不同甲状腺机能状态对地西泮氧化代谢的影响

本文建立了大鼠的甲状腺机能低下（甲低）和甲状腺机能亢进（甲亢）的动物模型,用HPLC方法测定地西泮的代谢物,以研究甲低和甲亢对SD大鼠单次口服地西泮氧化代谢的影响,甲低组大鼠地西泮的N－去甲基化代谢物去甲地西泮的血药浓度（AUC）升高,消除减慢,说明甲低对地西泮的N－去甲基化影响不大,而主要影响去甲地西泮的C3－羟化代谢,不同程度甲亢对地西泮氧化代谢的影响有所不同,轻度和中度甲亢组去甲地西泮血药浓度（AUC）低于对照组,消除加快;而重度甲亢组去甲地西泮血药浓度（AUC）略高于对照组,消除减慢。轻、中、重度甲亢大鼠地西泮C3－羟化代谢物替马西泮血药浓度（AUC）都不同程度地低于对照组,表明仅在重度甲亢对去甲地西泮的C3－羟化代谢才有抑制性影响,但不同程度甲亢均抑制地西泮的C3－羟化代谢。     

pH恒定条件下兔甲状腺腺泡电位的测定

本文报告了在采用“PH自动控制系统”控制培养液pH值的条件下,用微电极测定兔甲状腺切片腺泡细胞膜电位与泡腔电位的氏液p0.02,氧饱和)表面灌流下,0～3h内细胞膜电位相对稳定,平均为—33.31.6mv(n=19);～4h膜电位趋于去极化,～5h膜电位较～1h明显去极化、p<0.05),但至25～27h膜电位仍可维特在—22.7±2.0mv(n=7),腺泡腔电位始终没有明显变化,0～27h内平均为—12.4±0.4mv,(n=38)。与其他作者结果比较,我们认为在平衡盐溶液中甲状腺细胞膜电位可能为—42mv左右,腺泡腔电位为—12mv左右;本实验条件优于国外采用5％CO_2混合气控制pH的条件。     

不同甲状腺机能状态对地西泮药代动力学的影响

用大鼠建立甲状腺机能低下(甲低)和甲状腺机能亢进(甲亢)的动物模型。以HPLC法测定地西泮血药浓度,研究甲低和甲亢对地西泮药代动力学的影响。结果表明,甲低组大鼠地西泮血药浓度显著高于对照组(P<0.05),Cmax升高,AUC增大,吸收T_1/2延长,Vd减小,消除减慢。轻度甲亢组地西泮血药浓度、Cmax及AUC与对照组相比无差异,但随着甲亢程度加重,上述指标逐渐增高。轻、中度甲亢组地西泮吸收基本不变,Vd减小,消除加快;重度甲亢组吸收明显加快,Vd减小,消除减慢。提示不同甲状腺机能状态对地西泮药代动力学的影响不同。     

不同甲状腺机能状态对地西泮氧化代谢的影响(英)

本文建立了大鼠的甲状腺机能低下（甲低）和甲状腺机能亢进（甲亢）的动物模型。用HPLC方法测定地西泮的代谢物，以研究甲低和甲亢对SD大鼠单次口服地西泮氧化代谢的影响。甲低组大鼠地西泮的N－去甲基化代谢物去甲地西泮的血药浓度（AUC）升高，消除减慢。说明甲低对地西泮的N－去甲基化影响不大，而主要影响去甲地西泮的C_3－羟化代谢。不同程度甲亢对地西泮氧化代谢的影响有所不同。轻度和中度甲亢组去甲地西泮血药浓度（AUC）低于对照组，消除加快；而重度甲亢组去甲地西泮血药浓度（AUC）略高于对照组，消除减慢。轻、中、重度甲亢大鼠地西泮C_3－羟化代谢物替马西泮血药浓度（AUC）都不同程度地低于对照组，表明仅在重度甲亢对去甲地西泮的C_3－羟化代谢才有抑制性影响，但不同程度甲亢均抑制地西泮的C_3－羟化代谢。     

甲状腺机能对肝脏药物代谢酶的影响

甲状腺机能对肝脏药物代谢酶的影响但汉雄（湖北咸宁医学院药理教研室，咸宁４３７１００）中国图书分类号Ｒ９６３；Ｒ５８６甲状腺是一个重要的内分泌器官。临床上甲状腺机能状态的改变是常见现象，其对药代动力学的影响多有报道〔１〕。本文旨在利用实验性甲亢和甲低模...     

甲状腺机能亢进症误诊分析

甲状腺机能亢进症误诊分析大同市煤炭工业医院（０３７００４）蒋树之，黄振芬大同市第五人民医院李自荣为了提高甲亢的诊断水平，现将我院门诊和从其它医院转来做甲功检查的误诊病例分析如下。资料及分析１．误诊为胃肠道疾患。例１：男性，６０岁。主因消瘦、腹泻（每日...     

