古墨膏抗炎作用的实验研究

为研究古墨膏的抗炎作用,采用小鼠耳肿胀模型、大鼠足跖肿胀模型及小鼠腹腔毛细血管通透性增高模型观察古墨膏的抗炎作用。结果显示,古墨膏能显著抑制二甲苯所致小鼠耳肿胀,明显减轻鸡蛋清致炎大鼠足跖肿胀度,并具有抑制醋酸引起的小鼠腹腔毛细血管通透性增加作用。结果表明,古墨膏对动物炎症模型具有明显的抗炎作用。     

翁沥通胶囊的药理作用研究

目的 研究翁沥通胶囊的抗炎、镇痛和改善微循环作用。方法 采用醋酸扭体模型和热板疼痛模型观察镇痛作用；采用小鼠耳廓微循环系统观察对微循环的影响：采用大鼠无菌性前列腺炎症模型和慢性前列腺炎症模型观察抗炎作用。结果 翁沥通胶囊可明显抑制小鼠招体次数，延长小鼠热板疼痛潜伏期；扩张小鼠耳廓微动脉和微静脉；抑制人鼠无菌性前列腺炎症和慢性前列腺炎症。结论 翁沥通胶囊具有镇痛、改善微循环和抗前列腺炎作用。     

急性子提取物抗炎镇痛作用的实验研究

目的探讨中药急性子的抗炎镇痛效果，为临床合理应用提供理论依据。方法采用二甲苯致小鼠耳肿胀、冰醋酸致小鼠扭体反应、小鼠热板法、蛋清致大鼠足肿胀和肉芽肿实验观察急性子提取物的抗炎镇痛效果。结果与模型对照组比较，急性子水提物中、大剂量组小鼠扭体次数明显减少（P＜0.05），急性子水提物中、大剂量组大鼠足肿胀减轻。中、大剂量急性子水提物对小鼠耳肿胀无效，其中大剂量可延长60 min（P＜0.01）和90 min（P＜0.05）小鼠疼痛阈值；急性子醇提取物大剂量可显著减少小鼠扭体次数（P＜0.01），减轻小鼠耳肿胀程度（P＜0.05），明显延长30 min疼痛阈值（P＜0.01），减轻大鼠肿胀程度；急性子醇提物大剂量可延长60 min疼痛阈值（P＜0.01）。急性子提取物对大鼠肉芽肿无抑制作用。结论急性子提取物有一定的镇痛效果和抑制急性炎症作用。     

红元胶囊抗炎镇痛作用的药效学实验观察

目的:研究红元胶囊的抗炎镇痛效果.方法:采用大鼠棉球肉芽肿试验、二甲苯致小鼠耳廓肿胀度实验、甲醛致小鼠疼痛反应实验、0.6%醋酸致小鼠疼痛反应实验.结果:红元胶囊经口服给药可抑制大鼠棉球肉芽肿和小鼠二甲苯所致耳廓肿胀,可降低甲醛致小鼠疼痛反应分值,对0.6%醋酸所致小鼠疼痛有明显镇痛作用.结论:红元胶囊有抗炎镇痛作用.     

木芙蓉叶有效组分抗非特异性炎症的实验研究

目的观察木芙蓉叶有效组分(MFR-C)的抗非特异性炎症作用.方法通过大鼠鹿角菜致肿实验、大鼠蛋清致肿实验、小鼠腹腔毛细血管通透性实验和小鼠耳肿胀实验,对木芙蓉叶有效组分抗非特异性炎症的作用进行评定.结果 MFR-C和盐酸青藤碱对鹿角菜及蛋清所致的大鼠足肿胀具有良好的抑制作用,与空白对照组相比差异有显著性(P＜0.05);与空白组相比,MFR-C对乙酸引起的小鼠腹腔毛细血管通透性也具有良好的抑制作用,两者之间差异有显著性意义(P＜0.05);小鼠耳肿胀实验显示MFR-C有抑制二甲苯造成的非特异性耳肿胀作用,与空白组相比P＜0.05,与阳性对照组(盐酸青藤碱)相比有同样的抗炎消肿作用.结论木芙蓉叶有效组分(MFR-C)对鹿角菜、蛋清、乙酸及二甲苯引起的非特异性炎症具有良好的抑制作用.     

