38±4)明显低于轻中度患者(47±8,P＜0.01). CG和PU+CG组NO含量(μg/L,2514±364, 2824±673)均明显高于正常对照组(μg/L,2227±214, P＜0.05,0.01);其Hp+组NO含量(μg/L,2671±258,3071±398)均明显高于Hp-组(μg/L,2282±387,2579±668,P＜0.01,0.05);重度CG和PU伴重度CG患者NO含量(μg/L,2656±251, 2880±802)分别较轻中度患者明显增高(2328±413, 2403±284,P＜0.05,0.05).结论 CG尤其是重症患者存在细胞免疫功能紊乱. CG,PU伴Hp感染患者胃粘膜氨基己糖含量随感染发生、发展呈进行性减低,血浆NO含量也随Hp感染、病变程度加重呈进行性升高.     

Influence of BOL on hyaluronic acid, laminin and hyperplasia in hepatofibrotic rats

AIM:To study the anti-hapatofibrosis mechanism of Bie JiaJian oral liquid(BOL).METHODS:The model was induced by subcutaneousinjection of CCl_4.BOL was administered and the change ofserum hyaluronic acid(HA)and laminin(LN)was observedand the degeneration of liver cells and the degree of fibrehyperplasia analyzed.Changes of ultra micro-structure inliver cells were observed in some samples.RESULTS:HA was reduced in both the groupa with low andhigh dosage of BOL.which showed a remarkable differenceas compared with that of the model group(low dosagegroup:376.15μg/L±35.48μg/L vs 806.07μg/L±98.49μg/L,P<0.05;high dosage group:340.14μg/L±30.18μg/L vs806.07μg/L±98.49μg/L,P<0,05).The LN content of lowand high dosage group of BOL was lower than that of modelgroup(low dosage group:71.99μg/L±8.15μg/L vs 133.94μg/L±14.45μg/L,P<0.01;high dosage group:71.68μg/L±11.62μg/L vs 133.94μg/L±14.45μg/L,P<0.01)andcolchicine group(low dosage group:71.99μg/L±8.15μg/Lvs 118.28μg/L±16.13μg/L,P<0.05;high dosage group:71.68μg/L±11.62μg/L vs 118.28μg/L±16.13μg/L,P<0.05).Examined by Rldit,BOL could reduce thedegeneration and necrosis of liver cells(X~2=11.99P<0.05),the degree of fibre hyperplasia(X~2=13.24P<0.05)and the pathological change of ultra micro-structure as well.CONCLUSION:The BOL has certain therapeutic effect on theexperiment hepatoflbrosis.Its mechanisms might include:protecting the function of liver cells,inhibiting excessivesynthesis and ascretion of extracelluar matrix from hepaticatellete cells,relieving the capillarization of hepaticsinusold,Improving liver micro-clrculation,and regulatingimmune function.     

2002—2012年大邑县艾滋病监测

目的了解大邑县的艾滋病流行特征,为科学制定防治策略提供依据。方法通过《艾滋病综合防治信息系统》收信、整理、分析资料。结果2002—2012年大邑县共发现HIV／AIDS145例,死亡35例,年龄集中在25～45岁。男性100例,女性45例,男女性别比为2．22：1。职业以农民为主（62．76％）,文化程度以初中为主（50．34％）,婚姻状况以已婚有配偶为主（50．34％）,感染途径以异性传播为主（88．97％）。结论大邑县艾滋病病例以男性青壮年农民为主,应加强高危人群,特别是农民的艾滋病防控工作。     

实验性肝细胞癌铁铜含量与MR成象的关系

目的初步阐明在MRI常规SE序列,铁、铜元素对肝细胞癌TIWI信号的影响.方法20g/LDEN灌喂Wistar大鼠(n=80),诱癌成功后行常规SE序列T1WI,T2WI扫描,对照组(n=20)不灌喂DEN.扫描后处死动物取肝组织与肝癌组织作HE、普鲁士蓝染色,并测定组织内水、铁、铜含量.结果肝癌与癌周组织含水量无显著差异,肝癌内铁含量低于癌周肝组织,肝癌内的铜含量高于癌周肝组织,在呈低信号的肝癌组织内含铁血黄素明显高于其他信号强度类型,在呈高信号的肝癌组织内明显高于其他信号强度类型的肝癌组织.结论水的含量不是影响肝癌MR表现的主要因素,肝癌内含铁血黄素在SE T1WI呈低信号起作用,肝癌内铜的蓄积对SET1WI呈高信号起作用.     

