心解1号对实验性心律失常的影响

大鼠静脉恒速推注与头碱(Aco)诱发大鼠心律失常法和结扎犬冠状动脉前降支造成动物急性心肌缺血诱发心律失常法,以ECG改变为指标观察心解1号对实验性心律失常的影响.结果显示心解1号能显著提高Aco诱发大鼠心律失常的阈值.心0.9mg/kg组与同毒水平(1/10LD50)普鲁卡固胺(PA)比较,两药组Aco对室性早博(VE)诱发剂量相当,而对室性过速(VT)、室性纤颤(VF)、死亡(CA)等指标,心解1号对Aco诱发剂量分别较PA组提高30.8ug/kg、40.4ug/kg、42.0ug/kg;心解1号能有效降低犬心肌缺血时VE、VT的发生,给药前后结果比较P<0.001.实验表明心解1号具有较好的抗Aco所致心律失常作用及改善犬心肌缺血性心律失常作用.     

高性激素血症对大鼠心肌梗塞范围及左心室舒缩性能的影响

预先给雄性大鼠肌注丙酸睾丸素(TP)或苯甲酸雌二醇(E_2B)造成高雄或高雌性激素血症,3周后结扎左冠状动脉制作心肌梗塞(MI)模型,MI后继续给药以维持血浆性激素水平,观察性激素对大鼠心肌梗塞范围(IS)及左心室舒缩性能的影响。结果表明;(1)TP及E_2B对IS均无明显影响;(2)大剂量TP可显著改善MI大鼠的左室舒缩性能,而E_2B似对心功能有不利影响;(3)其左室舒缩性能指标(包括±dp/dt max及LVSP)均与血浆性激素水平呈显著的直线相关关系,即与睾酮(T)含量呈正相关,与雌二醇(E_2)含量及E_2/T的比值呈负相关。提示性激素在MI病理生理过程中确起一定作用。     

男性冠心病患者性激素与脂蛋白（a）关系的初步探讨

目的：探讨男性冠心病患者性激素与血脂的关系。方法：分别用放射免疫法及ＥＬＩＳＡ法测定血清性激素及脂蛋白（ａ）［Ｌｐ（ａ）］水平，用酶法及透射比浊法测定血胆固醇及载脂蛋白浓度。结果：冠心病组血清睾酮（Ｔ）、高密度脂蛋白胆固醇（ＨＤＬ－Ｃ）明显低于正常对照组（Ｐ＜００１），血清雌二醇与睾酮比率、Ｌｐ（ａ）明显高于正常对照组（Ｐ＜００１），而血清雌二醇（Ｅ２）、载脂蛋白及血浆总胆固醇（ＴＣ）两组间无显著差异（Ｐ＞００５）。冠心病组血清Ｔ水平与ＨＤＬ－Ｃ水平呈显著正相关（Ｐ＜００１），与Ｌｐ（ａ）水平呈显著负相关（Ｐ＜００５）；血清Ｅ２水平与Ｌｐ（ａ）水平无相关性。结论：睾酮下降为主的性激素失衡是男性冠心病的致病因子。     

血浆性激素对心肌梗塞大鼠左室功能影响的实验研究

本文观察了实验性心肌梗塞(MI)大鼠血浆性激素与左室功能变化的关系。发现,去睾丸鼠血浆睾酮(T)明显降低(P<0.025),同时心率(HR)变慢,左室射血时间(LVET)延长,左室收缩压峰值降低,±dp/dt_(max)、V_(CE50)都明显降低(P<0.01,<0.001,≈0.05),T值增大(P<0.002),心肌纤维直径(MFD)减小(P<0.01);去卵巢鼠血浆T轻度升高,同时HR增快,T值缩短(P<0.025)MFD增大(P<0.005),而心肌收缩性能无显著变化。MI后,雄鼠血浆T明显降低,E_2/T比值增大,雌鼠血浆T升高,E_2/T比值减小;同时雄鼠的±dp/dt_(max)、V_(CE50)显著降低,T值明显延长等均较雌鼠为著,而雌鼠的MFD增大的程度较雄鼠明显。这说明MI后,血浆T的改变与左室心肌舒缩性能的变化和心肌肥大的建立密切相关。因此,推测T在维持和调节心肌舒缩性能上起着重要作用。     

性激素与肝肿瘤

雌激素和雄激素可影响肝肿瘤的发生和发展，在实验动物和人体正常肝及肝肿瘤内可测到雌激素受体和（或）雄激素受体。性激素受体可能是性激素促进肝肿瘤发生、发展和肝肿瘤抗激素治疗的介导物。     