hCG调节先天性尿道下裂小鼠阴茎皮肤EGF含量的实验研究

目的:通过建立先天性尿道下裂动物模型,探讨其病因,研究外源性hCG对先天性尿道下裂小鼠阴茎皮肤表皮生长因子(EGF)的影响。方法:建立先天性尿道下裂动物模型。随机选取出生后3周龄正常雄性仔鼠50只腹腔内注射生理盐水1ml作为正常对照组,尿道下裂仔鼠50只,其中10只腹腔内注射生理盐水1ml作为试验对照组,其余40只分为4组,每组10只,腹腔内分别注射hCG(50、100、150、200IU)1次/d,共7d。ELISA法定量检测正常对照组和试验组小鼠阴茎皮肤组织和血清中EGF的浓度。结果:先天性尿道下裂小鼠阴茎皮肤组织EGF的浓度明显低于正常对照组(P<0.05)。hCG注射150、200IU组与尿道下裂生理盐水注射组和hCG注射50、100IU组间小鼠阴茎皮肤组织中EGF的浓度差异有显著性(P<0.05),hCG注射50、100IU组和尿道下裂生理盐水注射组与正常对照组间阴茎皮肤组织中EGF的浓度差异有显著性(P<0.05),小鼠血清中EGF的浓度各组间组内差异无显著性。结论:雄激素受体拮抗剂类药物可造成尿道下裂的发生,小鼠阴茎皮肤组织EGF浓度降低可能与尿道下裂的病因有关。外源性hCG在150～200IU剂量范围内可刺激先天性尿道下裂小鼠阴茎组织EGF浓度的增加。     

右美托咪定两种给药方法在急性腹膜炎小鼠局部组织的抗炎镇痛作用中的比较

目的通过观察比较右美托咪定静脉注射和腹腔注射两种给药方法在急性腹膜炎模型小鼠炎性内脏痛的镇痛及抗炎作用。方法 SPF级健康成年雄性小鼠60只,体重24~28 g,采用随机数字表法分为五组,每组12只:空白对照组(CK组)、急性腹膜炎模型组(VP组)、右美托咪定静脉注射组(DEX-V组)、右美托咪定腹腔注射组(DEX-P组)、右美托咪定+甲基牛扁碱组(DEX-M组)。VP组、DEX-V组、DEX-P组DEX-M组腹腔注射0.9%乙酸溶液0.1 ml/10 g建立急性腹膜炎模型,CK组腹腔注射等容量生理盐水。于造模前15 min,VP组、DEX-P组分别经腹腔注射生理盐水、右美托咪定10μg/kg,DEX-V组经尾静脉注射右美托咪定10μg/kg,DEX-M组经腹腔注射右美托咪定10μg/kg和α7nACh受体特异性拮抗剂甲基牛扁碱2.4μg/g。观察并记录小鼠急性腹膜炎模型建立后2 h内的镇静情况、扭体反应和内脏痛指数(VPI评分);建模6 h后取材并采用ELISA法检测小鼠血清和腹膜组织匀浆中IL-6、TNF-α的浓度;取小鼠腹腔注射部位壁层腹膜组织,在光镜下观察其水肿程度和中性粒细胞浸润情况。结果与CK组比较,VP组、DEX-V组、DEX-P组和DEX-M组均出现扭体反应,给药后15、30、45、60、75、90、105、120 min时VPI评分明显升高(P<0.05),血清和腹膜组织匀浆的IL-6和TNF-α浓度均明显升高(P<0.05),腹膜组织出现不同程度水肿及中性粒细胞浸润;与VP组比较,DEX-V组和DEX-P组镇静效果较好,给药后30、45、60、75 min时VPI评分明显降低(P<0.05),血清和腹膜组织匀浆的IL-6和TNF-α浓度均明显降低(P<0.05),腹膜组织水肿减轻、中性粒细胞浸润减少;与DEX-P组比较,DEX-V组腹膜组织匀浆中的IL-6和TNF-α浓度明显升高(P<0.05);DEX-M组镇静情况、给药后45、60、75、90 min时VPI评分、血清和腹膜组织匀浆的IL-6和TNF-α浓度均明显升高(P<0.05),局部腹膜组织水肿明显加重,中性粒细胞浸润增多。结论右美托咪定局部腹腔注射可以有效降低急性腹膜炎小鼠的内脏痛指数,对局部腹膜组织有抗炎作用且效果优于静脉注射,其抗炎机制可能部分与激活a7nACh受体引导的胆碱能抗炎通路有关。     

