雷公藤多苷治疗特发性膜性肾病的Meta分析

目的:评价雷公藤多苷(multi-glycoside of tripterygium wilfordii,GTW)治疗特发性膜性肾病(idiopathic membranous nephrology,IMN)的临床疗效和不良反应。方法:检索cochrane图书馆、Pub Med、EMbase、CNKI、万方、维普等数据库,收集关于雷公藤多苷治疗特发性膜性肾病的RCT研究论文。结果:共纳入10篇RCT和CCT文献,共577例患者纳入此次研究。Meta分析显示雷公藤多苷治疗组(GTW组)与对照组相比,可提高IMN的完全缓解率(RR=1. 60,95%CI=1. 31～1. 95,P 0. 01),而总缓解率差异有统计学意义(RR=1. 42,95%CI=1. 66～1. 74,P 0. 01)。剔除3篇随访时间小于36周的研究,完全缓解率较前无明显变化(RR=1. 45,95%CI=1. 15～1. 82,P 0. 01)。GTW组复发率、不良反应发生率与对照组比较均无统计学意义,GTW组的不良反应主要是肝功能恶化、消化道症状、月经紊乱。结论:就治疗IMN而言,相较于其他类药物,雷公藤多苷的完全缓解率要更高,并且达到完全缓解率的时间较短。     

雷公藤多苷对抗Thy1.1抗体肾炎肾小球内炎症细胞浸润的抑制作用

目的:观察雷公藤多苷(multi-glycoside of Tripterygium wilfordii.,GTW)对肾小球内浸润的炎症细胞及其相关细胞因子的影响,进一步阐明GTW在体内的抗炎作用.方法:经尾静脉注射500μg单克隆抗体(monoclonal antibody,mAb)1-22-3建立2种大鼠抗Thy1.1抗体肾炎模型.将模型鼠随机分为GTW干预组(GTW组)和蒸馏水干预组(对照组).实验1中,在注射抗体前3 d,经灌胃给予较大剂量GTW(75.mg·kg~(-1)·d~(-1))或蒸馏水,直至造模后第7天,处死大鼠;实验2中,在第2次注射抗体同时,经灌胃给予中等剂量GTW(50 mg·kg~(-1)·d~(-1))或蒸馏水,直至造模后第42天,处死大鼠.分别观察7 d内或42 d内模型鼠24 h尿蛋白排泄量(urinary protein excretion,Upro)动态变化;在处死大鼠后,取出肾脏,分别观察肾小球内浸润的巨噬细胞(macrophage,MΦ),T淋巴细胞变化和肾组织内白介素(interleukin,IL)-2和干扰素(interferon,IFN)-γ核酸的表达.结果:实验1中,可逆性抗Thy1.1抗体肾炎模型鼠肾小球内浸润的MΦ明显增多,较大剂量的GTW可以减少模型鼠肾小球内MΦ的浸润和肾组织内IL-2核酸表达;实验2中,不可逆性抗Thy1.1抗体肾炎模型鼠肾小球内浸润的活性MΦ,T淋巴细胞明显增多,中等剂量的GTW可以改善模型鼠肾小球内活性MΦ,T淋巴细胞的浸润,以及肾组织内IL-2,IFN-γ核酸表达;对于2种模型,GTW都有减少蛋白尿的作用.结论:不同剂量的GTW在体内对抗Thy1.1抗体肾炎.肾小球内炎症细胞浸润有抑制作用,这些作用与改善肾组织内IL-2和IFN-γ核酸表达有关.     

临床高剂量雷公藤多苷对幼年大鼠生育能力的影响

目的探讨儿科临床高剂量(1.5mg/㎏)雷公藤多苷(GTW)对幼年大鼠生育能力的影响。方法雄性、雌性SD幼鼠各50只,均随机分为空白组与GTW组,每组25只,GTW按每日9mg/kg给药,用药12周后,雄鼠按1:1与健康成年雌性大鼠合笼,雌鼠按2:1与健康成年雄鼠合笼,分3次合笼,每次合笼时间为2周,合笼后观察雌鼠受孕率、仔鼠离乳存活率。结果雌、雄鼠每次合笼后GTW组受孕率、离乳存活率与空白组比较差异均无统计学意义。结论临床高剂量GTW对幼年大鼠发育为成年大鼠后生育能力和所生仔鼠的生长发育未见明显影响。     

