Lactate and succinate dehydrogenase activity in blood lymphocytes of aggressive and submissive male mice

The influence of social status on some immunological characteristics in experimental animals was studied (sensory contact model). Lactate dehydrogenase activity in blood lymphocytes changed similarly in aggressive and submissive mice probably due to social stress. The activity of succinate dehydrogenase changed differently and correlated with the type of social behavior. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 129, No. 1, pp. 53–55, January 2000     

Altered activity of respiratory chain enzymes in mitochondria of peripheral blood lymphocytes after irradiation in rats

The effect of ionizing radiation on the activity of succinate dehydrogenase (complex II) and succinate-cytochrome C-oxidoreductase in peripheral blood lymphocytes is studied on rats exposed to whole-body γ-irradiation in doses of 9.5–10.5 Gy. On day 5 after irradiation, when the number of lymphocytes is sharply reduced, enzyme activity in the remaining population is found to be reliably increased. These changes are not related to biological cycles. It is assumed that most of the survivors after high-dose irradiation are the cell populations maintaining a high level of oxidative phosphorylation in mitochondria. Translated fromByulleten' Eksperimental'noi Giologii i Meditsiny, Vol. 120, N ^o . 8, pp. 158–159, August, 1995 Presented by D. S. Sarkisov, Member of the Russian Academy of Medical Sciences     

Androstenedione conversion in human peripheral blood lymphocytes

The ability of human peripheral blood lymphocytes to convert the androgenic steroid metabolite androstenedione diminishes in women over 45 years old or during menopause. In patients with breast cancer in menopause the ability of lymphocytes to convert androstenedione is enhanced compared with the control. The intensity of conversion in circulating lymphocytes correlates with the blood concentration of sex steroids. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N ^o 5, pp. 517–519, May, 1994 Presented by A. N. Klimov, Member of the Russian Academy of Medical Sciences     

Count and heterogeneity of human fetal blood lymphocytes in the course of fetal development

Blood specimens from 89 human embryos and fetuses were analyzed by immunological methods during gestation weeks 4–40. All formed elements circulate in fetal blood starting from week 13 of gestation. Lymphocytes predominate among blood leukocytes, because normally lymphocytosis is an inherent, feature of blood. T cells and their subpopulations: active T lymphocytes, Tγ lymphocytes, and theophylline-sensitive and risistant, cells are present. B lymphocytes are represented by cells with immunoglobulins M and G, zero cells, and large granular lymphocytes. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 126., No. 9, pp. 331–333, September, 1998     

Circadian and seasonal variations in blood lymphocyte dehydrogenase activities in women with secondary immunodeficiency and acute nonspecific gynecological inflammations

Development of secondary immunodeficiency in patients with acute nonspecific inflammatory diseases of the uterine appendages is associated with disorders in chronobiological characteristics of energy metabolism in blood lymphocytes. The differences in the enzyme activities in patients and donors are observed in the evening during all seasons of the year; seasonal variations are typical only of the morning enzyme spectrum. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 124, No. 5, pp. 576–578, May, 1998     

Increased interleukin-2 production by human peripheral blood lymphocytes treated with low doses of dexamethasone

Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 116, N ^o 7, pp. 68–70, July, 1993.     

Functional activity of spleen and peripheral blood lymphocytes in stress-induced immunodepression

Study of lymphoid organs and T and B cells in white rats shows that long-term stress causes progressive suppression of the immune response. Blockade of steroid hormone synthesis in the adrenal cortex prevents the development of immunodepression, implying a protective effect of such a blockade against stress-related secondary immunodeficiency. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 122, No. 7, pp. 64–68, July, 1996     

Characterization of benzodiazepine receptors of human lymphocytes

It is demonstrated that benzodiazepine binding sites on human peripheral blood lymphocytes are the peripheral type receptors. The binding sites for the selective agonist³H-Ro 5-4864, but not for the antagonist³H-PK 11195, are completely inactivated by freezing-thawing of lymphocytes. The binding of³H-Ro 5-4864 to intact lymphocytes is activated by GABA and (+)baclofen. It is shown that the selective ligands Ro 5-4864 and PK 11195 bind to different subtypes of benzodiazepine receptors on human peripheral blood lymphocytes. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 126, No. 10, pp. 405–408, October, 1998     

Cytogenetic anomalies in cultured human lymphocytes exposed to halogen analogs of thymidine

Allocyclic chromosomes and monochromatid breaks are observed during testing of a number of halogen analogs of thymidine. The first effect depends directly and the second inversely on the molecular weight of the analog. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N ^o 9, pp. 326–328, September, 1995 Presented by N. P. Bochkov, Member of the Russian Academy of Medical Sciences     

Is the abnormal reaction of lymphocytes of patients with atopic bronchial asthma to adenosine a component of pathogenesis or a compensatory reaction?