甲状腺机能亢进漏诊10例报告

甲状腺机能亢进症,是甲状腺功能增高,分泌激素增多所致的一组常见的内分泌疾病。全身各脏器由甲状腺激素过多引起一系列病理生理变化,临床上呈高代谢症群、神经心血管系等兴奋亢进,甲状腺肿大等特征。在临床上由于症状不够典型、认识不足而误诊。现将我院收治甲亢误诊10例报告如下。     

青少年甲状腺机能亢进症的手术治疗

目的：探讨青少年甲状腺机能亢进症的手术治疗效果。方法：用卢戈氏液或心得安加卢戈氏液作为术前准备,对23例患者行双侧甲状腺次全切除术。结果：本组无手术死亡,无神经损伤,不需输血,术后恢复快。随访6个月～5年,甲亢治愈率100％,无复发及甲状腺功能低下。结论：手术治疗青少年甲亢疗效迅速确切,不影响其生长发育。     

甲状腺机能亢进症误诊1例

患者，女，47岁，平素健康，于1993年8月感活动后心悸、胸闷、气短，在当地医院做心电ST－T改变，诊断为冠心病，经用扩冠药及能量合剂治疗，症状有所缓解，此后间断服用扩冠药物治疗。于1994年先后两次去外院检查，均诊断为冠心病。此期间，患者出现多汗、失眠症状，按医嘱加用谷维素、更年康等药物治疗．于1994年10月，因上述症状加重人我院．查体：KT36．8C，P102次份，BP20．0／9．3kPa，自动体位，无突眼，甲状腺无肿大，，心界不大，心率98－112次／分，闻及早搏3－4次／分，于心尖部闻及Ⅱ级收缩期杂音，肝不大。化验：血尿常规…     

甲状腺侧叶切除术治疗孤立甲状腺结节的可行性研究

目的分析甲状腺侧叶全切术治疗孤立甲状腺结节的合理性与安全性。方法回顾性分析169例孤立甲状腺结节患者甲状腺侧叶全切术的治疗效果。结果141例为甲状腺良性病变,28例为甲状腺癌。术后均未出现甲状腺功能低下,169例患者均存活,无癌复发。结论甲状腺侧叶全切术是治疗孤立甲状腺结节的安全和有效的术式。     

Anti-atherosclerotic effect of geniposidic acid in a rabbit model and related cellular mechanisms

Abstract

Context: Geniposidic acid, one of the main active ingredients in Gardenia jasminoides J. Ellis (Rubiaceae), may also possess important pharmacological activities for cardiovascular disorders similar to other derivatives, such as geniposide.

Objective: To evaluate its anti-atherosclerosis (anti-AS) effect, the related pharmacological activities and possible cellular mechanisms were studied.

Materials and methods: Thirty rabbits were randomly divided into normal control group, model control group, and geniposidic acid subgroups. In the AS model, its effects on the intima/media thickness ratio and aortic morphology were observed. In the study of primary cultured endothelial cells (ECs) and human umbilical artery smooth muscle cells (HUASMCs), its activities on both ECs and HUASMCs proliferation, HUASMCs' migration were also studied.

Results: Compared with the model control group, the plaque area, intima/media thickness ratio, and intimal foam cells number in geniposidic acid (80, 160, and 240?mg/kg) subgroups were significantly improved (p?<?0.05). By HE staining, the activities of geniposidic acid on relieving ECs shedding and improving aortic morphology disorders were also demonstrated. From the results of CCK-8 testing, only 100?μg/ml geniposidic acid performed significant inhibition on SMC proliferation. The relative IC₅₀ of geniposidic acid on SMC inhibition was 87.73?μg/ml. Geniposide acid also showed promotion effect on ECs proliferation, and the related ED₅₀ of geniposidic acid was 86.05?μg/ml. Besides, only 50 and 100?μg/ml geniposidic acid showed obvious inhibition on SMC migration from the upper chamber (p?<?0.05).

Discussion and conclusion: The effects of geniposidic acid on protecting vascular endothelium and reversing plaque formation in an atherosclerotic model were demonstrated.     

蟾蜍卵母细胞发育过程中膜电位变化

作者对发育程度不同的蟾蜍卵母细胞膜电位测定观察到直径为0.2,1.0和1.6mm的卵母细胞膜电位分别为-6.0±2.0mv(n=13),-22±3.2my(n=13),-50±4.7my(n=10);有随着胞体增大膜电位逐渐超极化的趋势;然而卵母细胞成熟后,膜电位明显去极化。诱发成熟的和自发成熟的卵母细胞膜电位分别为-30.3±3.9mv(n=8),-37±8.4my(n=9)。这些变化的确切机制仍有待于进一步探讨。     