雷公藤多苷对糖尿病肾病模型肾小球损伤的影响

目的:观察雷公藤多苷(multi-glycoside of Tripterygium wilfordii,GTW)对糖尿病肾病模型肾小球损伤的改善效果.方法:运用链脲佐菌素(streptozotocin,STZ)建立糖尿病肾病模型,以GTW干预,并设立正常、安慰剂以及贝那普利对照组.比较体重、尿微量白蛋白(urine albumin,UAlb)、血糖(blood glucose,BG)、血清肌酐(serum creatinine,Scr)、血清尿素氮(blood urea nitrogen,BUN)以及肾小球形态(细胞总数、细胞外基质)的变化,并检测肾小球中α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA),Ⅰ型胶原的表达水平.结果:GTW和贝那普利都可以减少模型鼠UAlb;对于模型鼠肾小球硬化性损伤,如系膜细胞增生,α-SMA和Ⅰ型胶原异常表达等,GTW的作用强于贝那普利,肾小球总细胞数GTW组(54.44±2.41),贝那普利组(67.83±4.41),P＜0.05;α-平滑肌肌动蛋白积分GTW组(1.98±0.52),贝那普利组(2.27±0.46),P＜0.05;Ⅰ型胶原积分GTW组(2.11±0.37),贝那普利组(2.88±0.58),P＜0.05.结论:GTW改善DN肾小球损伤的效果包括减少蛋白尿和改善肾小球硬化.     

雷公藤治疗肾小球肾炎的研究近况

雷公藤(Tripterygium Wilfordii Hook．f．)系卫矛科雷公藤属木质藤本植物。早在1936年赵承嘏首先从雷公藤根部提取到萜类色素——雷公藤红(tripter- ine),至今已提取到近70种成分,主要为生物碱类、二萜、三萜类、倍半萜类及糖类。现代研究表明,雷公藤具有抗炎、调节免疫、抗肿瘤、抑制生殖等多种药理活性。目前,雷公藤广泛应用于各种原发性和继发性肾小球肾炎的治疗,取得了满意的疗效,现将近年来雷公藤治疗肾小球肾炎的实验与临床研究综述如下。     

五子四物瓜石汤对抗雷公藤多苷所致消化系统毒性的研究

目的 :研究五子四物瓜石汤 (WT)对雷公藤多苷 (GTW )所致大鼠及小鼠消化系统毒性的对抗作用。方法 :分别以大、中、小三个剂量WT与等剂量GTW合用 ,以单用GTW组为对照 ,观察各组大鼠体重、胃液成份、消化系统脏器病理及小鼠小肠推进的改变。结果 :中剂量WT可显著对抗GTW所致大鼠食欲减退、腹泻及体重下降 ,并能显著解除GTW对小鼠小肠推进性蠕动的抑制 ;GTW及WT对大鼠胃液成份及消化系统脏器病理无明显影响。结论 :WT可显著对抗GTW所致大鼠及小鼠消化系统的毒性。     

雷公藤的免疫抑制作用及在移植免疫中的应用

雷公藤(Tripterygium wilfordii Hook f.)又名莽草、黄藤根、莱虫药、断肠草等,为我国首创的药物,应用于临床已有悠久的历史。雷公藤泛指卫矛科(Celastraceae)雷公藤属(Tripterygium Hook f.)植物的根及根茎。自60年代末发现雷公藤治疗类风湿性关节炎有效后;此药开始受到重视,其药理作用及有效成分的提取等项研究也日益广泛。目前已从雷公藤中提取出的多种化学成分中主要有效的是雷公藤生物碱、二萜、三萜及甙类化合物。临床证明雷公藤水煎剂及多种提取物治疗类风湿性关节炎、系统性红斑狼疮、白塞氏病、肾炎及一些自身免疫性疾病     

益肾饮拮抗雷公藤多苷对系膜增生性肾炎雌性大鼠性腺损害的研究

目的:探讨益肾饮拮抗雷公藤多苷对系膜增生性肾小球肾炎雌性大鼠性腺损害的作用机制。方法:实验大鼠随机分为正常对照组、模型组、雷公藤多苷组、益肾饮组、雷公藤多苷和益肾饮合用组。制备大鼠系膜增生性肾小球肾炎模型;以雷公藤多苷和益肾饮灌胃;观察各组大鼠24 h尿蛋白定量,性激素孕酮、雌二醇,卵巢、子宫的病理变化。结果:益肾饮和雷公藤多苷各自治疗和合用治疗大鼠系膜增生性肾小球肾炎后,24 h尿蛋白定量减少;雷公藤多苷组卵巢脏器指数降低,病理改变明显;益肾饮与雷公藤多苷合用组性腺损害不明显。结论:益肾饮联合雷公藤多苷治疗系膜增生性肾小球肾炎,能有效地减少尿蛋白量,益肾饮可拮抗雷公藤多苷对雌性大鼠系膜增生性肾小球肾炎的性腺损害。     