脑卒中与心律失常的关系

目的 探讨脑卒中与心律失常的关系。方法 对157例脑卒中患者的心电图及临床资料分析。结果 脑卒中的心律失常检出率为56．6％，出血性卒中心律失常发生率高于缺血性卒中（P〈0．05）。结论 脑卒中病灶位于丘脑者及有高血压伴昏迷症状者心律失常发生率高，心律失常与预后有关。     

一种大鼠心律失常自动检测分析系统

本大鼠心律失常检测分析系统适于８０×８６系列微机，具有数据采集（定时器中断方式采样）、实时显示、波形测量、文件操作、自动分析、数据压缩、打印输出及在线帮助等功能。界面采用多导记录仪模式，中文下拉式菜单，鼠标驱动。用“差分平方法”自适应阈值检取Ｒ波，“主波夹角法”提取波宽，采用“时间窗口低阈值回扫”、设置“反拗期”和二次综合判断等方法，使Ｒ波检出正确率在９９％以上。对心律失常自动分类统计（包括早搏、停搏、室速、室颤等项），结果打印输出或存盘供其他统计程序调用。动物实验证实，其诊断正确率为９９．２％。     

甘草黄酮抗实验性心律失常的作用

甘草黄酮（ｇｌｙｃｙｒｒｈｅｉｃｂｒｓｓ．ＧＢ）２ｍｇ／ｋｇ能明显对抗乌头碱２０μｇ／ｋｇ，ＢａＣｌ２２ｍｇ／ｋｇ和结扎左冠状动脉前降支诱发大鼠的室性心律失常。甘草黄酮也能明显对抗ＣａＣｌ２－Ａｃｈ（ＣａＣｌ２０．６％＋Ａｃｈ０．００２５％）混合液诱发小鼠心房纤颤或扑动，ＧＢ对大鼠心电图实验，证明了有负性频率作用，负性传导作用，这些作用可能是ＧＢ抗心律失常作用的药理基础。     

类风湿性关节炎患者白细胞性激素受体和性激素改变的研究

采用放射配体结合法检测42例类风湿性关节炎患者外周血白细胞雄激素受体（AR）和雌激素受体（ER）水平；同时用放射免疫分析检测其血浆中的睾酮（T）和雌二醇（E2）。类风关患者性激素无明显改变，而外周血白细胞ER明显升高及AR明显降低。提示性激素受体改变与类风关发病学有一定关系。     

Genetic polymorphisms, hormone levels, and hot flashes in midlife women

OBJECTIVE: Hot flashes disrupt the lives of millions of women each year. Although hot flashes are a public health concern, little is known about risk factors that predispose women to hot flashes. Thus, the objective of this study was to examine whether sex steroid hormone levels and genetic polymorphisms in hormone biosynthesis and degradation enzymes are associated with the risk of hot flashes. METHODS: In a cross-sectional study design, midlife women aged 45-54 years (n=639) were recruited from Baltimore and its surrounding counties. Participants completed a questionnaire and donated a blood sample for steroid hormone analysis and genotyping. The associations between genetic polymorphisms and hormone levels, as well as the associations between genetic polymorphisms, hormone levels, and hot flashes were examined using statistical models. RESULTS: A polymorphism in CYP1B1 was associated with lower dehydroepiandrosterone-sulfate (DHEA-S) and progesterone levels, while a polymorphism in CYP19 (aromatase) was associated with higher testosterone and DHEA-S levels. Lower progesterone and sex hormone binding globulin levels, lower free estradiol index, and a higher ratio of total androgens to total estrogens were associated with the experiencing of hot flashes. A polymorphism in CYP1B1 and a polymorphism in 3betaHSD were both associated with hot flashes. CONCLUSION: Some genetic polymorphisms may be associated with altered levels of hormones in midlife women. Further, selected genetic polymorphisms and altered hormone levels may be associated with the risk of hot flashes in midlife women.     

性激素水平及主动脉雌激素受体的变化对鹌鹑动脉粥样硬化形成的影响

本文探讨了高血脂、性激素及其受体与动脉粥样硬化病变之间的关系。结果表明：高血脂可影响性腺细胞内3－β-羟基甾体脱氢酶含量及脂质贮存量，导致动物体内性激素环境异常及动脉壁细胞雌激素受体水平改变。提示高脂血症作为AS的始动因子之一，不仅影响动脉壁细胞的脂质代谢，还可诱发机体性激素水平紊乱，从而影响动脉壁细胞雌激素受体的表达，使之生物学行为改变，促进AS的发生发展。     