After a 30-min incubation in the presence of adenosine, the suppressor activity of peripheral blood lymphocytes from healthy subjects decreases as well as the count of CD4⁺ cells, while the count of CD8⁺ cells increases. The suppressor activity of peripheral blood lymphocytes from patients with atopic bronchial asthma and the count of CD8⁺ cells increase after a 16-h incubation with adenosine, while a 30-min incubation enhances the expression of CD4 antigen. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 122, No. 10, pp. 474–477, October, 1996     

Effect of cortisol and pertussis toxin on the cAMP concentration in human lymphocytes

It is demonstrated that pertussis toxin and hydrocortisone potentiate the adenosine-induced rise of the cAMP concentration in lymphocytes. Hydrocortisone elicits an immediate (for the simultaneous addition of adenosine and cortisol) and reversible effect. The effect of pertussis toxin has a latency and is irreversible. Added together, these agents exert no cumulative effect. It is assumed that hydrocortisone and pertussis toxin have the same target — The inhibiting regulatory protein G_i. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N ^o 2, pp. 171–173, February, 1995 Presented by P. V. Sergeev, Member of the Russian Academy of Medical Sciences     

Frequency and phase correlation of rhythms of dihygroorotate dehydrogenase activity in rat peripheral blood lymphocytes and solar activity rhythms and wave periods of planets

The common variation pattern and the synchronous increases of enzymatic and solar activity are explained by the identical frequencies of the bio- and heliorhythms and by the strict phase coordination of the latter. It is shown to be possible to predict enzymatic activity according to the sum of the resonance heliorhythms. The wave genesis of the resonance between enzymatic and solar fluctuations is confirmed by the fact that the periods of the bio- and heliorhythms are the same as the wave periods of planets. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N ^o 9, pp. 294–298, September, 1995 Presented by F. I. Komarov, Member of the Russian Academy of Medical Sciences     

Determination of fructose 2,6-bisphosphate in human lymphocytes is a potential laboratory test in diabetology

The conventional micromethod of fructose 2,6-bisphosphate (F-2,6-P₂) determination is optimized for human peripheral blood lymphocytes. The F-2,6-P₂ content in lymphocytes of healthy subjects is 0.8±0.2 pmol/10⁶ cells or 6.7±1.1 pmol/mg protein. Preliminary findings show a decrease in the F-2,6-P₂ content in lymphocytes from patients with severe diabetes mellitus. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 122, No. 9, pp. 341–344, September, 1996     

Expression of CD95 on peripheral blood lymphocytes in patients with autoimmune diseases and neoplasms

CD95 expression on peripheral blood lymphocytes in patients with neoplasms was higher than in patients with autoimmune disorders. Apoptosis of T cells increased during tumor growth. The data suggest that neoplasms are accompanied by more severe immune dysfunction than autoimmune disorders. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 130, No. 9, pp. 324–326, September, 2000     

Rosette formation by human,guinea pig,and rat lymphocytes with spermatozoa

Cells forming rosettes with homologous or heterologous spermatozoa were found in the thymus, spleen, and bone marrow of sexually mature guinea pigs and of 14–30-week-old human fetuses, and also in the peripheral blood of men suffering from sterility. On the development of autoimmune orchitis after measured trauma to the testis in rats or after immunization of guinea pigs with testicular tissue homogenate mixed with Freund's complete adjuvant, cells forming rosettes with spermatozoa were found to appear in the spleen and thymus of the rats, and their number in the lymphoid organs of the guinea pigs increased. These procedures had no effect on the number of cells forming rosettes with sheep red blood cells in the lymphoid organs of rats and guinea pigs. The possible use of this newly discovered ability of human and animal lymphocytes to form spontaneous and immune rosettes with spermatozoa as a means of assessing the degree of differentiation of lymphocytes and of their sensitization to spermatozoal antigens in cases of disturbance of spermatogenesis of autoimmune nature is discussed.Laboratory of Human Embryonic Histogenesis, Institute of Human Morphology, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR A. P. Avtsyn.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 83, No. 4, pp. 464–467, April 1977.     

Individual variations of the immune response in BALB/c mice

The nature and the causes of variations of the immune response to thyroid hormones are analyzed in BALB/c mice. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N ^o 1, pp. 80–82, January, 1995 (Presented by Yu. A. Romanov, Member of the Russian Academy of Medical Sciences)     

Expression of CD95 on peripheral blood lymphocytes in patients with autoimmune diseases and neoplasms

CD95 expression on peripheral blood lymphocytes in patients with neoplasms was higher than in patients with autoimmune disorders. Apoptosis of T cells increased during tumor growth. The data suggest that neoplasms are accompanied by more severe immune dysfunction than autoimmune disorders. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 130, No. 9, pp. 324–326, September, 2000     

Effect of ricin and its B-subunit on calcium responses in human lymphocytes

The effect of ricin and its B-subunit on cytoplasmic Ca²⁺ concentration in human lymphocytes was studied using a fluorescence probe. Both agents dose-dependently increased cytosolic free Ca²⁺ by stimulating its release from of thapsigargin-sensitive store. It is suggested that this effect is associated with the increase in cytosolic content of inositol-1,4,5-trisphosphate. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 129, No. 3, pp. 274–276, March, 2000     

Cholesterol level in peripheral blood lymphocytes: Relationship with ischemic heart disease in patients with various forms of hyperlipidemia

Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol.116, N ^o 11, pp. 476–479, November 1993