益肾饮拮抗雷公藤多苷对雄性肾炎大鼠生殖系统毒性的研究

目的:探讨益肾饮拮抗雷公藤多苷对雄性大鼠系膜增生性肾小球肾炎性腺损害的作用机制.方法:大鼠随机分为正常组、模型组、雷公藤多苷组、益肾饮组、雷公藤多苷和益肾饮合用组.参照文献制备大鼠系膜增生性肾小球肾炎模型,以雷公藤多苷和益肾饮灌胃;观察各组大鼠24小时尿蛋白定量、性激素睾酮及睾丸病理学变化.结果:益肾饮和雷公藤多苷及其合用治疗大鼠系膜增生性肾小球肾炎后,24小时尿蛋白定量减少;雷公藤多苷组睾丸脏器指数降低,病理改变明显;益肾饮与雷公藤多苷合用组性腺损害不明显.结论:益肾饮能够明显减轻雷公藤多苷对系膜增生性肾小球肾炎雄性大鼠的性腺损害.     

菟丝子黄精颗粒剂对雷公藤多苷所致生殖损伤雌鼠卵巢损伤及smad4 mRNA表达的影响

目的 研究雷公藤多苷(GTW)对卵巢组织smad4 mRNA表达的影响以及菟丝子与黄精的干预作用.方法 采用4周龄SD雌鼠,将实验大鼠据体质量随机分3组,分别为空白对照组、GTW组、干预组,连续灌胃12周后处死动物检测指标.结果 GTW组卵巢次级卵泡数值最小,与空白组间差异有统计学意义(P＜0.05),与干预组间差异也有统计学意义(P＜0.01),空白组与干预组间差异没有统计学意义(P＞0.05);黄体数比较各组间差异没有统计学意义(P＞0.05).GTW组smad4 mRNA表达与空白组间差异有统计学意义(P＜0.01),与干预组间差异也有统计学意义(P＜0.05);空白组与干预组间差异没有统计学意义(P＞0.05).结论 雌性幼鼠服用雷公藤多苷可造成卵巢组织病理改变,其中smad4 mRNA表达的下降可能是GTW导致雌性生殖损伤的关键点之一.中药菟丝子、黄精可以促进该过程的修复.     

Toxicogenomic analysis of the gene expression changes in rat liver after a 28-day oral Tripterygium wilfordii multiglycoside exposure

Ethnopharmacological relevance

Tripterygium wilfordii multiglycoside (GTW), which is an extract derived from Tripterygium wilfordii Hook.f., has been used for the treatment of rheumatoid arthritis and other immune diseases in China. However, its potential hepatotoxicity has not been completely investigated.

The aim of the study

The aim of the study was to determine the hepatotoxicity of GTW in Wistar rats and to investigate the underlying cellular mechanism further by microarray analysis.

Materials and methods

Doses of GTW at 60, 100 and 120 mg/kg/day were administered by oral gavage for subchronic toxicity in Wistar rats. Changes in the hepatic gene expression were identified with oligonucleotide microarrays at the 100-mg/kg/day dose level to study the hepatotoxic mechanism of GTW.

Results and conclusions

A number of changes in the body weight and food consumption, absolute and relative liver weight, biochemical analysis and histopathology were observed after the subacute exposure to GTW, and a dose-dependent hepatotoxicity was observed. A total of 1312 genes were found to be significantly altered (2-fold, P < 0.05), including 582 up-regulated genes and 730 down-regulated genes. According to our biological pathway analysis, the GTW resulted in aberrant gene expression in metabolic pathways and the peroxisome proliferator-activated receptor (PPAR) signaling pathway and cellular stress. Real-time PCR analyses of several genes verified these results. Consequently, our gene expression microarray study will be useful for future GTW hepatotoxicity studies.     

