大肠癌病人外周血,肿瘤及癌旁粘膜中胃泌素,生长抑素水平的…

对４８例大肠癌病人外周血，肿瘤及癌旁粘膜匀浆中胃泌素，生长抑素水平的ＲＩＡ测定。结果表明：癌患者平均血清胃泌素水平显著高于对照组，肿瘤切除后明显下降；分组比较表明Ｄｕｋｅｓ Ｃ期和直肠癌病人平均ＳＧ水平显著高对照组；肿瘤及癌旁粘膜中胃泌素水平很低，无法测出     

大肠癌病人外周血、肿瘤及癌旁粘膜中胃泌素、生长抑素水平的变化及其意义

对４８例大肠癌病人外周血、肿瘤及癌旁粘膜匀浆中胃泌素、生长抑素（ＳＳ）水平行ＲＩＡ测定。结果表明：癌患者平均血清胃泌素（ＳＧ）水平显著高于对照组（Ｐ＜０．０５），肿瘤切除后明显下降（Ｐ＜０．０５）；分组比较表明ＤｕｋｅｓＣ期和直肠癌病人平均ＳＧ水平显著高于对照组（Ｐ＜０．０５）；肿瘤及癌旁粘膜中胃泌素水平很低，无法测出。癌患者平均血浆ＳＳ（ＰＳＳ）水平显著高于对照组（Ｐ＜０．０５）；分组比较表明ＤｕｋｅｓＢ期和粘液腺癌病人平均ＰＳＳ水平显著高于对照组（Ｐ＜０．０５）；癌远旁粘膜（距肿瘤５～１０ｃｍ）平均ＳＳ水平高于癌组织，低于癌近旁（距肿瘤＜２ｃｍ）粘膜，三者间相差均非常显著（Ｐ＜０．０１）。ＳＧ与癌近旁ＳＳ水平间、ＰＳＳ与肿瘤ＳＳ水平间均呈非常显著之正相关（Ｐ＜０．０１）。     

胃癌癌区动－静脉血及其癌组织胃泌素变化的研究

目的：临床观察胃癌癌区动－静脉血及其癌区组织、癌旁区粘膜和外周区正常粘膜中胃泌素的变化，探讨病人体内胃泌素变化的原因和意义。方法：采用放射免疫法测定26例胃癌患者癌区动－静脉血及其癌区组织、癌旁区粘膜和外周区正常粘膜中胃泌素水平。结果：胃癌癌区静脉血中胃泌素水平显著高于癌区动脉血(P<0.05)。胃窦部癌癌旁粘膜中胃泌素水平显著高于癌区组织和外周区正常粘膜(P<0.01)，也显著高于胃体部癌癌旁区粘膜(P<0.01)。胃体部癌癌旁区粘膜中胃泌素水平显著高于癌区组织(P<0.05)。结论：胃癌组织能分泌和释放胃泌素，可能是导致静脉血中胃泌素水平升高的主要原因。癌旁区粘膜中胃泌素的增多可能在胃癌的发生发展中起重要作用。     

胃癌癌区动—静脉血及其癌组织胃泌素变化的研究

目的：临床观察胃癌癌区动－静脉血及其癌区组织、癌旁区粘膜和外周区正常粘膜中胃泌素的变化,探讨病人体内胃泌素变化的原因和意义。方法：采用放射免疫法测定２６例胃癌患者癌区动－静脉血及其癌区组织、癌旁区粘膜和外周区正常粘膜中胃泌素水平。结果：胃癌癌区静脉血中胃泌素水平显著高于癌区动脉血（Ｐ＜０．０５）。胃窦部癌癌旁粘膜中胃泌素水平显著高于癌区组织和外周区正常粘膜（Ｐ＜０．０１）,也显著高于胃体部癌癌旁区粘膜（Ｐ＜０．０１）。胃体部癌癌旁区粘膜中胃泌素水平显著高于癌区组织（Ｐ＜０．０５）。结论：胃癌组织能分泌和释放胃泌素,可能是导致静脉血中胃泌素水平升高的主要原因。癌旁区粘膜中胃泌素的增多可能在胃癌的发生发展中起重要作用。     

大肠癌组织胃泌素与增殖细胞核抗原表达的关系

目的 :研究大肠癌组织胃泌素与增殖细胞核抗原 (proliferatingcellnuclearantigen ,PCNA)表达的关系 ,以探讨胃泌素对大肠癌的促增殖作用。方法 :采用免疫组化法检测 4 8例大肠癌患者癌组织及癌旁粘膜胃泌素和PCNA的表达。结果 :大肠癌组织胃泌素阳性率为 3 9 5 % ,高分化腺癌明显高于低分化和粘液腺癌 (P <0 0 5 )。癌组织的PCNA LⅠ显著高于癌旁粘膜和正常粘膜 (P <0 0 1) ,Dukes C期和D期显著高于A期 (P <0 0 5 )。胃泌素阳性组癌组织PCNA LⅠ为 63 16± 15 14 ,胃泌素阴性组为 4 7 4 1± 13 0 4 ,两者差异显著 (P <0 0 1)。结论 :部分大肠癌细胞可能通过自分泌方式产生胃泌素 ,促进细胞的增殖     

