综合干预对重庆市某儿童福利院婴儿体智能发育影响的研究

目的 考察综合干预对重庆市某儿童福利院婴儿体智能发育的影响.方法 将来自福利院的48例8～9月龄婴儿随机分为对照组与干预组.干预组予游泳、抚触、早教与多感官刺激训练;对照组不做干预.干预前后采用格赛尔(Gesell)发育量表分别对两组婴儿的动作能、应物能、语言能、应人能4个能区进行评估.结果 1个月后评估表明:对照组,语言能和应物能所提高(P＜0.05),动作能、应物能及总体指数相比无显著差异.干预组动作能、应物能、语言能、应人能及总体指数均较前显著提高(P＜0.01).干预组各能区指数增高值均较对照组显著提高(P＜0.01).结论 对福利院儿童提供必要的丰富环境与疗育措施非常有效和重要.     

早期教育对不同气质类型幼儿发育商的影响研究

目的探讨早期教育对不同气质类型幼儿发育商的影响。方法将正常幼儿按气质类型分为平易型、困难型及启动缓慢型,按照随机兼自愿的原则将各气质类型的幼儿分为早教组108例和对照组96例,早教组由医生和幼师直接进行教育训练,并改善其父母对早教的认识和态度;对照组仅进行常规体检。半年后用盲法对两组小儿进行发育商的测评。结果早教训练后,两组平易型气质幼儿在大运动、精细动作、应人能方面差异有显著性意义(P<0.05)。两组困难型气质幼儿在精细动作、语言、应物和应人能方面差异均有显著性意义(P<0.05)。两组启动缓慢型幼儿在大运动、精细动作、语言、应物和应人能方面差异均有显著性意义(P<0.05)。结论早期教育对困难型和启动缓慢型幼儿智能发育的积极影响更为显著,需要对此类婴幼儿进行恰当的早期教育训练,以利于其智力潜能的充分发挥。     

游泳与抚触对婴儿生长发育影响的效果观察

目的：探讨游泳与抚触对婴儿生长发育影响的效果。方法：对40名新生儿进行游泳及抚触并设对照组，记录2组儿童1、3、6个月时的身长、体重、头围均值及发育指标。每3个月进行1次发育指标评定和婴幼儿智能发育量表测试。结果：2组婴儿3、6个月时体重、身长发育均值比较，差异有统计学意义（P〈0．05）；各能区发育以大运动、精细运动、个人交往能区发育商差异有统计学意义（P〈0．01）；智力发育指数和运动发育指数比较，差异有统计学意义（P〈0．01）。结论：游泳与抚触对婴和生长发育有促进作用。     

水疗对正常婴儿智能发育影响的研究

目的:观察水疗对正常婴儿智能发育和体格发育的影响。方法:观察157例自然分娩足月正常新生儿,随机分为水疗组和对照组.从出生后24h开始对新生儿进行水疗,水疗前后给予抚触.1次/d,每次10min～15min,共60d。60d时应用0岁～3岁小儿精神发育检查表专人进行测评,得出智能五大能区及在此基础上智商的得分;对两组婴儿2月龄时体重进行了测定,并进行统计学处理。结果:水疗组婴儿在智能五大能区(大运动、精细动作、适应能力、语言、情绪和社会行为)方面得分均有提高,智商也有提高,与对照组相比,差异均有显著性(P<0.05,P<0.01),水疗组婴儿体重高与对照组,差异有显著性(P<0.05)。结论:水疗可提高正常婴儿的智商及婴儿各方面的发育,对婴儿体格发育亦有良性的促进作用。     

148例婴儿游泳及抚触的效果观察

目的探讨游泳及抚触对婴儿神经、心理发育作用,方法对148名正常新生儿进行游泳、抚触并观察婴儿神经、行为发育完成的月份,应用Gesell发育量表和婴幼儿智能发育量表(CDCC)进行测试。结果观察组婴儿神经、行为发育月份提前,各能区发育以大运动,精细运动,个人交往能区发育商均较对照组提前(P<0.01),智能发育商数(MDI)和运动发育商数(PDI)比较,差异均有显著性意义(P<0.01),结论游泳与抚触活动对婴儿神经、心理发育有促进作用。     

婴儿水育结合抚触对正常婴儿智能发育的影响

目的 观察水育结合抚触对婴儿智能发育和体格发育的影响.方法 观察207例自然分娩足月正常新生儿,随机分为水育加抚触组和对照组.从出生后24h开始对新生儿进行水育,水育前后给予抚触,1次/d,每次10m i n～15m i n,共60d.60d时应用0岁～3岁小儿精神发育检查表专人进行测评,得出智能五大能区及在此基础上智商的得分;对两组婴儿2月龄时体重进行了测定,并进行统计学处理.结果 水育加抚触组婴儿在智能五大能区(大运动、精细动作、适应能力语言、情绪和社会行为)方面得分均有提高,智商也有提高,与对照组相比,差异均有显著性(P<0.05,P<0.01),水育加抚触组婴儿体重高于对照组,差异有显著性(P<0.05).结论 水育加抚触提高正常婴儿的智商及婴儿各方面的发育,对婴儿体格发育亦有良性的促进作用.     

孕母慢性淋巴细胞性甲状腺炎对婴儿智能发育的影响

目的：探讨孕母患慢性淋巴细胞性甲状腺炎对婴儿的影响。方法：通过浙江省新生儿疾病筛查网络系统，从2001年7月至2003年6月对21例母亲患慢性淋巴细胞性甲状腺炎的婴儿进行随访，观察其甲状腺功能情况，并采用Gesell发育量表对其智能发育进行跟踪调查。结果：21例婴儿中甲状腺功能正常8例，高TSH血症11例，先天性甲低2例，与同期健康母亲的新生儿相比差异有显著性（P〈0．01）；孕母患慢性淋巴细胞性甲状腺炎的婴儿其应物能、应人能、粗动作能、细动作能发育均落后，与健康孕母的婴儿比较，差异有显著性（P〈0．05）。结论：孕母患慢性淋巴细胞性甲状腺炎对婴儿甲状腺功能及智能发育有影响。     

新生儿游泳对睡眠及体重的影响

目的:探讨新生儿泳疗对睡眠及体重的影响,为泳疗提供科学依据。方法:本文将160例婴儿随机分为观察组和对照组,各80例。入组对象排除来自孕母或自身可能对新生儿神经行为发育造成一定影响的危险因素。观察两组婴儿体重增长、睡眠情况。结果:观察组的新生儿游泳中体重增长、睡眠质量情况明显高于对照组。结论:泳疗对新生儿神经行为发育确有促进作用,值得推广。     

不同时期开始抚触对婴儿智能发育的研究

目的　探讨不同时期开始抚触对婴儿智能发育的影响。方法　将正常足月儿随机分为 3组 ,出生第 2天开始抚触者为Ⅰ组 ,满月时开始抚触者为Ⅱ组 ,未抚触者为Ⅲ组。分别在 3月龄和 9月龄查体时用Gesell量表测试比较。结果　Ⅰ组和Ⅱ组婴儿 3月龄、 9月龄时的动作能、应物能、应人能与Ⅲ组间差别有显著性意义 (P <0 0 5 ) ,Ⅰ组与Ⅱ组间在 3月龄时的动作能、应人能差别有显著性意义 (P <0 0 5 ) ,至 9月龄时应物能间差别有显著性意义 (P <0 0 5 )。结论　在新生儿早期实施抚触能更有益地促进婴儿神经、心理良好发育并产生延续效应。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.