雷公藤多苷对不可逆性肾小球硬化模型系膜损伤的抑制作用

目的观察雷公藤多苷(GTW)对不可逆性肾小球硬化模型系膜损伤的抑制作用,阐明其在体内延缓肾小球硬化的分子药理机制。方法运用2次注射单克隆抗体(mAb)1223的方法,建立大鼠不可逆性肾小球硬化模型,以经口灌胃GTW(50mg·kg^-1BW)干预42d,并设立对照组。比较24h尿蛋白排泄量(Upro)、肾功能(Scr,BUN)以及肾小球形态学(LM,IF)变化,并检测(RTPCR)模型鼠肾组织中I型胶原(collagentypeI)、转化生长因子(TGFβ)mRNA表达水平。结果GTW抑制肾小球硬化模型鼠Upro和系膜增殖。肾小球总细胞数GTW组(65.67±3.43),对照组(87.02±2.41),P<0.05;细胞外基质积分GTW组(1.20±0.06),对照组(2.77±0.23),P<0.05;α平滑肌肌动蛋白积分GTW组(1.75±0.33),对照组(2.62±0.15),P<0.05;I型胶原积分GTW组(1.68±0.31),对照组(2.06±0.24),P<0.05。GTW使肾小球硬化模型鼠肾组织中collagentypeI,TGFβmRNA表达水平下调53.5%和14.7%,与对照组比较,二者差异均有显著性(P<0.05)。结论GTW抑制肾小球硬化模型鼠Upro和系膜增殖的作用是通过减少细胞因子mRNA的表达来实现的。     

Multi-glycoside of Tripterygium wilfordii Hook. f. reduces proteinuria through improving podocyte slit diaphragm dysfunction in anti-Thy1.1 glomerulonephritis

Ethnopharmacological relevance

Multi-glycoside of Tripterygium wilfordii Hook. f. (GTW) has been proved clinically effective in reducing proteinuria in chronic kidney disease in China. However, the mechanisms involved are still unclear. In this study we examined the effects of GTW at the different dosages on proteinuria and podocyte slit diaphragm (SD) dysfunction in anti-Thy1.1 glomerulonephritis (GN).

Materials and methods

Rats with anti-Thy1.1 GN were divided into 2 groups, a GTW group and a vehicle group, and sacrificed at 30 min, on day 7, and on day 14 in Experiments 1, 2 and 3, respectively. The administration of GTW at the moderate and high doses was started 3 days before or at the same time of antibody injection till sacrifice. Proteinuria was determined in Experiments 1, 2, and 3. After sacrifice, the staining intensity of SD-associated key functional molecules including nephrin and podocin, podocyte structure, mesangial change, macrophage infiltration, and blood biochemical parameters were examined, respectively. Protein and mRNA expressions of nephrin and podocin in glomeruli were also investigated. Besides, liver histological characteristics were analyzed.

Results

In Experiment 1, GTW pretreatment at the medium dose (75 mg/kg body weight) caused no influence on the induction of anti-Thy1.1 GN and the basal nephrin expression. In Experiment 2, the high dosage (100 mg/kg body weight) of GTW ameliorated proteinuria, the distribution of nephrin and podocin, mesangial proliferation, and the activated macrophage accumulation, as compared with vehicle group (P < 0.05). Additionally, it increased mRNA and protein expressions of nephrin and podocin in glomeruli on day 7, but had no influence on podocyte structure. In Experiment 3, the medium dosage (75 mg/kg body weight) of GTW improved proteinuria, the partial matrix expansion, and the distribution of nephrin and podocin on day 14, as compared with anti-Thy1.1 GN rats (P < 0.05). GTW at the high or moderate dose did not affect hepatic function on day 7 and on day 14.

Conclusions

Podocyte SD dysfunction, such as the disordered distribution and down-regulation of nephrin and podocin expression, is critically involved in the pathogenesis of anti-Thy1.1 GN induced by mAb 1-22-3. The restoration of the distribution and expression of nephrin and podocin by GTW could be an important mechanism by which GTW ameliorates proteinuria and podocyte SD dysfunction.     

Comparison of toxic reaction of Tripterygium wilfordii multiglycoside in normal and adjuvant arthritic rats

Aim of the study

Tripterygium wilfordii multiglycoside (GTW), an authorized Chinese patent drug, is used for treatment of rheumatoid arthritis and other immune disease. This study was to determine whether GTW induced different toxic reactions in adjuvant arthritis rats (AA rats) compared to those in normal rats.