大肠癌组织及癌旁粘膜胃泌素表达的意义

大肠癌组织及癌旁粘膜胃泌素表达的意义江春平，陈玉泉，朱建伟，沈洪薰，于秀，陈瑞新南通医学院附属医院普外科（江苏省南通市２２６００１）采用免疫组化染色对５８例大肠癌、癌旁粘膜胃泌素表达进行研究，现将研究结果报道如下：１材料和方法５８例大肠癌手术切除标本...     

胃泌素及生长抑素与大肠癌死亡受体的关系

近年来，胃泌素(GAS)、生长抑素与大肠癌发生、发展的研究已成为热点。其中胃泌素如何促进大肠癌的发生及发展，生长抑素如何抑制大肠癌的生长是重点研究方向，而死亡受体(DR)的信号途径是细胞凋亡的重要途径，现就胃泌素、生长抑素与大肠癌死亡受体之间的研究进展作一综述。     

大肠癌患者癌组织胃泌素表达与血清胃泌素水平的临床研究

目的：观察大肠癌患者血清胃泌素及肿瘤组织的ＧＡＳ表达情况。方法：采用放射免疫及免疫组化技术测定７０例大肠癌患者血清胃泌素水平。结果：胃泌素阳性表达率，中、低分化癌比高分化癌为高；粘液腺癌、印戒细胞癌、未分化腺癌较乳头状腺癌和管状腺癌为高，差异均非常显著（Ｐ＜０．０１）。结论：胃泌素阳性细胞过度表达可能是大肠癌分化不良的一个标志，且可能是肿瘤恶性程度增高的原因之一。血清胃泌素测定对诊断大肠癌灵敏度和特异性不高，但由于其升高为癌组织胃泌素阳性表达所致，与肿瘤的分化程度、恶性度、大小及分期密切相关。大肠癌病人术前血清胃泌素水平增高，则提示其肿瘤恶性程度较高，分期较晚，预后不良。     

大肠癌旁膜细胞增殖模式与术后复发机制的探讨

目的：探讨大肠癌旁粘膜细胞模式变化与肿瘤术后复发的关系。方法：用免疫组化染色方法对７８例大肠癌标本的癌组织、癌旁粘膜及１２例正常大肠粘膜进行Ｐ５３蛋白、增殖细胞核抗原（ＰＣＮＡ）检测，并进行随访。结果：癌组织ＰＣＮＡ高表达明显高于癌旁，癌旁ＰＣＮＡ高表达与隐窝增殖区扩大、上移，明显影响术后第１年局部复发死亡率，癌组织Ｐ５３蛋白阳性表达率６１．５４％，癌旁为２６．９２％，癌及癌旁Ｐ５３阳性表达不影响     

大肠癌旁粘膜上皮核DNA分析

 采用显微分光光度计对20例大肠癌及癌旁粘膜上皮细胞核DNA含量测定，结果表明，正常大肠粘膜和静止小淋巴细胞为近2C，癌细胞核DNA含量明显高于正常和癌旁粘膜；癌旁粘膜上皮细胞核DNA明显增高，与正常粘膜相此较，2C细胞较少，4C和超过4C细胞增多，说明癌旁粘膜上皮细胞代谢旺盛，增殖活跃，提示可能是一种癌前状态。     

The study of colorectal carcinoma associated antigen lea in clinical pathological diagnosis

The colorectal cancer is a disease which seriously threatens human being[1]. Early diagnosis and early treatment are very important to improve the life quality and the survival rate. CEA is a serum tumor marker for colorectal cancer first discovered by Gold and Freedman in 1965[2]. It is a glycoprotein of about 180 KD and a tumor-associated antigen extracted from colon cancer and fetal intestine. It is an extensively used broad-spectrum tumor marker at present. In 1986 Song Jin-dan, using …     

Positive correlation of insulin-like growth factor-II with proliferating cell index in patients with colorectal neoplasia.