Exploration of the immunoreactivity of the Traditional Chinese medicine Shenrouyangzhentang to vasoactive intestinal polypeptide

AIM: To study the immunoreactivity of the Chinese medicine Shenrouyangzhentang to vasoactive intestinal polypeptide (VIP) and its therapeutic mechanism.METHODS: The immunoreactivity of the Chinese medicine Shenrouyangzhentang to VIP was detected in the plasma of 20 normal people and 20 patients with Piyinxu (Spleen Yin deficiency) using the radioimmunoassay (RIA) method.RESULTS: The maximum binding rate B₀/T was 53.29%, the non-specific binding rate N₀/T was 1.170%, and the VIP standard curve was Y = 0.81983 + 0.44319X - 0.28927X², R² = 0.990. The VIP content in Shenrouyangzhentang was 106.6 ng/L ± 20 ng/L), while it was 90.16 ng/L ± 15 ng/L in normal human plasma and 63.25 ng/L ± 11 ng/L in the plasma of Pixinxu patients. The difference between normal plasma and Pixinxu patient plasma was statistically significant (P < 0.05).CONCLUSION: The Chinese medicine Shenrouyangzhentang demonstrated VIP immunoreactivity similar to that of normal plasma. The (vasoactive intestinal polypeptide) VIP content in Pixinxu patient plasma was lower than that in healthy subjects (P < 0.05).     

急性心肌梗死生化指标的研究现状

近年,急性心肌梗死(ＡＭＩ)生化指标检测出现了许多新的变化,主要体现三个方面:(1)测定心肌酶谱转向特异蛋白测定;(2)单一诊断转向尽早诊断与预后判断;(3)实验室分析转向实时测定。　　生化指标检测对急性心肌梗死的诊断1.心肌酶学:肌酸激酶(ＣＫ)、天门冬氨酸转氨酶、乳酸脱氢酶及其同工酶等指标由于对心肌损伤的特异性差,组织分布广,被具有高度特异性的ＣＫ同工酶(ＣＫ-ＭＢ)所取代。于90年代初,ＣＫＭＢ成为诊断ＡＭＩ的金标准。偶有保留ＣＫ,不过仅偶有保留作观察对照,且取高临界值(≥2倍)。实际上,该取值不完全适合ＡＭＩ的个体诊断[1]…     

Printable and Machinable Dental Restorative Composites for CAD/CAM Application—Comparison of Mechanical Properties,Fractographic, Texture and Fractal Dimension Analysis

Thanks to the continuous development of light-curing resin composites it is now possible to print permanent single-tooth restorations. The purpose of this study was to compare resin composites for milling -Gandio Blocks (GR), Brilliant Crios (CR) and Enamic (EN) with resin composite for 3D printing—Varseo Smile Crown plus (VSC). Three-point bending was used to measure flexural strength (σ_f) and flexural modulus (E_f). The microhardness was measured using a Vickers method, while fractographic, microstructural, texture and fractal dimension (FD) analyses were performed using SEM, optical microscope and picture analysis methods. The values of σ_f ranged from 118.96 (±2.81) MPa for EN to 186.02 (±10.49) MPa for GR, and the values of E_f ranged from 4.37 (±0.8) GPa for VSC to 28.55 (±0.34) GPa for EN. HV01 ranged from 25.8 (±0.7) for VSC to 273.42 (±27.11) for EN. The filler content ranged from 19–24 vol. % for VSC to 70–80 vol. % for GR and EN. The observed fractures are typical for brittle materials. The correlation between FD of materials microstructure and E_f was observed. σ_f of the printed resin depends on layers orientation and is significantly lower than σ_f of GR and CR. E_f of the printed material is significantly lower than E_f of blocks for milling.