Materials and methods

To prepare arthritic rat model, male Sprague-Dawley (SD) rats were immunized by injecting complete Freund's Adjuvant into right hind footpad. And then, GTW was given to rats intragastrically at dosage of 7 or 105 mg kg⁻¹ day⁻¹ from day 15 to day 28 after immunization. Routine clinical parameters and histopathologic changes of liver, kidney and testis were examined. Metabolic profiling in serum of groups was analyzed by LC-MS. A principal component analysis (PCA) and partial-least-squares discriminate analysis (PLS-DA) were carried out combined with mass spectrometry (MS) data set. All the quantitative data were performed by two-way ANOVA analysis following Student's t-test.

Results and conclusions

Treatment with GTW at both doses could diminish the right and left hind paws swelling. There was slight lipoid degeneration in hepatic tissue of normal rats treated by high dose of GTW, but there were not distinctly pathological changes in hepatic tissue of AA rats treated by GTW. Compared normal rats administered with GTW, no statistically significant difference in the serum alanine aminotransferase (ALT), creatinine (CRE), and blood urea nitrogen (BUN) levels were observed. However, the serum aspartate aminotransferase (AST) level was significant decreased in AA rats under exposure GTW compared with normal rats in the same conditions (p < 0.05), which indicated that GTW could offer a different liver toxic reaction in normal and AA rats. The metabolic analysis showed that a clear separation of PCA and PLS-DA score spot in normal rats, but not separation was seen in AA rats perturbed with low dosage GTW. The result indicated low dosage GTW might arouse a general toxic in normal rats but not in AA rats. The biomarker analysis showed that the level of lysophosphatidylcholines (LPCs) was down-regulated, but the level of ursodeoxycholic acid (UDCA) and chenodexycholic acid (CDCA) was up-regulated in AA rats compared with normal rats under exposure GTW. According to pathway analysis of metabolic markers, we conceived that LPC, UDCA and CDCA were the critical intermediates of choline and fatty acid metabolism. And the lipid metabolism was a correlative outcome of GTW induced toxicity in the liver in physiological condition animals. Taken together, GTW could induce different toxic reactions between normal and AA rats, and the lipid metabolism might be part of the mechanism for the hepatic lipidosis or the other liver injury.     

“五子四物瓜石汤”对雷公藤多苷所致雄性大鼠生殖系统毒性的对抗作用及其机制研究

目的 研究自拟中药复方“五子四物瓜石汤”（WT）对雷公藤多苷（GTW）所致雄性大鼠生殖系统毒性的对抗作用及其机制。方法 雄性大鼠分别ig大，中，小剂量的WT与固定剂量的GTW，连续给药80d，以单独igGTW或NS为对照，观察各组雄性大鼠睾丸脏器指数，精子精，精子活率，睾丸病理结构的变化。并用免疫放射分析法（IRMA）测定各组雄性大鼠血清性激素水平。结果 与单用GTW组比较，大或中剂量WT可使睾丸重量明显增加，精子精明显升高，精子活率显著提高，其中大剂量WT可完全对抗GTW对生殖上皮的损害，使睾丸结构完全正常，血清睾酮维持了正常水平。结论 “五子四物瓜石汤”能显著对抗雷公藤多苷对雄性大鼠生殖系统的毒性作用，其对抗作用与WT保护睾丸生精上皮免受GTW的损害，并使血清睾酮水平维持于正常有关。     

野山楂根拮抗雷公藤多苷对雄性大鼠生殖损伤作用的研究

目的:观察中药野山楂根水煎剂对雷公藤多苷所致雄性不育大鼠生育力的影响。方法:将成年雄性大鼠随机分组,分别给予雷公藤多苷片溶液(10 mg.kg^-1),野山楂根水煎剂(1.8,5.4,18 g.kg^-1)+雷公藤多苷片溶液(10 mg.kg^-1)和蒸馏水(1 mL.kg^-1)灌胃,8周后停用雷公藤多苷,中药组继续行野山楂根灌胃。4周后各组动物与成年雌性大鼠交配,观察交配雌鼠妊娠率和平均胚胎、黄体数。测定雄鼠生殖器官性腺脏器指数、附睾精子计数、活力、存活率,观察各组动物睾丸、附睾组织病理和超微结构改变,放射免疫法测定血清和睾丸组织睾酮(T),血清卵泡刺激素(FSH)和促黄体生成素(LH)含量。结果:与雷公藤多苷模型组比较,野山楂根组交配雌鼠妊娠胎数、雄鼠睾丸、附睾脏器指数以及附睾精子计数、活力均有提高(P<0.05);野山楂根组精子超微结构较模型组有明显恢复;睾丸组织匀浆T显著高于模型组(P<0.01)。结论:野山楂根能够拮抗雷公藤多苷对雄性大鼠的生殖损伤作用,提高不育症模型雄鼠的生育力。     