BACKGROUND: Insulin-like growth factor-II (IGF-II) stimulates cell proliferation and is considered a potential risk factor for colorectal cancer. Tumor levels of IGF-II seem to positively correlate with colorectal cancer cell proliferation. This investigation examined the association of circulating IGF-II to the proliferating cell index (PCI) of tumor and matched normal mucosa in patients with colorectal neoplasia. METHODS: Circulating IGF-II level (ng/mL) was determined in the peripheral blood plasma by ELISA. The proliferating cells in tumor or matched normal mucosa were immunohistochemically stained using the primary antibody against Ki-67. Computer image analysis was used and PCI was expressed as the percentage of Ki-67-positive cells/total counted cells. RESULTS: Sixty-four patients were evaluated; 45 had colorectal neoplasia (27 males/18 females; mean age, 66.8 +/- 11.8 years) and 19 had hyperplastic polyps (6 males and 13 females; mean age, 58.4 +/- 14.4 years). Among patients with colorectal neoplasia, blood IGF-II levels were positively correlated with PCI in the matched normal mucosa (r = 0.46, P < 0.05) but not in the tumor. In patients with hyperplastic polyps, blood IGF-II levels were not correlated with the PCI in the polyps. Blood IGF-II levels were higher in colorectal cancer patients with Dukes' C/D stage (P < 0.01) or with positive lymph nodes (P < 0.01). CONCLUSION: Circulating IGF-II positively correlated with PCI in normal colonic mucosa of patients with colorectal neoplasia, suggesting that IGF-II may have a role in initiating the carcinogenic pathway by stimulating cell proliferation. Blood IGF-II was increased in advanced colorectal cancer, indicating that it might enhance colorectal cancer progression and be a useful marker of poor prognosis.     

肿瘤相关抗原LEA的表达及其在病理诊断中的意义

背景与目的：目前,大肠癌诊断的肿瘤标志物如CEA（carcinoembryonic antigen)等,对大肠癌患者的特异性不强,用于早期诊断价值不高,研究表明LEA（large external antigen)对大肠癌组织有极好的特异性,本研究应用抗LEA单抗ND－1和抗CEA单抗对大肠癌组织的对比,探讨LEA在大肠癌中的表达及其临床病理诊断意义。方法：采用免疫组织化学S－P法检测170例大肠癌组织和100例大肠非癌组织（41例大肠腺瘤,32例癌旁粘膜,27例正常粘膜）LEA与CEA的表达,结果：LEA在高、中、低分化腺癌组织中表达阳性率分别为100％,83．1％和51．8％,而CEA分别为93．8％,92．3％,和70．4％,LEA大肠腺瘤,癌旁粘膜和正常粘膜中表达阳性率分别为75．6％,53．1％和14．8％,而CEA分别为82．9％,62．5％和40．7％,LEA对高分化腺癌表现出更高的选择性（P＜0．01）,CEA单抗对高、中、低分化腺癌选择性相似（P＞0．05）,与CEA相比,LEA在非癌组织中表达的阳性率较低（P＜0．05）,LEA与CEA的表达除在正常为膜外均有显著的相关性,LEA与CEA在组织学上诊断大肠癌的灵敏度分别为84．1％和88．8％,而特异度分别为48％和35％,结论：LEA可能是一种与细胞分化,侵袭能力相关的肿瘤抗原,LEA可用于大肠癌恶性程度判断的参考指标,是具有临床实用价值的肿瘤生物标记物。     

EXPRESSION OF IL-2R ON PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH COLORECTAL CANCER AND ITS CLINICAL SIGNIFICANCE

In recent years it has been noted that the levels of serum soluble interleukin-2 receptor (sIL-2R) in patients with malignancy were abnormally elevated and the levels of sIL-2R might be considered as an index to monitor patient's condition and assess treatment effects. But in medical literature it was reported that the levels of membrane interleukin-2 receptor (CD25) in peripheral blood lymphocytes (PBL) in patients with malignancy were various, some were elevated, some were dropped.[1] Th…     

Overexpression of gastrin and c-met protein involved in human gastric carcinomas and intestinal metaplasia

Many studies have investigated the expression of c-met and c-erbB2 protein in human gastric adenocarcinomas, but the expression of gastrin protein in human gastric cancer and the relationship between gastrin and c-met are unknown. We have constructed a tissue microarray containing 408 formalin-fixed and paraffin-embedded human tissue blocks, including tissues containing intestinal metaplasia (IM, n=72) and primary tumors (n=232), as well as normal gastric mucosa (n=104) from patients with gastric cancer. Immunohistochemistry (IHC) was used for detecting gastrin, c-met and c-erbB2 proteins. Gastrin was detected in 13.5% (7/52) and c-met in 15.3% (11/72) of IM cases. In gastric carcinomas, 48.4% (103/213) of cases expressed gastrin, 68.8% (148/215) expressed c-met, and 5.5% (11/200) expressed c-erbB2. Gastrin and c-met protein expression were significantly higher in gastric tumor tissue than in IM (P<0.0001). Overexpression of c-erbB2 protein was detected in gastric carcinomas but not in normal gastric mucosa (P<0.05). Expression of gastrin and c-met protein was associated (P<0.01), but no significant difference was found on the changes of gastrin, c-met and c-erbB2 expression in gastric cancer with tumor stage, grade of differentiation or tumor type. These results indicate that gastrin and c-met play a role in the early process during malignant transformation of the gastric mucosa.     