N-甲基-D-天门冬氨酸受体与吸入麻醉药对小鼠催眠、镇痛作用的关系

目的探讨N．甲基．D．天门冬氨酸(NMDA)受体与安氟醚、异氟醚、七氟醚对小鼠催眠及镇痛作用的关系。方法实验动物选用昆明种小鼠,雌雄不拘。第一部分为催眠实验,120只小鼠随机分成安氟醚、异氟醚、七氟醚三组,分别腹腔注射安氟醚2 ITIl·kg～、异氟醚1．2IrIl·kg～、七氟醚5 rnl·奴～,每组又分成人工脑脊液(aCSF‘)、NMDA 25、NMDA 50及NMDA 75亚组,每亚组10只,各组翻正反射消失后立即侧脑室注射aCSF、NDMA25、50、75 mg 10 m,记录翻正反射恢复(RT)时间。第二部分为镇痛实验,160只小鼠随机分成对照、安氟醚、异氟醚、七氟醚组,对照组不给予任何处理,安氟醚、异氟醚、七氟醚组分别皮下注射安氟醚1．5 llll·kg一、异氟醚0．8 Jnl·kg一、七氟醚4．5 llll·kg～,各组又分成 aCSF、NMDA 2．5、NMDA5及NMDA 10亚组,每亚组10只,各组注药后10 min鞘内注射aCSF或2．5、5、 10 ng NMDA lO出,1 min后腹腔注射O．6％冰醋酸,记录扭体次数。结果催眠实验中,安氟醚、异氟醚、七氟醚组的亚组间比较RT差异无统计学意义(P>0．05)。镇痛实验中,与aCSF亚组比较,对照组的NMDA 2．5亚组、NMDA 5亚组、NMDA 10亚组扭体次数差异无统计学意义,安氟醚、异氟醚、七氟醚组的NMDA 5 ng亚组、NMDA 10 ng亚组扭体次数增加(P相似文献   

金疮止血消炎药抑菌抗炎作用实验研究

目的:研究金疮止血消炎药的抑菌、抗炎作用。方法:用肉汤稀释法测定金疮止血消炎药对呼吸道常见致病菌的最低抑菌浓度(MI C);用蛋清诱发大鼠足肿胀急性炎症模型;大鼠棉球肉芽肿慢性炎症模型;二甲苯诱发小鼠耳肿胀和小鼠腹腔毛细血管通透性试验;研究金疮止血消炎药的抗炎作用。结果:金疮止血消炎药具有体外抑菌作用,对引起呼吸道感染的9种常见病原菌和致病菌均有不同程度的抑制作用;金疮止血消炎药具有抗炎作用,对大鼠蛋清性足肿胀、大鼠棉球肉芽肿、二甲苯致小鼠耳廓肿胀及小鼠腹腔毛细血管通透性试验均有不同程度的抑制作用。结论:本研究结果提示金疮止血消炎药具有抑菌、抗炎作用,为金疮止血消炎药临床用于外伤止血抗炎、溃疡、流感、上呼吸道感染、咽喉肿痛等症提供了实验研究依据。     