色氨酸对蟾蜍卵母细胞膜电位的影响

本文采用微最极细胞内记录方法观察了色氨酸对蟾蜍卵母细胞膜电位影响和时间效应和剂量效应,色氨酸对裸化卵母细胞膜电位的影响;色氨酸对卵母细胞膜电位起极化作用的离子关系等,其结果是以1mmol/L 色氨酸处理卵母细胞0~-1h 膜电位无明显变化,2h 后膜电位值便随培养时间延长而逐渐升高(超极化),随着色氨酸浓度升高其膜电位也逐渐升高。色氨酸对裸化卵母细胞膜电位有轻度超极化作用,但与对照组无明显差异(p>0.05):色氨酸虽然能使卵细胞膜电位明显超极化,但对细胞膜钠离子转运系数(TNa~ )无明显影响,而且膜电位变化与 TNa~ 变化无相关性。     

Ethanol hyperpolarizes mitochondrial membrane potential and increases mitochondrial fraction in cultured mouse myocardial cells

Cultured mouse heart-derived myocardial and non-muscle cells were exposed to ethanol, stained with cell-permeant fluorescent vital probes, JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolyl-carbocyanine iodide) and oxidation-sensitive dihydrorhodamine 123, and analyzed by flow cytometry to elucidate ethanol-induced time-wise alterations in the mitochondrial membrane potential (ΔΨm) and the production of reactive oxygen species (ROS). Ethanol (50 and 200 mM) not only hyperpolarized ΔΨm of both types of cells but also dose-dependently increased ROS production at 24 h, although a 200-mM dose reduced the production until 3 h. These cell pathophysiological reactions suggest the depression of mitochondrial ATPase and mitochondrial respiratory chain. However, differences between these cells appeared after a 24-h exposure to 200 mM ethanol: the increase in ROS production was approximately twice as large for myocardial cells as for non-muscle cells; and the side-scatter parameter of light scattering significantly increased for myocardial cells, but not for non-muscle cells. All these myocyte-specific alterations indicate an increase in the mitochondrial fraction in a cell. This reaction might be a countermeasure against ethanol-induced dysfunction of mitochondrial respiration that is needed to meet the energy requirements of spontaneous myocardial contractions.     

Effect of glutamate receptor ligands on mitochondrial membrane potential in rat dissociated cerebellar cells

The effect of three different glutamate receptor ligands on mitochondrial membrane potential has been studied in rat pup dissociated cerebellar cells by measuring rhodamine 123 fluorescence. L-glutamate, NMDA (N-methyl-D-aspartate) and kainate (from 10^–8 to 10^–3 M) decreased in a concentration-dependent manner the mitochondrial membrane potential with EC₅₀ values of 6.7±1.7, 3.8±0.5, and 37.4±14 M, respectively.Dizocilpine ((+)MK 801) was able to inhibit the NMDA- and L-glutamate-induced decrease in rhodamine 123 fluorescence, while kainate-induced fluorescence-decreases were unaffected. However, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) totally prevented the effect of kainate on mitochondrial membrane potential, but failed to block the L-glutamate effect. It is concluded that, in our cell preparation, L-glutamate exerts its action mainly through NMDA-subtype receptors, and that Ca²⁺ and Na⁺ entry through ionotropic glutamate receptors could be responsible for an impairment of mitochondrial membrane potential.     

用定量细胞学方法测试甲状腺滤泡上皮细胞高度的临床意义

本研究采用图像分析定量细胞学测定法,对甲状腺机能异常 者进行了甲腺状泡上皮细胞高度测定,实验分为无危象前兆征的结甲组,有危象前铛征的结甲组、甲亢组,结果发现有危象前兆征的结甲组中的滤泡上皮细胞高度有16例高于无危象前兆征的结甲组的滤泡上皮细胞高度,因此,测定甲状腺滤泡上皮细胞高度对甲状腺机能异常合理诊治,明确甲状腺机能状态,为今后手术决策,准确评估预后,有重重要的临床意义。     

孕酮对卵母细胞膜电位影响的分析

本实验采用微电极细胞内记录方法,观察了不同浓度孕酮及不同作用时间对卵母细胞膜电位影响。发现孕酮具有致充分生长的蟾蜍卵细胞膜电位去极化的作用,其去极化效应有时间与剂量依赖性关系。     

Involvement of cytotoxicity and variation of the mitochondrial membrane potential induced by hybrid agent

The pyrrolo[2,1‐c][1,4]benzodiazepine (PBD) derivative DC‐81 is a potent antitumor antibiotic produced by Streptomyces species. The marked cytotoxic potential of this drug may be the result of its interaction with DNA. Because DC‐81 only recognizes three DNA base pairs this has precluded its clinical utility. Combination of DC‐81 with an indole carboxylate moiety was a hybrid designed to have much higher sequence selectivity in DNA Interactivity. In this paper, the association between cytotoxicity and the changes of mitochondria membrane potential ΔΨ_mt after exposing human melanoma cell line A375 to the hybrid agent was examined using MTS cell proliferation assay and flow cytometry using the fluorochrome rhodamine 123. Our results indicated that the hybrid induced cytotoxity and a significant reduction in ΔΨ_mt of A375 cells. We suggest that the hybrid agent is a potent inducer of cell apoptosis in A375 cells. Drug Dev. Res. 61: 1–5, 2004. © 2004 Wiley‐Liss, Inc.