Expression of p53, Ki-67 and c-Myc Proteins is Predictive of the Surgical Molecular Margin in Colorectal Carcinoma

Surgical resection is the mainstay of treatment for colorectal carcinoma, however, the overall survival is modest due to frequent local recurrence from residual cancer cells after “curative” resection. Therefore, the status of surgical margin (tumor free or positive) has a significant influence on patient’s survival. The difference in molecular profile between mucosa neighboring tumor lesions and remote area (surgical margin) may aid in evaluating resection status. 44 colorectal tumor tissues with corresponding adjacent non-neoplastic mucosa (within 3 cm from tumor tissues), and 110 tumor tissues with corresponding surgical margin mucosa (5 cm from tumor tissues) were randomly collected, fixed in 10% formalin and followed by embedding in paraffin. And the expression of p53, Ki-67 and c-Myc were investigated by tissue microarray (TMA) and immunohistochmistry. The expression of p53, Ki-67 and c-Myc were decreased in both adjacent non-neoplastic mucosa and mucosa of surgical margin, comparing to their expression in corresponding cancer cells. Furthermore, the expression of these proteins in mucosa of remote area (surgical margin) was significantly lower than those adjacent to tumor lesions. The expression of p53, Ki-67 and c-Myc in mucosa can be used as molecular marker for assessing surgical margin status in colorectal carcinoma.     

Prognostic significance of gastrin expression in patients undergoing R0 gastrectomy for adenocarcinoma

Background Gastrointestinal (GI) hormones regulate several GI functions, including the proliferation and repair of normal mucosa, and hormone receptors may therefore be implicated in the growth, invasion, and metastasis of cancers of the GI tract. The aim of this study was to determine the cellular distribution of gastrin in intestinal-type gastric cancers, and to determine its relationship to outcomes after R0 gastrectomy. Methods Eighty-six consecutive patients undergoing R0 gastrectomy for adenocarcinoma were studied. Normal gastric mucosa and tumor were stained for gastrin and their specific cellular distribution was determined. Results The duration of survival of patients whose tumors exhibited well-differentiated gastrin-positive tumor (GPT) cells (n = 12) was significantly poorer than that of patients whose tumors were GPT-negative (5-year survival, 30% vs 54%; P = 0.037). Patients with GPT-positive intestinal-type gastric cancer (5 of 47 patients) had the poorest survival of all (median, 14 months; 5-year survival, 0%; P = 0.006). In a multivariate analysis, only lymph node metastases (hazard ratio [HR], 2.13; 95% confidence interval [CI], 1.2 to 3.79; P = 0.01) and the presence of GPT cells (HR, 6.61; 95% CI, 1.74 to 25.09; P = 0.01) were independently and significantly associated with durations of survival in patients with intestinal-type gastric cancer. Conclusion The presence of GPT cells in patients with gastric adenocarcinoma is a significant and independent prognostic indicator. An original article presented in the Moynihan Prize session at the Associations of Surgeons of Great Britain and Ireland, Edinburgh, May 2006.     

肿瘤相关抗原在人大肠癌中的表达

 目的 应用ND-1单抗和抗CEA单抗对大肠癌细胞的标记情况对比，探讨LEA在大肠癌中的表达及意义。方法 采用流式细胞仪和免疫细胞化学染色检测大肠癌细胞系LEA和CEA的表达。并采用间接ELISA法测定LEA和CEA单抗对大肠癌细胞系的特异性。应用免疫组化检测其在大肠癌组织中的表达。结果 流式细胞仪检测显示，LEA抗原在CCL-187、CX-1、CLone A和CCL-229细胞表达的阳性峰平均荧光强度呈递减趋势，并且强于CEA的表达量（P〈0．01）。LEA在高分化大肠癌细胞系CCL187和CX-1高度表达，并且低侵袭大肠癌细胞系CCL-187和CX-1 LEA表达量高于高侵袭大肠癌细胞系CLone A和CCL-229（P〈0．01）。ELISA检测表明，与抗CEA单抗相比，ND-1单抗对大肠癌细胞具有很强的特异性结合力（P〈0．01）。LEA的表达阳性率随大肠腺癌组织分化程度降低而下降（P〈0．01），而且对高分化腺癌表现出更高的选择性。抗CEA单抗对高、中、低分化癌选择性相似（P〉0.05）。结论 LEA是更加特异的高分化大肠癌相关抗原。LEA可能与癌细胞的分化程度、侵袭力、恶性程度有关，LEA可作为早期诊断和判断大肠癌恶性程度的有用指标。