消痔栓抗炎消肿、镇痛作用的实验研究

通过动物实验研究消痔栓抗炎消肿、镇痛的作用机制。采用小鼠冰醋酸致痛观察消痔栓的镇痛作用,同时测定新鲜鸡蛋清致小鼠后足跖肿胀炎性组织中PGE2、NO、Pr含量。结果显示,消痔栓能显著抑制冰醋酸致痛小鼠扭体次数,明显降低新鲜鸡蛋清致小鼠后足跖肿胀炎性组织中PGE2、NO、Pr含量。结果表明,消痔栓的抗炎消肿、镇痛机制之一可能与降低炎性组织中的PGE2、NO、Pr的含量有关。     

复方苦参凝胶节育与抗炎的体内实验研究

目的:研究复方苦参凝胶体内节育及抗炎的效果。方法:①节育实验:将60只雌性SD大鼠随机分为生理盐水组,空白凝胶组,复方苦参凝胶低(0.05 g生药/g)、中(0.10 g生药/g)、高剂量(0.15 g生药/g)组以及阳性对照(4%壬苯醇醚凝胶)组,每组10只。按上述分组雌鼠阴道内给予相应药物各200μl,然后雌雄大鼠按1∶1合笼,进行交配。在交配成功后的第12天剖腹观察雌鼠孕珠数,并计算避孕率;②抗炎实验:采用小鼠耳廓双面均匀涂抹二甲苯的致炎模型,将60只昆明种小鼠随机分为6组,分组同前,计算小鼠单侧耳的肿胀度及肿胀抑制率。结果:复方苦参凝胶高剂量组能显著抑制雌鼠的孕珠数,其孕珠数为0.00±0.00,显著低于生理盐水组(11.00±2.00,P<0.05),而与阳性对照组(0.00±0.00)比较无统计学意义(P>0.05),其避孕率为100%;在小鼠耳廓二甲苯致炎模型中,高剂量组同样能显著抑制其肿胀程度,其平均肿胀程度为10.17±2.56,显著低于生理盐水组(21.32±3.17,P<0.01),而与阳性对照组比较无统计学意义(8.53±1.89,P>0.05),其肿胀抑制率为52.3%。结论:复方苦参凝胶有较好的节育及抗炎作用,值得进一步开发并应用于临床。     

耳穴压籽疗法在痔术后疼痛护理中的应用

为观察耳穴压籽疗法在痔术后疼痛护理中的应用效果,将行痔手术患者140例随机分为对照组和观察组,每组70例.对照组患者术后采用常规疼痛护理,观察组患者在此基础上采用耳穴压籽疗法,比较2组患者的止痛效果.结果显示,观察组患者总有效率为94.28％,明显高于对照组的82.86％,比较差异有统计学意义(P<0.05).观察组患...     

CO2气腹对胃癌细胞SGC-7901裸鼠腹腔增殖和侵袭能力的影响

目的 通过体内模拟腹腔镜气腹环境,探讨CO2:气腹对裸鼠腹腔胃癌细胞增殖和侵袭转移能力的影响.方法 棵鼠随机分成3组:对照组(A组).行开腹探查;5 mmHg(1 mmHg=0.133 kPa)CO2气腹组(B组);8 mmHg CO2:气腹组(C组);每组9只.人胃癌细胞SGC-7901棵鼠腹腔注射后建立CO2气腹胃癌细胞裸鼠腹腔种植模型,气腹持续1 h.建模后每周秤体质量,观察各组裸鼠生长情况和腹腔成瘤情况.28 d后处死解部裸鼠,记录腹水量、腹腔各脏器、穿刺口和腹壁切口成瘤情况和肿瘤数,测量肿瘤最大径、总重量,并检测胃癌种植瘤组织中乙酰肝素酶(Hpa)mRNA的表达.结果 各组裸鼠成瘤率100%.各组裸鼠体质量、腹腔不同脏器成瘤率、腹水量差异均无统计学意义(P>0.05).肿瘤结节数:A组(7.4±2.5)个,B组(7.3±2.4)个,C组(7.5±2.1)个.肿瘤最大径:A组(7.0±2.2)mm,B组(6.9±2.0)nnn,C组(6.8±1.9)mm;肿瘤重量:A组(0.99±0.30)g.B组(0.87±0.20)g,C组(0.91±0.13)g.Hpa mRNA表达指数:A组0.41±0.07,B组0.45±0.06,C组0.43±0.09,各组间差异均无统计学意义(P>0.05).结论 在5 mmHg和8 mmHg气腹压力下,CO2:气腹不促进胃癌细胞裸鼠腹腔内增殖和侵袭转移,也未明显影响胃癌细胞成瘤组织Hpa mRNA的表达.     