雷公藤多苷对小鼠生殖功能的影响及肉苁蓉的干预作用

目的 观察雷公藤多苷（GTW）对小鼠生殖功能的影响及肉苁蓉预防生殖毒性的作用。方法将雄性小鼠随机分为空白对照组、GTW组、肉苁蓉组、停药组。空白组灌胃1％羧甲基纤维素（CMC）溶液;GTW组灌胃GTW混悬液;肉苁蓉组灌胃GTW混悬液共20d,第28天起同时灌胃肉苁蓉水煎剂加GTW混悬液;停药组灌胃GTW混悬液共20d,第28天起改为灌胃1％CMC溶液。各组灌胃20d后与雌鼠以1：2比例合笼;第28天起分笼继续给药,于48d后再次合笼。观察两次合笼雌鼠受孕率及雄鼠睾酮水平的变化。结果随着雷公藤多苷给药时间的延长,雌鼠受孕率呈下降趋势,雄鼠睾酮水平亦呈减低的变化趋势。肉苁蓉干预后,受孕率由15％上升到35％;停药组受孕率由10％上升到30％,与GTW组比较有显著性差异（P〈0．01）,与空白组比较无统计学差异（P〉0．05）。结论GTW对雄性小鼠的生殖毒性呈时间依赖性,停用后可自行恢复;肉苁蓉可以改善GTW对雄鼠生殖系统的抑制作用。     

抗Thy1.1抗体诱导的肾炎模型及其在中药研究中的应用

抗Thy1.1单克隆抗体诱导的抗Thy1.1抗体肾炎模型广泛应用于人类系膜增殖性肾炎的研究领域。该模型的病理特征包括补体依赖性系膜溶解、肾小球内炎症细胞浸润、显著的蛋白尿,以及急性或进展性系膜损伤。文章综述了经静脉注射单克隆抗体1-22-3诱导的可逆性抗Thy1.1抗体肾炎模型和不可逆性抗Thy1.1抗体肾炎模型的病理特征；阐述了肾小球内炎症细胞浸润和各种损伤性细胞因子的变化规律；分析了包括系膜细胞增殖和细胞外基质沉积在内的复杂的系膜损伤病变过程。运用该模型,可以说明雷公藤多苷、柴苓汤等中药对系膜损伤和蛋白尿的药理作用,并且,在分子水平阐明这些药物对各种损伤因子的作用机制。     

红景天苷对神经系统疾病药理作用的研究进展

红景天苷是植物红景天的提取物,也是其主要有效成分.近年研究发现红景天苷具有神经保护、清除自由基、调节中枢神经递质、促进神经修复及抗神经细胞凋亡等多种作用,有望应用于多种神经变性疾病及脑缺血性疾病.该文综述红景天苷对神经系统疾病的药理作用研究进展,为进一步研究、开发和利用红景天提供参考.     

雷公藤多甙对实验性系膜增殖性肾炎蛋白尿及系膜损伤的影响

目的 观察雷公藤多甙（multi-glycoside of tripterygium wilfordii Hook．f．,GTW）在体内对实验性系膜增殖性肾炎蛋白尿及系膜损伤的影响。方法 运用单克隆抗体（monoclonal antibody,mAb）1-22-3建立大鼠可逆性抗Thy1．1抗体肾炎（简称“抗Thy1．1肾炎”）模型,以GTW干预,并设立对照组。比较24h尿蛋白排泄量（urinary protein excretion,Upro）、肾功能（serum creatinine,SCr;blood urea nitrogen,BUN）、血浆总蛋白（total plasma protein,TP）以及肾小球形态学变化,并检测肾组织中增殖因子（platelet-derived growth factor-BB,PDGF-BB;transforming growth factor-β,TGF-β）mRNA表达水平。结果 GTW抑制抗Thy1-1肾炎模型Upro和系膜损伤;抗Thy1．1肾炎模型肾组织中增殖因子（PDGF-BB、TGF-β）mRNA表达水平与正常组比较,分别为其2．84倍与1．64倍,GTW使PDGF-BB mRNA过高表达水平下调33．1％,与对照组比较,差异有显著性（P〈0．05）。结论 GTW可减少系膜增殖性肾炎模型蛋白尿,抑制系膜细胞增殖和细胞外基质沉积;这些作用可能与下调肾组织增殖因子（PDGF-BB）mRNA的表达有关。