性激素对大鼠胸腺上皮细胞分泌胸腺素α1的影响

胸腺产生胸腺素等肽类激素，控制胸腺依赖淋巴细胞的发育并参与免疫调节过程。本实验用原位杂交法和高效液相色谱分析法研究了性激素（雌二醇和双氢睾酮）对培养胸腺上皮细胞（ＴＥＣ）胸腺素α１（ＴＭＡ１）生成的影响。结果如下：（１）ＴＭＡ１－ｍＲＮＡ活性产物成群分布于ＴＥＣ胞浆中；（２）雌二醇浓度大于３×１０－９ｍｏｌ／Ｌ使ＴＭＡ１－ｍＲＮＡ活性细胞数目明显减少；（３）双氢睾酮处理不影响ＴＭＡ１－ｍＲＮＡ活性细胞的数量；（４）雌二醇或双氢睾酮均使ＴＥＣ生成ＴＭＡ１明显减少。结果表明：性激素特别是雌二醇通过影响胸腺素的生成而调节免疫功能。     

Effects of neonatal exposure to progesterone on sexual behavior of male and female rats

Lactating females received daily injections of progesterone or oil, and their offspring were gonadectomized in adulthood and tested for both musculine and feminine sexual behavior elicited by estrogen, progesterone, and testosterone regimens. Male offspring of progesterone treated females exhibited significant impairment of masculine behavior elicited by both estrogen and testosterone. Latency and frequency of mounts and intromissions of those males which did engage in sexual behavior were not significantly different for the two groups. There were nonsignificant trends toward demasculinization of progesterone treated females and feminization of progesterone treated males. Progesterone administered to estrogen primed males failed to facilitate lordosis. There were no progesterone related differences in body weight at any time nor in testis or accessory organ weights of males. The results of this experiment confirm our previous finding of reduced sexual competence of intact male rats exposed neonatally to moderately increased levels of progesterone, and indicate that this effect is not the result of diminished adult hormone levels. Neonatal exposure to progesterone appears to have minimal or no effect on feminine sexual behavior of rats.     

男性类风湿关节炎患者血浆催乳素水平的变化及其临床意义

目的：为探讨男性类风湿关节炎（ＲＡ）患者血浆催乳素（ＰＲＬ）水平的变化有临床意义。方法：用放射免疫法对６２例男性ＲＡ患者进行了血浆ＰＲＬ、雄激素睾酮（Ｔ）Ｔ、去氢表雄固酮（ＤＨＥＡ－Ｓ）水平测定及其多聚酶链反应（ＰＣＲ）作了ＨＬＡ＿ＤＲ４筛选,并与相庆的临床和实验室指标作对比分析。结果：ＲＡ患者血冰ＰＲＬ水平与正常人比较无显著差异,但在中度以上活动性的患者或ＨＬＡ＿ＤＲ４阳性患者中的血浆ＰＲＬ水平     

Changes in sex hormones and offspring sex ratio following gasoline exposure in male rats

An experimental study was performed to determine the effect of gasoline inhalation on sex hormones and offspring sex ratio in male rats. Twenty two Sprague–Dawley adult male rats were divided into two groups. In the first group (n = 11), animals were exposed to gasoline vapor for 6 h daily for 30 consecutive days. The second group served as control without gasoline exposure. In the end of the study, sex hormones including total testosterone, luteinizing hormone and, follicle-stimulating hormone were determined using radioimmunoassay methods. Then, animals in both groups were mated with healthy unexposed female rats and sex of offspring was determined after birth. Results showed that the level of all sex hormones was significantly decreased in test group compared with control ones (P < 0.02). Offspring sex ratio was 0.39 (38/96) in the test group, significantly lower than 0.54 (60/101) in the control group (P = 0.03). The potential of manipulating offspring sex ratio through a simple, safe, and practical method would be an interesting subject. The results of the present study could provide a baseline for future research on this subject.     

Effects of early sex steroid hormone treatment on courtship behavior and sexual attractivity in the red-sided garter snake, Thamnophis sirtalis parietalis

Previous research has shown that exogenous androgen fails to elicit courtship (chin-rubbing) behavior in adult male or female red-sided garter snakes (Thamnophis sirtalis parietalis). However, gonadectomized and intact newborn male and female red-sided garter snakes given silastic capsules containing testosterone exhibit chin-rubbing behavior; gonadectomized and untreated animals do not show this behavior. Both males and females also exhibit chin-rubbing behavior when treated with testosterone as yearlings. Hibernation stimulates chin-rubbing behavior only in males that have received androgen treatment as a neonate or as a yearling. Previous research has also shown that adult females, but not adult males, are courted if given estrogen treatment. Both newborn males and females will elicit chin-rubbing behavior from adult males if given estrogen treatment, indicating production and release of an attractiveness pheromone characteristic of adult females. Male red-sided garter snake breed for the first time on emergence from their second hibernation, whereas females probably do not breed until their third year of life. These data suggest that in the red-sided garter snake, a species that exhibits a dissociated reproductive tactic, sex steroid hormones act to organize central nervous system mechanisms subserving courtship behavior such that temperature, and not hormonal, fluctuations activate sexual behavior in the adult organism.