出生后注射雌二醇诱导隐睾性不育小鼠模型的实验研究

目的:观察出生后注射雌二醇诱导小鼠隐睾模型的发生率及生精情况。方法:90只雄性新生Balb/C小鼠随机分为实验组(n=60)、溶剂对照组(n=20)和正常对照组(n=10)。实验组进一步随机分为4个亚组,分别于出生后3～28 d(4周组)、3～42 d(6周组)、3～56 d(8周组)、3～70 d(10周组)给予皮下注射17-β雌二醇(5μg/d)。观察停药后2周隐睾发生率及形态学变化。结果:实验组各亚组(4、6、8、10周组)停药后2周隐睾发生率分别为0%、26.7%、60%、60%。而对照组均无隐睾发生。在4周和6周组停药后均出现隐睾自行下降恢复生精的情况,连续注药8周后模型稳定,隐睾生精不恢复。结论:出生后连续注射雌二醇8周能够建立稳定的隐睾性不育小鼠模型。     

阴茎包埋对海绵体内勃起通路的影响

目的 观察阴茎包埋对海绵体内勃起通路的影响.方法 通过建立大鼠隐匿阴茎模型获得实验标本,分2、4、6个月组进行观测.各阶段中包括包埋组、假手术组和正常组.采用紫外分光光度计检测海绵体内一氧化氮合酶(NOS)的活性,免疫组织化学法显示雄激素受体(AR)在海绵体内的表达水平.结果 3个时间段内包埋组NOS活性分别为1.79、1.67、1.24 U/mg·prot,与正常组和对照组比较,酶活性降低随包埋时间延长逐渐显著(2个月组P>0.05,4个月组P<0.05,6个月组P<0.01),但包埋对各阶段大鼠阴茎海绵体内AR表达水平无影响.结论 阴茎包埋可直接影响勃起通路中最重要的神经递质一氧化氮合成的关键酶-NOS的活性来影响勃起通路,且这种影响与阴茎包埋时间正相关.     

The persisting analgesic effect of low-dose intravenous ketamine after spinal anaesthesia for caesarean section

BACKGROUND AND OBJECTIVES: To compare the analgesic effects of intrathecal fentanyl and low-dose intravenous ketamine as adjuvants to intrathecal bupivacaine for Caesarean section. METHODS: Ninety elective Caesarean section patients were randomized into three groups. Spinal anaesthesia was performed with 15 mg hyperbaric bupivacaine in all groups. Ketamine (0.15 mg kg(-1)) or an equal volume of normal saline was given intravenously immediately after initiating spinal anaesthesia in the ketamine and control group, respectively. In the fentanyl group, 10 microg fentanyl was added to the intrathecal bupivacaine. Arterial pressures, heart rate values, adverse effects, the time of first request for postoperative analgesia, visual analogue pain scores, total analgesic consumptions at 24 and 48 h were recorded in all patients. RESULTS: The time to first request for analgesia was significantly longer in the ketamine (197 min) and fentanyl (165 min) groups compared to the control group (144 min). Postoperative pain scores were significantly lower in the ketamine group than in both other groups. Although the analgesic requirements during first 24 h were significantly lower in the ketamine group, there was no significant difference between the groups during the following 24 h. CONCLUSION: Intravenous low-dose ketamine combined with intrathecal bupivacaine for Caesarean section provides longer postoperative analgesia and lower postoperative analgesic consumption than bupivacaine alone suggesting a pre-emptive effect.     

单侧骨皮质缺损对长管状骨生物力学的影响

目的探讨长管状骨骨干不同大小的单侧骨皮质缺损对长管状骨生物力学性质的影响。方法取76根三黄鸡胫骨,随机分7组(1.5mm组n=11,2.0mm组n=9,2.5mm组n=13,3.0mm组n=12,3.5mm组n=8,4.5mm组n=11,皮质完整组即正常对照组n=12),于后侧骨皮质造成直径分别为1.5～4.5mm的环形骨缺损,进行三点弯曲单一实验,皮质完整组作为正常组对照。结果缺损直径为1.5mm及2.0mm组鸡胫骨达到结构破坏时的最大载荷与正常组差异无统计学意义(P=0.824,0.865),2.5mm、3.0mm、3.5mm组的最大载荷较正常组降低14%左右,差异有统计学意义(P=0.015,0.010,0.021);4.5mm组较正常组降低23%左右,差异有统计学意义(P=0.001)。结论单侧皮质缺损直径在长管状骨外径的(22.6±1.4)%以内时,不影响其抗弯曲性能;当其超过长管状骨外径的(29.4±2.1)%时,将降低长管状骨的抗弯曲性能,但降低的幅度跟缺损大小并不完全呈正比。     

Non-steroidal anti-inflammatory drugs in treatment of postoperative pain after cardiac surgery

PURPOSE: Non-steroidal anti-inflammatory drugs (NSAIDs) are used as analgesic in postoperative pain to reduce opioid side effects, such as drowsiness and nausea. However, NSAIDs have not been used extensively in cardiac surgical patients due to the fear of untoward effects on gastric, renal, and coagulation parameters. This study will evaluate the efficacy and safety of three NSAIDs for pain control in CABG patients. METHODS: One hundred and twenty patients scheduled for elective CABG surgery were enrolled in randomized, double blind, controlled study. Standardized fast track cardiac anesthesia was used. One dose of drug (75 mg diclofenac, 100 mg ketoprofen, 100 mg indomethacin, or placebo) was given pr one hour before tracheal extubation and a second dose 12 hr later. Pain was treated with morphine iv and acetaminophen po. Visual analogue pain scores were recorded at baseline, 3, 6, 12 and 24 hr after the first dose of drug. RESULTS: There were no differences among the groups in pain scores. Only patients who received diclofenac required less morphine than patients in the control group (P < 0.05). When the total amounts of pain medications were computed to morphine equivalents, only patients in the diclofenac group received less pain medications than the placebo group (P < 0.05). Proportion of patients with postoperative increase of creatinine level (20% and over) did not differ between placebo and drug groups. CONCLUSION: Non-steroidal anti-inflammatory drugs may be used for analgesia management post CABG surgery in selected patients. Diclofenac appears to have the best analgesic effects by reducing the morphine and other analgesic requirement postoperatively.     

Is preoperative ketorolac a useful adjunct to regional anesthesia for inguinal herniorrhaphy?

Background: Nonsteroidal antiinflammatory drugs (NSAIDs) have become a popular component of analgesia regimens, particularly in combination with narcotics. We questioned whether there might also be a place for their use in conjunction with regional anesthesia and whether there was a preferable route for NSAID administration. Methods: Ilioinguinal and field blocks were performed pre-operatively on seventy patients undergoing outpatient inguinal hernia repair. Patients were divided into a control group who received no ketorolac and four study groups who received a preoperative dose of 30 mg ketorolac by one of the following routes: IV, IM, PO, or intrawound (IW). The ketorolac in the IW group was mixed in the syringe with the local anesthetic used for the field block. IV and IM groups also received ketorolac at the time of the preoperative regional anesthesia and the PO group received the dose at least one hour preoperatively. All patients received a similar general anesthetic intraoperatively. Results: Postoperative pain scores and analgesic requirements were lowest for the IM, IV, and IW groups. Pain scores and analgesic requirements for the PO group were less than for the control group but more than for the other three groups. Analgesic efficacy therefore ranked: IM = IV = IW>PO>Control. Though no differences were found between groups in the time to discharge from the recovery room, the ease of nursing care paralleled the findings for pain scores and analgesia requirements. Conclusion: Beyond the analgesia provided by the regional anesthesia of the ilioinguinal and field blocks, the preoperative use of ketorolac further reduced postoperative pain scores and the need for additional postoperative analgesic medication. Comparable outcomes for the IV, IM, and IW groups indicate the lack of any benefit to concentrating the non-steroidal anti-inflammatory drug at the wound (IW) or to achieving high blood levels rapidly (IV). In conclusion, ketorolac is a useful supplement to ilioinguinal plus field block regional anesthesia for hernia surgery and is most effective administered parenterally.     

不同剂量己烯雌酚对新生小鼠睾丸表皮生长因子及受体的影响

目的 观察不同剂量己烯雌酚(DES)对新生小鼠睾丸组织中表皮生长因子(EGF)及其受体(EGFR)的影响,探讨DES对睾丸发育影响的机制.方法 建立小鼠DES模型.将72只怀孕的雌性昆明小鼠随机分成3组:正常组、对照组及实验组1～4(DES 10、25、50、100 μg/kg).采用免疫组织化学方法检测各组新生小鼠睾丸组织中EGF、EGFR的表达.结果 EGF和EGFR主要表达在新生小鼠睾丸的间质细胞.实验组1～4中EGF阳性细胞的累积吸光度值(IA)分别为75.43±1. 42、52.22±5.67、13.75±3.14、6.38±3.20,显著低于正常组及对照组中阳性细胞的IA值433.88±11.64、23.44±4.70;实验组EGFR阳性细胞的IA值分别为198.16±34.35、138.00±12.04、46.03±6.74、27.22±5.52,显著低于正常组及对照组中阳性细胞的IA值804.74±22.52、800.03±21.96.两者在正常、不同剂量DES实验组的两两比较差异有统计学意义(P＜0.05).随DES剂量增加,EGF和EGFR表达减弱,各组间差异有统计学意义(P＜0.05).EGF与EGFR呈强正线性相关(r=0.750,P＜0.01).结论 不同剂量DES对新生小鼠睾丸组织中EGF和EGFR表达强度均有影响,可能是影响睾丸发育机制之一.

Abstract：

Objective To investigate the effects of prenatal exposure to diethylstilbestrol (DES)with different dosages on epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in offspring mice testis, and the possible mechanism. Methods DES model was induced in mice by DES.Female Kungmiag mice were randomly divided into normal group, control group and experimental groups 1-4 ( DES 10, 25, 50, 100 μg/kg). Immunohistochemistry was applied to detect the expression of EGF and EGFR in the testicular tissue in each group. Results EGF and EGFR were expressed mainly in sffspring mice testis Leydig cells. The cumulative absorbance ( IA ) values of EGF positive cells in experimental groups 1-4 were 75. 43 ± 1.42, 52. 22 ± 5.67, 13.75 ± 3. 14, and 6. 38 ± 3.20 respectively, which were significantly lower than in normal and control groups (433.88 ± 11.64,423.44 ±4. 70 respectively).The IA values of EGFR positive cells in experimental groups 1-4 were 198. 16 ± 34. 35, 138.00 ± 12.04,46.03 ± 6. 74, 27.22 ± 5.52 respectively, which were significantly lower than in normal and control groups (804. 74 ± 22. 52,800. 03 ± 21.96 respectively). The expression levels of EGF and EGFR could be detected in each subgroup and statistically significant differences existed in the expression of EGF and EGFR between any two groups ( P ＜ 0. 05 ). With the increase of DES dosage, the expression of EGF and EGFR was decreased, with the difference being significant amond the groups ( P ＜ 0. 05 ). Conclusion DES can influence the expression of EGF and EGFR in mice testis, which might be one of possible mechanisms effecting the development of testis.